Golden Gate Tn10

The Golden Gate Tn10 is begun again and the transformation of DH5α with this new product is also begun again. After that the cell growth, the plasmid is purified.

OriVR6Kgamma muted

Each plasmid pNK2 (2) purified is digested with XbaI to verified the mutation.
Gel
The site XbaI in Ori have been muted. There are 2 bands instead of 3

Sep 08