Team:Paris Saclay/Notebook/July/3

From 2014.igem.org

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{{Team:Paris_Saclay/notebook_header}}
=Thursday 3rd July=
=Thursday 3rd July=
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==Lab Work==
==Lab Work==
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===- The chassis coli Odor free===
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===1 - Phages P1 transduction in MG1655 and MG1655Z1===
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====Phages P1 transduction in MG1655 and MG1655Z1====
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''by Mathias''
''by Mathias''
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Pour in LB Kan petri dishes, incubate at 37°C. (''Turn the petri dishes over when there is no more liquid in!'')
Pour in LB Kan petri dishes, incubate at 37°C. (''Turn the petri dishes over when there is no more liquid in!'')
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===2 - Plasmid DNA Purification===
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===Plasmid DNA Purification===
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''by Fabio, Maher, Mathieu, Marie and Romain''
''by Fabio, Maher, Mathieu, Marie and Romain''
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol]
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==Photo of the Day==
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[[File:Paris Saclay 3_july.jpg|600px|center]]
'''Members present:'''
'''Members present:'''
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*Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
*Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
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[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
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{{Team:Paris_Saclay/notebook_footer}}

Latest revision as of 15:34, 14 October 2014

Contents

Thursday 3rd July

Lab Work

- The chassis coli Odor free

Phages P1 transduction in MG1655 and MG1655Z1

by Mathias

In 2ml Eppendorf :

4 eppendorf MG1655 (0.1ml + 50µl CaCl)
4 eppendorf MG1655Z1 (0.1ml + 50µl CaCl)

Add 100µl of the different phages. Wait 20 minutes!

Add in each eppendorf : 1ml LB liquid + 500µl citrate at 5.10^-3M Wait 1 or 2 hours at 37°C with agitation

Pour in LB Kan petri dishes, incubate at 37°C. (Turn the petri dishes over when there is no more liquid in!)

Plasmid DNA Purification

by Fabio, Maher, Mathieu, Marie and Romain

  • BBa_K773002 (x2)
  • BBa_K517003 (x2)
  • BBa_K773002 (x2)
  • BBa_K592011 (x2)
  • BBa_K1033910 (x2)
  • BBa_K592009 (x2)
  • BBa_K786002 (x2)
  • BBa_K786003 (x2)
  • BBa_J45014 (x2)
  • BBa_I9026 (x2)

Protocol


Photo of the Day

Paris Saclay 3 july.jpg

Members present:

  • Instructors : Alice & Solenne.
  • Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.

Back to the calendar