Team:Paris Saclay/Notebook/August/4

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Contents

Monday 4th August

Lab Work

A - The chassis coli Odor free

Striate on Dishes

by Juliette & Romain

We striated MG1655 and MG1655Z1 on LB and Kan dishes, from the striates prepared on LB dishes 1st August

C - Salicylate Inducible Suppressing System

Plasmid DNA Purification

by Eugene and Fabio

  • Ligation of BBa_J61051 and BBa_K228001 made the 30th July

From Liquid Culture made the 1st August

Digestion to check

In progress

Electrophoresis

In progress

D - Lemon scent

Gel electrophoresis of BBa_K762100

by Sean

Samples used were prepared on the 30th July.

Results

From left to right: 5 µl of BBa_K762100 + "old" Phusion enzyme, 5 µl of BBa_K762100 + "new" Phusion enzyme

Electroporation

by Terry

Strain used: DY330 containing plasmid pJBEI6409.

We had to switch the Limonen Synthase cassette with the Apramycin resistance.

The culture has been placed at 42°C for 17 min then cooled 10 min before centrifugation.

Polymerase chain reaction

by Sean & Pierre

BBa_K517003

component volume
H2O 35.5μl
Phusion buffer 5X 10μl
dNTPs 2μl
iPS68bis 1μl
iPS69 1μl
DNA 1μl
Phusion enzyme 0.5μl


BBa_K762100

For this BioBrick two tubes were prepared: one with undiluted DNA and one with DNA diluted 10-1.

component volume
H2O 35.5μl
Phusion buffer 5X 10μl
dNTPs 2μl
iPS66 1μl
iPS67 1μl
DNA 1μl
Phusion enzyme 0.5μl

Members there:

  • Instructors and advisors: Alice, Solenne and Sylvie.
  • Students: Eugene, Fabio, Hoang Vu, Juliette, Pierre, Romain, Sean and Terry.

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