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Team:Paris Saclay/Notebook/June/30
From 2014.igem.org
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* MG1655Z1 | * MG1655Z1 | ||
| - | 1mL of an overnight culture in 100mL of LB | + | 1mL of an overnight culture in 100mL of LB. |
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'''Maher & Mathias''' | '''Maher & Mathias''' | ||
| - | We | + | We use the DH5α provided by our advisors for the aforementioned Biobricks. |
Spread on petri dishes with the concentration 10^-1 and 10^-2. | Spread on petri dishes with the concentration 10^-1 and 10^-2. | ||
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* Aliquots of antibiotics : | * Aliquots of antibiotics : | ||
| + | |||
'''Marie & Mathieu''' | '''Marie & Mathieu''' | ||
| - | + | ||
| - | + | Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml. | |
| - | + | (Do the same for Kanamycin and Ampicillin) | |
* Petri dishes | * Petri dishes | ||
| + | |||
'''Romain''' | '''Romain''' | ||
| - | + | ||
| + | Add 250µl of antibiotics in 250ml of LB. Pour in petri dishes. | ||
Revision as of 12:46, 11 July 2014
Contents |
Monday 30th June
Lab work
1 - Culture or the supercompetents:
Mathias
- MG1655
- MG1655Z1
1mL of an overnight culture in 100mL of LB.
2 - Réhydration of selected biobricks:
Juliette
- BBQ_I9026
- BBQ_K592025
- BBQ_K786002
- BBQ_K786003
- BBQ_K778002
- BBQ_K592011 {2 times}
- BBQ_K592009
- BBQ_K1033910 {idem}
- BBQ_J45014
- BBQ_K517003
3 - Transformations :
Maher & Mathias
We use the DH5α provided by our advisors for the aforementioned Biobricks. Spread on petri dishes with the concentration 10^-1 and 10^-2.
4 - Preparations :
- Aliquots of antibiotics :
Marie & Mathieu
Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml. (Do the same for Kanamycin and Ampicillin)
- Petri dishes
Romain
Add 250µl of antibiotics in 250ml of LB. Pour in petri dishes.
People there:
- Instructors : Solenne.
- Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
