Team:Heidelberg/AwesomeSheet/Purifications

From 2014.igem.org

Revision as of 15:25, 17 October 2014 by Jan glx (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

– Purifications

ABCDEFGHLMNPQRSTABADAFAGAHAIAJ
1
präID
2
IDMidnightDoc-IDDateTimeEperimentatorSourceMethodContentSolventVcleftRelated StockComment2beUsedOriginRelated sequenzing
3
TypIDTypeID
Plasmid-ID
Nameµl
ng/µl
V/µLBarcodeResult
4
O1O106-0815:30Jakob, JanGrowth37C2
Qiagen miniprep kit
Plasmid11BBa_J61002-BBa_J2311050.024248.011
lysis was performed in the centrifuge tube leading to a potentially huge loss of DNA
D2014-4-19D
BF8244ok
5
O2O206-0815:30Jakob, JanGrowth38C2
Qiagen miniprep kit
Plasmid22BBa_J61002-BBa_J2311850.041945.612
lysis was performed in the centrifuge tube leading to a potentially huge loss of DNA
D2014-4-21B
BF8245ok
6
O3O306-0815:30Jakob, JanGrowth39C2
Qiagen miniprep kit
Plasmid33BBa_J61002-BBa_J2310050.023943.813
lysis was performed in the centrifuge tube leading to a potentially huge loss of DNA
D2014-4-17D
BF8246ok
7
O4O406-0723:00
Silvan, Jakob and Max W.
Growth40C1
Qiagen miniprep kit
Plasmid44pSB1C3-BBa_C001250.018540.610was accidantially switchedD2014-2-2NBF8250was prevoiously thought to be BBa_I15016
8
O5O506-0815:30Jakob, JanGrowth41C1
Qiagen miniprep kit
Plasmid55pSB1C3-BBa_E024050.024836.914
lysis was performed in the centrifuge tube leading to a potentially huge loss of DNA
D2014-2-24B
BF8248insertion before prefix leading to additional EcoRI site
9
O6O606-0723:00
Silvan, Jakob and Max W.
Growth42C1
Qiagen miniprep kit
Plasmid66pSB1C3-BBa_R001050.011937.68D2014-3-4GBF8249ok
10
O7O706-0723:00
Silvan, Jakob and Max W.
Growth43C1
Qiagen miniprep kit
Plasmid77pSB1C3-BBa_I1501650.014541.17was accidentially switchedD2014-3-17EBF8247was previously thought to be BBa_C0012
11
O8O806-0815:30Jakob, JanGrowth44C1
Qiagen miniprep kit
Plasmid88pSB1C3-BBa_I1501750.019337.615
lysis was performed in the centrifuge tube leading to a potentially huge loss of DNA
D2014-3-17G
BF8251ok
12
O12O1206-0723:00
Silvan, Jakob and Max W.
Growth10C1
Qiagen miniprep kit
Plasmid1212pUC1950.021648.09O12-2
13
O12-2O12
s/vSuyTkAuEqcHYkHK6
06-04ExternInvitrogenPlasmid1212pUC1950.0028.0
14
O13O1306-1216:00JakobGrowth10
Qiagen miniprep kit
Plasmid1313pSB1C3-pKH6insert50.010247.016BF8252, BF8253ok, ok
15
O14O1406-1216:00JakobGrowth11
Qiagen miniprep kit
Plasmid1414pSB1C3-pKH7insert50.09548.017BF8253, BF8254ok, ok
16
O15O1506-1216:00JakobGrowth12
Qiagen miniprep kit
Plasmid1515pSB1C3-pKH18insert50.012548.018BF8254, BF8255negativ, araC, negativ, Shewanella violacea
17
O12-3O1206-1815:00JakobPurificationO12DilutionPlasmid1212pUC191,000.011,000.0
18
O12-4O1206-1815:00JakobPurificationO12-3DilutionPlasmid1212pUC191,000.001,000.0
19
O16O1606-220:40SilvanGrowth52C1
Qiagen miniprep kit
Plasmid1616pGBMCS-SortA50.05941.619O12-2II2247ok
20
O17O1706-220:40SilvanGrowth53C1
Qiagen miniprep kit
Plasmid1717pHYRSF150.032746.220O12-2II2232ok
21
O18O1806-220:40SilvanGrowth54C1
Qiagen miniprep kit
Plasmid1818pHYRSF1-0250.026345.121O12-2II2233ok
22
O19O1906-220:40SilvanGrowth55C1
Qiagen miniprep kit
Plasmid1919pJJDuet3050.029749.222O12-2II2234Primer hat an 2 Stellen gebunden, sieht aber ok aus
23
O20O2006-220:40SilvanGrowth56C1
Qiagen miniprep kit
Plasmid2020pSFBAD0950.06045.823
D2014-4-19D
II2235ok
24
O22O2206-2512:00
Maggie, Silvan
Growth57C1
Qiagen miniprep kit
Plasmid2222pFastBac-mDNMT150.023448.924O22-0
25
O23O2306-2512:00
Maggie, Silvan
Growth58C1
Qiagen miniprep kit
Plasmid2323pRSFDuet-1-mDNMT1(731-1602)50.019144.825O23-0
II2225, II2227, II2228, II2229
ok, ok, ok, ok
26
O24O2406-2512:00
Maggie, Silvan
Growth59C1
Qiagen miniprep kit
Plasmid2424pIT7750.05245.226O24-0II2236ok
27
O25O2506-2512:00
Maggie, Silvan
Growth60C1
Qiagen miniprep kit
Plasmid2525pIT2950.07846.827O25-0II2237ok
28
O26O2606-2512:00
Maggie, Silvan
Growth61C1
Qiagen miniprep kit
Plasmid2626pIT2150.06246.028O26-0II2238ok
29
O27O2706-2512:00
Maggie, Silvan
Growth62C1
Qiagen miniprep kit
Plasmid2727pCL2050.04143.829O27-0II2239ok
30
O28O2806-2512:00
Maggie, Silvan
Growth63C1
Qiagen miniprep kit
Plasmid2828pAU0750.07143.030O28-0II2240ok
31
O29O2906-2512:00
Maggie, Silvan
Growth64C1
Qiagen miniprep kit
Plasmid2929pVS4150.0115031O29-0II2241ok
32
O30O3006-2512:00
Maggie, Silvan
Growth65C1
Qiagen miniprep kit
Plasmid3030pVS0750.015246.732O30-0II2242ok
33
O31O31-06-261:00SilvanGrowth66C1
Qiagen miniprep kit
Plasmid3131pRARE750.033
D2014-4-21B
II2224ok, aber falsche Richtung
34
O5-2O506-2813:00
Constantin
PurificationO5DilutionPlasmid55pSB1C3-BBa_E0240248.01239.0
35
O18-2O1806-2813:00
Constantin
PurificationO18DilutionPlasmid1818pHYRSF1-02102.0199.0
36
O13-2O1306-2813:00
Constantin
PurificationO13DilutionPlasmid1313pSB1C3-pKH6insert326.010discarded
37
O17-2O1706-2813:00
Constantin
PurificationO17DilutionPlasmid1717pHYRSF1262.01260.0
38
O19-2O1906-3016:10JanPurificationO19DilutionPlasmid1919pJJDuet30297.01296.0
39
O20-2O2006-3016:15MaxPurificationO20DilutionPlasmid2020pSFBAD0959.5156.5
40
P4P407-0117:00
Charlotte, Constantin
Gel7-2Gel extractionPP4pSB1C3_NpuDnaE-N_FLAG-NpuDnaE-C50.01450.0
41
P1P107-0117:00
Charlotte, Constantin
Gel7-3Gel extractionPP1NpuDnaE-C_pSB1C3-FLAG_6His-BsaI-ccdB50.0950.0
42
P2P207-0117:00
Charlotte, Constantin
Gel7-4Gel extractionPP2ccdB_NpuDnaE-C-6His-BsaI_BasI-NpuDnaE-N50.04050.0
43
P3P307-0117:00
Charlotte, Constantin
Gel7-5Gel extractionPP3NpuDnaE-N_BsaI-ccdB_pSB1C350.01250.0
44
P5P507-0117:00
Charlotte, Constantin
Gel7-6Gel extractionPP5NpuDnaE-C_pSB1C3-FLAG_BsaI-ccdB50.02050.0
45
P7P707-0117:00
Charlotte, Constantin
Gel7-7Gel extractionPP7pSB1C3_SspDnaE-N-taataat_Flag-SspDnaE_C50.04550.0
46
P9P907-0117:00
Charlotte, Constantin
Gel7-8Gel extractionPP9ccdB_SspDnaE-C-BsaI_BsaI-SspDnaE-N50.06550.0
47
P10P1007-0117:00
Charlotte, Constantin
Gel7-9Gel extractionPP10SspDnaE-N_ccdB-BsaI_taataat-pSB1C350.06450.0
48
P12P1207-0117:00
Charlotte, Constantin
Gel7-10Gel extractionPP12ccdB_SspDnaE-C-6His-BsaI_BsaI-SspDnaE-N50.05250.0
49
P8P807-0117:00
Charlotte, Constantin
Gel7-11Gel extractionPP8SspDnaE-C_pSB1C3-Flag_BsaI-ccdB50.02750.0
50
P6P607-0117:00
Charlotte, Constantin
Gel7-12Gel extractionPP6ccdB_NpuDnaE-C-BsaI _BasI-NpuDnaE-N50.05350.0
51
P11P1107-0117:00
Charlotte, Constantin
Gel7-13Gel extractionPP11SspDnaE-C_pSB1C3-Flag_6His-BsaI-ccdB50.03150.0
52
O36O3607-0323:00LisaGrowth130C1
Qiagen miniprep kit
Plasmid3636pSB1AK8-BBa_I71207450.058050D2013-5-6NII2226RFC23 (missing the final G in the prefix and the primary T in the suffix)
53
O35O3507-0323:00LisaGrowth129C2
Qiagen miniprep kit
Plasmid3535pSB1C3-2ZSspH650.0660discardedA35
54
O35-2O3507-0323:00LisaGrowth129C3
Qiagen miniprep kit
Plasmid3535pSB1C3-2ZSspH650.010629.147A35II2230, II2243ok, ok
55
O35-3O3507-0323:00LisaGrowth129C4
Qiagen miniprep kit
Plasmid3535pSB1C3-2ZSspH650.01060discardedA35II2231, II2244ok, ok
56
O35-4O3507-0323:00LisaGrowth129C5
Qiagen miniprep kit
Plasmid3535pSB1C3-2ZSspH650.0560discardedA35
57
O35-5O3507-0323:00LisaGrowth129C6
Qiagen miniprep kit
Plasmid3535pSB1C3-2ZSspH650.01170discardedA35
58
O16-2O1607-0412:45SilvanGrowth52C2
Qiagen miniprep kit
Plasmid1616pGBMCS-SortA50.07750.0O12-2
59
O23-2O2307-0412:45SilvanGrowth131C1
Qiagen miniprep kit
Plasmid2323pRSFDuet-1-mDNMT1(731-1602)50.051447.040discardedO23
60
O38O3807-0623:19Max H.Growth153C1
Qiagen miniprep kit
Plasmid3838pSB1AK8-BBa_I712074-BBa_I1501650.03850.034S38
61
O39O3907-0623:19Max H.Growth154C1
Qiagen miniprep kit
Plasmid3939pSB1AK8-BBa_I712074-BBa_I1501750.05850.035S39
62
O40O4007-0623:19Max H.Growth155C1
Qiagen miniprep kit
Plasmid4040pSB1AK8-BBa_I712074-BBa_E024050.05150.036S40
63
O41O4107-0623:19Max H.Growth156C2
Qiagen miniprep kit
Plasmid4141pSB1AK8-BBa_I712074-S-BBa_J7039050.06850.037S41
64
O37O3707-0623:19Max H.Growth142C1
Qiagen miniprep kit
Plasmid3737pSB1A2-BBa_S0352050.017450.038D2013-5-7J
65
P18P1807-0623:20JanPCR36
PCR purification kit
PP18BBa_E0040_EcoR1_Spe130.0383.3
66
O32O3207-0722:00CarolinGrowth157C1
Qiagen miniprep kit
Plasmid3232pSB1C3-2ZNpuH650.0780discardedA32#2
67
O32-2O3207-0722:00CarolinGrowth157C2
Qiagen miniprep kit
Plasmid3232pSB1C3-2ZNpuH650.0124039discardedA32#2II2245, II2246
insert in the prefix, single insertion in the ccdb part single deletion in the N Intein part, insert in the prefix, single insertion in the ccdb part single deletion in the N Intein part
68
O32-3O3207-0722:00CarolinGrowth157C3
Qiagen miniprep kit
Plasmid3232pSB1C3-2ZNpuH650.0660discardedA32#2
69
O33O3307-0722:00CarolinGrowth158C1
Qiagen miniprep kit
Plasmid3333pSB1C3-2ZNpu50.09639.642use as O32A33II2248, II2249insertion in the prefix, additional His after Flag, bad sequencing
70
O33-2O3307-0722:00CarolinGrowth158C2
Qiagen miniprep kit
Plasmid3333pSB1C3-2ZNpu50.06634.742bdiscardedA33II2252additional His after flag, insertion in the prefix
71
O33-3O3307-0722:00CarolinGrowth158C3
Qiagen miniprep kit
Plasmid3333pSB1C3-2ZNpu50.07135.942cdiscardedA33II2253additional His after flag, insertion in the prefix
72
R18R1807-0518:00JanGel13-2Gel extraction
Restriction
18pSB1C3-BBa_E0240 (X/P)20.01415.7
73
R19R1907-0518:00JanGel13-3Gel extraction
Restriction
19pSB1C3-BBa_I15016 (X/P)20.0915.7
74
R20R2007-0518:00JanGel13-4Gel extraction
Restriction
20pSB1C3-BBa_I15017 (X/P)20.01415.7O2
75
R21R2107-0518:00JanGel13-5Gel extraction
Restriction
21BBa_J61002-BBa_J23100 (S/P)20.01815.7
76
R38R3807-0518:00JanRestriction38
PCR purification kit
Restriction
38pSB1AK8-BBa_I712074 (S/P)20.01213.1
77
O43O4307-0817:13SilvanGrowth164C1
Qiagen miniprep kit
Plasmid4343pSB1C3-BBa_I746907H2O50.03923.446D2014-3-12III2250, II2251,
78
O29,30,31P07-0817:13SilvanGrowth161C1
Qiagen miniprep kit
PlasmidsH2O50.05850.0O30
79
O29,30,31-2
P07-0817:13SilvanGrowth161C2
Qiagen miniprep kit
PlasmidsH2O50.09150.0O30
80
O29,30,31-3
P07-0817:13SilvanGrowth162C1
Qiagen miniprep kit
PlasmidsH2O50.011650.045O29
81
O29,30,31-4
P07-0817:13SilvanGrowth162C2
Qiagen miniprep kit
PlasmidsH2O50.011250.0O29
82
O42O4207-0915:00SilvanGrowth165C1
Qiagen miniprep kit
Plasmid4242pSB1A2-BBa_I71900550.020545.1
D2013-5-15N
83
R44R4407-0821:00CharlotteGel18-3Gel extraction
Restriction
44pSB1C3-2ZSspH6 (BsaI)-6.0
84
R45R4507-0821:00CharlotteGel18-2Gel extraction
Restriction
45pSB1C3-2ZNpuH6 (BsaI)-6.0
85
R46R4607-0821:00CharlotteGel18-4Gel extraction
Restriction
46BBa_E0040_EcoR1_Spe1 (E/S)-4.0
86
R44-2R4407-0921:00CharlotteRestriction44
PCR purification kit
Restriction
44pSB1C3-2ZSspH6 (BsaI)H2O#N/A#N/A#N/A
87
R45-2R4507-0921:00CharlotteRestriction45
PCR purification kit
Restriction
45pSB1C3-2ZNpuH6 (BsaI)H2O#N/A#N/A#N/A
88
R46-2R4607-0921:00CharlotteRestriction46
PCR purification kit
Restriction
46BBa_E0040_EcoR1_Spe1 (E/S)H2O#N/A#N/A#N/A
89
P18-2P1807-1019:30LisaPCR44
PCR purification kit
PP18BBa_E0040_EcoR1_Spe130.08017.5
90
R52R5207-10SilvanRestriction52
PCR purification kit
Restriction
52pSB1C3-2ZNpu (X/P)#N/A#N/A#N/A
91
R53R5307-10SilvanRestriction53
PCR purification kit
Restriction
53pSB1C3-2ZNpu (X/P)#N/A#N/A#N/A
92
R54R5407-10SilvanRestriction54
PCR purification kit
Restriction
54pSB1C3-2ZNpu (X/P)#N/A#N/A#N/A
93
R55R5507-10SilvanRestriction55
PCR purification kit
Restriction
55pSB1AK8-BBa_I712074 (S/P)#N/A#N/A#N/A
94
R56R5607-1214:00
Lisa + Consti
Gel21-2Gel extraction
Restriction
56pSB1C3-2ZSspH6 (BsaI)H2O30.01226.7
95
R57R5707-1214:00
Lisa + Consti
Gel21-3Gel extraction
Restriction
57pSB1C3-2ZNpuH6 (BsaI)H2O30.01826.8
96
R58R5807-1214:00
Lisa + Consti
Gel21-4Gel extraction
Restriction
58BBa_E0040_EcoR1_Spe1 (E/S)H2O30.03523.8
97
O47O4707-1214:35SilvanGrowth178C1
Qiagen miniprep kit
Plasmid4747pSB1AK8-T7-2ZNpu50.034647.054S47#3II2263, II2265,
98
P24P2407-1218:05SilvanPCR57
PCR purification kit
PP24BBa_746907_BsaI=EcoR1_His6_BsaI=Spe130.013527.0
99
P25P25
x/YYEs6gNX8ewyXfWGv
07-1217:00SilvanOligoAnnealingPP25BsaI=EcoRI_null_BsaI=SpeI100.097397.0
100
O48O4807-1420:30SilvanGrowth189C1
Qiagen miniprep kit
Plasmid4848pSB1AK8-T7-2ZNpu:(GFP-H6)50.028449.0G48II2264, II2266,
101
O45O4507-151:00LisaGrowth181C1
Qiagen miniprep kit
Plasmid4545pSB1C3-2ZNpuH6:GFPEB50.0100vertauschaungen!S45#4
102
O45-2O4507-151:00LisaGrowth181C2
Qiagen miniprep kit
Plasmid4545pSB1C3-2ZNpuH6:GFP50.070
vertauschaungen!, really bad, repeat, probaBIy not enough bacteria..
S45#4
103
O45-3O4507-151:00LisaGrowth181C3
Qiagen miniprep kit
Plasmid4545pSB1C3-2ZNpuH6:GFP50.0110vertauschaungen!S45#4
104
O44O4407-151:00LisaGrowth182C3
Qiagen miniprep kit
Plasmid4444pSB1C3-2ZSspH6:GFP50.0200vertauschaungen!S44#4
105
O44-2O4407-151:00LisaGrowth182C4
Qiagen miniprep kit
Plasmid4444pSB1C3-2ZSspH6:GFP50.090vertauschaungen!S44#4
106
O44-3O4407-151:00LisaGrowth182C5
Qiagen miniprep kit
Plasmid4444pSB1C3-2ZSspH6:GFP50.050vertauschaungen!S44#4
107
O45-4O4507-1516:30LisaGrowth181C1
Qiagen miniprep kit
Plasmid4545pSB1C3-2ZNpuH6:GFPEB30.01770vertauschaungen!S45#4
108
O44-4O4507-1516:30LisaGrowth181C2
Qiagen miniprep kit
Plasmid4445pSB1C3-2ZSspH6:GFP30.02190
vertauschaungen! previously O45-5, use 44-8
S45#4II2255, II2254no sequencing, we realized that "ist glaube ich Ssp_DnaE. hat funktioniert", besides that ok
109
O45-6O4507-1516:30LisaGrowth181C3
Qiagen miniprep kit
Plasmid4545pSB1C3-2ZNpuH6:GFP30.0920vertauschaungen!S45#4
110
O45-5O4407-1516:30LisaGrowth182C3
Qiagen miniprep kit
Plasmid4544pSB1C3-2ZNpuH6:GFP30.01670
vertauschaungen! previously O44-4, use 45-8
S44#4II2257, II2256no sequencing, we realized that this is npu (O45), has a mutation A1197del
111
O44-5O4407-1516:30LisaGrowth182C4
Qiagen miniprep kit
Plasmid4444pSB1C3-2ZSspH6:GFP30.0880vertauschaungen!S44#4
112
O44-6O4407-1516:30LisaGrowth182C5
Qiagen miniprep kit
Plasmid4444pSB1C3-2ZSspH6:GFP30.01660vertauschaungen!S44#4
113
R61R61
x/MRmcQNuEBtR8vQfuH
07-1423:00Max WOligoAnnealing
Restriction
61pSBX-T7
T4Ligase Buffer
100.040746.0cycler run 20
114
P30P30
s/3cByz57n4waxPDtB8
Distibution2014PP30pSB1A325-12
115
P31P31
s/HuGEqnxD4fRJekJnM
Distibution2014PP31pSB1C325-25
116
P32P32
s/qMGcNDnLGXXDM2e2o
Distibution2014PP32pSB1K325-25
117
P33P33
s/jhioKQSAgk9otaes2
Distibution2014PP33pSB1T325-25
118
O50O50
s/wnu2JTHbc5gqv4h3A
07-1518:00JanExternO2
from SYNtheSYS
Plasmid5050pSB4A5-BBa_J04450157-12.74
119
O51O51
s/SHQw48zRpXSsXpDBA
07-1518:00JanExternO17
from SYNtheSYS
Plasmid5151pSB4C5-BBa_J04450529-5.67
120
O52O52
s/yjpzG9aJGnavCw7jQ
07-1518:00JanExternO3
from SYNtheSYS
Plasmid5252pSB4K5-BBa_J04450102-17.8
121
O53O53
s/xK8jaWZ8EBRrD6vJJ
07-1518:00JanExternO12
from SYNtheSYS
Plasmid5353pSB1A2-BBa_I13507218-30.7
122
O49O49
s/xjP79KGLhyNgMWQLb
07-1518:00JanExternO27
from SYNtheSYS
Plasmid4949pSB1A3-BBa_J044501320
123
O54O54
s/jTBLe2Z37PN669Ti2
07-1518:00JanExternO28
from SYNtheSYS
Plasmid5454pSB1K3-BBa_J0445076-26.3
concentration not realistic, see Gel 36
124
O65O65
s/RseaZHbrsPG9KSpL2
07-1518:00JanExternO15
from SYNtheSYS
Plasmid6565pSB1C3-BBa_J044503210.0doesnt exist
125
O64O64
s/gCzWyGy7AuyfqJbkB
07-1518:00JanExternO16
from SYNtheSYS
Plasmid6464pSB1T3-BBa_J0445050.026938.8
126
O64-2O64
s/uqoZiCdHqbkddkxGq
07-2311:11JanExternO16b
from SYNtheSYS
Plasmid6464pSB1T3-BBa_J0445050.013735.4
127
O64-3O64
s/YeXamSC7qXWnA2j8L
07-2311:11JanExternO16'
from SYNtheSYS
Plasmid6464pSB1T3-BBa_J0445050.014936.5
128
R62R6207-1618:00CaroGel27-2-2,27-3-2Gel extraction
Restriction
62pSB1A2-BBa_I13507 (X/P)EB30.08-6.0estimated
129
R63R6307-1618:00CaroRestriction63
PCR purification kit
Restriction
63EB30.0212.3
130
R64R6407-1618:00CaroRestriction64
PCR purification kit
Restriction
640,EB30.067.5
131
R65R6507-1618:00CaroRestriction65
PCR purification kit
Restriction
65pSB1K3 (E/P)EB30.01921.5
132
R66R6607-1618:00CaroRestriction66
PCR purification kit
Restriction
66pSB1T3 (E/P)EB30.0821.6
133
R67R6707-1618:00CaroGel26-2-1,26-3-1Gel extraction
Restriction
67pSB4A5-BBa_J04450 (E/P)EB30.04420.7
134
R68R6807-1618:00CaroGel26-7-1,26-8-1Gel extraction
Restriction
68pSB4C5-BBa_J04450 (E/P)EB30.03820.7
135
R69R6907-1618:00CaroGel26-4-1,26-5-1,26-6-1Gel extraction
Restriction
69pSB4K5-BBa_J04450 (E/P)EB30.02320.8
136
R70R7007-1618:00CaroGel26-9-1,26-10-1Gel extraction
Restriction
70pSB1A3-BBa_J04450 (E/P)EB30.03321.4
137
R71R7107-1618:00CaroGel26-11-1,26-12-1Gel extraction
Restriction
71pSB1K3-BBa_J04450 (E/P)EB30.02121.2
138
R72R7207-1718:00CaroGel28-2-2,28-3-2Gel extraction
Restriction
72pSB1A2-BBa_I13507 (X/P)EB50.05-15.3
139
O23-3O2307-1718:45MaggiePurificationO23DilutionPlasmid2323pRSFDuet-1-mDNMT1(731-1602)191.01160.0missing
140
O49-2O4907-1718:45MaggiePurificationO49DilutionPlasmid4949pSB1A3-BBa_J04450131.51126.5
141
R61-2R6107-181:50SilvanPurificationR61Dilution
Restriction
61T7promH2O100.0487.24419514
142
P36P3607-2015:00MaggieGel
32-3-2.2kb,32-3-2.2kb
Gel extractionPP36pSB1A3_SortAc-H6_Smt330.02429.0342
143
P35P3507-2015:00MaggieGel
32-6-778bp,32-7-778bp
Gel extractionPP35ccdB_Smt3-TEVc-BsaI_SortAc-H6-RFC10suffix30.0329.0778
144
P34P3407-2015:00MaggieGel
32-9-342bp,32-10-342bp
Gel extractionPP34Smt3_RFC10prefix_TEVc-BsaI30.05829.02207
145
P36-2P3607-20MaggieGel
34-1-2.2kb,34-3-2.2kb
Gel extractionPP36pSB1A3_SortAc-H6_Smt330.02429.0
146
P35-2P3507-20MaggieGel
34-4-778bp,34-5-778bp
Gel extractionPP35ccdB_Smt3-TEVc-BsaI_SortAc-H6-RFC10suffix30.03229.0
147
P34-2P3407-20MaggieGel
34-6-342bp,34-7-342bp
Gel extractionPP34Smt3_RFC10prefix_TEVc-BsaI30.05729.0
148
O59O5907-2012:00CaroGrowth205C4
Qiagen miniprep kit
Plasmid5959pSBX1K3-BBa_I13507H2O1440L59#3II2258bad sequencing
149
O58O5807-2012:00CaroGrowth194C1
Qiagen miniprep kit
Plasmid5858pSBX1C3-BBa_I13507H2O1000L58II2259bad sequencing
150
O46O4607-2012:00CaroGrowth175C1-1
Qiagen miniprep kit
Plasmid4646pSB1C3-BBa_S03518H2O1040
D2014-3-21G
151
O57O5707-2012:00CaroGrowth203C1
Qiagen miniprep kit
Plasmid5757pSBX1A3-BBa_I13507H2O1300L57#3II2260bad sequencing
152
O36-2O3607-2012:00CaroGrowth180C2-2
Qiagen miniprep kit
Plasmid3636pSB1AK8-BBa_I712074H2O1220.0O36
153
O59-2O5907-2012:00CaroGrowth195C1
Qiagen miniprep kit
Plasmid5959pSBX1K3-BBa_I13507H2O760.0L59II2261bad sequencing
154
O58-2O5807-2012:00CaroGrowth204C2
Qiagen miniprep kit
Plasmid5858pSBX1C3-BBa_I13507H2O1340L58#2II2262pre insert insert containing ecoRI site
155
O22-0
x/8hoekt4dMb7JGqjEL
06-2312:00
Maggie, Silvan
ExternBashtrykovPlasmid2222pFastBac-mDNMT10.0
156
O23-0
x/tDBSAGTGnyvvt7Yha
06-2312:00
Maggie, Silvan
ExternBashtrykovPlasmid2323pRSFDuet-1-mDNMT1(731-1602)0.0
157
O24-0
s/jbP73zoHFnALQE7MF
06-2312:00
Maggie, Silvan
ExternMootzPlasmid2424pIT770.0
158
O25-0
s/aEM4DJSRXsAcMeANd
06-2312:00
Maggie, Silvan
ExternMootzPlasmid2525pIT290.0
159
O26-0
s/LsHLEn8gCMyhaQg2a
06-2312:00
Maggie, Silvan
ExternMootzPlasmid2626pIT210.0
160
O27-0
s/zoGDXDeT9Rj2RJ5gm
06-2312:00
Maggie, Silvan
ExternMootzPlasmid2727pCL200.0
161
O28-0
s/XzdfJqK3fgcXdKH53
06-2312:00
Maggie, Silvan
ExternMootzPlasmid2828pAU070.0
162
O29-0
s/ajbhi77D2HWANBKqr
06-2312:00
Maggie, Silvan
ExternMootzPlasmid2929pVS410.0
163
O30-0
s/CsQ3wGyhTFiLRGJPG
06-2312:00
Maggie, Silvan
ExternMootzPlasmid3030pVS070.0
164
O55O5507-2216:30SilvanGrowth211C2
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)EB50.0310-6.956A55II2267, II2268,
165
R73R7307-231:40JanGel36-2-1Gel extraction
Restriction
73pSB1A3-BBa_J04450 (E/P)EB30.0623.522.28081059
166
R74R7407-231:40JanGel36-3-1Gel extraction
Restriction
74pSB1C3-BBa_I15017 (E/P)EB30.01125.024.53769559
167
R75R7507-231:40JanGel36-4-1Gel extraction
Restriction
75pSB1K3-BBa_J04450 (E/P)EB30.0322.42
168
R76R7607-231:40JanGel36-5-1Gel extraction
Restriction
76pSB1T3-BBa_J04450 (E/P)EB30.0824.0
is it really all 1T3 ? 22.4462776250127
169
R77R7707-231:40JanGel36-6-1Gel extraction
Restriction
77pSB4A5-BBa_J04450 (E/P)EB30.01023.725.39364231
170
R78R7807-231:40JanGel36-7-1Gel extraction
Restriction
78pSB4C5-BBa_J04450 (E/P)EB30.0923.525.35095579
171
R79R7907-231:40JanGel36-8-1Gel extraction
Restriction
79pSB4K5-BBa_J04450 (E/P)EB30.01124.025.02719503
172
R80R8007-231:40JanGel36-9-2,36-10-2Gel extraction
Restriction
80pSB1A2-BBa_I13507 (X/P)EB30.09-1.524.4047619
173
R77-2R7707-231:40JanGel36-6-2,36-7-2,36-8-2Gel extraction
Restriction
77pSB4A5-BBa_J04450 (E/P)EB30.0828.023.81907308
174
R83R8307-2418:00JanGel38-2-1,38-3-1Gel extraction
Restriction
83pSB1T3-BBa_J04450 (E/S)EB30.01024.4
nanodrop did measure 75 ng/µL 13.943342776204
175
R84R8407-2418:00JanGel38-4-1,38-5-2Gel extraction
Restriction
84pSB1T3-BBa_J04450 (E/P)EB30.01028.013.94
176
O55-2O5507-2418:38SilvanGrowth209C2
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)EB50.08648.057A55II2269
177
O55-3O5507-2418:38SilvanGrowth211C1
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)EB50.029248.058A55II2270
178
O55-4O5507-2418:38SilvanGrowth212C1
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)EB50.019827.859A55#2II2271
179
O49-3O4907-2423:00SilvanGrowth217C1
Qiagen miniprep kit
Plasmid4949pSB1A3-BBa_J04450EB50.011048.062O49
180
O50-2O5007-2423:00SilvanGrowth218C1
Qiagen miniprep kit
Plasmid5050pSB4A5-BBa_J04450EB50.010748.061O50
181
O51-2O5107-2423:00SilvanGrowth219C1
Qiagen miniprep kit
Plasmid5151pSB4C5-BBa_J04450EB50.031125.563O51
182
O52-2O5207-2423:00SilvanGrowth220C1
Qiagen miniprep kit
Plasmid5252pSB4K5-BBa_J04450EB50.05347.864O52
183
O53-2O5307-2423:00SilvanGrowth221C1
Qiagen miniprep kit
Plasmid5353pSB1A2-BBa_I13507EB50.012932.560O53
184
O54-2O5407-2423:00SilvanGrowth222C1
Qiagen miniprep kit
Plasmid5454pSB1K3-BBa_J04450EB50.010348.065O54
185
O66O6607-2423:00SilvanGrowth224C1
Qiagen miniprep kit
Plasmid6666pSB3T5-BBa_J04450EB50.012930.567D2014-4-8D
186
O64-4O6407-2423:00SilvanGrowth227C1
Qiagen miniprep kit
Plasmid6464pSB1T3-BBa_J04450EB50.08524.566O64-2
187
R85R85
x/gfsFTzZFZNTLMd4RX
07-2520:00MaxWOligoAnnealing
Restriction
85T7RBS_BioBrick_v2T4L1,000.032999.0
cycler run 27, prev thought to be
188
R86R86
x/sLge6jeo2xtv3ptz5
07-2520:00MaxWOligoAnnealing
Restriction
86T7RBS-IGEMHD_v3T4L1,000.052980.0cycler run 28
189
R87R87
x/wnWjSFKNHbx3ghRsj
07-2520:00MaxWOligoAnnealing
Restriction
87AssemBIyIntN-linkerT4L1,000.0101,000.0cycler run 29
190
R88R88
x/Fbi9JGJKiYiRADvWx
07-2520:00MaxWOligoAnnealing
Restriction
88AssemBIyIntC-linkerT4L1,000.0101,000.0cycler run 30
191
O57-2O5707-2614:50
Jan, Max H
Growth234C1
Qiagen miniprep kit
Plasmid5757pSBX1A3-BBa_I13507EB50.091068empty(to be used further)L57#5FG1165, FG1198sequenzing starts ok, then mixture?, ok
192
O58-3O5807-2614:50
Jan, Max H
Growth235C1
Qiagen miniprep kit
Plasmid5858pSBX1C3-BBa_I13507EB50.013647.869L58#3
193
O59-3O5907-2614:50
Jan, Max H
Growth236C1
Qiagen miniprep kit
Plasmid5959pSBX1K3-BBa_I13507EB50.013232.670to be used furtherL59#5FG1167, FG1177, FG1199frameshift, two fucking huge insertions, ok,longest,
194
O61O6107-2614:50
Jan, Max H
Growth238C1
Qiagen miniprep kit
Plasmid6161pSBX4A5-BBa_I13507EB50.0108071to be used furtherL61#3FG1169starts ok then mixture
195
O62O6207-2614:50
Jan, Max H
Growth239C1
Qiagen miniprep kit
Plasmid6262pSBX4C5-BBa_I13507EB50.057037.372to be used furtherL62#3FG1170ok
196
O63O6307-2614:50
Jan, Max H
Growth240C1
Qiagen miniprep kit
Plasmid6363pSBX4K5-BBa_I13507EB50.010147.873discardedL63#3FG1187bad sequencing
197
O57-3O5707-2614:50
Jan, Max H
Growth234C2
Qiagen miniprep kit
Plasmid5757pSBX1A3-BBa_I13507EB50.08347.874discardL57#5FG1173bad sequencing
198
O58-4O5807-2614:50
Jan, Max H
Growth235C2
Qiagen miniprep kit
Plasmid5858pSBX1C3-BBa_I13507EB50.04946.875L58#3FG1175ok
199
O59-4O5907-2614:50
Jan, Max H
Growth236C2
Qiagen miniprep kit
Plasmid5959pSBX1K3-BBa_I13507EB50.011225.576L59#5FG1178ok
200
O61-2O6107-2614:50
Jan, Max H
Growth238C2
Qiagen miniprep kit
Plasmid6161pSBX4A5-BBa_I13507EB50.047077discardedL61#3FG1181bad sequencing
201
O62-2O6207-2614:50
Jan, Max H
Growth239C2
Qiagen miniprep kit
Plasmid6262pSBX4C5-BBa_I13507EB50.032647.878L62#3
202
O63-2O6307-2614:50
Jan, Max H
Growth240C2
Qiagen miniprep kit
Plasmid6363pSBX4K5-BBa_I13507EB50.055079L63#3FG1171no sequenzing
203
O57-4O5707-2614:50
Jan, Max H
Growth234C3
Qiagen miniprep kit
Plasmid5757pSBX1A3-BBa_I13507EB50.07647.880discardL57#5FG1174bad sequencing
204
O58-5O5807-2614:50
Jan, Max H
Growth235C3
Qiagen miniprep kit
Plasmid5858pSBX1C3-BBa_I13507EB50.034081to be used furtherL58#3FG1166bad sequenzing
205
O59-5O5907-2614:50
Jan, Max H
Growth236C3
Qiagen miniprep kit
Plasmid5959pSBX1K3-BBa_I13507EB50.013347.882L59#5FG1179ok, short read
206
O61-3O6107-2614:50
Jan, Max H
Growth238C3
Qiagen miniprep kit
Plasmid6161pSBX4A5-BBa_I13507EB50.068083discardedL61#3FG1182bad sequencing
207
O62-3O6207-2614:50
Jan, Max H
Growth239C3
Qiagen miniprep kit
Plasmid6262pSBX4C5-BBa_I13507EB50.021443.184L62#3
208
O63-3O6307-2614:50
Jan, Max H
Growth240C3
Qiagen miniprep kit
Plasmid6363pSBX4K5-BBa_I13507EB50.04047.885to be used furtherL63#3FG1188bad sequencing
209
O65-2O65
s/Csi2jWhAjfYrFvcWQ
06-0115:30DistributionPlasmid6565pSB1C3-BBa_J0445000.0
210
O65-3O65
s/q6QmqRNBd4AcT6Z4j
06-0115:30DistributionPlasmid6565pSB1C3-BBa_J0445000.0
211
O65-4O65
s/bYEeipaiNwsmYDJG3
06-0115:30DistributionPlasmid6565pSB1C3-BBa_J0445000.0
212
O65-5O65
s/Dbq95DeScbMpSi5EW
06-0115:30DistributionPlasmid6565pSB1C3-BBa_J0445000.0
213
O65-6O65
s/Kmjb927eeqrLKE68G
06-0115:30DistributionPlasmid6565pSB1C3-BBa_J0445000.0
214
R94R9407-2711:40
Anna, Jakob
Gel43-2-1Gel extraction
Restriction
94pSB1T3-BBa_J04450 (E/X)EB30.0830.08.316666667
215
R94-2R9407-2711:40
Anna, Jakob
Gel43-3-1Gel extraction
Restriction
94pSB1T3-BBa_J04450 (E/X)EB30.0814.3249.5
216
R95R9507-2711:40
Anna, Jakob
Gel43-4-1Gel extraction
Restriction
95pSB1T3-BBa_J04450 (E/P)EB30.0630.05.766666667
217
R95-2R9507-2711:40
Anna, Jakob
Gel43-5-1Gel extraction
Restriction
95pSB1T3-BBa_J04450 (E/P)EB30.0625.2
218
R96R9607-2711:40
Anna, Jakob
Gel43-6-2Gel extraction
Restriction
96pSB1A2-BBa_I13507 (X/P)EB30.0330.02.5
219
R96-2R9607-2711:40
Anna, Jakob
Gel43-7-2Gel extraction
Restriction
96pSB1A2-BBa_I13507 (X/P)EB30.0318.0
220
R97R9707-2711:40
Anna, Jakob
Gel43-8-1Gel extraction
Restriction
97pSB3T5-BBa_J04450 (E/X)EB30.0830.08.283333333
221
R97-2R9707-2711:40
Anna, Jakob
Gel43-9-1Gel extraction
Restriction
97pSB3T5-BBa_J04450 (E/X)EB30.0830.0
222
R61-3R6107-280:30JakobPurificationR61Dilution
Restriction
61T7promH2O100.0998.5
223
O60O6007-280:30JanGrowth242C7
Qiagen miniprep kit
Plasmid6060pSBX1T3-BBa_I13507EB50.07924.5L60#3FG1168ok
224
O60-2O6007-280:30JanGrowth242C8
Qiagen miniprep kit
Plasmid6060pSBX1T3-BBa_I13507EB50.014035.7L60#3
225
L0L0
s/pMavcx63HXFvya3st
07-2512:00MaxWExtern?ask Holger
Lambda DNA
0?
