Team:Paris Saclay/Notebook/July/3

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Thursday 3rd July

Lab Work

1 - Phages P1 transduction in MG1655Z1

Mathieu

In 2ml Eppendorf :

  *4 eppendorf MG1655 (0.1ml + 50µl CaCl)
  *4 eppendorf MG1655Z1 (0.1ml + 50µl CaCl)

Add 100µl of the different phages. Wait 20 minutes!

Add in each eppendorf :

  *1ml LB liquid
  *500µl citrate at 5.10^-3M

Wait 1 or 2 hours at 37°C with agitation


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