Harvard BioDesign/18 June 2014

From 2014.igem.org

Miniprepped pHBD 13, 15, 16, 18 (13 and 18 biological replicates since concentrations from previous day’s miniprep were very low) pHBD20 didn’t grow

Transformed chromoproteins: pHBD 32, 33, 34, 35 from iGEM 2014 kit plate (blue red and green)

Ordered eforred, amilGFP, and aeblue from the regristry

Mixing oil and water and putting in autoclave was unsuccessful - Added Tween 20 as a surfactant to see if this would be sufficient to make the water and oil mix

Went to a talk by Elias ***** about bioinformatics - Used MATLAB