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From 2014.igem.org

Some plates didn’t have individual colonies; just smears. This is likely due to the fact that we added too little antibiotic to our plates. Instead of adding 250 µl of CM, we added 25 µl. So, we threw out our plates and made more.

Miniprepped pHBD 19, 11, 4, 18, 13, 10, 9, 12, 14.

We also designed Gibson primers to put the BioBricks in the appropriate plasmids.

We attempted to replate the BioBricks that we couldn’t pick. This time, we made serial dilutions so that the final concentration relative to the number of cells we got from the stab was 1:10,000.

Activity Log Put turbo cells in larger flask with LB+ carb to grow up Autoclaved gum arabic 3 g of gum arabic + 15 mL of LB and carb Made solutions of gum arabic + LB + cells at varying concentrations of gum arabic 200 g/L concentration, 20 g/L concentration, 2g/L concentration Cells added when their OD read 0.95 This is a little high, but we continued on

Results of OD “Comparison” - Used the gum arabic and LB concentrations corresponding to each concentration of gum arabic+ LB+ cells

LB Control 0.843

200 g/L -0.491, -1.18

20 g/L 0.672

2 g/L 0.780