Team:Paris Saclay/Notebook/August/14

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Contents

Thursday 14th August

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Lab Work

by Melanie

Preparation of DH5a CaCl2 Competent cell

Protocol

D-Lemon Scent

by Melanie

From the 20 ml of odor free culture (pJBEI Apra) done yesterday (by Laetitia)

Plasmide extraction without Nucleospin


Transformation of DH5 alpha

We prepare a LB agar environment with a concentration of:

  • 0.5 mM of IPTG
  • 0.008% of Xgal
  • 1/2000 of Ampicilline

and we spread our preparation in a petri dish and incubate overnight at 37°C.


Protocol