storage buffer
100.0183.0
226
O30-2O3007-2823:00
Constantin
PurificationO30DilutionPlasmid3030pVS07H2O151.01140.0
227
O29-2O2907-2823:00
Constantin
PurificationO29DilutionPlasmid2929pVS41H2O114.01104.0
228
R98R9807-2823:30JakobGel44-2-1,44-3-1Gel extraction
Restriction
98pSBX1A3-BBa_I13507 (E/S)EB30.02219.8
229
R99R9907-2823:30JakobGel44-4-1,44-5-1Gel extraction
Restriction
99pSBX1C3-BBa_I13507 (E/S)EB30.0136.2
230
R100R10007-2823:30JakobGel44-6-1,44-7-1Gel extraction
Restriction
100pSBX1K3-BBa_I13507 (E/S)EB30.01914.2
231
R101R10107-2823:30JakobGel44-8-1,44-9-1Gel extraction
Restriction
101pSBX1T3-BBa_I13507 (E/S)EB30.08-1.8
232
R102R10207-2823:30JakobGel44-10-1,44-11-1Gel extraction
Restriction
102pSBX4A5-BBa_I13507 (E/S)EB30.01516.5
233
R103R10307-2823:30JakobGel44-13-1,44-14-1Gel extraction
Restriction
103pSBX4C5-BBa_I13507 (E/S)EB30.01814.2
234
R104R10407-2823:30JakobGel44-15-1,44-16-1Gel extraction
Restriction
104pSBX4K5-BBa_I13507 (E/S)EB30.074.9
235
R105R10507-2823:30JakobGel44-17-1,44-18-1Gel extraction
Restriction
105pSB1C3-2ZSspH6:GFP (E/S)EB30.01323.9
236
R106R10607-2823:30JakobGel44-19-1,44-20-1Gel extraction
Restriction
106pSB1C3-2ZNpuH6:GFP (E/S)EB30.01123.0
237
R107R10707-2920:30CharlotteGel46-9-1Gel extraction
Restriction
107pSB1C3-2ZSspH6:GFP (E/S)H2O30.01119.5
238
R107-2R10707-2920:30CharlotteGel46-10-1Gel extraction
Restriction
107pSB1C3-2ZSspH6:GFP (E/S)H2O30.01930.0
239
R108R10807-2920:30CharlotteGel46-11-1Gel extraction
Restriction
108pSB1C3-2ZNpuH6:GFP (E/S)H2O30.0919.3
240
R108-2R10807-2920:30CharlotteGel46-12-1Gel extraction
Restriction
108pSB1C3-2ZNpuH6:GFP (E/S)H2O30.09630.0
241
P46P4607-29AnnaGel45-2Gel extractionPP46Lambda-Lys_pSBX_pSBXH2O30.01630.0
242
P46-2P4607-29AnnaGel45-3Gel extractionPP46Lambda-Lys_pSBX_pSBXH2O30.01030.0
243
P47P4707-29AnnaGel47-2Gel extractionPP47GFP_pSBX_pSBXH2O30.01630.0
244
P47-2P4707-29AnnaGel48-2Gel extractionPP47GFP_pSBX_pSBXH2O30.01830.0
245
P48P4807-29AnnaGel48-3Gel extractionPP48NpuDnaEN_H2O30.07030.0
246
P48-2P4807-29AnnaGel48-4Gel extractionPP48NpuDnaEN_H2O30.04230.0
247
P49P4907-29AnnaGel48-5Gel extractionPP49NpuDnaEC_H2O30.05030.0
248
P49-2P4907-29AnnaGel48-6Gel extractionPP49NpuDnaEC_H2O30.03330.0
249
P44P4407-29AnnaGel48-7Gel extractionPP44ccdB_BsmBI_BsmBIH2O30.04530.0
250
P44-2P4407-29AnnaGel48-8Gel extractionPP44ccdB_BsmBI_BsmBIH2O30.06530.0
251
P45P4507-29AnnaGel48-9Gel extractionPP45mRFP_BsaI_BsaIH2O30.03730.0
252
P45-2P4507-29AnnaGel48-10Gel extractionPP45mRFP_BsaI_BsaIH2O30.05030.0
253
O80O8007-3123:00CharlotteGrowth279C1
Qiagen miniprep kit
Plasmid8080pSBX4C5-2ZSspH6:GFPEB30.0015.087discardedS80
254
O80-2O8007-3123:00CharlotteGrowth279C2
Qiagen miniprep kit
Plasmid8080pSBX4C5-2ZSspH6:GFPEB30.030723.5discardedS80
255
O81O8108-0111:00CharlotteGrowth280C2
Qiagen miniprep kit
Plasmid8181pSBX4C5-2ZNpuH6:GFPEB30.0030.0S81
256
O43-2O4308-0111:00CharlotteGrowth164C1-1
Qiagen miniprep kit
Plasmid4343pSB1C3-BBa_I746907EB30.000D2014-3-12I
257
O77O7708-0218:00CaroGrowth297C1
Qiagen miniprep kit
Plasmid7777pSBX1C3-T7RBS_BBv2H2O30.058630.089S77
258
O78O7808-0218:00CaroGrowth289C1
Qiagen miniprep kit
Plasmid7878pSBX1K3-T7RBS_BBv2H2O30.02199.890S7876DB35seems ok; some bases badly called
259
O78-2O7808-0218:00CaroGrowth289C2
Qiagen miniprep kit
Plasmid7878pSBX1K3-T7RBS_BBv2H2O30.013611.691S78
260
O78-3O7808-0218:00CaroGrowth289C3
Qiagen miniprep kit
Plasmid7878pSBX1K3-T7RBS_BBv2H2O30.015523.592S78
261
O72O7208-0218:00CaroGrowth285C1
Qiagen miniprep kit
Plasmid7272pSBX4A5-T7RBS-IGEMHD_v3H2O30.018430.093S72#2FG1191good sequencing, ok
262
O72-2O7208-0218:00CaroGrowth294C1
Qiagen miniprep kit
Plasmid7272pSBX4A5-T7RBS-IGEMHD_v3H2O30.0129094discardedS72#2
263
O70O7008-0218:00CaroGrowth292C1
Qiagen miniprep kit
Plasmid7070pSBX1K3-T7RBS-IGEMHD_v3H2O30.010530.095S70#2
264
O70-2O7008-0218:00CaroGrowth292C2
Qiagen miniprep kit
Plasmid7070pSBX1K3-T7RBS-IGEMHD_v3H2O30.010329.096S70#2
265
O72-3O7208-0220:00CaroPurificationO72DilutionPlasmid7272pSBX4A5-T7RBS-IGEMHD_v3H2O184.010discarded
266
O72-4O7208-0220:00CaroPurificationO72-2DilutionPlasmid7272pSBX4A5-T7RBS-IGEMHD_v3H2O129.010discarded
267
O70-3O7008-0220:00CaroPurificationO70DilutionPlasmid7070pSBX1K3-T7RBS-IGEMHD_v3H2O105.01104.0
268
O70-4O7008-0220:00CaroPurificationO70-2DilutionPlasmid7070pSBX1K3-T7RBS-IGEMHD_v3H2O103.0192.0sequenzed descendands
269
R111R11108-0223:00JanGel50-2-1,50-3-1Gel extraction
Restriction
111pSBX1T3-BBa_I13507 (E/S)H2O30.01023.6
estimated concentration, used wrong columns
270
R112R11208-0223:00JanGel50-4-1,50-5-1Gel extraction
Restriction
112pSBX4C5-BBa_I13507 (E/S)H2O30.01123.5
estimated concentration, used wrong columns
271
P61P6108-031:00MaxWGel52-6, 52-7Gel extractionPP61pSBX4A5_taataa_atgH2O30.0530.0
272
P60P6008-0314:00CaroGel53-2,53-3,53-4Gel extractionPP60pSBX4A5_mRFP-BsaI_BsmBI-ccdBH2O30.01630.0
273
P59P5908-0314:00CaroGel53-6Gel extractionPP59pSBX4A5_ccdB-BsmBI_BsaI-mRFPH2O30.06330.0
274
P59-2P5908-0314:00CaroGel53-7,53-8Gel extractionPP59pSBX4A5_ccdB-BsmBI_BsaI-mRFPH2O30.010530.0
275
O58-6O5808-0315:31AnnaGrowth235C3-1
Qiagen miniprep kit
Plasmid5858pSBX1C3-BBa_I13507H2O30.0426.1L58#3FG1176bad sequencing
276
O61-4O6108-0315:31AnnaGrowth238C1-1
Qiagen miniprep kit
Plasmid6161pSBX4A5-BBa_I13507H2O30.01090L61#3FG1183ok
277
O63-4O6308-0315:31AnnaGrowth240C2-1
Qiagen miniprep kit
Plasmid6363pSBX4K5-BBa_I13507H2O30.07225.8L63#3FG1189ok
278
O62-4O6208-0315:31AnnaGrowth239C1-1
Qiagen miniprep kit
Plasmid6262pSBX4C5-BBa_I13507H2O30.011230.0L62#3
279
O60-3O6008-0315:31AnnaGrowth242C13
Qiagen miniprep kit
Plasmid6060pSBX1T3-BBa_I13507H2O30.0804.098L60#3FG1180ok
280
R113R11308-042:00JakobGel56-2-1, 56-3-1Gel extraction
Restriction
113pSB1AK8-BBa_I712074 (S/P)H2O30.01624.7
281
R114R11408-042:00JakobGel56-4-2, 56-5-2Gel extraction
Restriction
114pSB1C3-2ZSspH6:GFP (X/P)H2O30.01525.9
282
R115R11508-042:00JakobGel56-6-2, 56-7-2Gel extraction
Restriction
115pSB1C3-2ZNpuH6:GFP (X/P)H2O30.01626.0
283
O62-5O6208-0417:22AnnaGrowth327C1
Qiagen miniprep kit
Plasmid6262pSBX4C5-BBa_I13507H2O50.050.099O62
284
O62-6O6208-0417:22AnnaGrowth327C2
Qiagen miniprep kit
Plasmid6262pSBX4C5-BBa_I13507H2O50.05331.0100
2 Tage lang induziert -> Fehler?
O62FG1184ok, longest
285
O62-7O6208-0417:22AnnaGrowth327C3
Qiagen miniprep kit
Plasmid6262pSBX4C5-BBa_I13507H2O50.050.0101O62
286
O62-8O6208-0417:22AnnaGrowth327C4
Qiagen miniprep kit
Plasmid6262pSBX4C5-BBa_I13507H2O50.0050.0102O62
287
O62-9O6208-0417:22AnnaGrowth327C5
Qiagen miniprep kit
Plasmid6262pSBX4C5-BBa_I13507H2O50.02050.0103
2 Tage lang induziert -> Fehler?
O62FG1185ok
288
O62-10O6208-0417:22AnnaGrowth327C6
Qiagen miniprep kit
Plasmid6262pSBX4C5-BBa_I13507H2O50.07222.2104
2 Tage lang induziert -> Fehler?
O62FG1186ok
289
O43-3O4308-059:00CharlotteGrowth164C1-2
Qiagen miniprep kit
Plasmid4343pSB1C3-BBa_I746907H2O50.0050.0D2014-3-12I
290
O44-7O4408-059:00LisaGrowth347C1
Qiagen miniprep kit
Plasmid4444pSB1C3-2ZSspH6:GFPH2O30.0030.0O44-4
291
O44-8O4408-059:00LisaGrowth347C2
Qiagen miniprep kit
Plasmid4444pSB1C3-2ZSspH6:GFPH2O30.0030.0110O44-4
292
O45-7O4508-059:00LisaGrowth348C1
Qiagen miniprep kit
Plasmid4545pSB1C3-2ZNpuH6:GFPH2O30.0030.0O45-5
293
O45-8O4508-059:00LisaGrowth348C2
Qiagen miniprep kit
Plasmid4545pSB1C3-2ZNpuH6:GFPH2O30.0030.0108O45-5
294
O36-3O3608-059:00LisaGrowth349C1
Qiagen miniprep kit
Plasmid3636pSB1AK8-BBa_I712074H2O30.012121.7109O36
295
O36-4O3608-059:00LisaGrowth349C2
Qiagen miniprep kit
Plasmid3636pSB1AK8-BBa_I712074H2O30.012312.1O36
296
O71O7108-059:00AnnaGrowth328C2
Qiagen miniprep kit
Plasmid7171pSBX1T3-T7RBS-IGEMHD_v3H2O50.03250.0
Sind 2 Tage lang gewachsen -> Fehler?
S71#3
297
O71-2O7108-059:00AnnaGrowth328C3
Qiagen miniprep kit
Plasmid7171pSBX1T3-T7RBS-IGEMHD_v3H2O50.05732.5
Sind 2 Tage lang gewachsen -> Fehler?
S71#3FG1190good sequencing, ok
298
O73O7308-059:00AnnaGrowth329C2
Qiagen miniprep kit
Plasmid7373pSBX4C5-T7RBS-IGEMHD_v3H2O50.043750.0
Sind 2 Tage lang gewachsen -> Fehler?
S73#3FG1192good sequencing, ok
299
O73-2O7308-059:00AnnaGrowth329C3
Qiagen miniprep kit
Plasmid7373pSBX4C5-T7RBS-IGEMHD_v3H2O50.041145.1
Sind 2 Tage lang gewachsen -> Fehler?
S73#3
300
O71-3O7108-0513:41MaxWPurificationO71-2DilutionPlasmid7171pSBX1T3-T7RBS-IGEMHD_v3H2O57.0145.0
301
O73-3O7308-0513:41MaxWPurificationO73DilutionPlasmid7373pSBX4C5-T7RBS-IGEMHD_v3H2O437.010discarded
302
P61-2P6108-0517:37MaxWGel65-2Gel extractionPP61pSBX4A5_taataa_atgH2O30.02030.0
303
P73P7308-0517:37MaxWGel65-3Gel extractionPP73pSBX1T3_taataa_atgH2O30.01330.0
304
P74P7408-0517:37MaxWGel65-4Gel extractionPP74pSBX4C5_taataa_atgH2O30.01530.0
305
P69P6908-0520:00JakobGel66-2, 66-3, 66-4Gel extractionPP69pSBX1T3_ccdB-BsmBI_BsaI-mRFPH2O30.01530.0
306
P70P7008-0520:00JakobGel66-5, 66-6, 66-7Gel extractionPP70pSBX1T3_mRFP-BsaI_BsmBI-ccdBH2O30.01530.0
307
P71P7108-0520:00JakobGel66-8, 66-9, 66-10Gel extractionPP71pSBX4C5_ccdB-BsmBI_BsaI-mRFP_NH2O30.01530.0
308
P72P7208-0520:00JakobGel66-11, 66-12, 66-13Gel extractionPP72pSBX4C5_mRFP-BsaI_BsmBI-ccdB_CH2O30.01530.0
309
P75P7508-0601:28MaxWGel67-2, 67-3Gel extractionPP75NpuDnaEC_T7_RBS-BsaI_mRFPH2O150.0
310
P76P7608-0601:28MaxWGel67-4, 67-5Gel extractionPP76NpuDnaEN_mRFP_BsaI-taataa_iGEMHDH2O30.05030.0
311
O65-7O6508-0612:45CaroGrowth366C1
Qiagen miniprep kit
Plasmid6565pSB1C3-BBa_J04450H2O30.08621.3D2014-4-4BFG1193ok
312
O65-8O6508-0612:45CaroGrowth366C2
Qiagen miniprep kit
Plasmid6565pSB1C3-BBa_J04450H2O30.07130.0D2014-4-4B
313
O65-9O6508-0612:45CaroGrowth366C3
Qiagen miniprep kit
Plasmid6565pSB1C3-BBa_J04450H2O30.012230.0D2014-4-4B
314
O88O8808-0612:45CaroGrowth367C1
Qiagen miniprep kit
Plasmid8888pSB1C3-BBa_J52008H2O30.07030.0D2014-3-3JFG1194ok
315
O88-2O8808-0612:45CaroGrowth367C2
Qiagen miniprep kit
Plasmid8888pSB1C3-BBa_J52008H2O30.09730.0D2014-3-3J
316
O88-3O8808-0612:45CaroGrowth367C3
Qiagen miniprep kit
Plasmid8888pSB1C3-BBa_J52008H2O30.09030.0D2014-3-3J
317
O89O8908-0612:45CaroGrowth368C1
Qiagen miniprep kit
Plasmid8989pSB1AK8-BBa_I712019H2O30.017530.0
D2014-4-20C
FG1195ok, good
318
O89-2O8908-0612:45CaroGrowth368C2
Qiagen miniprep kit
Plasmid8989pSB1AK8-BBa_I712019H2O30.020830.0
D2014-4-20C
319
O89-3O8908-0612:45CaroGrowth368C3
Qiagen miniprep kit
Plasmid8989pSB1AK8-BBa_I712019H2O30.015930.0
D2014-4-20C
320
O90O9008-0612:45CaroGrowth369C1
Qiagen miniprep kit
Plasmid9090pSB1C3-BBa_J06702H2O30.07730.0
D2014-3-15B
FG1196ok, good
321
O90-2O9008-0612:45CaroGrowth369C2
Qiagen miniprep kit
Plasmid9090pSB1C3-BBa_J06702H2O30.0820discarded
D2014-3-15B
322
O90-3O9008-0612:45CaroGrowth369C3
Qiagen miniprep kit
Plasmid9090pSB1C3-BBa_J06702H2O30.0650discarded
D2014-3-15B
323
O91O9108-0612:45CaroGrowth370C1
Qiagen miniprep kit
Plasmid9191pSB1A2-BBa_E0040H2O30.012030.0D2014-4-13LFG1197ok, good
324
O91-2O9108-0612:45CaroGrowth370C2
Qiagen miniprep kit
Plasmid9191pSB1A2-BBa_E0040H2O30.0860discardedD2014-4-13L
325
O91-3O9108-0612:45CaroGrowth370C3
Qiagen miniprep kit
Plasmid9191pSB1A2-BBa_E0040H2O30.01580discardedD2014-4-13L
326
O60-4O6008-0622:00AnnaGrowth325C6
Qiagen miniprep kit
Plasmid6060pSBX1T3-BBa_I13507H2O50.0050.0
Bin mir nicht ganz sicher, was drin ist. Besser nicht verwenden.
O60
327
O60-5O6008-0622:00AnnaGrowth326C1
Qiagen miniprep kit
Plasmid6060pSBX1T3-BBa_I13507H2O100.00100.0
Bin mir nicht ganz sicher, was drin ist. Besser nicht verwenden.
O60
328
O59-6O5908-0811:51AnnaGrowth384C1
Qiagen miniprep kit
Plasmid5959pSBX1K3-BBa_I13507H2O50.02050.0133O59-3
329
O59-7O5908-0811:51AnnaGrowth384C2
Qiagen miniprep kit
Plasmid5959pSBX1K3-BBa_I13507H2O50.02650.0134O59-3
330
O59-8O5908-0811:51AnnaGrowth384C3
Qiagen miniprep kit
Plasmid5959pSBX1K3-BBa_I13507H2O50.01450.0135O59-3
331
O59-9O5908-0811:51AnnaGrowth385C1
Qiagen miniprep kit
Plasmid5959pSBX1K3-BBa_I13507H2O50.01750.0136O59-5
332
O59-10O5908-0811:51AnnaGrowth385C2
Qiagen miniprep kit
Plasmid5959pSBX1K3-BBa_I13507H2O50.01650.0137O59-5
333
O59-11O5908-0811:51AnnaGrowth385C3
Qiagen miniprep kit
Plasmid5959pSBX1K3-BBa_I13507H2O50.0050.0138O59-5
334
O61-5O6108-0811:51AnnaGrowth386C2
Qiagen miniprep kit
Plasmid6161pSBX4A5-BBa_I13507H2O50.01729.0140O61-4
335
O61-6O6108-0811:51AnnaGrowth386C3
Qiagen miniprep kit
Plasmid6161pSBX4A5-BBa_I13507H2O50.0248.8141O61-4
336
O63-5O6308-0811:51AnnaGrowth387C1
Qiagen miniprep kit
Plasmid6363pSBX4K5-BBa_I13507H2O50.0100142discardedO63-4
337
O63-6O6308-0811:51AnnaGrowth387C3
Qiagen miniprep kit
Plasmid6363pSBX4K5-BBa_I13507H2O50.0270144discardedO63-4
340
O71-5O7108-0814:30JakobPurificationO71-2DilutionPlasmid7171pSBX1T3-T7RBS-IGEMHD_v3H2O57.2010discarded
341
O72-5O7208-0814:30JakobPurificationO72DilutionPlasmid7272pSBX4A5-T7RBS-IGEMHD_v3H2O184.101180.10
342
O73-5O7308-0814:30JakobPurificationO73DilutionPlasmid7373pSBX4C5-T7RBS-IGEMHD_v3H2O436.601420.60
343
O65-10O6508-0814:30JakobPurificationO65-7DilutionPlasmid6565pSB1C3-BBa_J04450H2O86.00174.00
344
O88-4O8808-0814:30JakobPurificationO88DilutionPlasmid8888pSB1C3-BBa_J52008H2O70.00168.00
345
O89-4O8908-0814:30JakobPurificationO89DilutionPlasmid8989pSB1AK8-BBa_I712019H2O175.001173.00
346
O90-4O9008-0814:30JakobPurificationO90DilutionPlasmid9090pSB1C3-BBa_J06702H2O77.00171.00
347
O91-4O9108-0814:30JakobPurificationO91DilutionPlasmid9191pSB1A2-BBa_E0040H2O120.001110.00
348
P46-3P4608-0817:45CharlotteGel74-2, 74-3Gel extractionPP46Lambda-Lys_pSBX_pSBXH2O30.0530.043 echte konz.
