Team:Tuebingen/Notebook/Protocols/miniprep

From 2014.igem.org

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   <li> Store at – 20 °C.</li>
   <li> Store at – 20 °C.</li>
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<h4>Kits</h4>
<h4>Kits</h4>
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Revision as of 23:10, 14 October 2014


Preparation of plasmids on a small scale

Procedure

  1. Pick a single colony from a plate, give it in a a 15 ml Falcon-tube with 10 ml LB-medium and incubate at 37 °C and overnight.
  2. Pelletize 3 ml of the overnight-culture and discard the supernatant.
  3. Continue according to the Quiagen QIAprep ® Spin Miniprep Kit Manual or the Biozyme EasyPrep ® Pro Plasmid Miniprep Kit Manual.
  4. Store at – 20 °C.

 

Kits

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