Team:Tuebingen/Notebook/Protocols/Ni NTA Buffers

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Protocols

Ni NA buffers

Lysis buffer (pH = 8)

50 mM NaH2PO4
300 mM NaCl
10 mM Imidazole

 

Washing buffer (pH = 8)

50 mM NaH2PO4
300 mM NaCl
20 mM Imidazole

 

Elution buffer (pH = 8)

50 mM NaH2PO4
300 mM NaCl
250 mM Imidazole

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