349
P75-2P7508-0817:45CharlotteGel74-4, 74-5Gel extractionPP75NpuDnaEC_T7_RBS-BsaI_mRFPH2O30.01528.063.0
350
P76-2P7608-0817:45CharlotteGel74-6, 74-7Gel extractionPP76NpuDnaEN_mRFP_BsaI-taataa_iGEMHDH2O30.08030.0139.5
351
P61-3P6108-0817:45CharlotteGel74-8, 74-9Gel extractionPP61pSBX4A5_taataa_atgH2O30.05830.057.5
352
P61-4P6108-0817:45CharlotteGel74-8, 74-9Gel extractionPP61pSBX4A5_taataa_atgH2O30.0530.020.0
353
P73-2P7308-0817:45CharlotteGel74-10, 74-11Gel extractionPP73pSBX1T3_taataa_atgH2O30.03030.030.0
354
P74-2P7408-0817:45CharlotteGel74-12, 74-13Gel extractionPP74pSBX4C5_taataa_atgH2O30.02730.027.0
355
O58-7O5808-0817:45AnnaGrowth383C3
Qiagen miniprep kit
Plasmid5858pSBX1C3-BBa_I13507H2O00.0O58-4
356
P44-3P4408-0819:00JakobGel82-2-1, 82-3-1Gel extractionPP44ccdB_BsmBI_BsmBIH2O30.010930.0
357
P45-3P4508-0920:00JakobGel82-4-1, 82-5-1Gel extractionPP45mRFP_BsaI_BsaIH2O30.02430.0
358
P48-3P4808-1021:00JakobGel82-6-1, 82-7-1Gel extractionPP48NpuDnaEN_H2O30.09130.0
359
P49-3P4908-1122:00JakobGel82-8-1, 82-9-1Gel extractionPP49NpuDnaEC_H2O30.06230.0
360
P45-4P4508-091:30JakobGel83-3-1, 83-4-1Gel extractionPP45mRFP_BsaI_BsaIH2O30.05530.0
361
P69-2P6908-093:50JakobGel84-2-1, 84-3-1Gel extractionPP69pSBX1T3_ccdB-BsmBI_BsaI-mRFPH2O30.04130.0
362
P70-2P7008-093:50JakobGel84-4-1, 84-5-1Gel extractionPP70pSBX1T3_mRFP-BsaI_BsmBI-ccdBH2O30.03530.0
363
P59-3P5908-093:50JakobGel84-6-1, 84-7-1Gel extractionPP59pSBX4A5_ccdB-BsmBI_BsaI-mRFPH2O30.01930.0
364
P60-2P6008-093:50JakobGel84-8-1, 84-9-1Gel extractionPP60pSBX4A5_mRFP-BsaI_BsmBI-ccdBH2O30.02130.0
365
P71-2P7108-093:50JakobGel84-10-1, 84-11-1Gel extractionPP71pSBX4C5_ccdB-BsmBI_BsaI-mRFP_NH2O30.03030.0
366
P72-2P7208-093:50JakobGel84-12-1, 84-13-1Gel extractionPP72pSBX4C5_mRFP-BsaI_BsmBI-ccdB_CH2O30.01530.0
367
O90-5O9008-0903:54JanPurificationO90DilutionPlasmid9090pSB1C3-BBa_J06702H2O77.0155.0
368
P97P9708-0922:44CaroGel76-1-2Gel extractionPP97mRFPn_1-154H2O30.013830.0
369
P98P9808-0922:44CaroGel76-3-4Gel extractionPP98mRFP_1-168H2O30.016430.0
370
P99P9908-0922:44CaroGel76-6-7Gel extractionPP99mRFPc_155-225H2O30.012430.0
371
P100P10008-0922:44CaroGel76-8, 77-1Gel extractionPP100mRFPc_169-225H2O30.016230.0
372
P101P10108-0922:44CaroGel77-3-4Gel extractionPP101RenLucN_1-229_H6_GATG_BsaI_KGG_TGCT_BsaIH2O30.015830.0
373
P102P10208-0922:44CaroGel77-5-6Gel extractionPP102RenLucC_230-311_NWC_CAAC_BsaI_H6_TAAT_BsaIH2O30.017330.0
374
P103P10308-0922:44CaroGel77-7-8Gel extractionPP103FireLucN_1-437_H6_GATG_BsaI_KGG_TGCT_BsaIH2O30.09930.0
375
P104P10408-0922:44CaroGel78-1-2Gel extractionPP104FireLucC_438-550_NWC_CAAC_BsaI_H6_TAAT_BsaIH2O30.015330.0
376
P105P10508-0922:44CaroGel78-3-5Gel extractionPP105mCherryN_1-168_H6_GATG_BsaI_KGG_TGCT_BsaIH2O30.05130.0
377
P106P10608-0922:44CaroGel78-6-7Gel extractionPP106mCherryC_169-236_NWC_CAAC_BsaI_H6_TAAT_BsaIH2O30.012630.0
378
P107P10708-0922:44CaroGel78-8, 79-1Gel extractionPP107GFPn_1-64_H6_BsaIH2O30.010430.0
379
P108P10808-0922:44CaroGel79-2-3Gel extractionPP108GFPn_1-157_H6_BsaIH2O30.011530.0
380
P109P10908-0922:44CaroGel79-5-6Gel extractionPP109GFPc_65-238_NWC_CAAC_BsaI_H6_TAAT_BsaIH2O30.016130.0
381
P110P110today22:44CaroGel79-7-8Gel extractionPP110GFPc_158-238_NWC_CAAC_BsaI_H6_TAAT_BsaIH2O30.010330.0
382
P79P7908-0911:15JakobGel90-2-1, 90-3-1Gel extractionPP79mRFP-NpuDnaEN-ccdB_BsaI_BsmBIH2O30.026-11.6
383
P81P8108-0912:15JakobGel90-4-1, 90-5-1Gel extractionPP81ccdB-NpuDnaEC-mRFP_BsmBI_BsaIH2O30.047-2.5
384
P112P112
s/cmQMwHQNwJDdmZQFy
08-0712:00JakobExtern
from Dominik Niopek
PP112NEBGibsonPositiveControl0.0
385
P59-4P5908-1011:00CaroGel94-2-1, 94-3-1Gel extractionPP59pSBX4A5_ccdB-BsmBI_BsaI-mRFPH2O30.030-3.3
386
P60-3P6008-1011:00CaroGel94-4-1, 94-5-1Gel extractionPP60pSBX4A5_mRFP-BsaI_BsmBI-ccdBH2O30.030-3.3
387
O80-3O80today18:00
Constantin
Growth300C4
Qiagen miniprep kit
Plasmid8080pSBX4C5-2ZSspH6:GFPH2O30.0030.0148O80-2FG1203, FG1208ok, ok
388
O81-2O81today18:00
Constantin
Growth301C3
Qiagen miniprep kit
Plasmid8181pSBX4C5-2ZNpuH6:GFPH2O30.031130.0149O81FG1204, FG1209pSBX4C5-2ZSspH6:GFP, pSBX4C5-2ZSspH6:GFP
389
O86O86today18:00
Constantin
Growth372C4
Qiagen miniprep kit
Plasmid8686pSB1AK8-BBa_I712074-2ZSspH6:GFPH2O30.011230.0150discardedS86FG1205, FG1210is just pSB1AK8-BBa_I712074, is just pSB1AK8-BBa_I712074
390
O87O87today18:00
Constantin
Growth351C7
Qiagen miniprep kit
Plasmid8787pSB1AK8-BBa_I712074-2ZNpuH6:GFPH2O30.018030.0151discardedS87FG1206, FG1211mixture of pSB1AK8-T7-2ZNpu:(GFP-H6) and unknown, mixture of pSB1AK8-T7-2ZNpu:(GFP-H6) and unknown
391
O48-2O48today18:00
Constantin
Growth263C1-4
Qiagen miniprep kit
Plasmid4848pSB1AK8-T7-2ZNpu:(GFP-H6)H2O30.09630.0O48FG1207, FG1212ok, ok
392
P62P6208-1022:00
Constantin
Gel99-2-1Gel extractionPP62pSBX1K3_taataa_atgH2O30.03330.0
399
P79-2P7908-1111:40JakobGel107-2-1, 107-3-1Gel extractionPP79mRFP-NpuDnaEN-ccdB_BsaI_BsmBIH2O30.0828.7
400
P81-2P8108-1111:40JakobGel107-4-1, 107-5-1Gel extractionPP81ccdB-NpuDnaEC-mRFP_BsmBI_BsaIH2O30.02229.5
401
P46-4P4608-113:00CharlotteGel103-2, 103-3Gel extractionPP46Lambda-Lys_pSBX_pSBXH2O30.0830.0estimated
402
O102O10208-1117:30JanGel105-3-1Gel extractionPlasmid102102pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3H2O10.010.0
403
O102-2O10208-1117:34JanAssemblyA102
PCR purification kit
Plasmid102102pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3H2O10.00
404
P79-3P7908-1111:00JakobPurificationP79-2DilutionPP79mRFP-NpuDnaEN-ccdB_BsaI_BsmBIH2O100.00100.0
405
P81-3P8108-1108-11JakobPurificationP81-2DilutionPP81ccdB-NpuDnaEC-mRFP_BsmBI_BsaIH2O100.00100.0
406
P122P12208-121:30MaxWGel110-2, 110-3Gel extractionPP1222ZNpu_pSBX_taataa_pSBXH2O30.012530.0
407
O65-11O6508-1213:00CharlottePurificationO65-7DilutionPlasmid6565pSB1C3-BBa_J04450H2O86.0123.8
408
P45-5P4508-1217:50JakobGel112-4-1Gel extractionPP45mRFP_BsaI_BsaIH2O30.09330.0
409
O98O9808-1221:43MaxWAssemblyA98#2
PCR purification kit
Plasmid9898pSBX4C5-2Zv2NpuH2O0.0discarded
410
O98-2O9808-1221:43MaxWAssemblyA98#3
PCR purification kit
Plasmid9898pSBX4C5-2Zv2NpuH2O0.0discarded
411
O102-3O10208-133:10MaxWGrowth423C2
Qiagen miniprep kit
Plasmid102102pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3H2O50.07650.0152O102-2FG1200, II2274ok, ok
412
O102-4O10208-133:10MaxWGrowth423C3
Qiagen miniprep kit
Plasmid102102pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3H2O50.07050.0153O102-2FG1201, II2275ok, ok
413
O102-5O10208-133:10MaxWGrowth423C4
Qiagen miniprep kit
Plasmid102102pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3H2O50.06150.0154discardedO102-2
414
O102-6O10208-133:10MaxWGrowth424C1
Qiagen miniprep kit
Plasmid102102pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3H2O50.06650.0155A102
415
O102-7O10208-133:10MaxWGrowth424C2
Qiagen miniprep kit
Plasmid102102pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3H2O50.08243.9156A102FG1202, II2276ok, ok
416
O103O10308-1417:44MaxWGrowth433C1
Qiagen miniprep kit
Plasmid103103pSBX1K3-2Zv2NpuH2O30.0662.6157charlies zeug........A103FG1213, FG1214wrong, charlie's stuff, wrong, charlie's stuff
417
O104O10408-1417:44MaxWGrowth434C1
Qiagen miniprep kit
Plasmid104104pSB1C3-BBa_I746908H2O30.09830.0158D2014-4-5CII2277, II2278ok, ok
418
O13-3O1308-1421:30JakobPurificationO13DilutionPlasmid1313pSB1C3-pKH6insertH2O102.0198.0
419
O29-3O2908-1421:30JakobPurificationO29DilutionPlasmid2929pVS41H2O114.0198.0
420
O30-3O3008-1421:30JakobPurificationO30DilutionPlasmid3030pVS07H2O151.61145.6
421
P134P13408-1422:00MaxWGel121-2, 121-3Gel extractionPP134LambdaLys_BsaI_c-ext_HISTAG_n-ext_BsaIH2O40.06340.0
422
P135P13508-1422:00MaxWGel121-4, 121-5Gel extractionPP135LambdaLys_BsaI_c-ext_RIGIDLINKER_n-ext_BsaIH2O40.06440.0
423
O103-2O10308-1422:26MaxWGrowth433C4
Qiagen miniprep kit
Plasmid103103pSBX1K3-2Zv2NpuH2O30.09317.8159restriktion sagt: passtA103II2279, II2280ok (silent mutation in selection marker), ok (silent mutation in selection marker)
424
P44-4P4408-15O98JakobGel122-2-1, 122-3-1Gel extractionPP44ccdB_BsmBI_BsmBIH2O30.012130.0
425
P45-6P4508-1508-15JakobGel122-4-1, 122-5-1Gel extractionPP45mRFP_BsaI_BsaIH2O30.07030.0
426
P48-4P4808-1508-15JakobGel122-6-1, 122-7-1Gel extractionPP48NpuDnaEN_H2O30.05230.0
427
P49-4P4908-1508-15JakobGel122-8-1, 122-9-1Gel extractionPP49NpuDnaEC_H2O30.04230.0
428
R127R12708-1501:32MaxWRestriction127
PCR purification kit
Restriction
127pSBX4C5-T7RBS-IGEMHD_v3 (E/P)H2O40.04039.4
429
R129R12908-1502:24MaxWGel123-4-1, 123-5-1Gel extraction
Restriction
129pSBX1K3-2Zv2Npu (E/P)H2O30.0317.9
430
P59-5P5908-159:15JakobGel124-2-1, 124-3-1Gel extractionPP59pSBX4A5_ccdB-BsmBI_BsaI-mRFPH2O30.010530.0
431
P60-4P6008-159:15JakobGel124-4-1, 124-5-1Gel extractionPP60pSBX4A5_mRFP-BsaI_BsmBI-ccdBH2O30.08730.0
432
P69-3P6908-159:15JakobGel124-6-1, 124-7-1Gel extractionPP69pSBX1T3_ccdB-BsmBI_BsaI-mRFPH2O30.05330.0
433
P70-3P7008-159:15JakobGel124-8-1, 124-9-1Gel extractionPP70pSBX1T3_mRFP-BsaI_BsmBI-ccdBH2O30.04430.0
434
P138P13808-1523:32ConstiGel125-2,125-3Gel extractionPP138Xyla_BB_RBS34_X_SPH2O30.0330.0
435
P138-2P13808-1523:32ConstiGel125-4,125-5Gel extractionPP138Xyla_BB_RBS34_X_SPH2O30.067-1.0
436
P139P13908-1523:32ConstiGel125-6,125-7Gel extractionPP139Xyla_BsaI_circ_HisH2O30.0730.0
437
P139-2P13908-1523:32ConstiGel125-8,125-9Gel extractionPP139Xyla_BsaI_circ_HisH2O30.02030.0
438
P140P14008-1523:32ConstiGel125-11,125-12Gel extractionPP140Xyla_circ_noHisH2O30.0730.0
439
P140-2P14008-1523:32ConstiGel125-14,125-14Gel extractionPP140Xyla_circ_noHisH2O30.01330.0
442
P141-3P14108-1523:32CaroGel126-2,126-3Gel extractionPP141Xyla_failed_circ_HisH2O30.0030.0
443
P36-3P3607-2020:30MaggieGel
34-1-2.2kb,34-3-2.2kb
Gel extractionPP36pSB1A3_SortAc-H6_Smt30.0
444
P35-3P3507-2020:30MaggieGel
34-4-778bp,34-5-778bp
Gel extractionPP35ccdB_Smt3-TEVc-BsaI_SortAc-H6-RFC10suffix0.0
445
P34-3P3407-2020:30MaggieGel
34-6-342bp,34-7-342bp
Gel extractionPP34Smt3_RFC10prefix_TEVc-BsaI0.0
446
R130R13008-1621:24MaxWGel129-2, 129-3Gel extraction
Restriction
130pSBX1T3-BBa_I13507 (X/P)H2O30.0720.1
447
R132R13208-1621:24MaxWGel129-6, 129-7Gel extraction
Restriction
132pSBX4C5-BBa_I13507 (X/P)H2O30.0919.7
448
R133R13308-1621:24MaxWGel131-2, 131-3Gel extraction
Restriction
133pSBX4A5-BBa_I13507 (X/P)H2O30.0930.0
449
R134R13408-1621:24MaxWGel131-4, 131-5Gel extraction
Restriction
134Xyla_BB_RBS34_X_SP (X/P)H2O30.0715.7
450
R136R13608-164:10SilvanRestriction136
PCR purification kit
Restriction
136pSBX1K3-T7RBS_BBv2 (S/P)EB30.028.0
451
R137R13708-164:10SilvanRestriction137
PCR purification kit
Restriction
137pSBX1K3-T7RBS_BBv2 (S/P)EB30.028.0
452
R138R13808-164:10SilvanRestriction138
PCR purification kit
Restriction
138pSBX1K3-T7RBS_BBv2 (S/P)EB30.028.0
453
P79-4P7908-1717:15JakobGel136-2-1, 136-3-1Gel extractionPP79mRFP-NpuDnaEN-ccdB_BsaI_BsmBIH2O30.04530.0
454
P81-4P8108-1818:15JakobGel136-4-1, 136-5-1Gel extractionPP81ccdB-NpuDnaEC-mRFP_BsmBI_BsaIH2O30.04630.0
455
O98-3O9808-1723:46CaroGrowth438C8
Qiagen miniprep kit
Plasmid9898pSBX4C5-2Zv2NpuH2O30.03570164discardedS98
456
O98-4O9808-1723:46CaroGrowth438C9
Qiagen miniprep kit
Plasmid9898pSBX4C5-2Zv2NpuH2O30.043919.8165S98II2273ok
457
O103-3O10308-1708-17CaroGrowth443C1
Qiagen miniprep kit
Plasmid103103pSBX1K3-2Zv2NpuH2O30.016210.5168A103#2
458
O110O11008-1708-17CaroGrowth444C1
Qiagen miniprep kit
Plasmid110110pSBX1K3-2Zv2Npu:(LambdaLys-H6)H2O30.03250169discardedG110
459
O111O11108-1708-17CaroGrowth445C1
Qiagen miniprep kit
Plasmid111111pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)H2O30.032329.0170discardedG111
460
O112O11208-1708-17CaroGrowth446C2
Qiagen miniprep kit
Plasmid112112pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_HisH2O30.02290171discardedG112
461
O113O11308-1708-17CaroGrowth447C1
Qiagen miniprep kit
Plasmid113113pSBX1K3-2Zv2Npu:Xyla_circ_noHisH2O30.02170172discardedG113
462
O109O10908-1815:30LisaGrowth458C1
Qiagen miniprep kit
Plasmid109109pSB1C3-BBa_K808000H2O30.037524.7179D2014-2-7EII2288ok
463
O109-2O10908-1815:30LisaGrowth458C2
Qiagen miniprep kit
Plasmid109109pSB1C3-BBa_K808000H2O30.01220180discardedD2014-2-7E
464
O109-3O10908-1815:30LisaGrowth458C3
Qiagen miniprep kit
Plasmid109109pSB1C3-BBa_K808000H2O30.03340181discardedD2014-2-7E
465
O117O11708-1815:30LisaGrowth467C1
Qiagen miniprep kit
Plasmid117117pSB1C3-BBa_K741002H2O30.02240182discarded
D2013-2-17M
466
O117-2O11708-1815:30LisaGrowth467C2
Qiagen miniprep kit
Plasmid117117pSB1C3-BBa_K741002H2O30.025922.3183
D2013-2-17M
II2289ok
467
O117-3O11708-1815:30LisaGrowth467C3
Qiagen miniprep kit
Plasmid117117pSB1C3-BBa_K741002H2O30.016930.0184discarded
D2013-2-17M
468
P141-4P14108-18ConstiPurificationP141-3DilutionPP141Xyla_failed_circ_HisH2O10.010.0
1:10 Dilution of P141-3 because conc. could be too high for another PCR
469
O103-4O10308-16MaxWGrowth433C4-1
Qiagen miniprep kit
Plasmid103103pSBX1K3-2Zv2NpuH2O50.08750.0
reprep of O103-2 (pos sequenzing)
A103
470
O55-5O5508-1815:45SilvanGrowth448C1
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)338-2.0106S38II2281
471
O55-6O5508-1808-18SilvanGrowth448C2
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)154-2.0107S38II2282
472
O55-7O5508-1816:00SilvanGrowth448C3
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)132-2.0173S38II2283
473
O55-8O5508-1816:00SilvanGrowth448C4
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)162-2.0174S38II2284
474
O116O11608-1816:00SilvanGrowth453C3
Qiagen miniprep kit
Plasmid116116pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB)223-2.0175discardedS116II2285
475
O116-2O11608-1816:00SilvanGrowth454C1
Qiagen miniprep kit
Plasmid116116pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB)190-2.0176discardedS116#2II2286single base deletion
476
O116-3O11608-1816:00SilvanGrowth455C1
Qiagen miniprep kit
Plasmid116116pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB)243-2.0177discardedS116#3II2287two-base deletion and point mutation
477
O116-4O11608-1816:00SilvanGrowth455C3
Qiagen miniprep kit
Plasmid116116pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB)38-2.0178discardedS116#3
478
R140R14008-1818:00CharlotteRestriction140
PCR purification kit
Restriction
140pSB1AK8-BBa_I712074 (S/P)H2O50.01647.4
479
R141R14108-1818:00CharlotteGel140-2-2, 140-3-2Gel extraction
Restriction
141pSBX4C5-2ZSspH6:GFP (X/P)H2O50.01145.8
480
P141-5P14108-1818:33CaroPCR479
PCR purification kit
PP141Xyla_failed_circ_HisH2O30.030.0
481
P141-6P14108-1818:37CaroPCR480
PCR purification kit
PP141Xyla_failed_circ_HisH2O30.030.0
482
P141-7P14108-1819:11ConstiGel142-3, 142-4Gel extractionPP141Xyla_failed_circ_HisH2O30.03030.0
483
P138-3P13808-1821:18CaroPCR482
PCR purification kit
PP138Xyla_BB_RBS34_X_SPH2O30.015.0
484
R145R14508-1821:23CaroRestriction145
PCR purification kit
Restriction
145Xyla_BB_RBS34_X_SP (X/P)H2O30.030.0
485
R139R13908-1821:44CaroRestriction139Gel extraction
Restriction
139pSBX1T3-BBa_I13507 (X/P)H2O30.0516.2
486
R142R14208-1821:44CaroRestriction142Gel extraction
Restriction
142pSBX4A5-BBa_I13507 (X/P)H2O30.01419.4
487
R143R14308-1821:44CaroRestriction143Gel extraction
Restriction
143pSBX4C5-BBa_I13507 (X/P)H2O30.01218.9
488
R144R14408-1821:44CaroRestriction144Gel extraction
Restriction
144pSBX1K3-BBa_I13507 (X/P)H2O30.030.0
489
R145-2R14508-1821:44CaroRestriction145Gel extraction
Restriction
145Xyla_BB_RBS34_X_SP (X/P)H2O30.0519.2
490
P39P3908-181:00SilvanGel145-2-x,145-3-xGel extractionPP39ccdB_pSB*3-BsaI_SortAc-His6EB30.017230.0
491
P148P14808-181:00SilvanGel145-4-x,145-5-xGel extractionPP148mRFPexp_BsaI=CGCA=BsmBI_BsmBI=CTTC=BsaIEB30.012430.0
492
P147P14708-181:00SilvanGel145-8-x,145-9-xGel extractionPP147mDNMT1(731-1602)_BsaI=GGGT-GGG_CGCA=BsaIEB30.0301.9
493
P151P15108-1921:20LisaGel147-4, 147-5Gel extractionPP151pSBX1K3-T7RBS_taataa-iGEMHD_BsaI-mRFPH2O30.08228.0
494
P152-008-2010:00LisaGel151-2, 151-3Gel extractionPP152pSBX1K3-T7RBS_mRFP-BsaI_atgH2O30.05528.0
495
P153P15308-1923:45CaroGel
148-2,148-3,148-5,148-6,148-10,148-11
Gel extractionPP153mRFP-NpuDnaEN-ccdB_X_SPH2O30.0373.0
496
P154P15408-1923:45CaroGel
148-4,148-5,148-8,148-9,148-12,148-13
Gel extractionPP154ccdB-NpuDnaEC-mRFP_X_SPH2O30.05110.4
497
R151R15108-2001:26CaroRestriction151
PCR purification kit
Restriction
151mRFP-NpuDnaEN-ccdB_X_SP (X/P)H2O30.0304.2
498
R152R15208-2001:26CaroRestriction152
PCR purification kit
Restriction
152ccdB-NpuDnaEC-mRFP_X_SP (X/P)H2O30.04310.5
499
R157R15708-20CharlotteGel153-4, 154-2, 154-3Gel extraction
Restriction
157pSBX4C5-2ZSspH6:GFP (X/P)H2O30.01321.7
500
R156R15608-20CharlotteGel153-2, 153-3Gel extraction
Restriction
156pSB1AK8-BBa_I712074 (S/P)H2O30.01527.7
501
R158R15808-20CharlotteRestriction158
PCR purification kit
Restriction
158pSB1AK8-BBa_I712074 (S/P)H2O30.0425.4
502
R158-2R15808-20CharlotteRestriction158
PCR purification kit
Restriction
158pSB1AK8-BBa_I712074 (S/P)H2O30.0325.0phosphatase was added
503
R160R16008-2017:06CaroGel155-2Gel extraction
Restriction
160pSBX1T3-BBa_I13507 (X/P)H2O30.095.8
504
R162R16208-2017:06CaroGel155-4Gel extraction
Restriction
162pSBX4C5-BBa_I13507 (X/P)H2O30.0136.9
505
O55-9O5508-2018:20SilvanGrowth211C5
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)EB50.025938.2A55
76DB25, 76DB27, 76DB63
ok, bad sequencing, ok
506
O55-10O5508-2018:20SilvanGrowth212C6
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)EB50.022648.0A55#276DB26mixture
507
P153-2P15308-2019:00JakobGel158-2-1, 158-3-1Gel extractionPP153mRFP-NpuDnaEN-ccdB_X_SPH2O30.03113.7
508
P154-2P15408-2019:00JakobGel158-4-1, 158-5-1Gel extractionPP154ccdB-NpuDnaEC-mRFP_X_SPH2O30.02912.7
509
O94O9408-2021:09CaroLigationS94#2
PCR purification kit
Plasmid9494pSBX4C5-2GateNpuDnaE(N)H2O30.00
510
O92O9208-2021:09CaroLigationS92#2
PCR purification kit
Plasmid9292pSBX1T3-2GateNpuDnaE(N)H2O30.00
511
O95O9508-2021:09CaroLigationS95#2
PCR purification kit
Plasmid9595pSBX4C5-2GateNpuDnaE(C)H2O30.00
512
O93O9308-2021:09CaroLigationS93#2
PCR purification kit
Plasmid9393pSBX1T3-2GateNpuDnaE(C)H2O30.00
513
O94-2O9408-2021:09CaroLigationS94#3
PCR purification kit
Plasmid9494pSBX4C5-2GateNpuDnaE(N)H2O30.00
514
O92-2O9208-2021:09CaroLigationS92#3
PCR purification kit
Plasmid9292pSBX1T3-2GateNpuDnaE(N)H2O30.00
515
O95-2O9508-2021:09CaroLigationS95#3
PCR purification kit
Plasmid9595pSBX4C5-2GateNpuDnaE(C)H2O30.00
516
O93-2O9308-2021:09CaroLigationS93#3
PCR purification kit
Plasmid9393pSBX1T3-2GateNpuDnaE(C)H2O30.00
517
O104-2O10408-2022:11JanPurificationO104DilutionPlasmid104104pSB1C3-BBa_I746908H2O97.0191.0
518
R166R16608-2023:30JakobRestriction166
PCR purification kit
Restriction
166mRFP-NpuDnaEN-ccdB_X_SP (X/P)H2O30.05-6.7dont believe it
519
R167R16708-2023:30JakobRestriction167
PCR purification kit
Restriction
167ccdB-NpuDnaEC-mRFP_X_SP (X/P)H2O30.06-5.0dont believe it
520
P132P13208-2104:58JanGel159-6-1,159-7-1Gel extractionPP132sfGFP^N_T7RBS-H6_C->G-NpuDnaE^NH2O30.08230.0
521
P129P12908-2104:58JanGel159-8-1,159-9-1Gel extractionPP129sfGFP^N_T7RBS-H6_NpuDnaE^NH2O30.06330.0
522
P125P12508-2104:58JanGel160-2-1,160-3-1Gel extractionPP125sfGFP^C_NpuDnaE^C_H6H2O30.023030.0
523
P130P13008-2104:58JanGel160-4-1,160-5-1Gel extractionPP130pSBX1K3-T7RBS_H6-NpuDnaE^N_H6H2O30.06630.0
524
P126P12608-2104:58JanGel160-6-1,160-7-1Gel extractionPP126pSB_H6-sfGFP^C_NpuDnaE^C-H6H2O30.07130.0
525
O27-2O2708-2115:30LisaPurificationO27DilutionPlasmid2727pCL20H2O40.0136.0
526
O110-2O11008-2116:30MaxWGrowth476C1
Qiagen miniprep kit
Plasmid110110pSBX1K3-2Zv2Npu:(LambdaLys-H6)H2O50.03200196discardedG110#276DB28several deletions
527
O110-3O11008-2116:30MaxWGrowth476C2
Qiagen miniprep kit
Plasmid110110pSBX1K3-2Zv2Npu:(LambdaLys-H6)H2O50.02810197discardedG110#276DB77, 76DB82a deletion, a deletion
528
R153R15308-2118:00SilvanGel
163-1-100bp, 163-2-100bp, 163-3-100bp
Gel extraction
Restriction
153pSB1A3-Smt3-2ZSortA-H6 (ccdB) (X/BglII)EB25.0119.4
measured 9.7 (impossible)
529
R154R15408-2118:00SilvanGel
163-5-x, 163-7-x, 163-8-x
Gel extraction
Restriction
154pSB1A3-Smt3-2ZSortA-H6 (ccdB) (BglII/P)EB25.01019.9
530
P158P15808-2119:40LisaGel158Gel extractionPP158SspDnaBC_M+H6_N->A+SGGH2O30.0856.4
531
P159P15908-2119:40LisaGel159Gel extractionPP159SspDnaBC_M-noH6_N->A+SGGH2O30.0835.8
532
P124P12408-2120:00JanGel164-2-1,164-3-1Gel extractionPP124NpuDnaE^C_H6_sfGFPH2O20.00
lower band, discarded and redone
533
P127P12708-2120:00JanGel164-4-1,164-5-1Gel extractionPP127NpuDnaE^C SN->AG_H6_sfGFPH2O20.00
lower band, discarded and redone
534
P124-2P12408-2120:00JanGel164-2-2,164-3-2Gel extractionPP124NpuDnaE^C_H6_sfGFPH2O20.00
upper band, discarded and redone
535
P127-2P12708-2120:00JanGel164-4-2,164-5-2Gel extractionPP127NpuDnaE^C SN->AG_H6_sfGFPH2O20.00
upper band, discarded and redone
536
O30-4O3008-2120:26JanPurificationO30DilutionPlasmid3030pVS07H20150.01130.0
537
R168R16808-221:00JakobGel168-2-1, 168-3-1Gel extraction
Restriction
168pSBX1K3-BBa_I13507 (X/P)H2O30.01126.6
538
R169R16908-221:00JakobGel168-4-1, 168-5-1Gel extraction
Restriction
169pSBX1T3-BBa_I13507 (X/P)H2O30.01126.4
539
R170R17008-221:00JakobGel168-6-1, 168-7-1Gel extraction
Restriction
170pSBX4A5-BBa_I13507 (X/P)H2O30.0924.6
540
R171R17108-221:00JakobGel168-9-1, 168-10-1Gel extraction
Restriction
171pSBX4C5-BBa_I13507 (X/P)H2O30.01326.2
541
O122O12208-2211:00LisaGrowth485C1
Qiagen miniprep kit
Plasmid122122pSBX1K3-1GateNpuDnaE(N)H2O30.030229.3199with igemhdA122
542
O123O12308-2211:00LisaGrowth486C1
Qiagen miniprep kit
Plasmid123123pSBX1K3-1GateNpuDnaE(C)H2O30.031029.4200with igemhdA123II2290, II2291mutation A220G K74E in the mRFP CDS, besides ok, mutation A220G K74E in the mRFP CDS, besides ok
543
O123-2O12308-2211:00LisaGrowth486C2
Qiagen miniprep kit
Plasmid123123pSBX1K3-1GateNpuDnaE(C)H2O30.016528.8201with igemhdA123
544
O86-2O8608-2212:00CharlotteGrowth508C2
Qiagen miniprep kit
Plasmid8686pSB1AK8-BBa_I712074-2ZSspH6:GFPH2O50.050.0S86#4
545
O117-4O11708-2220:40LisaPurificationO117-2DilutionPlasmid117117pSB1C3-BBa_K741002H2O100.0391.0
546
P128P12808-2318:28JanGel173-1-1,173-2-1Gel extractionPP128NpuDnaE^N_sfGFP^N_H6H2O30.07528.0
547
P131P13108-2318:28JanGel173-3-1,173-4-1Gel extractionPP131C->G-NpuDnaE^N_sfGFP_H6H2O30.09828.0
548
P40P4008-2318:28JanGel178-1-1Gel extractionPP40pSB1C3_ccdB_SortAc-His6H2O30.04528.0
549
O29-4O2908-2318:28JanPurificationO29DilutionPlasmid2929pVS41H2O114.0168.0
550
P124-3P12408-2323:21JanPCR658
PCR purification kit
PP124NpuDnaE^C_H6_sfGFPH2O20.05818.0
551
P127-3P12708-2323:21JanPCR660
PCR purification kit
PP127NpuDnaE^C SN->AG_H6_sfGFPH2O20.08318.0
552
O111-2O11108-240:56MaxWGrowth501C3
Qiagen miniprep kit
Plasmid111111pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)H2O50.08150.0204G111#2II2294, II2295EINE, 1, EINE fucking mutation........., EINE, 1, EINE fucking mutation.........
