Team:BNU-China

Nitrogen fixing ability of azobobacter is significant for the growth of grass family. The stronger of its ability, the less chemical fertilizer will be used. Molybdenum is a key integral part of nitrogen fixing enzyme ModA in azobobacter and Mo will is also important for the growth of plants themselves. However, Mo is a kind of heavy mental as well and the abuse of it will cause environment pollution. Hence, spot application of Mo is of great importance. A plasmid was transfer into E.coli Promethus to make it express fusion protein, one side of which anchors on the out membrane and the other side of which catches molybdate. The engineered E.coli Promethus can bind molybdate and with the help of auxin tendency system design by Imperial College 2013, our Promethus will head for the roots. A suicide system depending on time is also design by us for the reason of bio-safety. E.coli Proethus will deliver Mo directly to the root of plants, so the heavy mental pollution of Mo will almost decrease to Zero according to our experiment results. This will also open a new chapter of biological fertilizer.