Team:Paris Saclay/Notebook/June/30

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(Monday 30th June)
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{{Team:Paris_Saclay/notebook_header}}
=Monday 30th June=
=Monday 30th June=
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==Lab work==
==Lab work==
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=== The odor-free ''E. coli'' chassis===
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====1 - Culture or the supercompetents:====
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====Culture of the supercompetents====
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''by Mathias''
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'''Mathias'''
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* MG1655
* MG1655
* MG1655Z1
* MG1655Z1
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1mL of an overnight culture in 100mL of LB
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1ml of an overnight culture in 100ml of LB.
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====2 - Réhydration of selected biobricks:====
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====Rehydration of selected biobricks====
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''by Juliette''
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'''Juliette'''
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* BBa_I9026 (rhII translational unit)
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* BBa_K592025 (BB0034-amilCP)
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* BBa_K786002 (sensory rhodopsin II & Tar sensitive to blue-green light)
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* BBa_K786003 (sensory rhodopsin I & Tar sensitive to orange light)
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* BBa_K778002
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* BBa_K592011 (cjBlue green chromoprotein)(2 times)
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* BBa_K592009 (amilCP blue chromoprotein)
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* BBa_K103391 (Yellow chromoprotein and Lime yellow-green chromoprotein)(2 times)
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* BBa_J45014 (lemon scent, alcohol acetyltransferase I)
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* BBa_K517003 (lemon scent, β-pinene)
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* BBQ_I9026
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====Transformations====
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* BBQ_K592025
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''by Maher & Mathias''
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* BBQ_K786002
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* BBQ_K786003
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* BBQ_K778002
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* BBQ_K592011 {2 times}
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* BBQ_K592009
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* BBQ_K1033910 {idem}
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* BBQ_J45014
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* BBQ_K517003
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====3 - Transformations :====
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We use the ''E. coli'' DH5α supercompetent cells provided by our advisors for the aforementioned Biobricks.
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Spread on petri dishes with the concentration 10<sup>-1</sup> and 10<sup>-2</sup>.
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'''Maher & Mathias'''
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====Preparations====
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''by Marie and Mathieu''
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We used the DH5α provided by our advisors for the aforementioned Biobricks.
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* Aliquots of antibiotics
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Spread on petri dishes with the concentration 10^-1 and 10^-2.
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====4 - Preparations:====
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Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml.
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(Do the same for Kanamycin and Ampicillin)
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'''Marie, Mathieu & Romain'''
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''by Romain''
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* Aliquots of antibiotics
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* Petri dishes (with antiobiotics)
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* Petri dishes
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Add 250µl of antibiotics in 250ml of LAG. Pour in Petri dishes.
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''by Arnaud''
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* Range of gelose with different concentration of agar
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Add in 6 bottles of 500ml 2g of LB (lysogeny broth), and in each bottle add successively 1500mg (15mg/ml), 2000mg (20mg/ml), 2500mg (25mg/ml), 3000mg (30mg/ml), 3500mg (35mg/ml) and 4000mg (40mg/ml) of agar. And just before autoclaving complete each bottle with 100ml of reverse osmosis-purified water.
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==Photo of the Day==
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[[File:Paris Saclay 30_june.jpg|400px|center]]
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'''People there:'''
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'''Members present:'''
* Instructors : Solenne.
* Instructors : Solenne.
* Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
* Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
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{{Team:Paris_Saclay/notebook_footer}}
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[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
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Latest revision as of 15:46, 14 October 2014

Contents

Monday 30th June

Lab work

The odor-free E. coli chassis

Culture of the supercompetents

by Mathias

  • MG1655
  • MG1655Z1

1ml of an overnight culture in 100ml of LB.

Rehydration of selected biobricks

by Juliette

  • BBa_I9026 (rhII translational unit)
  • BBa_K592025 (BB0034-amilCP)
  • BBa_K786002 (sensory rhodopsin II & Tar sensitive to blue-green light)
  • BBa_K786003 (sensory rhodopsin I & Tar sensitive to orange light)
  • BBa_K778002
  • BBa_K592011 (cjBlue green chromoprotein)(2 times)
  • BBa_K592009 (amilCP blue chromoprotein)
  • BBa_K103391 (Yellow chromoprotein and Lime yellow-green chromoprotein)(2 times)
  • BBa_J45014 (lemon scent, alcohol acetyltransferase I)
  • BBa_K517003 (lemon scent, β-pinene)

Transformations

by Maher & Mathias

We use the E. coli DH5α supercompetent cells provided by our advisors for the aforementioned Biobricks. Spread on petri dishes with the concentration 10-1 and 10-2.

Preparations

by Marie and Mathieu

  • Aliquots of antibiotics

Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml. (Do the same for Kanamycin and Ampicillin)

by Romain

  • Petri dishes (with antiobiotics)

Add 250µl of antibiotics in 250ml of LAG. Pour in Petri dishes.

by Arnaud

  • Range of gelose with different concentration of agar

Add in 6 bottles of 500ml 2g of LB (lysogeny broth), and in each bottle add successively 1500mg (15mg/ml), 2000mg (20mg/ml), 2500mg (25mg/ml), 3000mg (30mg/ml), 3500mg (35mg/ml) and 4000mg (40mg/ml) of agar. And just before autoclaving complete each bottle with 100ml of reverse osmosis-purified water.

Photo of the Day

Paris Saclay 30 june.jpg

Members present:

  • Instructors : Solenne.
  • Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.

Back to the calendar