553
O113-2O11308-240:56MaxWGrowth503C5
Qiagen miniprep kit
Plasmid113113pSBX1K3-2Zv2Npu:Xyla_circ_noHisH2O50.02990205discardedG113#2II2298, II2299fürn arsch, fürn arsch
554
O112-2O11208-240:56MaxWGrowth502C6
Qiagen miniprep kit
Plasmid112112pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_HisH2O50.034948.6206G112#2II2296, II2297ok, ok
555
O112-3O11208-240:56MaxWGrowth502C7
Qiagen miniprep kit
Plasmid112112pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_HisH2O50.02640207discardedG112#2
556
O112-4O11208-240:56MaxWGrowth502C8
Qiagen miniprep kit
Plasmid112112pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_HisH2O50.02930208discardedG112#2
557
O124O12408-240:56MaxWGrowth504C6
Qiagen miniprep kit
Plasmid124124pSBX1K3-2Zv2Npu:Xyla(H6)destrCextH2O50.027246.0209G12476DB73, 76DB78insertions, insertions
558
O124-2O12408-240:56MaxWGrowth504C7
Qiagen miniprep kit
Plasmid124124pSBX1K3-2Zv2Npu:Xyla(H6)destrCextH2O50.028650.0210G124II2300, II2301fürn arsch, fürn arsch
559
O124-3O12408-240:56MaxWGrowth504C8
Qiagen miniprep kit
Plasmid124124pSBX1K3-2Zv2Npu:Xyla(H6)destrCextH2O50.023743.9211G12476DB74, 76DB79ok, ok
560
O124-4O12408-240:56MaxWGrowth504C9
Qiagen miniprep kit
Plasmid124124pSBX1K3-2Zv2Npu:Xyla(H6)destrCextH2O50.026650.0212G124
561
O125O12508-240:56MaxWGrowth512C5
Qiagen miniprep kit
Plasmid125125pSB1C3-Smt3-2ZSortA-H6 (ccdB)H2O50.025046.0213L12576DB29, 76DB30negative, contains GFP, negative, contains GFP
562
O125-2O12508-240:56MaxWGrowth512C6
Qiagen miniprep kit
Plasmid125125pSB1C3-Smt3-2ZSortA-H6 (ccdB)H2O50.027147.0214L12576DB31, 76DB32negative, contains GFP, negative, contains GFP
563
O125-3O12508-240:56MaxWGrowth512C7
Qiagen miniprep kit
Plasmid125125pSB1C3-Smt3-2ZSortA-H6 (ccdB)H2O50.037547.6215L12576DB33, 76DB34negative, contains GFP, negative, contains GFP
564
O94-3O9408-241:30JakobGrowth525C1
Qiagen miniprep kit
Plasmid9494pSBX4C5-2GateNpuDnaE(N)H2O30.030629.0S94#4II2302, II2306bad sequencing, but probaBIy ok, bad sequencing, but probaBIy ok
565
O94-4O9408-241:30JakobGrowth525C2
Qiagen miniprep kit
Plasmid9494pSBX4C5-2GateNpuDnaE(N)H2O30.02500discardedS94#4II2303, II2307bad sequencing, but probaBIy ok, bad sequencing, but probaBIy ok
566
O94-5O9408-241:30JakobGrowth525C3
Qiagen miniprep kit
Plasmid9494pSBX4C5-2GateNpuDnaE(N)H2O30.03110discardedS94#4
567
O94-6O9408-241:30JakobGrowth525C4
Qiagen miniprep kit
Plasmid9494pSBX4C5-2GateNpuDnaE(N)H2O30.03530discardedS94#4
568
O94-7O9408-241:30JakobGrowth525C5
Qiagen miniprep kit
Plasmid9494pSBX4C5-2GateNpuDnaE(N)H2O30.03430discardedS94#4
569
O95-3O9508-241:30JakobGrowth526C1
Qiagen miniprep kit
Plasmid9595pSBX4C5-2GateNpuDnaE(C)H2O30.027829.0S95#4II2304, II2308bad sequencing, but probaBIy ok, bad sequencing, but probaBIy ok
570
O95-4O9508-241:30JakobGrowth526C2
Qiagen miniprep kit
Plasmid9595pSBX4C5-2GateNpuDnaE(C)H2O30.03060discardedS95#4II2305, II2309bad sequencing, but probaBIy ok, bad sequencing, but probaBIy ok
571
O95-5O9508-241:30JakobGrowth526C3
Qiagen miniprep kit
Plasmid9595pSBX4C5-2GateNpuDnaE(C)H2O30.03590discardedS95#4
572
O95-6O9508-241:30JakobGrowth526C4
Qiagen miniprep kit
Plasmid9595pSBX4C5-2GateNpuDnaE(C)H2O30.02120discardedS95#4
573
O95-7O9508-241:30JakobGrowth526C5
Qiagen miniprep kit
Plasmid9595pSBX4C5-2GateNpuDnaE(C)H2O30.03090discardedS95#4
574
P152P15208-2422:20MaxWGel186-2Gel extractionPP152pSBX1K3-T7RBS_mRFP-BsaI_atgH2O30.04830.0
575
P166P16608-251:16JanGel192-1-1,192-2-1Gel extractionPP166sfGFP^N_T7RBS-H6_H6H2O20.017218.0
576
P167P16708-251:16JanGel192-3-1,192-4-1Gel extractionPP167pSBX1K3-T7RBS_sfGFP^C-H6_H6H2O20.08818.0
577
O86-3O8608-251:42CharlotteGrowth510C2
Qiagen miniprep kit
Plasmid8686pSB1AK8-BBa_I712074-2ZSspH6:GFPH2O30.036030.0218for sequencingS86#5II2310, II2311ok, ok
578
O105O10508-2518:00CaroGrowth527C2
Qiagen miniprep kit
Plasmid105105pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6H2O30.02000219discardedA105
579
O105-2O10508-2518:00CaroGrowth527C4
Qiagen miniprep kit
Plasmid105105pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6H2O30.022214.3220A105II2312, II2313ok, ok, C->T in VF2 site
580
O106O10608-2518:00CaroGrowth528C1
Qiagen miniprep kit
Plasmid106106pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6H2O30.021025.5221A10676DC22ok
581
O106-2O10608-2518:00CaroGrowth528C3
Qiagen miniprep kit
Plasmid106106pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6H2O30.02870222discardedA106II2314, II2315mixture A64->T mutation, mixture A64->Tmutation
582
O107O10708-2518:00CaroGrowth529C1
Qiagen miniprep kit
Plasmid107107pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6H2O30.027018.9223
needs to be found or reperped
A107II2316, II2317ok, good, ok, C->T in VF2 site
583
O107-2O10708-2518:00CaroGrowth529C2
Qiagen miniprep kit
Plasmid107107pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6H2O30.025530.0224discardedA107
584
O108O10808-2518:00CaroGrowth530C3
Qiagen miniprep kit
Plasmid108108pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6H2O30.023713.1225A108II2318, II2319 1bp deletion before final H6, 1bp deletion in suffix, 1bp deletion before final H6, 1bp deletion in suffix
585
O108-2O10808-2518:00CaroGrowth530C2
Qiagen miniprep kit
Plasmid108108pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6H2O30.030123.7226A10876DC11, 76DC12mixture with T7RBS T6del, mixture with T7RBS T6del
586
O128O12808-2611:00CaroGrowth531C1
Qiagen miniprep kit
Plasmid128128pSBX1K3-T7RBS-H6-sfGFP-H6H2O30.024626.3A12876DB36, 76DB37perfect read, mutation in vf2 binding site??, insertion bbetween rbs and atg, perfect read, mutation in vf2 binding site??, insertion bbetween rbs and atg
587
O128-2O12808-2611:00CaroGrowth532C1
Qiagen miniprep kit
Plasmid128128pSBX1K3-T7RBS-H6-sfGFP-H6H2O30.013325.6A128#276DC15deletion in first stop codon, VF2C7T
588
O128-3O12808-2611:00CaroGrowth533C1
Qiagen miniprep kit
Plasmid128128pSBX1K3-T7RBS-H6-sfGFP-H6H2O30.019827.6A128#376DC16ok, VF2C7T
589
O122-2O12208-2611:00CaroGrowth534C1
Qiagen miniprep kit
Plasmid122122pSBX1K3-1GateNpuDnaE(N)H2O30.018530.0discardA122#3
590
O122-3O12208-2611:00CaroGrowth534C2
Qiagen miniprep kit
Plasmid122122pSBX1K3-1GateNpuDnaE(N)H2O30.028628.3A122#3II2320, II2321ok, perfect, ok, perfect
591
O123-3O12308-2611:00CaroGrowth535C1
Qiagen miniprep kit
Plasmid123123pSBX1K3-1GateNpuDnaE(C)H2O30.011530.0discardA123#2
592
O123-4O12308-2611:00CaroGrowth535C2
Qiagen miniprep kit
Plasmid123123pSBX1K3-1GateNpuDnaE(C)H2O30.018830.0discardA123#2
593
O123-5O12308-2611:00CaroGrowth535C3
Qiagen miniprep kit
Plasmid123123pSBX1K3-1GateNpuDnaE(C)H2O30.020827.6A123#2II2322, II2323ok, perfect, ok, perfect
594
R183R18308-2615:00CaroGel195-2Gel extraction
Restriction
183pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6 (X/P)H2O30.0826.5
595
R184R18408-2615:00CaroGel195-3Gel extraction
Restriction
184pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6 (X/P)H2O30.0725.4
596
R185R18508-2615:00CaroRestriction185
PCR purification kit
Restriction
185pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6 (S/P)H2O30.0926.7
597
R186R18608-2615:00CaroRestriction186
PCR purification kit
Restriction
186pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 (S/P)H2O30.01427.8
598
P173P17308-266:30 PMMaxWPCR739
PCR purification kit
PP173LambdaLys_BsaI_c-ext_BsaIH2O30.02115.9
599
P174P17408-2618:30MaxWPCR740
PCR purification kit
PP174ccdB_BsmBI-BsaI_BsaI-BsmBIH2O30.011218.4
600
O94-8O9408-2610:00 PMJakobPurificationO94-3DilutionPlasmid9494pSBX4C5-2GateNpuDnaE(N)H2O306.01278.8
601
O95-8O9508-2610:00 PMJakobPurificationO95-3DilutionPlasmid9595pSBX4C5-2GateNpuDnaE(C)H2O278.01250.8
602
R187R18708-2622:15SilvanGel
197-1-600bp,197-2-600bp
Gel extraction
Restriction
187pSB1A3-Smt3-2ZSortA-H6 (ccdB) (E/XmaI)EB30.0825.0
603
R188R18808-2622:15SilvanGel
197-4-500bp,197-5-500bp
Gel extraction
Restriction
188pSB1A3-Smt3-2ZSortA-H6 (ccdB) (XmaI/P)EB30.0725.6
604
P176P17608-2710:00 AMJakobGel208-2-1Gel extractionPP176BsmBI-ccdB-BsmBI_pSBX4C5_BsaI-mRFP-BsaIH2O30.01230.0
605
P177P17708-2710:00 AMJakobGel208-3-1Gel extractionPP177BsaI-mRFP-BsaI_pSBX4C5_BsmBI-ccdB-BsmBIH2O30.02230.0
606
P175P17508-2710:30LisaGel202-2, 202-3Gel extractionPP175pSB1C3-BBa_K741002_MT_SspDnaBNH2O30.01928.0
607
P187P18708-2713:40LisaOligos185,193AnnealingPP187CPEC_GFP_Insert_oligo250.0
608
O55-11O5508-27SilvanGrowth209C8
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)H2O30.04119.1A5576DB38single base insertion in Smt3/ccdb CPEC homology region
609
O55-12O5508-27SilvanGrowth211C6
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)H2O30.024328.1A5576DB39single base deletion in Smt3/ccdb CPEC homology region
610
O55-13O5508-27SilvanGrowth211C7
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)H2O30.029226.9A5576DB40, 76DB64ok, ok
611
O55-14O5508-27SilvanGrowth211C8
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)H2O30.023526.2A5576DB41, 76DB65ok, ok
612
O55-15O5508-27SilvanGrowth211C9
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)H2O30.027626.7A5576DB42, 76DB66ok, bad sequencing
613
O55-16O5508-27SilvanGrowth211C10
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)H2O30.021625.8A5576DB43, 76DB67ok, ok
614
O55-17O5508-27SilvanGrowth211C11
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)H2O30.035528.7A5576DB44single base deletion in backbone/Smt3 CPEC homology region
615
O55-18O5508-27SilvanGrowth212C7
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)H2O30.038127.6A55#276DB45, 76DB68ok, ok (one T not called)
616
O55-19O5508-27SilvanGrowth212C8
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)H2O30.024226.3A55#276DB46, 76DB69ok, ok
617
O55-20O5508-27SilvanGrowth212C9
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)H2O30.0227-11.4A55#276DB47, 76DB70ok, ok
618
O55-21O5508-27SilvanGrowth212C10
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)H2O30.023926.2A55#276DB48, 76DB71ok, mixture
619
O55-22O5508-27SilvanGrowth212C11
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)H2O30.023526.2A55#276DB49, 76DB72ok, ok
620
O55-23O5508-27SilvanGrowth212C12
Qiagen miniprep kit
Plasmid5555pSB1A3-Smt3-2ZSortA-H6 (ccdB)H2O30.025326.4A55#276DB50, 76DB34ok, ok
621
O125-4O12508-27SilvanGrowth544C1
Qiagen miniprep kit
Plasmid125125pSB1C3-Smt3-2ZSortA-H6 (ccdB)H2O30.014323.7227L125#276DB51, 76DB57negative, contains GFP, negative, contains GFP
622
O125-5O12508-27SilvanGrowth544C2
Qiagen miniprep kit
Plasmid125125pSB1C3-Smt3-2ZSortA-H6 (ccdB)H2O30.012022.5228L125#276DB52, 76DB58negative, contains GFP, negative, contains GFP
623
O125-6O12508-27SilvanGrowth544C3
Qiagen miniprep kit
Plasmid125125pSB1C3-Smt3-2ZSortA-H6 (ccdB)H2O30.010421.3229L125#276DB53, 76DB59negative, contains GFP, negative, contains GFP
624
O125-7O12508-27SilvanGrowth544C4
Qiagen miniprep kit
Plasmid125125pSB1C3-Smt3-2ZSortA-H6 (ccdB)H2O30.09720.8230L125#276DB54, 76DB60negative, contains GFP, negative, contains GFP
625
O125-8O12508-27SilvanGrowth544C5
Qiagen miniprep kit
Plasmid125125pSB1C3-Smt3-2ZSortA-H6 (ccdB)H2O30.07718.3231L125#276DB55, 76DB61negative, contains GFP, negative, contains GFP
626
O125-9O12508-27SilvanGrowth544C6
Qiagen miniprep kit
Plasmid125125pSB1C3-Smt3-2ZSortA-H6 (ccdB)H2O30.016624.6232L125#276DB56, 76DB62negative, contains GFP, negative, contains GFP
627
R189R18908-27AnnaGel205-2, 205-3Gel extraction
Restriction
189pSB1AK8-BBa_I712074 (S/P)H2O30.01115.4
628
R190R19008-27AnnaGel205-4, 205-5Gel extraction
Restriction
190pSB1A2-BBa_I13507 (X/P)H2O30.01017.9
629
O94-9O9408-2711:00 PMJakobGrowth525C1-1
Qiagen miniprep kit
Plasmid9494pSBX4C5-2GateNpuDnaE(N)H2O30.029130.0233yS94#4
630
O94-10O9408-2711:00 PMJakobGrowth525C2-1
Qiagen miniprep kit
Plasmid9494pSBX4C5-2GateNpuDnaE(N)H2O30.035026.6234yS94#4
631
O95-9O9508-2711:00 PMJakobGrowth526C1-1
Qiagen miniprep kit
Plasmid9595pSBX4C5-2GateNpuDnaE(C)H2O30.026330.0235yS95#4
632
O95-10O9508-2711:00 PMJakobGrowth526C2-1
Qiagen miniprep kit
Plasmid9595pSBX4C5-2GateNpuDnaE(C)H2O30.021624.4236yS95#4
633
R199R19908-2711:00 PMCaroGel210-2, 210-3Gel extraction
Restriction
199pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6 (X/P)H2O30.0824.6
634
R200R20008-2711:00 PMCaroGel 210-4,210-5Gel extraction
Restriction
200pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6 (X/P)H2O30.0824.0
635
R185-2R18508-2711:00 PMCaroRestriction185
PCR purification kit
Restriction
185pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6 (S/P)H2O30.0224.6
636
R186-2R18608-2711:00 PMCaroRestriction186
PCR purification kit
Restriction
186pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 (S/P)H2O30.0425.3
637
R185-3R18508-2711:00 PMCaroRestriction185
PCR purification kit
Restriction
185pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6 (S/P)H2O30.01227.3
638
R186-3R18608-2711:00 PMCaroRestriction186
PCR purification kit
Restriction
186pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 (S/P)H2O30.01127.2
639
R203R20308-280:12MaxWOligos94,95Annealing
Restriction
203AssemBIyIntN-linker+H6 ("GATG"/"GGTG")
T4L Buffer
100.017100.0
640
R204R20408-280:12MaxWOligos96,97Annealing
Restriction
204AssemBIyIntC-linker+H6 ("GATG"/"GGTG")
T4L Buffer
100.015100.0
641
P181P18108-282:00 AMJakobOligos86,87MixPP181SspDnaX-S11C
H2O DNase free ;-)
0.0
642
P183P18308-282:00 AMJakobOligos88,89MixPP183Ssp GyrB-S11C
H2O DNase free ;-)
0.0
643
P185P18508-282:00 AMJakobOligos90,91MixPP185Ter DnaE3-S11C
H2O DNase free ;-)
0.0
644
P178P17808-282:00 AMJakobExternGeneArtResuspendedPP178gp41-1N
H2O DNase free ;-)
15.05015.0
645
P179P17908-282:00 AMJakobExternGeneArtResuspendedPP179gp41-1C
H2O DNase free ;-)
10.05010.0
646
P180P18008-282:00 AMJakobExternGeneArtResuspendedPP180Ssp DnaX-S11N
H2O DNase free ;-)
33.05033.0
647
P182P18208-282:00 AMJakobExternGeneArtResuspendedPP182Ssp GyrB-S11N
H2O DNase free ;-)
25.05025.0
648
P184P18408-282:00 AMJakobExternGeneArtResuspendedPP184Ter DnaE3-S11N
H2O DNase free ;-)
21.05021.0
649
O131O13108-2815:30MaxWGrowth543C5
Qiagen miniprep kit
Plasmid131131pSBX4C5-2Zv2Npu:LaLys+ccdbH2O30.00238discardedG13176DB75, 76DB80gfp..............................., gfp...............................
650
O131-2O13108-2815:30MaxWGrowth543C7
Qiagen miniprep kit
Plasmid131131pSBX4C5-2Zv2Npu:LaLys+ccdbH2O30.00239discardedG13176DB76, 76DB81ccdb missing, ccdb missing
651
O132O13208-2816:30LisaGrowth547C1
Qiagen miniprep kit
Plasmid132132pSB1C3-Plac-GFP-IntN-MTSH2O30.019425.4240discardedA13276DB83, 76DB84is actually psbx ssp h6 gfp, is actually psbx ssp h6 gfp
652
O132-2O13208-2816:30LisaGrowth547C2
Qiagen miniprep kit
Plasmid132132pSB1C3-Plac-GFP-IntN-MTSH2O30.017630.0241discardedA132
653
O132-3O13208-2816:30LisaGrowth547C3
Qiagen miniprep kit
Plasmid132132pSB1C3-Plac-GFP-IntN-MTSH2O30.011330.0242discardedA132
654
O57-5O5708-2816:30LisaGrowth234C1-1
Qiagen miniprep kit
Plasmid5757pSBX1A3-BBa_I13507H2O30.025825.168to be used furtherL57#5
655
O129O12908-2817:50CaroGrowth552C1
Qiagen miniprep kit
Plasmid129129pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPCH2O30.0#DIV/0!S129#376DB89, 76DB90ok, ok
656
R196R19608-2820:00MaxWGel214-2, 214-3Gel extraction
Restriction
196pSBX4C5-2Zv2Npu (BsaI)H2O30.01523.2
657
R197R19708-2820:30MaxWRestriction197
PCR purification kit
Restriction
197LambdaLys_BsaI_c-ext_BsaI (BsaI)H2O20.01216.7
658
R198R19808-2820:30MaxWRestriction198
PCR purification kit
Restriction
198ccdB_BsmBI-BsaI_BsaI-BsmBI (BsmBI)H2O20.01215.2
659
O129-2O12928-0821:30CaroGrowth552C2
Qiagen miniprep kit
Plasmid129129pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPCH2O30.00243discardedS129#3
660
O129-3O12928-0821:30CaroGrowth552C3
Qiagen miniprep kit
Plasmid129129pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPCH2O30.00244discardedS129#3
661
O129-4O12928-0821:30CaroGrowth552C4
Qiagen miniprep kit
Plasmid129129pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPCH2O30.00discardedS129#3
662
O129-5O12928-0821:30CaroGrowth552C5
Qiagen miniprep kit
Plasmid129129pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPCH2O30.00discardedS129#3
663
R206R20628-0821:45LisaRestriction206
PCR purification kit
Restriction
206SspDnaBC_M+H6_N->A+SGG (X/P)H2O30.02120.0
664
R207R20728-0821:45LisaRestriction207
PCR purification kit
Restriction
207SspDnaBC_M-noH6_N->A+SGG (X/P)H2O30.02524.4
665
R205R20529-092:00LisaGel215-2, 215-3Gel extraction
Restriction
205pSB1C3-BBa_K741002 (X/P)H2O30.0178.0
666
O111-3O11108-2912:30MaxWGrowth501C1-1
Qiagen miniprep kit
Plasmid111111pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)H2O50.012638.0G111#276BD93, 76BD94ok, ok
667
O111-4O11108-2912:30MaxWGrowth501C2-1
Qiagen miniprep kit
Plasmid111111pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)H2O50.011041.0G111#276BD95, 76BD96ok, ok
668
O110-4O11008-2912:30MaxWGrowth476C3-1
Qiagen miniprep kit
Plasmid110110pSBX1K3-2Zv2Npu:(LambdaLys-H6)H2O50.09434.7G110#276BD97, 76BD98ok, ok
669
O137O13708-2918:00JakobGrowth564C6
Qiagen miniprep kit
Plasmid137137pSBX4C5-1Gate-NpuDnaE(N)-H6H2O30.048030.0discardedG137
670
O138O13808-296:00 PMJakobGrowth565C6
Qiagen miniprep kit
Plasmid138138pSBX4C5-H6-1GateNpuDnaE(C)H2O30.078030.0discardedG138
671
P176-2P17608-2712:00JakobGel212-2-1, 212-3-1Gel extractionPP176BsmBI-ccdB-BsmBI_pSBX4C5_BsaI-mRFP-BsaIH2O30.01830.0
672
P177-2P17708-2712:00JakobGel212-4-1, 212-5-1Gel extractionPP177BsaI-mRFP-BsaI_pSBX4C5_BsmBI-ccdB-BsmBIH2O30.02430.0
673
P173-2P17308-3016:30MaxWGel
223-2, 223-3, 223-4, 223-5
Gel extractionPP173LambdaLys_BsaI_c-ext_BsaIH2O30.023925.8
674
R208R20808-3020:40CaroGel224-2, 224-3Gel extraction
Restriction
208pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6 (X/P)H2O30.0814.0
675
R209R20908-3020:40CaroRestriction209
PCR purification kit
Restriction
209pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 (S/P)H2O30.01425.2
676
O113-3O11308-3022:15MaxWGrowth562C1
Qiagen miniprep kit
Plasmid113113pSBX1K3-2Zv2Npu:Xyla_circ_noHisH2O50.015040.0245G113#376DC23, 76DC24ok, ok
677
O113-4O11308-3022:15MaxWGrowth562C4
Qiagen miniprep kit
Plasmid113113pSBX1K3-2Zv2Npu:Xyla_circ_noHisH2O50.015036.7246G113#376DB87, 76DB88ok, ok
678
R214R21408-3022:15AnnaGel226-2, 226-3Gel extraction
Restriction
214pSB1A3-Smt3-2ZSortA-H6 (ccdB) (E/P)EB30.0830.0
679
R215R21508-3022:15AnnaGel226-4Gel extraction
Restriction
215pSBX4A5-BBa_I13507 (E/P)EB30.054.5
680
R210R21008-3022:45MaxWGel225-2, 225-3Gel extraction
Restriction
210pSBX4C5-2Zv2Npu (BsaI)H2O30.05323.1
681
R211R21108-3022:45MaxWGel225-4, 225-5Gel extraction
Restriction
211pSBX1K3-2Zv2Npu (BsaI)H2O30.02721.8
682
R212R21208-3022:45MaxWRestriction212
PCR purification kit
Restriction
212LambdaLys_BsaI_c-ext_BsaI (BsaI)H2O20.04012.3
683
R213R21308-3022:45MaxWRestriction213
PCR purification kit
Restriction
213ccdB_BsmBI-BsaI_BsaI-BsmBI (BsmBI)H2O20.0408.8
684
O137-2O13708-3111:10JakobGrowth564C1
Qiagen miniprep kit
Plasmid137137pSBX4C5-1Gate-NpuDnaE(N)-H6H2O30.030.0discardedG137
685
O137-3O13708-3111:10JakobGrowth564C2
Qiagen miniprep kit
Plasmid137137pSBX4C5-1Gate-NpuDnaE(N)-H6H2O30.030.0discardedG137
686
O138-2O13808-3111:10JakobGrowth565C1
Qiagen miniprep kit
Plasmid138138pSBX4C5-H6-1GateNpuDnaE(C)H2O30.030.0discardedG138
687
O138-3O13808-3111:10JakobGrowth565C2
Qiagen miniprep kit
Plasmid138138pSBX4C5-H6-1GateNpuDnaE(C)H2O30.030.0discardedG138
688
O108-3O108todoCaroGrowth530C1-1
Qiagen miniprep kit
Plasmid108108pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H60.0A108
689
R217R21709-0112:00LisaGel238-2, 238-3Gel extraction
Restriction
217pSB1C3-BBa_K741002 (X/P)H2O30.03015.9
690
R216R21609-0114:00CaroRestriction216
PCR purification kit
Restriction
216pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 (S/P)H2O30.01425.3
691
O140O14009-0115:00LisaGrowth567C2
Qiagen miniprep kit
Plasmid139139pSB1C3-T7RBS_+His_SspDnaBC_ExtH2O50.026242.7273
whoop how did that happen?
