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Team:Paris Saclay/Notebook/July/11
From 2014.igem.org
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*MG1655Z1 | *MG1655Z1 | ||
*DH5α+ (given by Solenne) | *DH5α+ (given by Solenne) | ||
| + | (The strains DH5α, MG1655 and MG1655Z1 were prepared by ''Mathias & Mathieu'') | ||
BioBricks used: | BioBricks used: | ||
Revision as of 14:02, 12 July 2014
Contents |
Friday 11th July
Lab work
1 - Fill in the WIKI (Notebook and Protocols)
Juliette & Romain
2 - Transformation of competent cells E.coli
Fabio & Maher
Competent strains used:
- DH5α
- MG1655
- MG1655Z1
- DH5α+ (given by Solenne)
(The strains DH5α, MG1655 and MG1655Z1 were prepared by Mathias & Mathieu)
BioBricks used:
- J23100 (Amp R)(Dilutions: Concentrated, 0 and 10^-1)
- J23106 (Amp R)(Dilutions: Concentrated, 0 and 10^-1)
- J23114 (Amp R)(Dilutions: Concentrated, 0 and 10^-1)
+ pET28a (Kan R)(Dilutions: 0, 10^-1 and 10^-2) (pET28a is a plasmid used to verify that the 4 strains are competent).
The negative controles:
- pET28a: without plasmid, nothing grows.
- J23100, J23106, J23114: without the BioBrick, nothing brows.
Results:
- pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well.
- J23100, J23106, J23114: The strain DH5α+ grow well and the 3 others strains grow less. They have to be more concentrated.
