Team:Paris Saclay/Notebook/July/22

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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
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===3 - Bacterial Culture===
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''by Fabio and Marie''
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One liquid culture with 5ml of LB (37°C - 150 rpm), IL1403 from the concentrated colony.
'''Members there''':
'''Members there''':

Revision as of 14:53, 22 July 2014

Contents

Tuesday 22st July

Lab Work

1 - Samples for stock

by Fabio

We collected 1ml of each sample, added 240µl of glycerol (87%) and stored at -80°C.

  • K1033902
  • K1033905
  • K1033910
  • K1033913
  • K1033922
  • K1033925
  • K1033927
  • K1033929
  • K103921
  • K45017
  • K731201
  • K762100

from Liquide Bacterial Cultures transformed the 21st July

2 - Electrophoresis

by Arnaud (Process A), Fabio (gel), Marie and Romain (Process B)

We used 2µL of DNA of the following Biobricks in a 1% Agarose Gel.

Process A

Process B

Results:

  • A:
  • B:
  • C:

Protocol

3 - Bacterial Culture

by Fabio and Marie

One liquid culture with 5ml of LB (37°C - 150 rpm), IL1403 from the concentrated colony.

Members there:

  • Instructors and advisors: Solenne.
  • Students: Arnaud, Fabio, Juliette, Mathieu, Marie, Pierre, Romain, Sean and Terry.

Back to the calendar

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