S14076DB99, 76DC00it has a his tag (the two plates were mixed up) -> juhu! intein protease ready,
692
O140-2O14009-0115:00LisaGrowth567C3
Qiagen miniprep kit
Plasmid139139pSB1C3-T7RBS_+His_SspDnaBC_ExtH2O50.024050.0274discardedS140
693
R218R21809-0113:00LisaGel237-2, 237-3Gel extraction
Restriction
218SspDnaBC_M+H6_N->A+SGG (X/P)H2O30.02225.9
694
R219R21909-0113:00LisaGel237-4, 237-5Gel extraction
Restriction
219SspDnaBC_M-noH6_N->A+SGG (X/P)H2O30.01921.8
695
O131-3O13109-0115:30MaxWGrowth583C1
Qiagen miniprep kit
Plasmid131131pSBX4C5-2Zv2Npu:LaLys+ccdbH2O50.026950.0249discardedL131#2
696
O131-4O13109-0115:30MaxWGrowth583C2
Qiagen miniprep kit
Plasmid131131pSBX4C5-2Zv2Npu:LaLys+ccdbH2O50.029846.8250L131#276DC01, 76DC02ok, ok
697
O131-5O13109-0115:30MaxWGrowth583C4
Qiagen miniprep kit
Plasmid131131pSBX4C5-2Zv2Npu:LaLys+ccdbH2O50.033650.0251discardedL131#2
698
O131-6O13109-0115:30MaxWGrowth583C6
Qiagen miniprep kit
Plasmid131131pSBX4C5-2Zv2Npu:LaLys+ccdbH2O50.038650.0252discardedL131#2
699
O141O14109-0115:30MaxWGrowth584C1
Qiagen miniprep kit
Plasmid141141pSBX1K3-2Zv2Npu:LaLys+ccdbH2O50.010850.0253discardedL141
700
O141-2O14109-0115:30MaxWGrowth584C3
Qiagen miniprep kit
Plasmid141141pSBX1K3-2Zv2Npu:LaLys+ccdbH2O50.035346.8254L14176DC03, 76DC04ok, ok
701
O141-3O14109-0115:30MaxWGrowth584C5
Qiagen miniprep kit
Plasmid141141pSBX1K3-2Zv2Npu:LaLys+ccdbH2O50.030250.0255discardedL141
702
O131-7O13109-0115:30MaxWGrowth585C5
Qiagen miniprep kit
Plasmid131131pSBX4C5-2Zv2Npu:LaLys+ccdbH2O50.035146.8257G131#276DC05, 76DC06ok, ok
703
O131-8O13109-0115:30MaxWGrowth585C6
Qiagen miniprep kit
Plasmid131131pSBX4C5-2Zv2Npu:LaLys+ccdbH2O50.036746.8258G131#276DC07, 76DC08ok, ok
704
O131-9O13109-0115:30MaxWGrowth585C7
Qiagen miniprep kit
Plasmid131131pSBX4C5-2Zv2Npu:LaLys+ccdbH2O50.048050.0259discardedG131#2
705
O131-10O13109-0115:30MaxWGrowth585C9
Qiagen miniprep kit
Plasmid131131pSBX4C5-2Zv2Npu:LaLys+ccdbH2O50.041050.0260discardedG131#2
706
O141-4O14109-0115:30MaxWGrowth586C5
Qiagen miniprep kit
Plasmid141141pSBX1K3-2Zv2Npu:LaLys+ccdbH2O50.016050.0261discardedG141
707
O141-5O14109-0115:30MaxWGrowth586C8
Qiagen miniprep kit
Plasmid141141pSBX1K3-2Zv2Npu:LaLys+ccdbH2O50.014150.0262discardedG141
708
O141-6O14109-0115:30MaxWGrowth586C9
Qiagen miniprep kit
Plasmid141141pSBX1K3-2Zv2Npu:LaLys+ccdbH2O50.033046.8263discardedG14176DC09, 76DC10mutation in ccdb (R->H), mutation in ccdb (R->H)
709
R208-2R20808-2715:00CaroGel210-2,210-3Gel extraction
Restriction
208pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6 (X/P)H2O100.0
710
O108-4O10809-0118:30JakobGrowth530C1-1
Qiagen miniprep kit
Plasmid108108pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6H2O30.050027.2A10876DC13, 76DC14ok, ok
711
O137-4O13709-0118:30JakobGrowth564C3
Qiagen miniprep kit
Plasmid137137pSBX4C5-1Gate-NpuDnaE(N)-H6H2O30.070027.3264G13776DC17, 76DC18ok, ok
712
O138-4O13809-0118:30JakobGrowth565C3
Qiagen miniprep kit
Plasmid138138pSBX4C5-H6-1GateNpuDnaE(C)H2O30.052026.3265G13876DC20, 76DC21ok, ok
713
O103-5O10309-0123:45MaxWPurificationO103-4DilutionPlasmid103103pSBX1K3-2Zv2NpuH2O87.0185.0
714
P197P19709-0112:00JakobGel
241-2-1, 241-3-1, 241-4-1
Gel extractionPP197ccdB-pSBX4C5-mRFP(N)_H2O30.01630.0
715
P198P19809-0112:00JakobGel242-2-1, 242-3-1Gel extractionPP198mRFP-pSBX4C5-ccdB-(C)_H2O30.01830.0
716
0-40Caro0?H2O30.030.0
717
0-50Caro0?H2O30.030.0
718
O133O13309-0216:00AnnaGrowth595C1
Qiagen miniprep kit
Plasmid133133pSB1AK8-BBa_I712074-BBa_I13507H2O30.040266discardedS133
719
O133-2O13309-0216:00AnnaGrowth595C1
Qiagen miniprep kit
Plasmid133133pSB1AK8-BBa_I712074-BBa_I13507H2O30.04730.0266S133
720
O133-3O13309-0216:00AnnaGrowth595C2
Qiagen miniprep kit
Plasmid133133pSB1AK8-BBa_I712074-BBa_I13507H2O30.06830.0267S133
721
O133-4O13309-0216:00AnnaGrowth595C3
Qiagen miniprep kit
Plasmid133133pSB1AK8-BBa_I712074-BBa_I13507H2O30.07230.0268S133
722
O137-5O13709-0217:00CaroPurificationO137-4DilutionPlasmid137137pSBX4C5-1Gate-NpuDnaE(N)-H635.02035.0
723
O138-5O13809-0217:00CaroPurificationO138-4DilutionPlasmid138138pSBX4C5-H6-1GateNpuDnaE(C)26.02026.0
724
P97-2P9709-0217:00CaroPurificationP97DilutionPP97mRFPn_1-1547.00207.00
725
P99-2P9909-0217:00CaroPurificationP99DilutionPP99mRFPc_155-2258.00208.00
726
P98-2P9809-0217:00CaroPurificationP98DilutionPP98mRFP_1-1686.00206.00
727
P100-2P10009-0217:00CaroPurificationP100DilutionPP100mRFPc_169-2258.00208.00
728
P101-2P10109-0217:00CaroPurificationP101DilutionPP101RenLucN_1-229_H6_GATG_BsaI_KGG_TGCT_BsaI8.00208.00
729
P102-2P10209-0217:00CaroPurificationP102DilutionPP102RenLucC_230-311_NWC_CAAC_BsaI_H6_TAAT_BsaI8.50208.50
730
P103-2P10309-0217:00CaroPurificationP103DilutionPP103FireLucN_1-437_H6_GATG_BsaI_KGG_TGCT_BsaI4.50204.50
731
P104-2P10409-0217:00CaroPurificationP104DilutionPP104FireLucC_438-550_NWC_CAAC_BsaI_H6_TAAT_BsaI8.00208.00
732
P105-2P10509-0217:00CaroPurificationP105DilutionPP105mCherryN_1-168_H6_GATG_BsaI_KGG_TGCT_BsaI2.50202.50
733
P106-2P10609-0217:00CaroPurificationP106DilutionPP106mCherryC_169-236_NWC_CAAC_BsaI_H6_TAAT_BsaI6.00206.00
734
P107-2P10709-0217:00CaroPurificationP107DilutionPP107GFPn_1-64_H6_BsaI5.00205.00
735
P109-2P10909-0217:00CaroPurificationP109DilutionPP109GFPc_65-238_NWC_CAAC_BsaI_H6_TAAT_BsaI6.00206.00
736
P108-2P10809-0217:00CaroPurificationP108DilutionPP108GFPn_1-157_H6_BsaI8.00208.00
737
P110-2P11009-0217:00CaroPurificationP110DilutionPP110GFPc_158-238_NWC_CAAC_BsaI_H6_TAAT_BsaI5.00205.00
738
R221R22109-0219:30LisaGel246-2, 246-3Gel extraction
Restriction
221pSB1C3-BBa_K808000 (E/S)H2O30.081.4
739
R220R22009-0219:30LisaRestriction220
PCR purification kit
Restriction
220pSB1C3-T7RBS_+His_SspDnaBC_Ext (E/X)H2O30.0625.8
740
P199P19909-0221:30MaxWGel247-2, 247-3Gel extractionPP199p2Zv2Npu_Smt3_flag_Smt3H2O30.03430.0
741
P200P20009-0221:30MaxWGel247-4, 247-5Gel extractionPP200Smt3_p2Zv2Npu_flag_p2Zv2NpuH2O30.05730.0
742
O130O13009-030:30CaroGrowth601C1
Qiagen miniprep kit
Plasmid130130pSBX1K3-sfGFPN-NpuDnaEN-C->G_NpuDnaEC-sfGFPC-SN->AGH2O50.06050.0275S130#5BI2119, BI2120,
743
O130-2O13009-030:30CaroGrowth602C1
Qiagen miniprep kit
Plasmid130130pSBX1K3-sfGFPN-NpuDnaEN-C->G_NpuDnaEC-sfGFPC-SN->AGH2O50.07450.0276discardedS130#5
744
P160P16009-0322:00LisaGel253-2, 253-3, 253-4Gel extractionPP160pSB1C3-BBa_K741002-Ter_F_KpnI-Ext_IntN_BamHIH2O30.03330.0
745
P217P21709-0321:35SilvanGel
252-2-2.7kb,252-3-2.7kb
Gel extractionPP217BsaI=GGGT-G4-DNMT1-731-1602-Flag-CTTC=BsaIEB30.0282.7
746
P218P21809-0321:35SilvanGel
252-4-1.1kb,252-5-1.1kb
Gel extractionPP218CGCA=BsaI-PlacI-mRFP-BsaI=CTTCEB30.010220.2
747
R222R22209-0322:15SilvanRestriction222
PCR purification kit
Restriction
222BsaI=GGGT-G4-DNMT1-731-1602-Flag-CTTC=BsaI (BsaI)EB30.01130.0
748
R223R22309-0322:15SilvanRestriction223
PCR purification kit
Restriction
223CGCA=BsaI-PlacI-mRFP-BsaI=CTTC (BsaI)EB30.02630.0
749
R224R22409-0322:15SilvanRestriction224
PCR purification kit
Restriction
224mDNMT1(731-1602)_BsaI=GGGT-GGG_CGCA=BsaI (BsaI)EB30.01530.0
750
R225R22509-0323:45SilvanGel261-2-2.5kbGel extraction
Restriction
225pSBX1K3-BBa_I13507 (E/P)EB15.01515.0
751
R226R22609-0323:45SilvanGel
261-3-1.1kb,261-4-1.1kb
Gel extraction
Restriction
226pSB1A3-Smt3-2ZSortA-H6 (ccdB) (E/P)EB15.01015.0
752
O59-12O5909-0416:40SilvanGrowth236C1-1
Qiagen maxiprep kit
Plasmid5959pSBX1K3-BBa_I13507EB400.0823384.6L59#5
753
O160O16009-0416:40MaxWGrowth610C1
Qiagen miniprep kit
Plasmid160160pSBX1K3-T7RBS-FLAG-Smt3-2Zv2NpuH2O50.029950.0283discardedA160BI2115, BI2116mutations in terminator(g->a), in mrfp (still red anyway), in smt3 (Q->R), mutations in terminator(g->a), in mrfp (still red anyway), in smt3 (Q->R)
754
O160-2O16009-0416:40MaxWGrowth610C2
Qiagen miniprep kit
Plasmid160160pSBX1K3-T7RBS-FLAG-Smt3-2Zv2NpuH2O50.034750.0284A160BI2117, BI2118bad sequencing, auch eine in mrfp
755
O160-3O16009-0416:40MaxWGrowth610C3
Qiagen miniprep kit
Plasmid160160pSBX1K3-T7RBS-FLAG-Smt3-2Zv2NpuH2O50.037250.0285discardedA160BI2147, BI2148insertion in smt3, silent in mfrp
756
O160-4O16009-0416:40MaxWGrowth610C4
Qiagen miniprep kit
Plasmid160160pSBX1K3-T7RBS-FLAG-Smt3-2Zv2NpuH2O50.038748.7286A160BI2149, BI2150, AF9230ok, ok (silent in mrfp),
757
O159O15909-0416:40LisaGrowth609C1
Qiagen miniprep kit
Plasmid159159pSB1C3-K808000_T7RBS_+His_SspDnaBC_ExtH2O50.013650.0289discardedS159
758
O159-2O15909-0416:40LisaGrowth609C3
Qiagen miniprep kit
Plasmid159159pSB1C3-K808000_T7RBS_+His_SspDnaBC_ExtH2O50.032550.0290discardedS159
759
O159-3O15909-0416:40LisaGrowth609C5
Qiagen miniprep kit
Plasmid159159pSB1C3-K808000_T7RBS_+His_SspDnaBC_ExtH2O50.018845.2291S15976DB85, 76DB86ok, ok, juhu, one part of intein protease ready, you should ring bell
760
R228R22809-0418:45SilvanGel263-2-xGel extraction
Restriction
228pSB1A3-Smt3-2ZSortA-H6 (ccdB) (X/P)EB15.0173.7
761
O145O14509-0418:20CaroGrowth612C1
Qiagen miniprep kit
Plasmid145145pSBX4C5-H6-mRFPn1-154-NpuN-H6H2O600150292G145BI2121, BI2122,
762
O145-2O14509-0418:20CaroGrowth612C2
Qiagen miniprep kit
Plasmid145145pSBX4C5-H6-mRFPn1-154-NpuN-H6H2O600148293G145
763
O146O14609-0418:20CaroGrowth613C2
Qiagen miniprep kit
Plasmid146146pSBX4C5-H6-NpuC-mRFP155-225-H6H2O600208294G146BI2123, BI2124,
764
O146-2O14609-0418:20CaroGrowth613C3
Qiagen miniprep kit
Plasmid146146pSBX4C5-H6-NpuC-mRFP155-225-H6H2O600115295G146
765
O147O14709-0418:20CaroGrowth614C1
Qiagen miniprep kit
Plasmid147147pSBX4C5-H6-mRFP1-168-NpuN-H6H2O600104296G147BI2125, BI2126,
766
O147-2O14709-0418:20CaroGrowth614C2
Qiagen miniprep kit
Plasmid147147pSBX4C5-H6-mRFP1-168-NpuN-H6H2O600117297G147
767
O148O14809-0418:20CaroGrowth615C2
Qiagen miniprep kit
Plasmid148148pSBX4C5-H6-NpuC-mRFP169-225-H6H2O60029298G148BI2127, BI2128,
768
O148-2O14809-0418:20CaroGrowth615C3
Qiagen miniprep kit
Plasmid148148pSBX4C5-H6-NpuC-mRFP169-225-H6H2O60056299G148
769
O149O14909-0418:20CaroGrowth616C2
Qiagen miniprep kit
Plasmid149149pSBX4C5-H6-RenLucN-NpuN-H6H2O60019300G149BI2129, BI2130,
770
O149-2O14909-0418:20CaroGrowth616C3
Qiagen miniprep kit
Plasmid149149pSBX4C5-H6-RenLucN-NpuN-H6H2O60069301G149
771
O150O15009-0418:20CaroGrowth617C2
Qiagen miniprep kit
Plasmid150150pSBX4C5-H6-NpuC-RenLucC-H6H2O60034302G150BI2131, BI2132,
772
O150-2O15009-0418:20CaroGrowth617C4
Qiagen miniprep kit
Plasmid150150pSBX4C5-H6-NpuC-RenLucC-H6H2O60064303G150
773
O151O15109-0418:20CaroGrowth618C2
Qiagen miniprep kit
Plasmid151151pSBX4C5-H6-FireLucN-NpuN-H6H2O600111304G151BI2133, BI2134,
774
O151-2O15109-0418:20CaroGrowth618C3
Qiagen miniprep kit
Plasmid151151pSBX4C5-H6-FireLucN-NpuN-H6H2O60070305G151
775
O152O15209-0418:20CaroGrowth619C2
Qiagen miniprep kit
Plasmid152152pSBX4C5-H6-NpuC-FireLucC-H6H2O6001306G152BI2135, BI2136,
776
O152-2O15209-0418:20CaroGrowth619C3
Qiagen miniprep kit
Plasmid152152pSBX4C5-H6-NpuC-FireLucC-H6H2O6001307G152
777
O153O15309-0418:20CaroGrowth620C2
Qiagen miniprep kit
Plasmid153153pSBX4C5-H6-mCherryN-NpuN-H6H2O6000308G153BI2137, BI2138,
778
O153-2O15309-0418:20CaroGrowth620C3
Qiagen miniprep kit
Plasmid153153pSBX4C5-H6-mCherryN-NpuN-H6H2O60023309G153
779
O154O15409-0418:20CaroGrowth621C2
Qiagen miniprep kit
Plasmid154154pSBX4C5-H6-NpuC-mCherryC-H6H2O6000310G154BI2139, BI2140,
780
O154-2O15409-0418:20CaroGrowth621C3
Qiagen miniprep kit
Plasmid154154pSBX4C5-H6-NpuC-mCherryC-H6H2O6000311G154
781
O155O15509-0418:20CaroGrowth622C1
Qiagen miniprep kit
Plasmid155155pSBX4C5-H6-GFPn1-64-NpuN-H6H2O6000312G155BI2141, BI2142,
782
O155-2O15509-0418:20CaroGrowth622C2
Qiagen miniprep kit
Plasmid155155pSBX4C5-H6-GFPn1-64-NpuN-H6H2O6000313G155
783
O157O15709-0418:20CaroGrowth624C2
Qiagen miniprep kit
Plasmid157157pSBX4C5-H6-GFPn1-157-NpuN-H6H2O6000314G157BI2143, BI2144,
784
O157-2O15709-0418:20CaroGrowth624C2
Qiagen miniprep kit
Plasmid157157pSBX4C5-H6-GFPn1-157-NpuN-H6H2O6000314G157
785
O158O15809-0418:20CaroGrowth625C1
Qiagen miniprep kit
Plasmid158158pSBX4C5-H6-GFPc158-238-NpuC-H6H2O6000316G158
786
O158-2O15809-0418:20CaroGrowth625C2
Qiagen miniprep kit
Plasmid158158pSBX4C5-H6-GFPc158-238-NpuC-H6H2O6000317G158
787
R227R22709-0419:00SilvanRestriction227
PCR purification kit
Restriction
227pSBX1K3-T7RBS_BBv2 (S/P)EB15.02910.7
788
P180-2P18009-0520:00MaxWPurificationP180DilutionPP180Ssp DnaX-S11NH2O50.0146.0
789
P182-2P18209-0520:00MaxWPurificationP182DilutionPP182Ssp GyrB-S11NH2O50.0146.0
790
P184-2P18409-0520:00MaxWPurificationP184DilutionPP184Ter DnaE3-S11NH2O50.0146.0
791
P197-2P19709-061:30MaxWGel265-2, 265-3Gel extractionPP197ccdB-pSBX4C5-mRFP(N)_H2O30.06530.0
792
P197-3P19709-061:30MaxWGel265-4, 265-4Gel extractionPP197ccdB-pSBX4C5-mRFP(N)_H2O30.06430.0
793
P197-4P19709-061:30MaxWGel265-6, 265-7Gel extractionPP197ccdB-pSBX4C5-mRFP(N)_H2O30.06230.0
794
P198-2P19809-061:30MaxWGel265-8, 265-9Gel extractionPP198mRFP-pSBX4C5-ccdB-(C)_H2O30.07430.0
795
P198-3P19809-061:30MaxWGel265-10, 265-11Gel extractionPP198mRFP-pSBX4C5-ccdB-(C)_H2O30.05030.0
796
P198-4P19809-061:30MaxWGel265-12, 265-13Gel extractionPP198mRFP-pSBX4C5-ccdB-(C)_H2O30.05130.0
797
P223P22309-061:30MaxWGel267-2, 267-3Gel extractionPP223Ssp DnaX-S11N_BsaI_mRFP_BsmBI_ccdBH2O20.018820.0
798
P224P22409-061:30MaxWGel267-4, 267-5Gel extractionPP224Ssp DnaX-S11N_C->G_BsaI_mRFP_BsmBI_ccdBH2O20.016920.0
799
P225P22509-061:30MaxWGel267-6, 267-7Gel extractionPP225Ssp GyrB-S11N_BsaI_mRFP_BsmBI_ccdBH2O20.016020.0
800
P226P22609-061:30MaxWGel267-8, 267-9Gel extractionPP226Ssp GyrB-S11N_C->G_BsaI_mRFP_BsmBI_ccdBH2O20.016620.0
801
P227P22709-061:30MaxWGel267-10, 267-11Gel extractionPP227Ter DnaE3-S11N_BsaI_mRFP_BsmBI_ccdBH2O20.015720.0
802
P228P22809-061:30MaxWGel267-12, 267-13Gel extractionPP228Ter DnaE3-S11N_C->G_BsaI_mRFP_BsmBI_ccdBH2O20.010620.0
803
P231P23109-063:50SilvanGel268-8, 268-9Gel extractionPP231mRFPexp_Smt3-TEVc-BsaI_mockSortAc-BBsuffixEB30.014630.0
804
P233P23309-063:50SilvanGel268-10,268-11Gel extractionPP233mRFPexp_H6-TEVc_mockSortAc-BBsuffixEB30.010730.0
805
P235P23509-063:50SilvanGel268-12,268-13Gel extractionPP235mRFPexp_TEVc-BsaI_SortAc-H6-BBsuffixEB30.013130.0
806
P236P23609-063:50SilvanGel268-15,268-16Gel extractionPP236eGFP_BsaI-G5RM_BsaIEB30.015330.0
807
P237P23709-063:50SilvanGel268-17,268-18Gel extractionPP237ccdB_BsaI-BsmBI_BsaI-BsmBIEB30.015930.0
808
P238P23809-063:50SilvanGel268-19,268-20Gel extractionPP238H6Smt3_BBprefix_TEVc-BsaIEB30.03230.0
809
P239P23909-063:50SilvanGel268-21,268-22Gel extractionPP239Npu DnaEC_BsmBI_ccdB_SN->AG-BsaI-mRFPEB30.05230.0
810
P240P24009-063:50SilvanGel268-23,268-24Gel extractionPP240NpuDnaEN_C->G-BsaI-mRFP_BsmBI_ccdBEB30.07930.0
811
P221P22109-0612:15JanGel264-2-1Gel extractionPP221143pSBX4C5-2GateNpuDnaEN C-GH2O10.02910.0
812
P222P22209-0612:15JanGel264-3-1Gel extractionPP222144pSBX4C5-2GateNpuDnaEC SN->AGH2O10.01810.0
813
P241P24109-0615:30MaxWOligos215,216MixPP241Ssp DnaX-S11C_BsmBI_ccdB_BsaI_mRFPH2O200.019200.0not real
814
P242P24209-0615:30MaxWOligos215,217MixPP242Ssp DnaX-S11C_BsmBI_ccdB_HN->AG_BsaI_mRFPH2O200.019200.0not real
815
P243P24309-0615:30MaxWOligos219,220MixPP243Ssp GyrB-S11C_BsmBI_ccdB_BsaI_mRFPH2O200.019200.0not real
816
P244P24409-0615:30MaxWOligos219,221MixPP244Ssp GyrB-S11C_BsmBI_ccdB_SN->AG_BsaI_mRFPH2O200.019200.0not real
817
P245P24509-0615:30MaxWOligos223,224MixPP245Ter DnaE3-S11C_BsmBI_ccdB_BsaI_mRFPH2O200.019200.0not real
818
P246P24609-0615:30MaxWOligos223,225MixPP246Ter DnaE3-S11C_BsmBI_ccdB_SN->AG_BsaI_mRFPH2O200.019200.0not real
819
O12-5O1209-0616:35NilsHPurificationO12DilutionPlasmid1212pUC19H2O50.0050.0
820
R229R22909-0620:00AnnaGel269-2Gel extraction
Restriction
229pSBX1K3-T7RBS_BBv2 (S/P)H2O30.03527.1
821
R230R23009-0620:00AnnaGel269-3Gel extraction
Restriction
230pSB1A3-Smt3-2ZSortA-H6 (ccdB) (X/P)H2O30.01416.9
822
R231R23109-0619:50LisaOligos178,179Annealing
Restriction
231BamHI-MTS_bs_minD-KpnI-**_F (BamHI/K)
T4Ligase Buffer
100.02996.1
823
O145-3O14509-0711:00CaroGrowth612C1-1
Qiagen miniprep kit
Plasmid145145pSBX4C5-H6-mRFPn1-154-NpuN-H6H2O30.026524.3G145BI2151, BI2163ok,
824
O146-3O14609-0711:00CaroGrowth613C2-1
Qiagen miniprep kit
Plasmid146146pSBX4C5-H6-NpuC-mRFP155-225-H6H2O30.033020.9G146BI2152, BI2164ok,
825
O147-3O14709-0711:00CaroGrowth614C1-1
Qiagen miniprep kit
Plasmid147147pSBX4C5-H6-mRFP1-168-NpuN-H6H2O30.036927.3G147BI2153, BI2165kok,
826
O148-3O14809-0711:00CaroGrowth615C2-1
Qiagen miniprep kit
Plasmid148148pSBX4C5-H6-NpuC-mRFP169-225-H6H2O30.025018.0G148BI2154ok
827
O149-3O14909-0711:00CaroGrowth616C2-1
Qiagen miniprep kit
Plasmid149149pSBX4C5-H6-RenLucN-NpuN-H6H2O30.017124.2G149BI2155, BI2166ok, one read bad, silent mutation in ligation renlucN to NpuN,
828
O150-3O15009-0711:00CaroGrowth617C2-1
Qiagen miniprep kit
Plasmid150150pSBX4C5-H6-NpuC-RenLucC-H6H2O30.018814.0G150BI2156ok, TAA insertion after His Tag
829
O151-3O15109-0711:00CaroGrowth618C2-1
Qiagen miniprep kit
Plasmid151151pSBX4C5-H6-FireLucN-NpuN-H6H2O30.016223.8G151BI2157, BI2167ok,
830
O152-3O15209-0711:00CaroGrowth619C2-1
Qiagen miniprep kit
Plasmid152152pSBX4C5-H6-NpuC-FireLucC-H6H2O30.032620.8G152BI2158ok
831
O153-3O15309-0711:00CaroGrowth620C2-1
Qiagen miniprep kit
Plasmid153153pSBX4C5-H6-mCherryN-NpuN-H6H2O30.016921.1G153BI2159, BI2168ok,
832
O154-3O15409-0711:00CaroGrowth621C2-1
Qiagen miniprep kit
Plasmid154154pSBX4C5-H6-NpuC-mCherryC-H6H2O30.053522.7G15476DB91, 76DB92ok, ok
833
O155-3O15509-0711:00CaroGrowth622C1-1
Qiagen miniprep kit
Plasmid155155pSBX4C5-H6-GFPn1-64-NpuN-H6H2O30.019230.0G155BI2160, BI2169ok,
834
O157-3O15709-0711:00CaroGrowth624C2-2
Qiagen miniprep kit
Plasmid157157pSBX4C5-H6-GFPn1-157-NpuN-H6H2O30.064628.5G157BI2161, BI2170ok,
835
O158-3O15809-0712:00CaroGrowth625C1-1
Qiagen miniprep kit
Plasmid158158pSBX4C5-H6-GFPc158-238-NpuC-H6H2O30.064025.3G158BI2162ok, one shift after "stem loop"
836
P230P23009-0720:00SilvanGel
275-2,275-3,275-4,275-5
Gel extractionPP230pSB1C3_mockSortAc_H6-Smt3EB15.011715.0
837
P232P23209-0720:00SilvanGel
275-6,275-7-275-8,275-9
Gel extractionPP232pSB1C3_mockSortAc_H6-TEVcEB15.03015.0
838
P234P23409-0720:00SilvanGel
275-10,275-11,275-12
Gel extractionPP234pSB1C3_SortAc-H6_TEVc-BsaIEB15.05815.0
839
O162O16209-0811:00AnnaGrowth666C2
Qiagen miniprep kit
Plasmid162162pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB)H2O50.02050.0S162#4
840
O162-2O16209-0811:00AnnaGrowth665C3
Qiagen miniprep kit
Plasmid162162pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB)H2O50.03550.0S162#3BI2196, BI2197,
841
O163O16309-0814:30MaxWGrowth646C1
Qiagen miniprep kit
Plasmid163163pSBX4C5-2GateSspDnaX-S11CH2O50.018850.0323used for 1GateA163BI2145, BI2171ok, ok
842
O163-2O16309-0814:30MaxWGrowth646C2
Qiagen miniprep kit
Plasmid163163pSBX4C5-2GateSspDnaX-S11C
H2O+X
50+X280324discardedA163
843
O164O16409-0814:30MaxWGrowth647C1
Qiagen miniprep kit
Plasmid164164pSBX4C5-2GateSspDnaX-S11C HN->AGH2O50.027050.0326used for 1GateA164BI2172, BI2173ok, ok
844
O164-2O16409-0814:30MaxWGrowth647C2
Qiagen miniprep kit
Plasmid164164pSBX4C5-2GateSspDnaX-S11C HN->AGH2O50.026650.0327discardedA164
845
O165O16509-0814:30MaxWGrowth648C1
Qiagen miniprep kit
Plasmid165165pSBX4C5-2GateSspDnaX-S11NH2O50.020050.0329A165BI2174, BI2175, BI1917part of intein+mrfp not sequenced, part of intein+mrfp not sequenced, ok
846
O165-2O16509-0814:30MaxWGrowth648C2
Qiagen miniprep kit
Plasmid165165pSBX4C5-2GateSspDnaX-S11NH2O50.017550.0330discardedA165
847
O166O16609-0814:30MaxWGrowth649C1
Qiagen miniprep kit
Plasmid166166pSBX4C5-2GateSspDnaX-S11N C->G
H2O+X
50+X53#VALUE!332A166BI2176, BI2177, BI1918part of intein+mrfp not sequenced, part of intein+mrfp not sequenced, ok
848
O166-2O16609-0814:30MaxWGrowth649C2
Qiagen miniprep kit
Plasmid166166pSBX4C5-2GateSspDnaX-S11N C->G
H2O+X
50+X60#VALUE!333discardedA166
849
O167O16709-0814:30MaxWGrowth650C1
Qiagen miniprep kit
Plasmid167167pSBX4C5-2GateSspGyrB-S11CH2O50.023450.0335A167BI2178, BI2179ok, ok
850
O167-2O16709-0814:30MaxWGrowth650C2
Qiagen miniprep kit
Plasmid167167pSBX4C5-2GateSspGyrB-S11CH2O50.025750.0336discardedA167
851
O168O16809-0814:30MaxWGrowth651C1
Qiagen miniprep kit
Plasmid168168pSBX4C5-2GateSspGyrB-S11C SN->AGH2O50.023550.0338A168BI2180, BI2181ok, ok
852
O168-2O16809-0814:30MaxWGrowth651C2
Qiagen miniprep kit
Plasmid168168pSBX4C5-2GateSspGyrB-S11C SN->AGH2O50.026650.0339discardedA168
853
O169O16909-0814:30MaxWGrowth652C1
Qiagen miniprep kit
Plasmid169169pSBX4C5-2GateSspGyrB-S11NH2O50.020550.0341A169BI2182, BI2183, BI1919part of intein+mrfp not sequenced, part of intein+mrfp not sequenced, mutation in ccdb c->a (?), ok, only intein sequenzed
854
O169-2O16909-0814:30MaxWGrowth652C2
Qiagen miniprep kit
Plasmid169169pSBX4C5-2GateSspGyrB-S11NH2O50.033950.0342A169BI2198, BI2199ok, ok (mut in ccdb E87*)
855
O171O17109-0814:30MaxWGrowth654C1
Qiagen miniprep kit
Plasmid171171pSBX4C5-2GateTerDnaE3-S11CH2O50.024150.0344A171BI2184, BI2185ok, ok
856
O171-2O17109-0814:30MaxWGrowth654C2
Qiagen miniprep kit
Plasmid171171pSBX4C5-2GateTerDnaE3-S11CH2O50.016050.0345discardedA171
857
O172O17209-0814:30MaxWGrowth655C2
Qiagen miniprep kit
Plasmid172172pSBX4C5-2GateTerDnaE3-S11C SN->AGH2O50.017850.0347A172BI2186, BI2187ok, ok
858
O172-2O17209-0814:30MaxWGrowth655C3
Qiagen miniprep kit
Plasmid172172pSBX4C5-2GateTerDnaE3-S11C SN->AGH2O50.017750.0348discardedA172
859
O173O17309-0814:30MaxWGrowth656C1
Qiagen miniprep kit
Plasmid173173pSBX4C5-2GateTerDnaE3-S11NH2O50.07350.0349discardedA173BI2188, BI2189mutation in lacO-reg g->t, part of mrfp+intein not sequenced, part of mrfp+intein not sequenced
860
O173-2O17309-0814:30MaxWGrowth656C2
Qiagen miniprep kit
Plasmid173173pSBX4C5-2GateTerDnaE3-S11NH2O50.09550.0350A173BI2200, BI2201, BI1921ok, ok, ok
861
O174O17409-0814:30MaxWGrowth657C1
Qiagen miniprep kit
Plasmid174174pSBX4C5-2GateTerDnaE3-S11N C->GH2O50.022850.0352discardedA174BI2190, BI2191no mutations, begins in ccdb --> not fully seq, mut. in ccdb ga->tc
862
O174-2O17409-0814:30MaxWGrowth657C2
Qiagen miniprep kit
Plasmid174174pSBX4C5-2GateTerDnaE3-S11N C->GH2O50.012850.0353A174BI2202, BI2203, BI1922ok, ok, ok
863
O143O14309-0814:30MaxWGrowth658C1
Qiagen miniprep kit
Plasmid143143pSBX4C5-2GateNpuDnaE(N)*H2O50.019050.0355discardedA143BI2192, BI2193part of intein/mrfp not sequenced, part of intein/mrfp not sequenced
864
O143-2O14309-0814:30MaxWGrowth658C2
Qiagen miniprep kit
Plasmid143143pSBX4C5-2GateNpuDnaE(N)*H2O50.014150.0356A143BI2204, BI2205, BI1923ok, ok, ok
865
O144O14409-0814:30MaxWGrowth659C1
Qiagen miniprep kit
Plasmid144144pSBX4C5-2GateNpuDnaE(C)*H2O50.020750.0358discardA144
866
O144-2O14409-0814:30MaxWGrowth659C2
Qiagen miniprep kit
Plasmid144144pSBX4C5-2GateNpuDnaE(C)*H2O50.018148.0359A144BI2206, BI2207ok, ok
867
P264P26409-0914:00LisaPCR1201
PCR purification kit
PP264pSBX1C3-Ter_KpnI_Ext_IntN_BamHIH2O50.08528.2
868
P263P26309-0913:00CaroPCR1200
PCR purification kit
PP263180sfGFP_T65C_H2030.030.0
869
R238R23809-0915:00LisaRestriction238
PCR purification kit
Restriction
238pSBX1C3-Ter_KpnI_Ext_IntN_BamHI (BamHI/K)H2O50.02040.8
870
O112-5O11209-0918:00ConstiPurificationO112-2DilutionPlasmid112112pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_HisH2O350.01348.0
871
P138-4P13809-0921:30ConstiGel284-2, 284-3Gel extractionPP138Xyla_BB_RBS34_X_SP0.0
872
P217-2P21709-0923:00AnnaPCR1215
PCR purification kit
PP217BsaI=GGGT-G4-DNMT1-731-1602-Flag-CTTC=BsaIH2O20.03320.0
873
P147-2P14709-0923:00AnnaPCR1216
PCR purification kit
PP147mDNMT1(731-1602)_BsaI=GGGT-GGG_CGCA=BsaIH2O20.04620.0
874
R239R23909-090:00ConstiRestriction239
PCR purification kit
Restriction
239Xyla_BB_RBS34_X_SP (X/P)H2O20.02014.6
conc. estimated, nanodrop measured 27.3
875
R240R24009-090:00ConstiGel285-2, 285-3Gel extraction
Restriction
240pSBX1K3-BBa_I13507 (X/P)H2O30.01420.4
conc. estimated, nanodrop measured 19.9
876
O170O17009-1017:30MaxWGrowth684C6
Qiagen miniprep kit
Plasmid170170pSBX4C5-2GateSspGyrB-S11N C->GH2O50.019150.0367A170#2BI2208, BI2209, BI1920ok, ok, ok
877
O170-2O17009-1017:30MaxWGrowth684C7
Qiagen miniprep kit
Plasmid170170pSBX4C5-2GateSspGyrB-S11N C->GH2O50.019950.0368discardedA170#2
878
O170-3O17009-1017:30MaxWGrowth684C8
Qiagen miniprep kit
Plasmid170170pSBX4C5-2GateSspGyrB-S11N C->GH2O50.022450.0369discardedA170#2
879
O135O13509-1017:30MaxWGrowth685C6
Qiagen miniprep kit
Plasmid135135pSBX4C5-2GateGp41-1(N)H2O50.012050.0370yA135b#2BI2210, BI2211, BI1924ok, ok, ok
880
O135-2O13509-1017:30MaxWGrowth685C7
Qiagen miniprep kit
Plasmid135135pSBX4C5-2GateGp41-1(N)H2O50.01730371discardedA135b#2
881
O135-3O13509-1017:30MaxWGrowth685C8
Qiagen miniprep kit
Plasmid135135pSBX4C5-2GateGp41-1(N)H2O50.01620372discardedA135b#2
882
O136O13609-1017:30MaxWGrowth686C6
Qiagen miniprep kit
Plasmid136136pSBX4C5-2GateGp41-1(C)H2O50.016650.0373yA136b#2BI2212, BI2213ok, ok
883
O136-2O13609-1017:30MaxWGrowth686C7
Qiagen miniprep kit
Plasmid136136pSBX4C5-2GateGp41-1(C)H2O50.017450.0374discardedA136b#2
884
O136-3O13609-1017:30MaxWGrowth686C8
Qiagen miniprep kit
Plasmid136136pSBX4C5-2GateGp41-1(C)H2O50.013050.0375discardedA136b#2
885
O180O18009-1018:40CaroGrowth688C1
Qiagen miniprep kit
Plasmid180180pSBX1K3-T7RBS-H6-sfGFP_T65C-H6H2O30.023630.0discardedP263BI1915, BI1916deletion in 1st Stop codon, deletion in 1st Stop codon
886
R242R24209-1018:40CaroGel292-2,292-3Gel extraction
Restriction
242pSBX4C5-H6-NpuC-mRFP155-225-H6 (X/P)17-3.4
887
R244R24409-1018:40CaroGel292-4,292-5Gel extraction
Restriction
244pSBX4C5-H6-NpuC-mRFP169-225-H6 (X/P)12-1.6
888
R246R24609-1018:40CaroGel292-6,292-7Gel extraction
Restriction
246pSBX4C5-H6-NpuC-RenLucC-H6 (X/P)28-1.2
889
R248R24809-1018:40CaroGel292-9,292-10Gel extraction
Restriction
248pSBX4C5-H6-NpuC-FireLucC-H6 (X/P)8-4.9
890
R250R25009-1018:40CaroGel292-11, 292-12Gel extraction
Restriction
250pSBX4C5-H6-NpuC-mCherryC-H6 (X/P)10-2.7
891
R252R25209-1018:40CaroGel292-13,292-14Gel extraction
Restriction
252pSBX4C5-H6-GFPc158-238-NpuC-H6 (X/P)15-2.1
892
R241R24109-1018:40CaroGel287-2,287-3Gel extraction
Restriction
241pSBX4C5-H6-mRFPn1-154-NpuN-H6 (S/P)14-9.6
893
R243R24309-1018:40CaroGel287-4,287-5Gel extraction
Restriction
243pSBX4C5-H6-mRFP1-168-NpuN-H6 (S/P)7-15.4
894
R245R24509-1018:40CaroGel287-6,287-7Gel extraction
Restriction
245pSBX4C5-H6-RenLucN-NpuN-H6 (S/P)14-10.8
895
R247R24709-1018:40CaroGel287-9, 287-10Gel extraction
Restriction
247pSBX4C5-H6-FireLucN-NpuN-H6 (S/P)19-8.1
896
R249R24909-1018:40CaroGel287-11,287-12Gel extraction
Restriction
249pSBX4C5-H6-mCherryN-NpuN-H6 (S/P)13-10.3
897
R251R25109-1018:40CaroGel287-13, 287-14Gel extraction
Restriction
251pSBX4C5-H6-GFPn1-157-NpuN-H6 (S/P)12-10.9
898
O102-8O10209-1022:00MaxWPurificationO102-3DilutionPlasmid102102pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3H2O150.01148.0
899
R259R25909-1022:45MaxWOligos240,241Annealing
Restriction
259H6+exteins-insertH2O100.03100.0
900
R260R26009-1022:45MaxWOligos200,201Annealing
Restriction
260flex-linkerH2O100.02100.0
901
P147-3P14709-110:30SilvanGel294-2Gel extractionPP147mDNMT1(731-1602)_BsaI=GGGT-GGG_CGCA=BsaIEB0.0
902
P268P26809-111:30MaxWGel295-2, 295-3Gel extractionPP268LiLaLy_BsaI_IasBH2O20.04120.0
903
P217-3P21709-111:30AnnaGel291-2, 291-3Gel extractionPP217BsaI=GGGT-G4-DNMT1-731-1602-Flag-CTTC=BsaIH2O20.09620.0
904
P147-4P14709-111:30AnnaGel294-2Gel extractionPP147mDNMT1(731-1602)_BsaI=GGGT-GGG_CGCA=BsaIH2O20.02720.0
905
P264-2P26409-1110:30LisaGel296-2, 296-3, 296-4Gel extractionPP264pSBX1C3-Ter_KpnI_Ext_IntN_BamHIH2O50.031-15.6
906
R261R26109-1113:00LisaRestriction261
PCR purification kit
Restriction
261pSBX1C3-Ter_KpnI_Ext_IntN_BamHI (BamHI/K)H2O30.02013.9
907
O181O18109-1116:00ConstiGrowth692C1
Qiagen miniprep kit
Plasmid181181pSBX1K3-XylanaseH2O30.03816.9376S181BI1947ok
908
O181-2O18109-1116:00ConstiGrowth692C2
Qiagen miniprep kit
Plasmid181181pSBX1K3-XylanaseH2O30.03230.0377discardedS181
909
O181-3O18109-1116:00ConstiGrowth692C3
Qiagen miniprep kit
Plasmid181181pSBX1K3-XylanaseH2O30.04030.0378S181BI12214ok
910
O181-4O18109-1116:00ConstiGrowth692C4
Qiagen miniprep kit
Plasmid181181pSBX1K3-XylanaseH2O30.0380379discardedS181
911
O181-5O18109-1116:00ConstiGrowth692C5
Qiagen miniprep kit
Plasmid181181pSBX1K3-XylanaseH2O30.0400380discardedS181
912
O125-10O12509-1118:30SilvanGrowth544C8-1
Qiagen miniprep kit
Plasmid125125pSB1C3-Smt3-2ZSortA-H6 (ccdB)EB50.08350.0381L125#2BI1925, BI1932,
913
O178O17809-1118:30SilvanGrowth676C1
Qiagen miniprep kit
Plasmid178178pSB1C3-H6-0ZSortAEB50.025944.2382A178BI1929, BI1936, AG3767ok, no result, ok
914
O178-2O17809-1118:30SilvanGrowth676C2
Qiagen miniprep kit
Plasmid178178pSB1C3-H6-0ZSortAEB50.027650.0383A178BI1930, BI1937, AG3768ok, no result, ok
915
O178-3O17809-1118:30SilvanGrowth676C3
Qiagen miniprep kit
Plasmid178178pSB1C3-H6-0ZSortAEB50.028850.0384A178BI1931, BI1938, AG3769H9R mutation, no result, ok
916
O177O17709-1118:30SilvanGrowth675C1-1
Qiagen miniprep kit
Plasmid177177pSB1C3-2ZSortA-H6EB50.024950.0385A177BI1926, BI1933,
917
O177-2O17709-1118:30SilvanGrowth675C2-1
Qiagen miniprep kit
Plasmid177177pSB1C3-2ZSortA-H6EB50.025650.0386A177BI1927, BI1934,
918
O177-3O17709-1118:30SilvanGrowth675C3-1
Qiagen miniprep kit
Plasmid177177pSB1C3-2ZSortA-H6EB50.022450.0387A177BI1928, BI1935,
919
O188O18809-1216:00MaxWGrowth705C1
Qiagen miniprep kit
Plasmid188188pSBX1K3-LiLaLy(H6)H2O30.048030388
dont use, wrong stuff, discarded
G188BI1939, BI1940, luciferase??
