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Team:Paris Saclay/Notebook/August/4
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The culture has been placed at 42°C for 17 min then cooled 10 min before centrifugation. | The culture has been placed at 42°C for 17 min then cooled 10 min before centrifugation. | ||
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| + | '''Members there''': | ||
| + | * Instructors and advisors: Alice, Solenne and Sylvie. | ||
| + | * Students: Eugene, Fabio, Hoang Vu, Juliette, Pierre, Romain, Sean and Terry. | ||
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | [https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | ||
Revision as of 10:17, 4 August 2014
Contents |
Monday 4th August
Lab Work
A - The frame coli Odor free
Striate on Dishes
by Juliette & Romain
We striated MG1655 and MG1655Z1 on LB and Kan dishes, from the striates prepared on LB dishes 1st August
D - Lemon scent
Gel electrophoresis of BBa_K762100
by Sean
Samples used were prepared on the 30th July.
Results
From left to right: 5 µl of BBa_K762100 + "old" Phusion enzyme, 5 µl of BBa_K762100 + "new" Phusion enzyme
Electroporation
by Terry
Strain used: DY330 containing plasmid pJBEI6409.
We had to switch the Limonen Synthase cassette with the Apramycin resistance.
The culture has been placed at 42°C for 17 min then cooled 10 min before centrifugation.
Members there:
- Instructors and advisors: Alice, Solenne and Sylvie.
- Students: Eugene, Fabio, Hoang Vu, Juliette, Pierre, Romain, Sean and Terry.
