Team:Paris Saclay/Notebook/September/16
From 2014.igem.org
(Difference between revisions)
(→Digestion of PS topo plasmids) |
(→PCR) |
||
Line 49: | Line 49: | ||
[https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15#PCR see here] | [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15#PCR see here] | ||
+ | |||
+ | [[File:1609 chromo topo 2-9 jaune 10-17 bleu.jpg|400px] |
Revision as of 12:04, 26 September 2014
Contents |
Lab Work
By Mélanie
D- Lemon scent
Plasmids extraction
Extraction of the plasmid from the culture made yesterday(PS)
Digestion of PS topo plasmids
by PacI and PSTI
(well 2 and 3 : PacI digestion)
Ligation
After digestion, ligation overnight in PPS1
PCR Colony
Test of the transformation made yesterday to know if we had obtained a plasmid]
primer to ckeck the direction of the insert PPS2 : iPS72-96
PPS4 : ips72-97
= no insert (just well 1-9)
liquide culture
liquide culture from yesterday's colony to make stock tomorrow
PCR cad to cloning in pSBIC3
PCR and digestion PSTI/ecoRI and ligation
PCR GS
PCR to check if we have the insert in our topo plasmid
B- Construction of the fusion protein (color)
PCR
PCR to check if we have the insert in our topo plasmid
[[File:1609 chromo topo 2-9 jaune 10-17 bleu.jpg|400px]