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Team:Paris Saclay/Notebook/July/10
From 2014.igem.org
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(→2 - Transformation of competent cells E.coli) |
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BioBricks used: | BioBricks used: | ||
| - | * | + | *BBa_J23100 (Amp R)(Dilutions: Concentrated, 0 and 10<sup>-1</sup>) |
| - | * | + | *BBa_J23106 (Amp R)(Dilutions: Concentrated, 0 and 10<sup>-1</sup>) |
| - | * | + | *BBa_J23114 (Amp R)(Dilutions: Concentrated, 0 and 10<sup>-1</sup>) |
+ pET28a (Kan R)(Dilutions: 0, 10<sup>-1</sup> and 10<sup>-2</sup>) | + pET28a (Kan R)(Dilutions: 0, 10<sup>-1</sup> and 10<sup>-2</sup>) | ||
(pET28a is a plasmid used to verify that the 4 strains are competent). | (pET28a is a plasmid used to verify that the 4 strains are competent). | ||
Revision as of 15:34, 23 July 2014
Contents |
Thursday 10th July
Lab work
1 - Plasmid DNA Extraction:
by Romain and Sean
Plasmids used:
- BBa_B0015, dble ter
- FNR_RBS_amilCP-Ter DH5αZ1
2 - Transformation of competent cells E.coli
by Fabio and Maher
Competent strains used:
- DH5α
- MG1655
- MG1655Z1
- DH5α+ (given by Solenne)
(The strains DH5α, MG1655 and MG1655Z1 were prepared by Mathias and Mathieu)
BioBricks used:
- BBa_J23100 (Amp R)(Dilutions: Concentrated, 0 and 10-1)
- BBa_J23106 (Amp R)(Dilutions: Concentrated, 0 and 10-1)
- BBa_J23114 (Amp R)(Dilutions: Concentrated, 0 and 10-1)
+ pET28a (Kan R)(Dilutions: 0, 10-1 and 10-2) (pET28a is a plasmid used to verify that the 4 strains are competent).
Negative control groups:
- pET28a: without plasmid, nothing grows.
- J23100, J23106, J23114: without the BioBrick, nothing grows.
Results:
- pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well.
- J23100, J23106, J23114: The strain DH5α+ grow well and the 3 others strains grow less. They have to be more concentrated.