920
O188-2O18809-1216:00MaxWGrowth705C2
Qiagen miniprep kit
Plasmid188188pSBX1K3-LiLaLy(H6)H2O30.014930389
dont use, probably wrong stuff, discarded
G188
921
O188-3O18809-1216:00MaxWGrowth705C3
Qiagen miniprep kit
Plasmid188188pSBX1K3-LiLaLy(H6)H2O30.046330390
dont use, wrong stuff, discarded
G188BI1941, BI1942, luciferase??
922
O189O18809-1216:00MaxWGrowth706C1
Qiagen miniprep kit
Plasmid188188pSBX1K3-LiLaLy(H6)H2O30.023930391is pSBX1K3-LiLaLy(H6)G189BI1943, BI1944, is pSBX1K3-LiLaLy(H6)
923
O189-2O18809-1216:00MaxWGrowth706C2
Qiagen miniprep kit
Plasmid188188pSBX1K3-LiLaLy(H6)H2O30.020630392is pSBX1K3-LiLaLy(H6)G189BI1945, BI1946, is pSBX1K3-LiLaLy(H6)
924
O189-3O18909-1216:00MaxWGrowth706C3
Qiagen miniprep kit
Plasmid189189pSBX4C5-2Zv2Npu:LaLys+flexlinkerH2O30.025630393
dont use, wrong stuff,discarded
G189
925
O161O16109-1217:30LisaGrowth710C1
Qiagen miniprep kit
Plasmid161161pSB1C3-BBa_K741002-Ter_F_KpnI-Ext_IntN_BamHIH2O50.05846.6394S161#5BI1952hä?
926
O161-2O16109-1217:30LisaGrowth710C2
Qiagen miniprep kit
Plasmid161161pSB1C3-BBa_K741002-Ter_F_KpnI-Ext_IntN_BamHIH2O50.02742.7395S161#5
927
O161-3O16109-1217:30LisaGrowth710C3
Qiagen miniprep kit
Plasmid161161pSB1C3-BBa_K741002-Ter_F_KpnI-Ext_IntN_BamHIH2O50.05646.5396S161#5
928
O182O18209-1218:30CaroGrowth694C1
Qiagen miniprep kit
Plasmid182182pSBX4C5-mRFPn1-154_mRFPc155-255_coexH2O30.068130397S182BI1948, BI1949ok, ok
929
O182-2O18209-1218:30CaroGrowth694C2
Qiagen miniprep kit
Plasmid182182pSBX4C5-mRFPn1-154_mRFPc155-255_coexH2O30.058630398S182BI1950, BI1951ok, ok
930
R265R26509-1223:00CaroGel303-2,303-3Gel extraction
Restriction
265pSB1C3-BBa_E0240 (E/S)H2O30.01020.1
931
R266R26609-1223:00CaroGel303-4,303-5Gel extraction
Restriction
266pSB1C3-BBa_J06702 (E/S)H2O30.0617.9
932
R267R26709-1223:00CaroGel303-6,303-7Gel extraction
Restriction
267pSB4C5-BBa_J04450 (E/S)H2O30.01418.0
933
R276R27609-1318:00LisaGel304-2, 304-3Gel extraction
Restriction
276pSB1C3-BBa_K808000 (E/S)H2O30.012-0.4
934
R268R26809-1319:00LisaOligos271,272Annealing
Restriction
268EX-BsaI-CC-O-IasB-SH2O100.01999.2
935
R269R26909-1319:00LisaOligos273,274Annealing
Restriction
269SsrA-Ctag-BsaI-Cext_BsaIH2O100.019100.0
936
R270R27009-1319:00LisaOligos275,276Annealing
Restriction
270MTS_bs_minD_C_termH2O100.032100.0
937
R271R27109-1319:00LisaOligos277,278Annealing
Restriction
271myristoilation-NtagH2O100.014100.0
938
R272R27209-1319:00LisaOligos279,280Annealing
Restriction
272NLS-NtagH2O100.015100.0
939
R273R27309-1319:00LisaOligos283,284Annealing
Restriction
273pel_B-NtagH2O100.029100.0
940
R274R27409-1319:00LisaOligos285,286Annealing
Restriction
274PKI_NES-NtagH2O100.017100.0
941
R275R27509-1319:00LisaOligos269,270Annealing
Restriction
275EX-BsaI-A-NN-IasB-SH2O100.01999.2
942
O182-3O18209-1322:00CaroGrowth694C4
Qiagen miniprep kit
Plasmid182182pSBX4C5-mRFPn1-154_mRFPc155-255_coexH2O30.058530.0discardedS182BI1972, BI1973ok, ok
943
O182-4O18209-1322:00CaroGrowth694C5
Qiagen miniprep kit
Plasmid182182pSBX4C5-mRFPn1-154_mRFPc155-255_coexH2O30.030.0discardedS182
944
O183O18309-1322:00CaroGrowth695C4
Qiagen miniprep kit
Plasmid183183pSBX4C5-mRFPn1-168-mRFPc169-255_coexH2O30.036530.0S183BI1964, BI1965ok, ok
945
O183-2O18309-1322:00CaroGrowth695C5
Qiagen miniprep kit
Plasmid183183pSBX4C5-mRFPn1-168-mRFPc169-255_coexH2O30.030.0discardedS183
946
O186O18609-1322:00CaroGrowth698C2
Qiagen miniprep kit
Plasmid186186pSBX4C5mCherryN_mCherryC_coexH2O30.046630.0S186BI1966, BI1967ok, ok
947
O186-2O18609-1322:00CaroGrowth698C3
Qiagen miniprep kit
Plasmid186186pSBX4C5mCherryN_mCherryC_coexH2O30.030.0discardedS186
948
O186-3O18609-1322:00CaroGrowth698C4
Qiagen miniprep kit
Plasmid186186pSBX4C5mCherryN_mCherryC_coexH2O30.030.0discardedS186
949
O186-4O18609-1322:00CaroGrowth698C5
Qiagen miniprep kit
Plasmid186186pSBX4C5mCherryN_mCherryC_coexH2O30.030.0discardedS186
950
O187O18709-1322:00CaroGrowth699C1
Qiagen miniprep kit
Plasmid187187pSBX4C5-GFPn1-157_GFPc158-255_coexH2O30.045730.0S187BI1968, BI1969ok, ok
951
O187-2O18709-1322:00CaroGrowth699C2
Qiagen miniprep kit
Plasmid187187pSBX4C5-GFPn1-157_GFPc158-255_coexH2O30.030.0discardedS187
952
O187-3O18709-1322:00CaroGrowth699C3
Qiagen miniprep kit
Plasmid187187pSBX4C5-GFPn1-157_GFPc158-255_coexH2O30.030.0discardedS187
953
O187-4O18709-1322:00CaroGrowth699C4
Qiagen miniprep kit
Plasmid187187pSBX4C5-GFPn1-157_GFPc158-255_coexH2O30.030.0discardedS187
954
O117-5O11709-1423:00JanPurificationO117-3DilutionPlasmid117117pSB1C3-BBa_K741002H20100.0297.0
955
O206O20609-1423:00CaroGrowth726C1
Qiagen miniprep kit
Plasmid206206pSB4C5-GFP_contrH2O30.05137.1S206BI1959, BI1960ok, ok
956
O206-2O20609-1423:00CaroGrowth726C2
Qiagen miniprep kit
Plasmid206206pSB4C5-GFP_contrH2O30.030.0S206
957
O206-3O20609-1423:00CaroGrowth726C3
Qiagen miniprep kit
Plasmid206206pSB4C5-GFP_contrH2O30.030.0S206
958
O205O20509-1423:00CaroGrowth727C1
Qiagen miniprep kit
Plasmid205205pSB4C5-mCherry_contrH2O30.054224.5S205BI1961, BI1962ok, ok
959
O205-2O20509-1423:00CaroGrowth727C2
Qiagen miniprep kit
Plasmid205205pSB4C5-mCherry_contrH2O30.030.0S205
960
O205-3O20509-1423:00CaroGrowth727C3
Qiagen miniprep kit
Plasmid205205pSB4C5-mCherry_contrH2O30.030.0S205
961
P175-2P17509-152:08JanGel312-4, 312-5Gel extractionPP175pSB1C3-BBa_K741002_MT_SspDnaBNH2O20.02018.0
962
O207O20709-1511:00LisaGrowth734C1
Qiagen miniprep kit
Plasmid207207pSB1C3-EX-BsaI-CC-O-IasB-SH2O50.09750.0407S207
963
O207-2O20709-1511:00LisaGrowth734C3
Qiagen miniprep kit
Plasmid208208pSB1C3-EX-BsaI-A-NN-IasB-SH2O50.018550.0408
might be from colony 735C3 instead
S207BI1953, BI1954is O208-2......oo, 3.G afterprefix to AC, is O208-2......oo, 3.G afterprefix to AC
964
O208O20809-1511:00LisaGrowth735C1
Qiagen miniprep kit
Plasmid208208pSB1C3-EX-BsaI-A-NN-IasB-SH2O50.010550.0409S208
965
O208-2O20809-1511:00LisaGrowth735C3
Qiagen miniprep kit
Plasmid207207pSB1C3-EX-BsaI-CC-O-IasB-SH2O50.014050.0410
might be from colony 734C3 instead
S208BI1955, BI1956is O207-2....:(, 3.G afterprefix to AC, is O207-2....:(, 3.G afterprefix to AC
966
P261P26109-1415:00MaggieGel314-2,314-3Gel extractionPP261DNMT1(731-1602)_BsaI=CAAC-CWE_BsaI=CGCAH2O25.04025.0
967
P262P26209-1415:00MaggieGel314-4,314-5Gel extractionPP262ccdB_BsaI=CGCA=BsmBI_BsmBI=ATCA-H5SGRGK-TGCT=BsaIH2O25.07325.0
968
O181-6O18109-1412:00ConstiPurificationO181-3DilutionPlasmid181181pSBX1K3-XylanaseH2O39.0135.0
969
R260-2R26009-1512:15MaxWOligos200,201Annealing
Restriction
260flex-linkerH2O100.02100.0
970
R277R27709-1512:15MaxWOligos244,245Annealing
Restriction
277H6-insert(Xyla)H2O100.02100.0
971
R278R27809-1512:15MaxWOligos246,247Annealing
Restriction
278H6-insert+exteins(Xyla)H2O100.03100.0
972
O179O17909-1417:00AnnaGrowth709C2
Qiagen miniprep kit
Plasmid179179pSBX1K3-T7RBS-Smt3-2ZSortA:(mDNMT1(731-1602)-FLAG)-H6H2O30.045330.0G179#2BI1957, AF9231,
973
O179-2O17909-1417:00AnnaGrowth709C4
Qiagen miniprep kit
Plasmid179179pSBX1K3-T7RBS-Smt3-2ZSortA:(mDNMT1(731-1602)-FLAG)-H6H2O30.028330.0G179#2BI1958, AF9232,
974
O180-2O18009-1518:20AnnaGrowth693C1-1
Qiagen miniprep kit
Plasmid180180pSBX1K3-T7RBS-H6-sfGFP_T65C-H6H2O50.050.0discardedO180BI1970, BI1971deletion in 1st Stop codon, deletion in 1st Stop codon
975
P304P30409-1519:30ConstiGel317-2, 317-3Gel extractionPP304XylaLin_BsmBI_IBmsBH2O20.01820.0
976
P148-2P14809-1421:00AnnaGel319-2, 319-3Gel extractionPP148mRFPexp_BsaI=CGCA=BsmBI_BsmBI=CTTC=BsaIH2O20.016820.0vielleicht 218
977
P218-2P21809-1421:00AnnaGel319-4,319-5Gel extractionPP218CGCA=BsaI-PlacI-mRFP-BsaI=CTTCH2O20.018420.0vielleicht 218
978
O192O19209-171:00CaroGrowth768C1
Qiagen miniprep kit
Plasmid192192pSBX4C5-1GateNpuDnaE(N)*-H64070.0427G192#2BI1980looks like pSBX4C5-1GateNpuDnaEC_SN->AG_H6
979
O193O19309-171:00CaroGrowth769C1
Qiagen miniprep kit
Plasmid193193pSBX4C5-1GateSspDnaX-S11(C)-H6354-2.8428G193#2BI1982ok
980
O194O19409-171:00CaroGrowth770C1
Qiagen miniprep kit
Plasmid194194pSBX4C5-1GateSspDnaX-S11C HN->AG_H64200.0429G194#2BI1984ok
981
O195O19509-171:00CaroGrowth771C1
Qiagen miniprep kit
Plasmid195195pSBX4C5-1GateSspDnaX-S11N_H6370-2.7430G195#2BI1986ok
982
O196O19609-171:00CaroGrowth772C1
Qiagen miniprep kit
Plasmid196196pSBX4C5-1GateSspDnaX-S11N C->G_H63910.0431G196#2BI1988ok
983
O197O19709-171:00CaroGrowth773C1
Qiagen miniprep kit
Plasmid197197pSBX4C5-1GateSspGyrB-S11C_H6408-2.5432G197#2BI1990ok
984
O198O19809-171:00CaroGrowth774C1
Qiagen miniprep kit
Plasmid198198pSBX4C5-1GateSspGyrB-S11C SN->AG_H63630.0433G198#2BI1992ok
985
O199O19909-171:00CaroGrowth775C1
Qiagen miniprep kit
Plasmid199199pSBX4C5-1GateSspGyrB-S11N_H6330-3.0434G199#2BI1994ok
986
O200O20009-171:00CaroGrowth776C1
Qiagen miniprep kit
Plasmid200200pSBX4C5-1GateSspGyrB-S11N C->G_H63120.0435G200#2BI1996ok
987
O201O20109-171:00CaroGrowth777C1
Qiagen miniprep kit
Plasmid201201pSBX4C5-1GateTerDnaE3-S11C_H6432-2.3436G201#2BI1998ok
988
O202O20209-171:00CaroGrowth778C1
Qiagen miniprep kit
Plasmid202202pSBX4C5-1GateTerDnaE3-S11C SN->AG_H63800.0437G202#2BI2000ok
989
O203O20309-171:00CaroGrowth779C1
Qiagen miniprep kit
Plasmid203203pSBX4C5-1GateTerDnaE3-S11(N)-H6459-2.2438G203#2BI2002ok
990
O204O20409-171:00CaroGrowth780C1
Qiagen miniprep kit
Plasmid204204pSBX4C5-1GateTerDnaE3-S11(N)*-H64000.0439G204#2BI2004ok
991
P230-2P23009-171:15SilvanGel323-2,323-3Gel extractionPP230pSB1C3_mockSortAc_H6-Smt3EB15.03310.0
992
P234-2P23409-171:15SilvanGel323-4,323-5Gel extractionPP234pSB1C3_SortAc-H6_TEVc-BsaIEB15.0710.0
993
P232-2P23209-171:15SilvanGel323-6,323-7Gel extractionPP232pSB1C3_mockSortAc_H6-TEVcEB15.0210.0
994
P238-2P23809-171:15SilvanGel323-12,323-13Gel extractionPP238H6Smt3_BBprefix_TEVc-BsaIEB15.08215.0
995
P231-2P23109-171:15SilvanGel324-2,324-3Gel extractionPP231mRFPexp_Smt3-TEVc-BsaI_mockSortAc-BBsuffixEB15.07815.0
996
P233-2P23309-171:15SilvanGel324-4,324-5Gel extractionPP233mRFPexp_H6-TEVc_mockSortAc-BBsuffixEB15.07415.0
997
P235-2P23509-171:15SilvanGel324-6,324-7Gel extractionPP235mRFPexp_TEVc-BsaI_SortAc-H6-BBsuffixEB15.04715.0
998
P230-3P23009-173:50SilvanGel328-2,328-3Gel extractionPP230pSB1C3_mockSortAc_H6-Smt3EB15.06515.0
999
P234-3P23409-173:50SilvanGel328-4,328-5Gel extractionPP234pSB1C3_SortAc-H6_TEVc-BsaIEB15.07815.0
1000
P232-3P23209-173:50SilvanGel328-6,328-7Gel extractionPP232pSB1C3_mockSortAc_H6-TEVcEB15.07415.0
1001
P147-5P14709-174:20SilvanGel329-1,329-3Gel extractionPP147mDNMT1(731-1602)_BsaI=GGGT-GGG_CGCA=BsaIEB20.03820.0
1002
O219O21909-1715:26JanExternDominik NiopekPlasmid219219pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)H20500.01487.0
1003
O162-3O16209-1716:00AnnaGrowth665C5
Qiagen miniprep kit
Plasmid162162pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB)H2O50.031050.0S162#3
1004
O189-4O18909-1716:00MaxWGrowth794C1
Qiagen miniprep kit
Plasmid189189pSBX4C5-2Zv2Npu:LaLys+flexlinkerH2O30.020530.0418discardedG189#2BI1974, BI1975ok, bad seq
1005
O189-5O18909-1716:00MaxWGrowth794C2
Qiagen miniprep kit
Plasmid189189pSBX4C5-2Zv2Npu:LaLys+flexlinkerH2O30.022430.0419G189#2BI1976, BI1977ok, ok
1006
O189-6O18909-1716:00MaxWGrowth794C3
Qiagen miniprep kit
Plasmid189189pSBX4C5-2Zv2Npu:LaLys+flexlinkerH2O30.028430.0420discardedG189#2
1007
O216O21609-1716:00MaxWGrowth795C1
Qiagen miniprep kit
Plasmid216216pSBX1K3-XylaH6BBH2O50.017750.0421G216
1008
O216-2O21609-1716:00MaxWGrowth795C2
Qiagen miniprep kit
Plasmid216216pSBX1K3-XylaH6BBH2O50.014446.5422G216BI1978ok
1009
O216-3O21609-1716:00MaxWGrowth795C3
Qiagen miniprep kit
Plasmid216216pSBX1K3-XylaH6BBH2O50.013550.0423G216
1010
O217O21709-1716:00MaxWGrowth796C1
Qiagen miniprep kit
Plasmid217217pSBX1K3-XylaH6extBBH2O50.017150.0424G217BI1979, BF8279bad seq, bad seq
1011
O217-2O21709-1716:00MaxWGrowth796C2
Qiagen miniprep kit
Plasmid217217pSBX1K3-XylaH6extBBH2O50.013046.1425G217BF8280ok
1012
O217-3O21709-1716:00MaxWGrowth796C3
Qiagen miniprep kit
Plasmid217217pSBX1K3-XylaH6extBBH2O50.014550.0426G217
1013
O191O19109-1718:00CaroGrowth811C1
Qiagen miniprep kit
Plasmid191191pSBX4C5-1GateNpuDnaE(C)*-H6H2O30.031430.0440discardedG191#2BI2006looks like pSBX4C5-1GateNpuDnaEN_C->G_H6
1014
O191-2O19109-1718:00CaroGrowth811C2
Qiagen miniprep kit
Plasmid191191pSBX4C5-1GateNpuDnaE(C)*-H6H2O30.030830.0452discardedG191#2BI2008looks like pSBX4C5-1GateNpuDnaEN_C->G_H6
1015
O180-3O18009-1818:00JakobGrowth688C2-1
Qiagen miniprep kit
Plasmid180180pSBX1K3-T7RBS-H6-sfGFP_T65C-H6EB50.06950.0discardedP263Bl2010mutation in first stop codon as well, mutation in chromophore region, no C but S
1016
O180-4O18009-1818:00JakobGrowth688C3-1
Qiagen miniprep kit
Plasmid180180pSBX1K3-T7RBS-H6-sfGFP_T65C-H6EB50.017150.0discardedP263Bl2011
1017
P323P32309-1815:00LisaPCR1502
PCR purification kit
PP323SSpDnaBC_ccdB-CN_CC-mRFPH2O57.95957.9
1018
P324P32409-1815:00LisaPCR1503
PCR purification kit
PP324SSpDnaBC_ccdB-CN_CC*-mRFPH2O41.64041.6
1019
P71-3P7109-18todoLisaPCR1504
PCR purification kit
PP71pSBX4C5_ccdB-BsmBI_BsaI-mRFP_N0.0
no PCR product
1020
P72-3P7209-18todoLisaPCR1505
PCR purification kit
PP72pSBX4C5_mRFP-BsaI_BsmBI-ccdB_C0.0
1021
R59R59todoLisaPCR273Annealing
Restriction
59pSB1T3 (E/P)H2O50.02050.09/18/2014
1022
R60R60todoLisaPCR274Annealing
Restriction
60pSB1C3-BBa_E0240 (E/P)H2O50.02050.09/18/2014
1023
O209O20909-1818:45MaggieGrowth819C1
Qiagen miniprep kit
Plasmid209209
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
88-1.0464G209
1024
O209-2O20909-1818:45MaggieGrowth819C2
Qiagen miniprep kit
Plasmid209209
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
0.0465G209
1025
O209-3O20909-1818:45MaggieGrowth819C3
Qiagen miniprep kit
Plasmid209209
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
0.0466G209
1026
O209-4O20909-1818:45MaggieGrowth819C4
Qiagen miniprep kit
Plasmid209209
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
122-1.0467G209BI2012, IH6892ok, ccdB missing
1027
O209-5O20909-1818:45MaggieGrowth819C5
Qiagen miniprep kit
Plasmid209209
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
0.0468G209
1028
O209-6O20909-1818:45MaggieGrowth819C6
Qiagen miniprep kit
Plasmid209209
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
0.0469G209BI2013, IH6893really is pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu (source vector), really is pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu (source vector)
1029
O209-7O20909-1818:45MaggieGrowth819C7
Qiagen miniprep kit
Plasmid209209
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
0.0470G209
1030
O209-8O20909-1818:45MaggieGrowth819C8
Qiagen miniprep kit
Plasmid209209
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
50-1.0471G209BI2014, IH6894no result (concentration probably was too low), no result (concentration probably was too low)
1031
O176O17609-1818:45MaggieGrowth818C2
Qiagen miniprep kit
Plasmid176176pSB1C3-H6-Smt3-0ZSortA690.0473A176#2AG3764, IH6895ok, ok
1032
O176-2O17609-1818:45MaggieGrowth818C3
Qiagen miniprep kit
Plasmid176176pSB1C3-H6-Smt3-0ZSortA0.0474A176#2AG3765, IH6896H6R mutation, ok
1033
O176-3O17609-1818:45MaggieGrowth818C7
Qiagen miniprep kit
Plasmid176176pSB1C3-H6-Smt3-0ZSortA88-17.1477A176#2AG3766, IH6897ok, ok
1034
P322P32209-192:53JanPCR1499
PCR purification kit
PP322220pL157_C141T (=O220)H2O20.020.0
1035
P322-2P32209-192:53JanPCR1500
PCR purification kit
PP322220pL157_C141T (=O220)H2O20.020.0
1036
O65-12O65
never
MaxWGrowth835C1
Qiagen maxiprep kit
Plasmid6565pSB1C3-BBa_J04450EB50.050.0zuerst mit 50 eluierenO65-8
1037
O65-13O65
never
MaxWGrowth835C1
Qiagen maxiprep kit
Plasmid6565pSB1C3-BBa_J04450EB350.0350.0dann nochmal mit 350O65-8
1038
O210O21009-1913:00LisaGrowth820C1
Qiagen miniprep kit
Plasmid210210pSB1C3-BsaI-CC-O-IasB-Ssra0.0479G210#2
1039
O210-2O21009-1913:00LisaGrowth820C2
Qiagen miniprep kit
Plasmid210210pSB1C3-BsaI-CC-O-IasB-Ssra0.0480G210#2Bl1963
1040
O210-3O21009-1913:00LisaGrowth820C3
Qiagen miniprep kit
Plasmid210210pSB1C3-BsaI-CC-O-IasB-Ssra0.0481G210#2
1041
O211O21109-1913:00LisaGrowth821C1
Qiagen miniprep kit
Plasmid211211pSB1C3-BsaI-CC-O-IasB-MTS0.0482G211#2
1042
O211-2O21109-1913:00LisaGrowth821C2
Qiagen miniprep kit
Plasmid211211pSB1C3-BsaI-CC-O-IasB-MTS0.0483G211#2AG3770
1043
O211-3O21109-1913:00LisaGrowth821C3
Qiagen miniprep kit
Plasmid211211pSB1C3-BsaI-CC-O-IasB-MTS0.0484G211#2
1044
O212O21209-1913:00LisaGrowth822C1
Qiagen miniprep kit
Plasmid212212pSB1C3-EX-BsaI-A-NN-IasB-myristoilation0.0485G212#2
1045
O212-2O21209-1913:00LisaGrowth822C2
Qiagen miniprep kit
Plasmid212212pSB1C3-EX-BsaI-A-NN-IasB-myristoilation0.0486G212#2AG3771
1046
O212-3O21209-1913:00LisaGrowth822C3
Qiagen miniprep kit
Plasmid212212pSB1C3-EX-BsaI-A-NN-IasB-myristoilation0.0487G212#2
1047
O213O21309-1913:00LisaGrowth823C1
Qiagen miniprep kit
Plasmid213213pSB1C3-EX-BsaI-A-NN-IasB-NLS0.0488G213#2
1048
O213-2O21309-1913:00LisaGrowth823C2
Qiagen miniprep kit
Plasmid213213pSB1C3-EX-BsaI-A-NN-IasB-NLS0.0489G213#2AG3772
1049
O213-3O21309-1913:00LisaGrowth823C3
Qiagen miniprep kit
Plasmid213213pSB1C3-EX-BsaI-A-NN-IasB-NLS0.0490G213#2
1050
O214O21409-1913:00LisaGrowth824C1
Qiagen miniprep kit
Plasmid214214pSB1C3-EX-BsaI-A-NN-IasB-pelB0.0491G214#2
1051
O214-2O21409-1913:00LisaGrowth824C2
Qiagen miniprep kit
Plasmid214214pSB1C3-EX-BsaI-A-NN-IasB-pelB0.0492G214#2AG3773
1052
O214-3O21409-1913:00LisaGrowth824C3
Qiagen miniprep kit
Plasmid214214pSB1C3-EX-BsaI-A-NN-IasB-pelB0.0493G214#2
1053
O215O21509-1913:00LisaGrowth825C1
Qiagen miniprep kit
Plasmid215215pSB1C3-EX-BsaI-A-NN-IasB-PKI_NES0.0494G215#2
1054
O215-2O21509-1913:00LisaGrowth825C2
Qiagen miniprep kit
Plasmid215215pSB1C3-EX-BsaI-A-NN-IasB-PKI_NES0.0495G215#2
1055
O215-3O21509-1913:00LisaGrowth825C3
Qiagen miniprep kit
Plasmid215215pSB1C3-EX-BsaI-A-NN-IasB-PKI_NES0.0496G215#2
1056
O218O21809-1913:00LisaGrowth826C1
Qiagen miniprep kit
Plasmid218218pSB1A2-BBa_K1030060.0497D2012-2-22P
1057
O218-2O21809-1913:00LisaGrowth826C2
Qiagen miniprep kit
Plasmid218218pSB1A2-BBa_K103006H2O50.09050.0498D2012-2-22P
1058
O218-3O21809-1913:00LisaGrowth826C3
Qiagen miniprep kit
Plasmid218218pSB1A2-BBa_K1030060.0499D2012-2-22P
1059
O209-9O20909-1818:00MaggieGrowth819C1
Qiagen miniprep kit
Plasmid209209
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
0.0464G209
1060
O209-10O20909-1818:00MaggieGrowth819C2
Qiagen miniprep kit
Plasmid209209
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
0.0465G209
1061
O209-11O20909-1818:00MaggieGrowth819C3
Qiagen miniprep kit
Plasmid209209
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
0.0466G209
1062
O209-12O20909-1818:00MaggieGrowth819C4
Qiagen miniprep kit
Plasmid209209
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
0.0467G209
1063
O209-13O20909-1818:00MaggieGrowth819C5
Qiagen miniprep kit
Plasmid209209
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
0.0468G209
1064
R284R28409-1918:00JakobGel343-2-1, 343-3-1Gel extraction
Restriction
284pSBX4C5-BBa_I13507 (E/S)30.01616.9
1065
R285R28509-1918:00JakobGel343-4-2, 343-5-2Gel extraction
Restriction
285pSB4C5-mCherry_contr (E/S)30.0920.1
1066
R286R28609-1918:00JakobGel343-6-2, 343-7-2Gel extraction
Restriction
286pSB4C5-GFP_contr (E/S)30.0719.1
1067
O175O17509-1918:00AnnaGrowth829C2
Qiagen miniprep kit
Plasmid175175
pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6
1940.0G175#6BI1985, BI2003ok, ok
1068
O175-2O17509-1918:00AnnaGrowth829C3
Qiagen miniprep kit
Plasmid175175
pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6
1410.0G175#6BI1987, BI2005ok, ok
1069
O175-3O17509-1918:00AnnaGrowth829C5
Qiagen miniprep kit
Plasmid175175
pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6
2090.0G175#6BI1989, BI2007ok, ok
1070
O175-4O17509-1918:00AnnaGrowth831C1
Qiagen miniprep kit
Plasmid175175
pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6
1020.0190G175#7BI1991, BI2009really is BsmBI version; ok, really is BsmBI version; ok
1071
O175-5O17509-1918:00AnnaGrowth832C2
Qiagen miniprep kit
Plasmid175175
pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6
2470.0G175#6BI1993, AF9233ok, ok
1072
O175-6O17509-1918:00AnnaGrowth834C2
Qiagen miniprep kit
Plasmid175175
pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6
3410.0190G175#7BI1995, AF9234really is BsmBI version; ok, really is BsmBI version; ok
1073
O175-7O17509-1918:00AnnaGrowth834C3
Qiagen miniprep kit
Plasmid175175
pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6
880.0190G175#7BI1997, AF9235really is BsmBI version; ok, really is BsmBI version; ok
1074
O190O19009-1918:00AnnaGrowth834C5
Qiagen miniprep kit
Plasmid190190
pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsmBI-mRFPexp-BsmBI=CTTC)-H6
2770.0G175#7BI1999, AF9236ok, ok
1075
O190-2O19009-1918:00AnnaGrowth833C4
Qiagen miniprep kit
Plasmid190190
pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsmBI-mRFPexp-BsmBI=CTTC)-H6
750.0G190#6BI2001, II2292ok, ok
1076
R287R28709-2111:30JakobGel350-2-1, 350-3-1Gel extraction
Restriction
287pSBX4C5-BBa_I13507 (E/P)H2O30.02716.6
1077
R288R28809-2111:30JakobGel350-4-2, 350-5-2Gel extraction
Restriction
288pSB4C5-mCherry_contr (E/P)H2O30.01720.4
1078
R289R28909-2111:30JakobGel350-6-2, 350-7-2Gel extraction
Restriction
289pSB4C5-GFP_contr (E/P)H2O30.01319.1
1079
O62-11O6209-2111:30JakobGrowth327C6-1
Qiagen midiprep kit
Plasmid6262pSBX4C5-BBa_I13507H2O200.0320193.7
not sure if I accidentally swaped tubes. we will see iafter digest
O62
1080
O104-3O10409-2111:30JakobGrowth434C1-7
Qiagen midiprep kit
Plasmid104104pSB1C3-BBa_I746908H2O200.0238191.6
not sure if I accidentally swaped tubes. we will see iafter digest
D2014-4-5C
1081
O223O22309-2112:30JakobGrowth860C1
Qiagen miniprep kit
Plasmid223223pSBX4C5-0GateSspDnaX-S11C_mRFP169-225H2O30.054228.2G223BF8267ok good
1082
O224O22409-2112:30JakobGrowth861C1
Qiagen miniprep kit
Plasmid224224pSBX4C5-0GateSspDnaX-S11N_mRFP1-168H2O30.047225.8G224BF8268ok good
1083
O225O22509-2112:30JakobGrowth862C1
Qiagen miniprep kit
Plasmid225225pSBX4C5-0GateSspGyrB-S11C_mRFP169-225H2O30.057128.2G225BF8270voller mutationen -> falsches FP insert? NEU, use O225-2
1084
O226O22609-2112:30JakobGrowth863C1
Qiagen miniprep kit
Plasmid226226pSBX4C5-0GateSspGyrB-S11N_mRFP1-168H2O30.030426.7
no purification since red color, but growths on old plates with BL21
G226BF8271frameshift mutation? NEU,useO226-4
1085
O227O22709-2112:30JakobGrowth864C1
Qiagen miniprep kit
Plasmid227227pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225H2O30.054428.2G227BF8272kein insert? NEU
1086
O228O22809-2112:30JakobGrowth865C1
Qiagen miniprep kit
Plasmid228228pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168H2O30.087828.9G228BF8273mutation im prefix? but less mutation than O228-2,use O228-4
1087
O229O22909-2112:30JakobGrowth866C1
Qiagen miniprep kit
Plasmid229229pSBX4C5-0GateNpuDnaE_C_mRFP169-225H2O30.052428.1one colony on BL21 plateG229BF8275OK good
1088
O230O23009-2112:30JakobGrowth867C1
Qiagen miniprep kit
Plasmid230230pSBX4C5-0GateNpuDnaE_N_mRFP1-168H2O30.030026.7
no purification since red color, but growths on old plates with BL21
G230BF8276mutation in mRFP G toD
1089
O220O22009-2112:30JakobGrowth843C1
Qiagen miniprep kit
Plasmid220220pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141TH2O30.045230.0508P322
BF8261, BF8262, BF8281, BF8282
, , good, but wrong -> no mutation, good, but wrong -> no mutation
1090
O220-2O22009-2112:30JakobGrowth844C1
Qiagen miniprep kit
Plasmid220220pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141TH2O30.042030.0509P322-2
BF8263, BF8264, BF8283, BF8284
, , ok, good!, ok, good!
