Team:Tuebingen/Team/Attributions
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The project idea - the conversion of blood types from A, B, or AB to O - and the overall system design were developed by the team members themselves without outside help. However, the feasibility of the project draft was then discussed with our instructors, Professor Dr. Ralf-Peter Jansen and Dr. Elisabeth Fuss. | The project idea - the conversion of blood types from A, B, or AB to O - and the overall system design were developed by the team members themselves without outside help. However, the feasibility of the project draft was then discussed with our instructors, Professor Dr. Ralf-Peter Jansen and Dr. Elisabeth Fuss. | ||
</p> | </p> | ||
- | |||
<p> | <p> | ||
Biosafety concerns for potential experiments with human blood were also discussed with Professor Dr. Jansen since the experiments would have taken place in his laboratory. Permission for working with tested blood conserves was granted by Dr. Michael Kittel from the office of biological safety at the university of Tübingen. | Biosafety concerns for potential experiments with human blood were also discussed with Professor Dr. Jansen since the experiments would have taken place in his laboratory. Permission for working with tested blood conserves was granted by Dr. Michael Kittel from the office of biological safety at the university of Tübingen. | ||
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<h3>Lab supplies</h3> | <h3>Lab supplies</h3> | ||
- | <p>We have ordered most consumables and reagents ourselves without the help or advice of instructors or other scientists. Some reagents like Bacto-Yeast Extract were obtained from Professor Dr. Jansen's stock.</p> | + | <p> |
+ | We have ordered most consumables and reagents ourselves without the help or advice of instructors or other scientists. Some reagents like Bacto-Yeast Extract were obtained from Professor Dr. Jansen's stock. | ||
+ | </p> | ||
<h3>Lab and lab hardware</h3> | <h3>Lab and lab hardware</h3> | ||
- | <p>Most experiments were carried out at the team's laboratory at the IFIB ( | + | <p> |
+ | Most experiments were carried out at the team's laboratory at the IFIB (Interfaculty Institute of Biochemistry) in Tübingen. The team's laboratory was kindly provided by Professor Ralf-Peter Jansen. Peptide synthesis and in conjunction HPLC and ESI were performed at the laboratory of the Schwarzer group at the IFIB. For practical reasons one team member carried out plasmid preparations at University of Tübingen's ZMBP (Center for Plant Molecular Biology). MALDI was performed at the Kalbacher lab in Tübingen. All team members were introduced to the handling of complex instruments by the instructors of the team or (when necessary) by the host lab's workers. After this introduction all lab hardware was operated as autonomously as possible by team members. | ||
+ | </p> | ||
<h3>DNA-sequencing</h3> | <h3>DNA-sequencing</h3> | ||
- | <p>All DNA-sequencing was performed by | + | <p> |
+ | All DNA-sequencing was provided and performed by GATC-biotech. Sequencing results were evaluated by the team members themselves. | ||
+ | </p> | ||
<h3>Lab work</h3> | <h3>Lab work</h3> | ||
- | <p>All lab work was conducted by the team members themselves. Neither the team's instructors nor workers of | + | <p> |
- | + | All lab work was conducted by the team members themselves. Neither the team's instructors nor workers of our host lab have performed one single experiment described in this wiki. External influence on lab work was restricted to advice and (at most) practical demonstrations of methods and the operation of machines. | |
- | + | </p> | |
</div> | </div> | ||
Revision as of 14:42, 17 October 2014
Attributions
Project idea
The idea for the conversion of blood types from A, B, or AB to O was drafted by the team members themselves, although research on this subject had been done before, the idea for the topic of our project emerged independently.
System design
The project idea - the conversion of blood types from A, B, or AB to O - and the overall system design were developed by the team members themselves without outside help. However, the feasibility of the project draft was then discussed with our instructors, Professor Dr. Ralf-Peter Jansen and Dr. Elisabeth Fuss.
Biosafety concerns for potential experiments with human blood were also discussed with Professor Dr. Jansen since the experiments would have taken place in his laboratory. Permission for working with tested blood conserves was granted by Dr. Michael Kittel from the office of biological safety at the university of Tübingen.
All part and primer design was performed by student team members.
Lab supplies
We have ordered most consumables and reagents ourselves without the help or advice of instructors or other scientists. Some reagents like Bacto-Yeast Extract were obtained from Professor Dr. Jansen's stock.
Lab and lab hardware
Most experiments were carried out at the team's laboratory at the IFIB (Interfaculty Institute of Biochemistry) in Tübingen. The team's laboratory was kindly provided by Professor Ralf-Peter Jansen. Peptide synthesis and in conjunction HPLC and ESI were performed at the laboratory of the Schwarzer group at the IFIB. For practical reasons one team member carried out plasmid preparations at University of Tübingen's ZMBP (Center for Plant Molecular Biology). MALDI was performed at the Kalbacher lab in Tübingen. All team members were introduced to the handling of complex instruments by the instructors of the team or (when necessary) by the host lab's workers. After this introduction all lab hardware was operated as autonomously as possible by team members.
DNA-sequencing
All DNA-sequencing was provided and performed by GATC-biotech. Sequencing results were evaluated by the team members themselves.
Lab work
All lab work was conducted by the team members themselves. Neither the team's instructors nor workers of our host lab have performed one single experiment described in this wiki. External influence on lab work was restricted to advice and (at most) practical demonstrations of methods and the operation of machines.