Team:Tuebingen/Notebook/Protocols/Ni NTA Buffers

From 2014.igem.org

Revision as of 15:26, 17 October 2014 by SvenB (Talk | contribs)


Protocols

Ni NA buffers

Lysis buffer (pH = 8)

50 mM NaH2PO4
300 mM NaCl
10 mM Immidazol

 

Washing buffer (pH = 8)

50 mM NaH2PO4
300 mM NaCl
20 mM Immidazol

 

Elution buffer (pH = 8)

50 mM NaH2PO4
300 mM NaCl
250 mM Immidazol

Retrieved from "http://2014.igem.org/Team:Tuebingen/Notebook/Protocols/Ni_NTA_Buffers"