1091
O220-3O22009-2112:30JakobGrowth844C2
Qiagen miniprep kit
Plasmid220220pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141TH2O30.042130.0510P322-2
BF8265, BF8266, BF8285, BF8286
, , ok, good!, ok, good!
1092
O228-2O22809-2112:30JakobGrowth853C1
Qiagen miniprep kit
Plasmid228228pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168H2O30.027626.4505G228BF8274mutation im prefix?
1093
O192-2O19209-2112:30JakobGrowth856C1
Qiagen miniprep kit
Plasmid192192pSBX4C5-1GateNpuDnaE(N)*-H6H2O30.039227.5G192#3BF8278ok short sequencing
1094
O191-3O19109-2112:30JakobGrowth857C2
Qiagen miniprep kit
Plasmid191191pSBX4C5-1GateNpuDnaE(C)*-H6H2O30.044127.7507ok, lostG191#3BF8277ok short sequencing
1095
O180-5O18009-210:30JakobGrowth688C4
Qiagen miniprep kit
Plasmid180180pSBX1K3-T7RBS-H6-sfGFP_T65C-H6H2O30.011930.0P263BF8258no mutation has been introduced and frame shift in stop codon
1096
O180-6O18009-210:30JakobGrowth688C5
Qiagen miniprep kit
Plasmid180180pSBX1K3-T7RBS-H6-sfGFP_T65C-H6H2O30.014030.0discardedP263BF8259ok good
1097
O180-7O18009-210:30JakobGrowth688C6
Qiagen miniprep kit
Plasmid180180pSBX1K3-T7RBS-H6-sfGFP_T65C-H6H2O30.014730.0P263BF8260no mutation has been introduced and frame shift in stop codon
1098
P72-4P7209-2216:00LisaPCR1561
PCR purification kit
PP72pSBX4C5_mRFP-BsaI_BsmBI-ccdB_CH2O30.05730.0
1099
P71-4P7109-2216:00LisaPCR1590
PCR purification kit
PP71pSBX4C5_ccdB-BsmBI_BsaI-mRFP_NH2O30.010530.0
1100
O226-2O22609-218:00CaroGrowth851C1
Qiagen miniprep kit
Plasmid226226pSBX4C5-0GateSspGyrB-S11N_mRFP1-1681100.0G226
1101
O230-2O23009-218:00CaroGrowth855C1
Qiagen miniprep kit
Plasmid230230pSBX4C5-0GateNpuDnaE_N_mRFP1-1681210.0G230
1102
O224-2O22409-218:00CaroGrowth861C2
Qiagen miniprep kit
Plasmid224224pSBX4C5-0GateSspDnaX-S11N_mRFP1-168123-8.1G224BF8269ok good
1103
O228-3O22809-218:00CaroGrowth865C2
Qiagen miniprep kit
Plasmid228228pSBX4C5-0GateTerDnaE3-S11N_mRFP1-1682760.0G228
1104
R304R30409-2216:15MaxWOligos369,370Annealing
Restriction
304bad1#1H2O500.04500.0
1105
R305R30509-2216:15MaxWOligos371,372Annealing
Restriction
305bad1#2H2O500.04500.0
1106
R306R30609-2216:15MaxWOligos373,374Annealing
Restriction
306may1#1H2O500.05500.0
1107
R307R30709-2216:15MaxWOligos375,376Annealing
Restriction
307may1#2H2O500.05500.0
1108
R308R30809-2216:15MaxWOligos377,378Annealing
Restriction
308sgt1H2O500.03500.0
1109
R309R30909-2216:15MaxWOligos379,380Annealing
Restriction
309sgt2H2O500.05500.0
1110
R310R31009-2216:15MaxWOligos381,382Annealing
Restriction
310sho1H2O500.01500.0
1111
R311R31109-2216:15MaxWOligos383,384Annealing
Restriction
311sho2H2O500.02500.0
1112
O218-4O21809-2216:30LisaPurificationO218-2DilutionPlasmid218218pSB1A2-BBa_K103006H2O100.0198.0
1113
R298R29809-2219:00CaroGel354-2, 354-3Gel extraction
Restriction
298pSBX4C5-0GateSspDnaX-S11N_mRFP1-168 (E/S)15-6.2
1114
R331R33109-2219:00CaroGel354-4,354-5Gel extraction
Restriction
331pSBX4C5-0GateSspDnaX-S11N_mRFP1-168 (E/S)4-18.5
1115
R299R29909-2219:00CaroGel354-6,354-7Gel extraction
Restriction
299pSBX4C5-0GateSspGyrB-S11N_mRFP1-168 (E/S)10-10.7
1116
R332R33209-2219:00CaroGel354-8,354-9Gel extraction
Restriction
332pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168 (E/S)5-17.3
1117
R300R30009-2219:00CaroGel354-11, 354-12Gel extraction
Restriction
300pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168 (E/S)13-7.6
1118
R303R30309-2219:00CaroGel355-2,355-3Gel extraction
Restriction
303pSBX4C5-1GateNpuDnaE(N)*-H6 (E/S)8-15.2
1119
R294R29409-2219:00CaroRestriction294
PCR purification kit
Restriction
294pSBX4C5-0GateSspDnaX-S11C_mRFP169-225 (E/X)12-15.9
1120
R295R29509-2219:00CaroRestriction295
PCR purification kit
Restriction
295pSBX4C5-0GateSspGyrB-S11C_mRFP169-225 (E/X)7-14.8
1121
R296R29609-2219:00CaroRestriction296
PCR purification kit
Restriction
296pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225 (E/X)13-16.6
1122
R297R29709-2219:00CaroRestriction297
PCR purification kit
Restriction
297pSBX4C5-0GateNpuDnaE_C_mRFP169-225 (E/X)5-17.6
1123
R302R30209-2219:00CaroRestriction302
PCR purification kit
Restriction
302pSBX4C5-1GateNpuDnaE(C)*-H6 (E/X)11-10.3
1124
P261-2P26109-2220:20SilvanGel
356-2-2.6kb,356-3-2.6kb
Gel extractionPP261DNMT1(731-1602)_BsaI=CAAC-CWE_BsaI=CGCAEB30.07230.0
1125
P332P33209-2220:20SilvanGel
356-4-2.6kb,356-5-2.6kb
Gel extractionPP332DNMT1_BsaI=CAAC-CWE_H6SGRGT-TGCT=BsaIEB30.04330.0
1126
P333P33309-2220:20SilvanGel
356-6-2.6kb,356-7-2.6kb
Gel extractionPP333mDNMT1(731-1602)_BsaI=GGGT-G_CGCA=BsaIEB30.06530.0
1127
P218-3P21809-2220:20SilvanGel
356-8-1.1kb,356-9-1.1kb
Gel extractionPP218CGCA=BsaI-PlacI-mRFP-BsaI=CTTCEB30.011130.0
1128
P341P34109-2220:20SilvanGel
356-10-1.1kb,356-11-1.1kb
Gel extractionPP341mRFP_BsmBIBsaI_BsmBI=ATCA-H5SGRGT-TGCT=BsaIEB30.010930.0
1129
P262-2P26209-2220:20SilvanGel
356-12-750bp,356-13-750bp,
Gel extractionPP262ccdB_BsaI=CGCA=BsmBI_BsmBI=ATCA-H5SGRGK-TGCT=BsaIEB30.09830.0
1130
R312R31209-2216:15Max WOligos392,393Annealing
Restriction
312DNMT1lnk_0917/1r_p140.0
1131
R313R31309-2216:15Max WOligos394,395Annealing
Restriction
313DNMT1lnk_0917/1r_p240.0
1132
R314R31409-2216:15Max WOligos396,397Annealing
Restriction
314DNMT1lnk_0917/1r_p340.0
1133
R315R31509-2216:15Max WOligos398,399Annealing
Restriction
315DNMT1lnk_0917/2r_p140.0
1134
R316R31609-2216:15Max WOligos400,401Annealing
Restriction
316DNMT1lnk_0917/2r_p230.0
1135
R317R31709-2216:15Max WOligos402,403Annealing
Restriction
317DNMT1lnk_0917/2r_p330.0
1136
R318R31809-2019:00SilvanOligos265,266Annealing
Restriction
318DNMT1lnk_0825/1r_p150.0
1137
R319R31909-2019:00SilvanOligos267,268Annealing
Restriction
319DNMT1lnk_0825/1r_p260.0
1138
R320R32009-2019:00SilvanOligos263,303Annealing
Restriction
320DNMT1lnk_0901/1f70.0
1139
R321R32109-2019:00SilvanOligos320,312Annealing
Restriction
321DNMT1lnk_0915/1r_p140.0
1140
R322R32209-2019:00SilvanOligos322,323Annealing
Restriction
322DNMT1lnk_0915/1r_p240.0
1141
R323R32309-2019:00SilvanOligos324,325Annealing
Restriction
323DNMT1lnk_0915/1r_p340.0
1142
R324R32409-2019:00SilvanOligos312,313Annealing
Restriction
324DNMT1lnk_0915/2r_p150.0
1143
R325R32509-2019:00SilvanOligos314,315Annealing
Restriction
325DNMT1lnk_0915/2r_p250.0
1144
R326R32609-2019:00SilvanOligos305,306Annealing
Restriction
326DNMT1lnk_0915/3f_p140.0
1145
R327R32709-2019:00SilvanOligos307,308Annealing
Restriction
327DNMT1lnk_0915/3f_p240.0
1146
R328R32809-2019:00SilvanOligos304,311Annealing
Restriction
328DNMT1lnk_0915/4f_p260.0
1147
R329R32909-2019:00SilvanOligos316,317Annealing
Restriction
329DNMT1lnk_0915/6f_p140.0
1148
R330R33009-2019:00SilvanOligos318,319Annealing
Restriction
330DNMT1lnk_0915/6f_p240.0
1149
R301R30109-2220:00CaroRestriction301
PCR purification kit
Restriction
301pSBX4C5-0GateNpuDnaE_N_mRFP1-168 (E/S)8-7.9
1150
O221O22109-2311:00:00JakobGrowth874C1
Qiagen miniprep kit
Plasmid221221pSBX4C5-mCherry_contrH2O30.070330.0S221#2
1151
O222O22209-2311:00:00JakobGrowth876C1
Qiagen miniprep kit
Plasmid222222pSBX4C5-GFP_contrH2O30.094730.0S222#2
1152
P338P33809-2310:00LisaOligos293,295MixPP338SspDnaBN_F_mRFP-NNH2O100.036100.0
1153
P339P33909-2310:00LisaOligos294,295MixPP339SspDnaBN_F_mRFP-NN*H2O100.040100.0
1154
P340P34009-2311:30LisaPCR1591
PCR purification kit
PP340OmpA_linker-BsaI_H2O30.06730.0
1155
O107-3O10709-2314:50JakobPurificationO107DilutionPlasmid107107pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6H2O270.001258.00
1156
O59-13O5909-2314:50JakobPurificationO59-12DilutionPlasmid5959pSBX1K3-BBa_I13507H2O822.801818.80
1157
O225-2O22509-2318:00CaroGrowth862C2
Qiagen miniprep kit
Plasmid225225pSBX4C5-0GateSspGyrB-S11C_mRFP169-225H2O30.069028.6G225BF8287ok,good
1158
O225-3O22509-2318:00CaroGrowth862C3
Qiagen miniprep kit
Plasmid225225pSBX4C5-0GateSspGyrB-S11C_mRFP169-225H2O30.030.0G225BF8288no mRFPc insert
1159
O226-3O22609-2318:00CaroGrowth863C2
Qiagen miniprep kit
Plasmid226226pSBX4C5-0GateSspGyrB-S11N_mRFP1-168H2O30.030.0G226BF8289frame shift in beginning
1160
O226-4O22609-2318:00CaroGrowth863C3
Qiagen miniprep kit
Plasmid226226pSBX4C5-0GateSspGyrB-S11N_mRFP1-168H2O30.044027.7G226BF8290ok, good
1161
O227-2O22709-2318:00CaroGrowth864C2
Qiagen miniprep kit
Plasmid227227pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225H2O30.030.0G227BF8291no mRFPc insert
1162
O227-3O22709-2318:00CaroGrowth864C3
Qiagen miniprep kit
Plasmid227227pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225H2O30.030.0G227BF8292no mRFPc insert
1163
O229-2O22909-2318:00CaroGrowth873C1
Qiagen miniprep kit
Plasmid229229pSBX4C5-0GateNpuDnaE_C_mRFP169-225H2O30.030.0512O229BF8294ok, good
1164
O228-4O22809-2318:00CaroGrowth872C1
Qiagen miniprep kit
Plasmid228228pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168H2O30.066028.5511O228BF8293ok, but one mutation in LacOperon
1165
P342P34209-2322:30JakobGel
360-2-1, 360-3-1, 360-4-1, 360-5-1
Gel extractionPP342pSBX1K3-H6-H6 backboneH2O50.02850.0
1166
P342-2P34209-2322:30JakobGel
360-6-1, 360-7-1, 360-8-1, 360-9-1
Gel extractionPP342pSBX1K3-H6-H6 backboneH2O50.03150.0
1167
P344P34409-2322:30JakobGel360-12-1, 360-13-1Gel extractionPP344261pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha (=261)H2O30.030.0
1168
O223-2O22309-2323:00JakobPurificationO223DilutionPlasmid223223pSBX4C5-0GateSspDnaX-S11C_mRFP169-225H2O572.033572.0is O223 directly diluted
1169
O223-3O22309-2311:00JakobPurificationO223-2DilutionPlasmid223223pSBX4C5-0GateSspDnaX-S11C_mRFP169-225H2O33.00128.00
1170
O244O24409-2413:00MaxWGrowth880C1
Qiagen miniprep kit
Plasmid244244pSBX4C5-2Zv2Npu:LaLys+bad1H2O30.050325.1513G24428HD73ok
1171
O244-2O24409-2413:00MaxWGrowth880C2
Qiagen miniprep kit
Plasmid244244pSBX4C5-2Zv2Npu:LaLys+bad1H2O30.055630.0514G244
1172
O245O24509-2413:00MaxWGrowth881C1
Qiagen miniprep kit
Plasmid245245pSBX4C5-2Zv2Npu:LaLys+may1H2O30.038428.8515G24528HD74ok
1173
O245-2O24509-2413:00MaxWGrowth881C2
Qiagen miniprep kit
Plasmid245245pSBX4C5-2Zv2Npu:LaLys+may1H2O30.048130.0516G245
1174
O246O24609-2413:00MaxWGrowth882C1
Qiagen miniprep kit
Plasmid246246pSBX4C5-2Zv2Npu:LaLys+sgt1H2O30.046529.0517G24628HD75kacke
1175
O246-2O24609-2413:00MaxWGrowth882C2
Qiagen miniprep kit
Plasmid246246pSBX4C5-2Zv2Npu:LaLys+sgt1H2O30.035630.0518G246
1176
O247O24709-2413:00MaxWGrowth883C1
Qiagen miniprep kit
Plasmid247247pSBX4C5-2Zv2Npu:LaLys+sgt2H2O30.049129.1519G24728HD76kacke
1177
O247-2O24709-2413:00MaxWGrowth883C2
Qiagen miniprep kit
Plasmid247247pSBX4C5-2Zv2Npu:LaLys+sgt2H2O30.020430.0520G247
1178
O248O24809-2413:00MaxWGrowth884C1
Qiagen miniprep kit
Plasmid248248pSBX4C5-2Zv2Npu:LaLys+sho1H2O30.062529.3521G24828HD77ok
1179
O248-2O24809-2413:00MaxWGrowth884C2
Qiagen miniprep kit
Plasmid248248pSBX4C5-2Zv2Npu:LaLys+sho1H2O30.042630.0522G248
1180
O249O24909-2413:00MaxWGrowth885C1
Qiagen miniprep kit
Plasmid249249pSBX4C5-2Zv2Npu:LaLys+sho2H2O30.042128.9523G24928HD78ok
1181
O249-2O24909-2413:00MaxWGrowth885C2
Qiagen miniprep kit
Plasmid249249pSBX4C5-2Zv2Npu:LaLys+sho2H2O30.063930.0524G249
1182
P348P34809-243:20JakobGel366-2-1, 366-3-1Gel extractionPP348mRFP169-225_H6-SspDnaXC_H6H2O30.010530.0
1183
P349P34909-243:20JakobGel366-4-1, 366-5-1Gel extractionPP349mRFP169-255_H6-SspDnaXC*_H6H2O30.09430.0
1184
P350P35009-243:20JakobGel366-6-1, 366-7-1Gel extractionPP350mRFP169-225_-6-SspDnaXC-Ext_H6H2O30.08730.0
1185
P351P35109-243:20JakobGel366-8-1, 366-9-1Gel extractionPP351mRFP169-225_-12-SspDnaXC-Ext-Jalpha_H6H2O30.08230.0
1186
P352P35209-243:20JakobGel366-10-1, 366-11-1Gel extractionPP352mRFP169-225_BsaIIasB_H6H2O30.07030.0
1187
R333R33309-244:00SilvanGel368-1,368-2Gel extraction
Restriction
333pSBX1K3-T7RBS_BBv2 (S/P)EB20.03317.7
1188
R334R33409-244:00SilvanGel368-3,368-4Gel extraction
Restriction
334pSB1C3-H6-Smt3-0ZSortA (X/P)EB20.02016.2
1189
R335R33509-244:00SilvanGel368-6,368-7Gel extraction
Restriction
335pSB1C3-H6-0ZSortA (X/P)EB20.01616.2
1190
R336R33609-244:00SilvanGel367-1,367-3Gel extraction
Restriction
336pSB1A3-Smt3-2ZSortA-H6 (ccdB) (E/P)EB20.01916.7
1191
R337R33709-244:00SilvanGel368-8Gel extraction
Restriction
337pSB1C3-BBa_J04450 (E/P)EB20.01116.3
1192
O108-5O10809-249:00PurificationO108-4DilutionPlasmid108108pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6H2O500.01498.0
1193
O105-3O10509-249:00PurificationO105-2DilutionPlasmid105105pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6H2O222.01216.0
1194
O220-4O22009-249:00PurificationO220DilutionPlasmid220220pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141TH2O420.01414.0
1195
O195-2O19509-249:00PurificationO195DilutionPlasmid195195pSBX4C5-1GateSspDnaX-S11N_H6H2O370.01368.0
1196
O106-3O10609-249:00PurificationO106DilutionPlasmid106106pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6H2O210.01208.0
1197
O232O23209-2413:00MaggieGrowth907C1
Qiagen miniprep kit
Plasmid232232pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT)1450.0525G232DD1384, DD1391ok, ok
1198
O232-2O23209-2413:00MaggieGrowth907C2
Qiagen miniprep kit
Plasmid232232pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT)1070.0526G232DD1385, DD1392ok, frameshift mutation behind DNMT1
1199
O231O23109-2413:00MaggieGrowth908C2
Qiagen miniprep kit
Plasmid231231
pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6
790.0527G231DD1386, DD1393,
1200
O231-2O23109-2413:00MaggieGrowth909C1
Qiagen miniprep kit
Plasmid231231
pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6
1020.0528G231
1201
O231-3O23109-2413:00MaggieGrowth909C2
Qiagen miniprep kit
Plasmid231231
pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6
1130.0529G231
1202
O239O23909-2413:00MaggieGrowth910C1
Qiagen miniprep kit
Plasmid239239pSBX1K3-T7RBS-2ZSortA:eGFP-H61240.0530G239
1203
O239-2O23909-2413:00MaggieGrowth910C2
Qiagen miniprep kit
Plasmid239239pSBX1K3-T7RBS-2ZSortA:eGFP-H6138-7.5531G23928HE02ok
1204
O239-3O23909-2413:00MaggieGrowth911C1
Qiagen miniprep kit
Plasmid239239pSBX1K3-T7RBS-2ZSortA:eGFP-H644-7.5532G239DD1387, DD139428HE01no result, no resultok
1205
O239-4O23909-2413:00MaggieGrowth911C2
Qiagen miniprep kit
Plasmid239239pSBX1K3-T7RBS-2ZSortA:eGFP-H6410.0533G239
1206
O233O23309-2413:00MaggieGrowth912C1
Qiagen miniprep kit
Plasmid233233pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP)580.0G233
1207
O233-2O23309-2413:00MaggieGrowth913C1
Qiagen miniprep kit
Plasmid233233pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP)410.0535G233
1208
O233-3O23309-2413:00MaggieGrowth913C2
Qiagen miniprep kit
Plasmid233233pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP)53-18.5536G233DD1388, DD1395no result, no result
1209
O233-4O23309-2413:00MaggieGrowth913C3
Qiagen miniprep kit
Plasmid233233pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP)410.0537G233
1210
O262O26209-2413:00MaggieGrowth917C2
Qiagen miniprep kit
Plasmid262262
pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT)
161-6.0538G262
DD1389, DD139628HE03, 28HE04
no result, no resultok, ok
1211
O262-2O26209-2413:00MaggieGrowth917C3
Qiagen miniprep kit
Plasmid262262
pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT)
1510.0539G262
1212
O262-3O26209-2413:00MaggieGrowth917C4
Qiagen miniprep kit
Plasmid262262
pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT)
1480.0540G262
1213
O262-4O26209-2413:00MaggieGrowth917C5
Qiagen miniprep kit
Plasmid262262
pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT)
2080.0541G262DD1390, DD1397no result, no result
1214
P362P36209-2415:00CaroGel369-2,369-3Gel extractionPP362pSBX1K3-BackboneH61150.0
1215
P363P36309-2415:00CaroGel369-4,369-5Gel extractionPP363SspDnaXc-sfGFP^C-H61040.0
1216
P364P36409-2415:00CaroGel369-6, 369-7Gel extractionPP364SspDnaXc*-sfGFP^C-H61170.0
1217
P365P36509-2415:00CaroGel370-1, 370-2Gel extractionPP365H6-sfGFP^N-DnaX^N-H6 PCR Product290.0
1218
P366P36609-2415:00CaroGel370-3, 370-4Gel extractionPP366H6-sfGFP^N-NpuDnaE^N-H6 C->G PCR Product560.0
1219
P367P36709-2415:00CaroGel370-6, 370-7Gel extractionPP367DnaX-S11N_H6190.0
1220
P368P36809-2515:00CaroGel383-2, 383-3Gel extractionPP368LOV-6-SspDnaXC690.0
1221
P369P36909-2515:00CaroGel383-4, 383-5Gel extractionPP369LOV-12-SspDnaXC340.0
1222
P370P37009-2515:00CaroGel384-1, 384-2, 384-4Gel extractionPP370LOV_H6_BsaIIasB640.0
1223
P371P37109-2515:00CaroGel372-2, 372-3Gel extractionPP371sfGFPC_SspDnaXC_H61110.0
1224
P372P37209-2515:00CaroGel373-2, 373-3Gel extractionPP372sfGFPC_BsaIIasB_H6700.0
1225
O266O266-09-2412:00AnnaExternFloPlasmid266266pLentiCRISPRH2O886.01886.0
1226
O220-5O22009-2415:40PurificationO220-2DilutionPlasmid220220pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141TH2O420.01408.0
1227
O224-3O22409-2415:40PurificationO224DilutionPlasmid224224pSBX4C5-0GateSspDnaX-S11N_mRFP1-168H2O472.01468.0
1228
P382P382-09-2417:30AnnaGel375-2Gel extractionPP382pLentiCRISPR PCR ProductH2O20.0230.0
1229
O260O26009-2418:10JakobGrowth925C1
Qiagen miniprep kit
Plasmid260260pSBX1K3-BBa_I13507-GSGIDECHTH2O30.030.0p1640BF8295insertion, caused by mutated template that was not checked correctly by Jan :-P
1230
O260-2O26009-2418:10JakobGrowth925C2
Qiagen miniprep kit
Plasmid260260pSBX1K3-BBa_I13507-GSGIDECHTH2O30.030.0p1640BF8296insertion Jan
1231
O261O26109-2418:10JakobGrowth926C1
Qiagen miniprep kit
Plasmid261261pSBX1K3-BBa_I13507-GSGIDECHT-JalphaH2O30.030.0p1641BF8297insertion
1232
O261-2O26109-2418:10JakobGrowth926C2
Qiagen miniprep kit
Plasmid261261pSBX1K3-BBa_I13507-GSGIDECHT-JalphaH2O30.030.0p1641DD1398insertion
1233
O260-3O26009-2418:10JakobGrowth927C1
Qiagen miniprep kit
Plasmid260260pSBX1K3-BBa_I13507-GSGIDECHTH2O30.030.0P344DD1399insertion
1234
O260-4O26009-2418:10JakobGrowth927C2
Qiagen miniprep kit
Plasmid260260pSBX1K3-BBa_I13507-GSGIDECHTH2O30.030.0P344DD1400insertion
1235
O255O25509-2418:10JakobGrowth897C1
Qiagen miniprep kit
Plasmid255255pSBX4C5-0GateSspDnaX-coex_mRFP168/169H2O30.030.0S255DD1401, BI2146no , no
1236
O257O25709-2418:10JakobGrowth899C1
Qiagen miniprep kit
Plasmid257257pSBX4C5-0GateTerDnaE3-coex_mRFP168/169H2O30.030.0S257DD1402, BI2194no , no
1237
O259O25909-2418:10JakobGrowth901C1
Qiagen miniprep kit
Plasmid259259pSBX4C5-1GateNpuDnaE_mutated_coexH2O30.030.0S259DD1403, BI2195no , no
1238
O255-2O25509-2418:10JakobGrowth902C1
Qiagen miniprep kit
Plasmid255255pSBX4C5-0GateSspDnaX-coex_mRFP168/169H2O30.030.0S255#2DD1404, DD1406no , no
1239
O257-2O25709-2418:10JakobGrowth903C1
Qiagen miniprep kit
Plasmid257257pSBX4C5-0GateTerDnaE3-coex_mRFP168/169H2O30.030.0S257#2DD1405, DD1407no , no
1240
P345P34509-2420:00JakobGel376-2-1, 376-3-1Gel extractionPP345SspDnaXN_H6H2O30.0630.0
1241
P346P34609-2420:00JakobGel376-4-1, 376-5-1Gel extractionPP346mRFP2-168_H6_SspDnaXNH2O30.010530.0
1242
P347P34709-2420:00JakobGel376-6-1, 376-7-1Gel extractionPP347mRFP2-168_H6_SspDnaXN*H2O30.06730.0
1243
O274O274-09-2420:00AnnaExternJuliaDilutionPlasmid274274PCDna3.1(-)H2O779-1.9
1244
R338R338-09-2420:00AnnaGel377-2Gel extraction
Restriction
338pLentiCRISPR PCR Product (BamHI/HindIII)H2O20.0108.9
1245
R339R339-09-2420:00AnnaGel377-3, 377-4Gel extraction
Restriction
339PCDna3.1(-) (BamHI/HindIII)H2O30.03027.1
1246
P375P37509-250:38JanGel379-1-1, 379-2-1Gel extractionPP375mRFP_BsmBI=GATG=IasB_BsaI=GGTG=IBmsB (C)H2O20.012120.0
1247
P376P37609-250:38JanGel379-3Gel extractionPP376mRFP_BsmBI=TGGT=IasB_BsaI=TAAT=IBmsB (N)H2O20.010920.0
1248
P377P37709-250:38JanGel379-5Gel extractionPP377LacI_VR_pSB*C3H2O20.08320.0
1249
P374P37409-250:38JanPCR1644
PCR purification kit
PP374283pSBX4C5-2GateNpuDnaEC_SN->AG noPstI (=O283)H2O20.016020.0
1250
P325P32509-250:38JanPCR1681
PCR purification kit
PP325SspDnaE(N)_mRFP-NN_NC-ccdBH2O20.021520.0
1251
P326P32609-250:38JanPCR1682
PCR purification kit
PP326SSpDnaE(C)_ccdB-CN_CC-mRFPH2O20.010320.0
1252
P345-2P34509-251:30JakobGel381-2-1, 381-3-1Gel extractionPP345SspDnaXN_H6H2O30.07030.0
1253
O180-8O18009-2512:30JakobGrowth929C1
Qiagen miniprep kit
Plasmid180180pSBX1K3-T7RBS-H6-sfGFP_T65C-H6H2O30.035530.0discardedp1680DD1408no mutagenesis
1254
O180-9O18009-2512:30JakobGrowth929C2
Qiagen miniprep kit
Plasmid180180pSBX1K3-T7RBS-H6-sfGFP_T65C-H6H2O30.019630.0discardedp1680DD1409no mutagenesis
1255
O180-10O18009-2512:30JakobGrowth929C3
Qiagen miniprep kit
Plasmid180180pSBX1K3-T7RBS-H6-sfGFP_T65C-H6H2O30.018124.5p1680DD1410OK, mutagenesis worked
1256
O223-4O22309-2512:30JakobGrowth924C1
Qiagen miniprep kit
Plasmid223223pSBX4C5-0GateSspDnaX-S11C_mRFP169-225H2O30.045030.0128O223-2
1257
R260-3R26009-2515:30MaxWOligos200,201Annealing
Restriction
260flex-linkerH2O500.02500.0
1258
O251O25109-2517:10SilvanGrowth931C2
Qiagen miniprep kit
Plasmid251251pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0917/1r)-H6EB50.047145.0543G251#228HD7996bp deletion
1259
O251-2O25109-2517:10SilvanGrowth931C3
Qiagen miniprep kit
Plasmid251251pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0917/1r)-H6EB50.045.0544G251#228HD8096bp deletion
1260
O252O25209-2517:10SilvanGrowth932C1
Qiagen miniprep kit
Plasmid252252
pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/6f-FLAG)-H6
EB50.040745.0545G252#228HD81mixture
1261
O252-2O25209-2517:10SilvanGrowth932C2
Qiagen miniprep kit
Plasmid252252
pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/6f-FLAG)-H6
EB50.050945.0546G252#228HD82probably ok, but short read; possibly G->V mutation in linker
1262
O253O25309-2517:10SilvanGrowth933C1
Qiagen miniprep kit
