Results of sequencing to Tn10

The results of sequencing to Tn10 with primer biobrick is received. The sequence corresponding to Tn10 in the plasmid to 99,9%. The 0,1% corresponding at the base N. DH5α pir is transformed with the product of Golden Gate Tn10.

Transformation with pNK2 (2)

The DH5α pir transformed with pNK2 (2) have growth. Some colonies are resuspended in 4mL to LB.

Sep 04