Team:Paris Saclay/Notebook/July/17

From 2014.igem.org

(Difference between revisions)
(Created page with "=Thursday 17th July= ==Lab work== [https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]")
m
 
(19 intermediate revisions not shown)
Line 1: Line 1:
 +
{{Team:Paris_Saclay/notebook_header}}
=Thursday 17th July=
=Thursday 17th July=
-
 
==Lab work==
==Lab work==
 +
===The chassis coli Odor free===
 +
====1 - Preparation of supercompetent cells====
 +
''By Mathias & Romain''
 +
 +
Strains used: MG1655 (x2), MG1655Z1 (x2).
 +
 +
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
 +
 +
====2 - Bacterial Culture====
 +
''by Fabio and Maher''
 +
 +
2 liquid cultures with 5ml of LB and 5µl of amp (at 9am - 37°C - 150 rpm), both BBa_J23119 from the concentrated colony made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 16th July]
 +
 +
====3 - PCR and Electrophoresis====
 +
[[File:Electrophoresis_17.07.2014.jpg|400px|right]]
 +
''by Arnaud & Mathias''
 +
 +
PCR targeting with oligos IPS70 and IPS71 of pOSV230 (Apramycine).
 +
 +
#Molecular weight marker 1kb
 +
#Empty
 +
#IPS70 and IPS71 (1)
 +
#IPS70 and IPS71 (2)
 +
 +
'''Results:'''
 +
 +
Failure, no PCR products.
 +
 +
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
 +
 +
==Modeling==
 +
====Study of bacterial population growth in space and time====
 +
[[File:Book.jpg|100px|left]]
 +
''by Pierre & Juliette''
 +
 +
We have finished to study a first model for our problem. It is a simple birth/death model and we do a mix of deterministic and stochastic model in order to be most precise as possible.
 +
 +
We have focused our study on this (excellent !) book :  ''Modeling Biological Populations in Space and Time''
 +
by '''Eric Renshaw''', and especially on chapters 1, 2 and 3.
 +
 +
We now hope to succeed improving our model to be more realistic !
 +
 +
 +
==Photo of the Day==
 +
[[File:Paris Saclay 17_july.jpg|600px|center]]
-
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
+
'''Members present:'''
 +
* Instructors and advisors: Alice and Solenne.
 +
* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
 +
{{Team:Paris_Saclay/notebook_footer}}

Latest revision as of 13:57, 17 October 2014

Contents

Thursday 17th July

Lab work

The chassis coli Odor free

1 - Preparation of supercompetent cells

By Mathias & Romain

Strains used: MG1655 (x2), MG1655Z1 (x2).

Protocol

2 - Bacterial Culture

by Fabio and Maher

2 liquid cultures with 5ml of LB and 5µl of amp (at 9am - 37°C - 150 rpm), both BBa_J23119 from the concentrated colony made the 16th July

3 - PCR and Electrophoresis

Electrophoresis 17.07.2014.jpg

by Arnaud & Mathias

PCR targeting with oligos IPS70 and IPS71 of pOSV230 (Apramycine).

  1. Molecular weight marker 1kb
  2. Empty
  3. IPS70 and IPS71 (1)
  4. IPS70 and IPS71 (2)

Results:

Failure, no PCR products.

Protocol

Modeling

Study of bacterial population growth in space and time

Book.jpg

by Pierre & Juliette

We have finished to study a first model for our problem. It is a simple birth/death model and we do a mix of deterministic and stochastic model in order to be most precise as possible.

We have focused our study on this (excellent !) book : Modeling Biological Populations in Space and Time by Eric Renshaw, and especially on chapters 1, 2 and 3.

We now hope to succeed improving our model to be more realistic !


Photo of the Day

Paris Saclay 17 july.jpg

Members present:

  • Instructors and advisors: Alice and Solenne.
  • Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

Back to the calendar