Team:Paris Saclay/Notebook/July/3

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Contents

Thursday 3rd July

Lab Work

1 - Phages P1 transduction in MG1655 and MG1655Z1

by Mathias

In 2ml Eppendorf :

4 eppendorf MG1655 (0.1ml + 50µl CaCl)
4 eppendorf MG1655Z1 (0.1ml + 50µl CaCl)

Add 100µl of the different phages. Wait 20 minutes!

Add in each eppendorf : 1ml LB liquid + 500µl citrate at 5.10^-3M Wait 1 or 2 hours at 37°C with agitation

Pour in LB Kan petri dishes, incubate at 37°C. (Turn the petri dishes over when there is no more liquid in!)

2 - Plasmid DNA Purification

by Fabio, Maher, Mathieu, Marie and Romain

  • K773002 (x2)
  • K517003 (x2)
  • K773002 (x2)
  • K592011 (x2)
  • K1033910 (x2)
  • K592009 (x2)
  • K786002 (x2)
  • K786003 (x2)
  • J45014 (x2)
  • I9026 (x2)

Protocol


People there:

Instructors : Alice & Solenne. Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.


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