Team:Tuebingen/Notebook/Protocols/Ni NTA Buffers

From 2014.igem.org

(Difference between revisions)
(Created page with "{{:Team:Tuebingen/Templates/TueWikiBase}} <html> <head> </head> <body> <div id="TueContent"> <h3>Ni NA buffer</h3> </div> </body> </html>")
 
(6 intermediate revisions not shown)
Line 9: Line 9:
<div id="TueContent">
<div id="TueContent">
 +
<h1>Protocols</h1>
 +
<h3>Ni NA buffers</h3>
 +
 +
<h4> Lysis buffer (pH = 8)</h4>
 +
<table border="0">
 +
  <colgroup>
 +
    <col width="100">
 +
    <col width="300">
 +
  </colgroup>
 +
 +
  <tr>
 +
    <td style="text-align: center">50 mM</td>
 +
    <td>NaH<sub>2</sub>PO<sub>4</sub></td>
 +
  </tr>
 +
 +
  <tr>
 +
    <td style="text-align: center">300 mM</td>
 +
    <td>NaCl</td>
 +
  </tr>
 +
 +
  <tr>
 +
    <td style="text-align: center">10 mM</td>
 +
    <td>Imidazole</td>
 +
  </tr>
 +
</table>
 +
 +
<p>&nbsp;</p>
 +
 +
<h4> Washing buffer (pH = 8)</h4>
 +
<table border="0">
 +
  <colgroup>
 +
    <col width="100">
 +
    <col width="300">
 +
  </colgroup>
 +
 +
  <tr>
 +
    <td style="text-align: center">50 mM</td>
 +
    <td>NaH<sub>2</sub>PO<sub>4</sub></td>
 +
  </tr>
 +
 +
  <tr>
 +
    <td style="text-align: center">300 mM</td>
 +
    <td>NaCl</td>
 +
  </tr>
 +
 +
  <tr>
 +
    <td style="text-align: center">20 mM</td>
 +
    <td>Imidazole</td>
 +
  </tr>
 +
</table>
 +
 +
<p>&nbsp;</p>
 +
 +
<h4> Elution buffer (pH = 8)</h4>
 +
<table border="0">
 +
  <colgroup>
 +
    <col width="100">
 +
    <col width="300">
 +
  </colgroup>
 +
 +
  <tr>
 +
    <td style="text-align: center">50 mM</td>
 +
    <td>NaH<sub>2</sub>PO<sub>4</sub></td>
 +
  </tr>
 +
 +
  <tr>
 +
    <td style="text-align: center">300 mM</td>
 +
    <td>NaCl</td>
 +
  </tr>
 +
 +
  <tr>
 +
    <td style="text-align: center">250 mM</td>
 +
    <td>Imidazole</td>
 +
  </tr>
 +
</table>
 +
-
<h3>Ni NA buffer</h3>
 
</div>
</div>

Latest revision as of 19:41, 17 October 2014


Protocols

Ni NA buffers

Lysis buffer (pH = 8)

50 mM NaH2PO4
300 mM NaCl
10 mM Imidazole

 

Washing buffer (pH = 8)

50 mM NaH2PO4
300 mM NaCl
20 mM Imidazole

 

Elution buffer (pH = 8)

50 mM NaH2PO4
300 mM NaCl
250 mM Imidazole

Retrieved from "http://2014.igem.org/Team:Tuebingen/Notebook/Protocols/Ni_NTA_Buffers"