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Team:Evry/Overview/Achievements/Projects
From 2014.igem.org
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<FONT color=#003399><h5><b><img src="https://static.igem.org/mediawiki/2014/6/61/Cells_pic.png" width="5%"/>Chassis characterization</b></h5> </font> | <FONT color=#003399><h5><b><img src="https://static.igem.org/mediawiki/2014/6/61/Cells_pic.png" width="5%"/>Chassis characterization</b></h5> </font> | ||
<p><ul style="list-style:none;"> | <p><ul style="list-style:none;"> | ||
| - | <br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/GenomeAssembly"> Genome Sequencing </a | + | <br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/GenomeAssembly"> Genome Sequencing </a>of <i> Pseudovibrio denitrificans to collect informations defense mechanisms, transposons putative insertion localisation and to possess a assembled genome for RNAseq.</i> </li> |
<br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Chassis">Growth conditions optimization</a> </li> | <br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Chassis">Growth conditions optimization</a> </li> | ||
<br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Chassis"> Antibiotic sensitivity</a> tests shown a potential resistance to tetracycline and ampicilline</li> | <br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Chassis"> Antibiotic sensitivity</a> tests shown a potential resistance to tetracycline and ampicilline</li> | ||
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<FONT color=#003399><h5><b><img src="https://static.igem.org/mediawiki/2014/6/6e/Plasmid_khd.png" width="5%"/>New Vector</b></h5> </font> | <FONT color=#003399><h5><b><img src="https://static.igem.org/mediawiki/2014/6/6e/Plasmid_khd.png" width="5%"/>New Vector</b></h5> </font> | ||
<p><ul style="list-style:none;"> | <p><ul style="list-style:none;"> | ||
| - | <br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Transposons">Transposon-based vector</a> </li> | + | <br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Transposons">Transposon-based vector</a> was created to overcome resistance mechanisms to DNA entrance</li> |
<br> <li>- Proof of concept to use it in <i>Pseudovibrio denitrificans</i> </li> | <br> <li>- Proof of concept to use it in <i>Pseudovibrio denitrificans</i> </li> | ||
</ul> | </ul> | ||
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<p><ul style="list-style:none;"> | <p><ul style="list-style:none;"> | ||
<br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Sensors"> New PCB sensor based on bphR1 promoter and binding protein bphR2. </a> </li> | <br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Sensors"> New PCB sensor based on bphR1 promoter and binding protein bphR2. </a> </li> | ||
| - | <br> <li>- An optimized version of <a href="https://2014.igem.org/Team:Evry/Biology/Sensors"> the Phenol sensors </a></li> | + | <br> <li>- An optimized version of <a href="https://2014.igem.org/Team:Evry/Biology/Sensors"> the Phenol sensors from Pekin iGEM 2013 team </a></li> |
| - | <br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/RNAseq"> RNA-seq data </a> | + | <br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/RNAseq"> RNA-seq data </a> shall be used to find new promoters</li> |
</ul> | </ul> | ||
</p> | </p> | ||
Revision as of 03:51, 18 October 2014
Projects Achievements
Chassis characterization
- - Genome Sequencing of Pseudovibrio denitrificans to collect informations defense mechanisms, transposons putative insertion localisation and to possess a assembled genome for RNAseq.
- - Growth conditions optimization
- - Antibiotic sensitivity tests shown a potential resistance to tetracycline and ampicilline
- - Electroporation Transformation protocol establishment that never been done before
New Vector
- - Transposon-based vector was created to overcome resistance mechanisms to DNA entrance
- - Proof of concept to use it in Pseudovibrio denitrificans
New or Optimized sensors Sensors
- - New PCB sensor based on bphR1 promoter and binding protein bphR2.
- - An optimized version of the Phenol sensors from Pekin iGEM 2013 team
- - RNA-seq data shall be used to find new promoters
