"
Team:Paris Saclay/Notebook/July/3
From 2014.igem.org
(Difference between revisions)
(→Thursday 3rd July) |
(→Thursday 3rd July) |
||
| Line 16: | Line 16: | ||
Add in each eppendorf : 1ml LB liquid + 500µl citrate at 5.10^-3M | Add in each eppendorf : 1ml LB liquid + 500µl citrate at 5.10^-3M | ||
'''Wait 1 or 2 hours at 37°C with agitation''' | '''Wait 1 or 2 hours at 37°C with agitation''' | ||
| + | |||
| + | Pour in LB Kan petri dishes, incubate at 37°C.(''Turn the petri dishes over when there is no more liquid in'') | ||
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | [https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | ||
Revision as of 14:08, 11 July 2014
Thursday 3rd July
Lab Work
1 - Phages P1 transduction in MG1655Z1
Mathieu
In 2ml Eppendorf :
- 4 eppendorf MG1655 (0.1ml + 50µl CaCl)
- 4 eppendorf MG1655Z1 (0.1ml + 50µl CaCl)
Add 100µl of the different phages. Wait 20 minutes!
Add in each eppendorf : 1ml LB liquid + 500µl citrate at 5.10^-3M
Wait 1 or 2 hours at 37°C with agitation
Pour in LB Kan petri dishes, incubate at 37°C.(Turn the petri dishes over when there is no more liquid in)