Plasmid253253
pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/4r-FLAG)-H6
EB50.048745.0547G253#228HD83ok
1263
O253-2O25309-2517:10SilvanGrowth933C2
Qiagen miniprep kit
Plasmid253253
pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/4r-FLAG)-H6
EB50.045.0548G253#228HD8463bp deletion
1264
O236O23609-2517:10SilvanGrowth934C1
Qiagen miniprep kit
Plasmid236236pSBX1K3-T7RBS-H6-Smt3-0ZSortAEB50.050633.0549S236
28HD85, 28HD97, 28HD98
wrong primer, ok; start of the read (backbone) looks a bit strange, ok
1265
O236-2O23609-2517:10SilvanGrowth934C2
Qiagen miniprep kit
Plasmid236236pSBX1K3-T7RBS-H6-Smt3-0ZSortAEB50.042045.0550S23628HD86wrong primer
1266
O238O23809-2517:10SilvanGrowth935C1
Qiagen miniprep kit
Plasmid238238pSBX1K3-T7RBS-H6-0ZSortAEB50.038132.4551S238
28HD87, 28HD99, 28HE00
wrong primer, ok, ok
1267
O238-2O23809-2517:10SilvanGrowth935C2
Qiagen miniprep kit
Plasmid238238pSBX1K3-T7RBS-H6-0ZSortAEB50.033145.0552S23828HD88wrong primer
1268
O125-11O12509-2517:10SilvanGrowth936C1
Qiagen miniprep kit
Plasmid125125pSB1C3-Smt3-2ZSortA-H6 (ccdB)EB50.031340.0553S12528HD89, 28HD90ok, ok
1269
O125-12O12509-2517:10SilvanGrowth936C2
Qiagen miniprep kit
Plasmid125125pSB1C3-Smt3-2ZSortA-H6 (ccdB)EB50.033350.0554S125
1270
O265O26509-2517:55SilvanGrowth956C1
Qiagen miniprep kit
Plasmid265265pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-0917/1r)-H6EB30.06122.5555G26528HD91single base deletion
1271
O265-2O26509-2517:55SilvanGrowth956C2
Qiagen miniprep kit
Plasmid265265pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-0917/1r)-H6EB30.04922.5556G26528HD92ok
1272
O265-3O26509-2517:55SilvanGrowth956C3
Qiagen miniprep kit
Plasmid265265pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-0917/1r)-H6EB30.08030.0557G265
1273
O250O25009-2517:55SilvanGrowth957C1
Qiagen miniprep kit
Plasmid250250
pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0917/2r-H6SGRGT)
EB30.07022.5558G25028HD94ok
1274
O250-2O25009-2517:55SilvanGrowth957C2
Qiagen miniprep kit
Plasmid250250
pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0917/2r-H6SGRGT)
EB30.022.5559G25028HD95single silent mutation
1275
O254O25409-2517:55SilvanGrowth958C1
Qiagen miniprep kit
Plasmid254254
pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT)
EB30.05822.5560G25428HD96sequence ok; signal intensity decreased after DNMT1
1276
O254-2O25409-2517:55SilvanGrowth958C2
Qiagen miniprep kit
Plasmid254254
pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT)
EB30.08822.5561G25428HD93ok
1277
O254-3O25409-2517:55SilvanGrowth958C3
Qiagen miniprep kit
Plasmid254254
pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT)
EB30.030.0562G254
1278
O65-14O6509-260:30MaxWGrowth835C2
Qiagen miniprep kit
Plasmid6565pSB1C3-BBa_J04450H2O50.0122-7.3O65-8
1279
O65-15O6509-260:30MaxWGrowth835C2
Qiagen miniprep kit
Plasmid6565pSB1C3-BBa_J04450H2O50.0664.8O65-8
1280
O65-16O6509-260:30MaxWGrowth835C2
Qiagen miniprep kit
Plasmid6565pSB1C3-BBa_J04450H2O50.082-11.1O65-8
1281
O65-17O6509-260:30MaxWGrowth835C2
Qiagen miniprep kit
Plasmid6565pSB1C3-BBa_J04450H2O50.07523.2O65-8
1282
O65-18O6509-260:30MaxWGrowth835C2
Qiagen miniprep kit
Plasmid6565pSB1C3-BBa_J04450H2O50.05312.5O65-8
1283
O263O26309-2617:00LisaGrowth946C1
Qiagen miniprep kit
Plasmid263263pSB1C3-EX-BsaI-A-NN-IasB-OmpAH2O50.0156#DIV/0!564G26328HE23, 28HE24,
1284
O263-2O26309-2617:00LisaGrowth946C3
Qiagen miniprep kit
Plasmid263263pSB1C3-EX-BsaI-A-NN-IasB-OmpAH2O50.0232#DIV/0!565G263
DD1411, DD141228HE25, 28HE26
, ok, ok
1285
R340R34009-2620:00MaxWGel
390-2, 390-3, 390-4, 390-5
Gel extraction
Restriction
340pSB1C3-BBa_J04450 (E/P)H2O30.03014.9
1286
R342R34209-2620:00MaxWGel
390-6, 390-7, 390-8, 390-9
Gel extraction
Restriction
342pSB1C3-BBa_J04450 (E/P)H2O30.025-6.9
1287
R344R34409-2620:00MaxWGel
390-10, 390-11, 390-12, 390-13
Gel extraction
Restriction
344pSB1C3-BBa_J04450 (E/P)H2O30.01911.3
1288
R346R34609-2620:00MaxWGel
391-2, 391-3, 391-4, 391-5
Gel extraction
Restriction
346pSB1C3-BBa_J04450 (E/P)H2O30.0159.1
1289
R348R34809-2620:00MaxWGel
391-6, 391-7, 391-8, 391-9
Gel extraction
Restriction
348pSB1C3-BBa_J04450 (E/P)H2O30.01811.2
1290
P419P41909-2621:00CaroGel394-2, 394-3Gel extractionPP419GFPn*1-157_H6_GATG_BsaI_KGG_GGCT=IasB H2O30.06930.0
1291
P420P42009-2621:00CaroGel394-4, 394-5Gel extractionPP420GFPc*158-238_NWC_CAAC_BsaI_H6_TAAT=IasBH2O30.06330.0
1292
P421P42109-2621:00CaroGel394-6, 394-7Gel extractionPP421mCherryN*1-168_BsaI=GATG-H6_KGG-GGCT=IasBH2O30.03130.0
1293
P422P42209-2621:00CaroGel394-9, 394-10Gel extractionPP422mCherryC*169-236_BsaI=AGGC-NWC_H6-TAAT=IasBH2O30.05630.0
1294
P423P42309-2621:00CaroGel394-11, 394-12Gel extractionPP423mRFPn*1-154_H6_BsaI=GATG_KGG-GGCT=IasBH2O30.013930.0
1295
P424P42409-2621:00CaroGel394-13, 394-14Gel extractionPP424mRFPn*1-168_H6_BsaI=GATG_KGG-GGCT=IasBH2O30.013830.0
1296
P425P42509-2621:00CaroGel395-2, 395-3Gel extractionPP425mRFPc*155-225_BsaI=AGGC-NWC_H6_TAAT=IasBH2O30.010530.0
1297
P426P42609-2621:00CaroGel395-4, 395-5Gel extractionPP426mRFPc*169-225_BsaI=AGGC-NWC_H6_TAAT=IasBH2O30.05330.0
1298
O224-4O22409-2621:00CaroGrowth950C2
Qiagen miniprep kit
Plasmid224224pSBX4C5-0GateSspDnaX-S11N_mRFP1-1680.0566O224
1299
O224-5O22409-2621:00CaroGrowth950C3
Qiagen miniprep kit
Plasmid224224pSBX4C5-0GateSspDnaX-S11N_mRFP1-1680.0567O224
1300
O225-4O22509-2621:00CaroGrowth951C2
Qiagen miniprep kit
Plasmid225225pSBX4C5-0GateSspGyrB-S11C_mRFP169-2250.0568O225-3
1301
O225-5O22509-2621:00CaroGrowth951C3
Qiagen miniprep kit
Plasmid225225pSBX4C5-0GateSspGyrB-S11C_mRFP169-2250.0569O225-3
1302
O226-5O22609-2621:00CaroGrowth952C2
Qiagen miniprep kit
Plasmid226226pSBX4C5-0GateSspGyrB-S11N_mRFP1-1680.0570O226-4
1303
O226-6O22609-2621:00CaroGrowth952C3
Qiagen miniprep kit
Plasmid226226pSBX4C5-0GateSspGyrB-S11N_mRFP1-1680.0571O226-4
1304
O230-3O23009-2621:00CaroGrowth953C1
Qiagen miniprep kit
Plasmid230230pSBX4C5-0GateNpuDnaE_N_mRFP1-1680.0572O230
1305
O223-5O22309-2621:00CaroGrowth949C1
Qiagen miniprep kit
Plasmid223223pSBX4C5-0GateSspDnaX-S11C_mRFP169-225253-4.0573O223-2
1306
R350R35009-2621:30MaxWOligos411,412Annealing
Restriction
350univ#1longH2O5002500
1307
R351R35109-2621:30MaxWOligos413,414Annealing
Restriction
351univ#1shortH2O5001500
1308
R352R35209-2621:30MaxWOligos415,416Annealing
Restriction
352univ#3H2O5005500
1309
R353R35309-2621:30MaxWOligos417,418Annealing
Restriction
353bad2#2H2O5001500
1310
R354R35409-2621:30MaxWOligos419,420Annealing
Restriction
354bad2#4H2O5005500
1311
R355R35509-2621:30MaxWOligos421,422Annealing
Restriction
355bad3#2H2O5002500
1312
R356R35609-2621:30MaxWOligos423,424Annealing
Restriction
356bad3#4H2O5003500
1313
R357R35709-2621:30MaxWOligos425,426Annealing
Restriction
357bad4#2H2O5005500
1314
R358R35809-2621:30MaxWOligos427,428Annealing
Restriction
358bad4#4H2O5003500
1315
R359R35909-2621:30MaxWOligos429,430Annealing
Restriction
359bad4#5H2O5004500
1316
R360R36009-2621:30MaxWOligos431,432Annealing
Restriction
360may2#2H2O5004500
1317
R361R36109-2621:30MaxWOligos433,434Annealing
Restriction
361may3#2H2O5005500
1318
R362R36209-2621:30MaxWOligos435,436Annealing
Restriction
362ord1#2H2O5004500
1319
R363R36309-2621:30MaxWOligos437,438Annealing
Restriction
363ord2#2H2O5004500
1320
R364R36409-2621:30MaxWOligos439,440Annealing
Restriction
364ord3#3H2O5004500
1321
R365R36509-2621:30MaxWOligos441,442Annealing
Restriction
365ord4#2H2O5003500
1322
R366R36609-2621:30MaxWOligos443,444Annealing
Restriction
366ord5#2H2O5004500
1323
R367R36709-2621:30MaxWOligos445,446Annealing
Restriction
367ord6#2H2O5004500
1324
R368R36809-2621:30MaxWOligos447,448Annealing
Restriction
368sgt3#2H2O5003500
1325
R369R36909-2621:30MaxWOligos449,450Annealing
Restriction
369sgt4#2H2O5004500
1326
R370R37009-2621:30MaxWOligos451,452Annealing
Restriction
370sho3#2H2O5003500
1327
O191-4O19109-2621:12JanGrowth857C2-2
Qiagen miniprep kit
Plasmid191191pSBX4C5-1GateNpuDnaE(C)*-H6H2O50.049248.0G191#3
1328
R371R37109-2621:30MaxWOligos453,454Annealing
Restriction
371rigid_RGTH2O500.04500.0
1329
O23-4O23AnnaPurificationO23DilutionPlasmid2323pRSFDuet-1-mDNMT1(731-1602)0.0
1330
O23-5O23AnnaPurificationO23DilutionPlasmid2323pRSFDuet-1-mDNMT1(731-1602)0.0
1331
P373P37309-277:16JanPCR1643
PCR purification kit
PP373316pSBX4C5-1GateNpuDnaEC_SN->AG_H6 noPstI (=316)H2O20.020.0
1332
O284O28409-270:30MaxWGrowth965C1
Qiagen miniprep kit
Plasmid284284pSBX1K3-2Zv2Npu:LaLys+bad1H2O50.026647.5574G28428HE49no insert
1333
O284-2O28409-270:30MaxWGrowth965C2
Qiagen miniprep kit
Plasmid284284pSBX1K3-2Zv2Npu:LaLys+bad1H2O50.020850.0575G284
1334
O285O28509-270:30MaxWGrowth966C1
Qiagen miniprep kit
Plasmid285285pSBX1K3-2Zv2Npu:LaLys+may1H2O50.019147.5576G28528HE50ok
1335
O285-2O28509-270:30MaxWGrowth966C2
Qiagen miniprep kit
Plasmid285285pSBX1K3-2Zv2Npu:LaLys+may1H2O50.023450.0577G285
1336
O286O28609-270:30MaxWGrowth967C1
Qiagen miniprep kit
Plasmid286286pSBX1K3-2Zv2Npu:LaLys+sgt1H2O50.020047.5578G28628HE51no insert
1337
O286-2O28609-270:30MaxWGrowth967C2
Qiagen miniprep kit
Plasmid286286pSBX1K3-2Zv2Npu:LaLys+sgt1H2O50.017850.0579G286
1338
O287O28709-270:30MaxWGrowth968C1
Qiagen miniprep kit
Plasmid287287pSBX1K3-2Zv2Npu:LaLys+sgt2H2O50.022447.5580G28728HE52ok
1339
O287-2O28709-270:30MaxWGrowth968C2
Qiagen miniprep kit
Plasmid287287pSBX1K3-2Zv2Npu:LaLys+sgt2H2O50.022050.0581G287
1340
O288O28809-270:30MaxWGrowth969C1
Qiagen miniprep kit
Plasmid288288pSBX1K3-2Zv2Npu:LaLys+sho1H2O50.025247.5582G28828HE53ok
1341
O288-2O28809-270:30MaxWGrowth969C2
Qiagen miniprep kit
Plasmid288288pSBX1K3-2Zv2Npu:LaLys+sho1H2O50.023550.0583G288
1342
O289O28909-270:30MaxWGrowth970C1
Qiagen miniprep kit
Plasmid289289pSBX1K3-2Zv2Npu:LaLys+sho2H2O50.025747.5584G28928HE54ok
1343
O289-2O28909-270:30MaxWGrowth970C2
Qiagen miniprep kit
Plasmid289289pSBX1K3-2Zv2Npu:LaLys+sho2H2O50.019750.0585G289
1344
O290O29009-270:30MaxWGrowth971C1
Qiagen miniprep kit
Plasmid290290pSBX1K3-2Zv2Npu:LaLys+flexlinkerH2O50.022947.5586G29028HE55ok
1345
O290-2O29009-270:30MaxWGrowth971C2
Qiagen miniprep kit
Plasmid290290pSBX1K3-2Zv2Npu:LaLys+flexlinkerH2O50.022050.0587G290
1346
O283O28309-282:24JanGrowth961C2
Qiagen miniprep kit
Plasmid283283pSBX4C5-2GateNpu*(C)_v2H2O50.017949.0591P374
1347
O283-2O28309-282:24JanGrowth961C3
Qiagen miniprep kit
Plasmid283283pSBX4C5-2GateNpu*(C)_v2H2O50.015449.0592P374
1348
O283-3O28309-282:24JanGrowth961C4
Qiagen miniprep kit
Plasmid283283pSBX4C5-2GateNpu*(C)_v2 50.020949.0593P374
1349
P362-2P36209-2815:00CaroPCR1852
PCR purification kit
PP362pSBX1K3-BackboneH6H2O30.02330.0
1350
P72-5P7209-2818:00LisaPCR1853
PCR purification kit
PP72pSBX4C5_mRFP-BsaI_BsmBI-ccdB_C30.010630.0
1351
P71-5P7109-2818:00LisaPCR1855
PCR purification kit
PP71pSBX4C5_ccdB-BsmBI_BsaI-mRFP_N30.08430.0
1352
O291O29109-2916:00MaxWGrowth1006C1
Qiagen miniprep kit
Plasmid291291pSBX1K3-2Zv2Npu:LaLys+bad2H2O50.020047.5636G29128HE56bad
1353
O291-2O29109-2916:00MaxWGrowth1006C2
Qiagen miniprep kit
Plasmid291291pSBX1K3-2Zv2Npu:LaLys+bad2H2O50.020050.0637G291
1354
O292O29209-2916:00MaxWGrowth1007C1
Qiagen miniprep kit
Plasmid292292pSBX1K3-2Zv2Npu:LaLys+bad3H2O50.020047.5638G29228HE57bad
1355
O292-2O29209-2916:00MaxWGrowth1007C2
Qiagen miniprep kit
Plasmid292292pSBX1K3-2Zv2Npu:LaLys+bad3H2O50.020050.0639G292
1356
O293O29309-2916:00MaxWGrowth1008C1
Qiagen miniprep kit
Plasmid293293pSBX1K3-2Zv2Npu:LaLys+bad4H2O50.020047.5640G29328HE58bad
1357
O293-2O29309-2916:00MaxWGrowth1008C2
Qiagen miniprep kit
Plasmid293293pSBX1K3-2Zv2Npu:LaLys+bad4H2O50.020050.0641G293
1358
O294O29409-2916:00MaxWGrowth1009C1
Qiagen miniprep kit
Plasmid294294pSBX1K3-2Zv2Npu:LaLys+may2H2O50.020047.5642G29428HE59no insert
1359
O294-2O29409-2916:00MaxWGrowth1009C2
Qiagen miniprep kit
Plasmid294294pSBX1K3-2Zv2Npu:LaLys+may2H2O50.020050.0643G294
1360
O295O29509-2916:00MaxWGrowth1010C1
Qiagen miniprep kit
Plasmid295295pSBX1K3-2Zv2Npu:LaLys+may3H2O50.020047.5644G29528HE60half insert
1361
O295-2O29509-2916:00MaxWGrowth1010C2
Qiagen miniprep kit
Plasmid295295pSBX1K3-2Zv2Npu:LaLys+may3H2O50.020050.0645G295
1362
O296O29609-2916:00MaxWGrowth1011C1
Qiagen miniprep kit
Plasmid296296pSBX1K3-2Zv2Npu:LaLys+ord1H2O50.020050.0646G296
1363
O296-2O29609-2916:00MaxWGrowth1011C2
Qiagen miniprep kit
Plasmid296296pSBX1K3-2Zv2Npu:LaLys+ord1H2O50.020047.5647G29628HE61ok
1364
O297O29709-2916:00MaxWGrowth1012C1
Qiagen miniprep kit
Plasmid297297pSBX1K3-2Zv2Npu:LaLys+ord2H2O50.020047.5648G29728HE62deletions
1365
O297-2O29709-2916:00MaxWGrowth1012C2
Qiagen miniprep kit
Plasmid297297pSBX1K3-2Zv2Npu:LaLys+ord2H2O50.020050.0649G297
1366
O298O29809-2916:00MaxWGrowth1013C1
Qiagen miniprep kit
Plasmid298298pSBX1K3-2Zv2Npu:LaLys+ord3H2O50.020047.5650G29828HE63ok
1367
O298-2O29809-2916:00MaxWGrowth1013C2
Qiagen miniprep kit
Plasmid298298pSBX1K3-2Zv2Npu:LaLys+ord3H2O50.020050.0651G298
1368
O299O29909-2916:00MaxWGrowth1014C1
Qiagen miniprep kit
Plasmid299299pSBX1K3-2Zv2Npu:LaLys+ord4H2O50.020047.5652G29928HE64half insert
1369
O299-2O29909-2916:00MaxWGrowth1014C2
Qiagen miniprep kit
Plasmid299299pSBX1K3-2Zv2Npu:LaLys+ord4H2O50.020050.0653G299
1370
O300O30009-2916:00MaxWGrowth1015C1
Qiagen miniprep kit
Plasmid300300pSBX1K3-2Zv2Npu:LaLys+ord5H2O50.020047.5654G30028HE65no insert
1371
O300-2O30009-2916:00MaxWGrowth1015C2
Qiagen miniprep kit
Plasmid300300pSBX1K3-2Zv2Npu:LaLys+ord5H2O50.020050.0655G300
1372
O301O30109-2916:00MaxWGrowth1016C2
Qiagen miniprep kit
Plasmid301301pSBX1K3-2Zv2Npu:LaLys+ord6H2O50.020047.5656G30128HE66no insert
1373
O301-2O30109-2916:00MaxWGrowth1016C3
Qiagen miniprep kit
Plasmid301301pSBX1K3-2Zv2Npu:LaLys+ord6H2O50.020050.0657G301
1374
O302O30209-2916:00MaxWGrowth1017C1
Qiagen miniprep kit
Plasmid302302pSBX1K3-2Zv2Npu:LaLys+sgt3H2O50.020047.5658G30228HE67no insert
1375
O302-2O30209-2916:00MaxWGrowth1017C2
Qiagen miniprep kit
Plasmid302302pSBX1K3-2Zv2Npu:LaLys+sgt3H2O50.020050.0659G302
1376
O303O30309-2916:00MaxWGrowth1018C1
Qiagen miniprep kit
Plasmid303303pSBX1K3-2Zv2Npu:LaLys+sgt4H2O50.020047.5660G30328HE68no insert
1377
O303-2O30309-2916:00MaxWGrowth1018C2
Qiagen miniprep kit
Plasmid303303pSBX1K3-2Zv2Npu:LaLys+sgt4H2O50.020050.0661G303
1378
O304O30409-2916:00MaxWGrowth1019C1
Qiagen miniprep kit
Plasmid304304pSBX1K3-2Zv2Npu:LaLys+sho3H2O50.020047.5662G30428HE69no insert
1379
O304-2O30409-2916:00MaxWGrowth1019C2
Qiagen miniprep kit
Plasmid304304pSBX1K3-2Zv2Npu:LaLys+sho3H2O50.020050.0663G304
1380
O305O30509-2916:00MaxWGrowth1020C1
Qiagen miniprep kit
Plasmid305305pSBX1K3-2Zv2Npu:LaLys+rigid_RGTH2O50.020047.5664G30528HE70insertions
1381
O305-2O30509-2916:00MaxWGrowth1020C2
Qiagen miniprep kit
Plasmid305305pSBX1K3-2Zv2Npu:LaLys+rigid_RGTH2O50.020050.0665G305
1382
O192-3O19209-2911:30CaroGrowth856C2-1
Qiagen miniprep kit
Plasmid192192pSBX4C5-1GateNpuDnaE(N)*-H6H2O30.027826.4G192#3
1383
P175-3P17509-292:52JanGel403-4,403-5Gel extractionPP175pSB1C3-BBa_K741002_MT_SspDnaBNEB20.02220.0
1384
P175-4P17509-292:52JanGel403-10,403-11Gel extractionPP175pSB1C3-BBa_K741002_MT_SspDnaBNEB20.04820.0
1385
P187-2P18709-296:00JanOligo185,193MixPP187187CPEC_GFP_Insert_oligoH2O2600.0
1386
O278O27809-2911:30JakobGrowth983C1
Qiagen miniprep kit
Plasmid278278pSBX1K3-DnaXN-sfGFPn-H6H2O30.038722.3595A278DD1413ok one additional His
1387
O278-2O27809-2911:30JakobGrowth983C2
Qiagen miniprep kit
Plasmid278278pSBX1K3-DnaXN-sfGFPn-H6H2O30.040230.0596A278DD1414ok two additional His
1388
O278-3O27809-2911:30JakobGrowth983C3
Qiagen miniprep kit
Plasmid278278pSBX1K3-DnaXN-sfGFPn-H6H2O30.030.0597A278
1389
O277O27709-2911:30JakobGrowth984C1
Qiagen miniprep kit
Plasmid277277pSBX1K3-DnaXN*-sfGFPn-H6H2O30.030.0598A277DD1415ok one additional His+
1390
O277-2O27709-2911:30JakobGrowth984C2
Qiagen miniprep kit
Plasmid277277pSBX1K3-DnaXN*-sfGFPn-H6H2O30.021025.2599A277DD1416okay
1391
O277-3O27709-2911:30JakobGrowth984C3
Qiagen miniprep kit
Plasmid277277pSBX1K3-DnaXN*-sfGFPn-H6H2O30.030.0600A277
1392
O276O27609-2911:30JakobGrowth985C1
Qiagen miniprep kit
Plasmid276276pSBX1K3-SspDnaXc-sfGFP^C-H6H2O30.011721.5601A276DD1417ok? two additional His
1393
O276-2O27609-2911:30JakobGrowth985C2
Qiagen miniprep kit
Plasmid276276pSBX1K3-SspDnaXc-sfGFP^C-H6H2O30.030.0602A276DD1418do not use! bad mutations
1394
O276-3O27609-2911:30JakobGrowth985C3
Qiagen miniprep kit
Plasmid276276pSBX1K3-SspDnaXc-sfGFP^C-H6H2O30.030.0603A276
1395
O279O27909-2911:30JakobGrowth987C1
Qiagen miniprep kit
Plasmid279279pSBX1K3-LOV-BsaIIasB-sfGFPcH2O30.046227.8604A279DD1419, DD1435could be O281?? checking with next sequencing, changed sequencing or construct, additional His, could be O281?? checking with next sequencing
1396
O279-2O27909-2911:30JakobGrowth987C2
Qiagen miniprep kit
Plasmid279279pSBX1K3-LOV-BsaIIasB-sfGFPcH2O30.030.0605A279DD1420, DD1436
could be O281?? checking with next sequencing, changed sequencing or construct, additional His, could be O281?? checking with next sequencing, changed sequencing or construct, additional His
1397
O279-3O27909-2911:30JakobGrowth987C3
Qiagen miniprep kit
Plasmid279279pSBX1K3-LOV-BsaIIasB-sfGFPcH2O30.030.0606A279
1398
O280O28009-2911:30JakobGrowth988C1
Qiagen miniprep kit
Plasmid280280pSBX1K3-LOV-12-DnaXC-sfGFPcH2O30.030.0607A280DD1421, DD1437no LOV domain, no LOV domain
1399
O280-2O28009-2911:30JakobGrowth988C2
Qiagen miniprep kit
Plasmid280280pSBX1K3-LOV-12-DnaXC-sfGFPcH2O30.030.0608A280DD1422, DD1438one additional His, frame shift in the end, one additional His, frame shift in the end
1400
O280-3O28009-2911:30JakobGrowth988C3
Qiagen miniprep kit
Plasmid280280pSBX1K3-LOV-12-DnaXC-sfGFPcH2O30.050326.2609A28028HE14, 28HE18ok, good, ok, good
1401
O281O28109-2911:30JakobGrowth989C1
Qiagen miniprep kit
Plasmid281281pSBX1K3-LOV-6-DnaXC-sfGFPcH2O30.047227.9610A281DD1423, DD1439
could be O279?? checking with next sequencing, changed sequencing or construct, additional His, could be O279?? checking with next sequencing, changed sequencing or construct, additional His
1402
O281-2O28109-2911:30JakobGrowth989C2
Qiagen miniprep kit
Plasmid281281pSBX1K3-LOV-6-DnaXC-sfGFPcH2O30.030.0613A267DD1424, DD1440
could be O279?? checking with next sequencing, changed sequencing or construct, additional His, could be O279?? checking with next sequencing, changed sequencing or construct, additional His
1403
O281-3O28109-2911:30JakobGrowth989C3
Qiagen miniprep kit
Plasmid281281pSBX1K3-LOV-6-DnaXC-sfGFPcH2O30.030.0614A267
1404
O267O26709-2911:30JakobGrowth990C1
Qiagen miniprep kit
Plasmid267267pSBX1K3-H6-mRFPn-DnaXN-H6H2O30.030.0615A267DD1425bad read at the beginning, probably frame shift mutations in between
1405
O267-2O26709-2911:30JakobGrowth990C2
Qiagen miniprep kit
Plasmid267267pSBX1K3-H6-mRFPn-DnaXN-H6H2O30.030.0616A268DD1426turned out to be pSBX1K3-sfGFPn-NpuDnaEN
1406
O267-3O26709-2911:30JakobGrowth990C3
Qiagen miniprep kit
Plasmid267267pSBX1K3-H6-mRFPn-DnaXN-H6H2O30.037420.8617A26828HE15ok, good
1407
O268O26809-2911:30JakobGrowth991C1
Qiagen miniprep kit
Plasmid268268pSBX1K3-H6-mRFPn-DnaXN*-H6H2O30.030.0618A268DD1427Intein DnaXN insert not included
1408
O268-2O26809-2911:30JakobGrowth991C2
Qiagen miniprep kit
Plasmid268268pSBX1K3-H6-mRFPn-DnaXN*-H6H2O30.030.0619A269DD1428Intein DnaXN insert not included
1409
O268-3O26809-2911:30JakobGrowth991C3
Qiagen miniprep kit
Plasmid268268pSBX1K3-H6-mRFPn-DnaXN*-H6H2O30.051627.2620A26928HE16ok, good
1410
O269O26909-2911:30JakobGrowth992C1
Qiagen miniprep kit
Plasmid269269pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6H2O30.030.0621A269DD1429, DD1441frame shift between His,
1411
O269-2O26909-2911:30JakobGrowth992C2
Qiagen miniprep kit
Plasmid269269pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6H2O30.025626.1622A270DD1430, DD1442ok one additional His,
1412
O269-3O26909-2911:30JakobGrowth992C3
Qiagen miniprep kit
Plasmid269269pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6H2O30.030.0623A270
1413
O270O27009-2911:30JakobGrowth993C1
Qiagen miniprep kit
Plasmid270270pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6H2O30.026026.2624A270DD1431, DD1443okay,
1414
O270-2O27009-2911:30JakobGrowth993C2
Qiagen miniprep kit
Plasmid270270pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6H2O30.030.0625A271DD1432, DD1444okay, one point mutation in Lac Operator,
1415
O270-3O27009-2911:30JakobGrowth993C3
Qiagen miniprep kit
Plasmid270270pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6H2O30.030.0626A271
1416
O271O27109-2911:30JakobGrowth994C1
Qiagen miniprep kit
Plasmid271271pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6H2O30.030.0627A273DD1433, DD14456 amino acids missing in helixintein construct,
1417
O271-2O27109-2911:30JakobGrowth994C2
Qiagen miniprep kit
Plasmid271271pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6H2O30.030.0628A273DD1434, DD1446LOV domain missing,
1418
O273O27309-2911:30JakobGrowth996C1
Qiagen miniprep kit
Plasmid273273pSBX1K3-H6-DnaXC*-mRFPc-H6H2O30.0110#N/A629A27328HE71is pSBX1K3-H6-DnaXC-mRFPc-H6, mutation H->Q ?, short read
1419
O273-2O27309-2911:30JakobGrowth996C2
Qiagen miniprep kit
Plasmid273273pSBX1K3-H6-DnaXC*-mRFPc-H6H2O30.0#N/A628A27328HE72bad read, end of mRFP okay
1420
O273-3O27309-2911:30JakobGrowth996C3
Qiagen miniprep kit
Plasmid273273pSBX1K3-H6-DnaXC*-mRFPc-H6H2O30.0#DIV/0!629A27328HE17many mutations
1421
O316O31609-2911:23JanGrowth1021C1
Qiagen miniprep kit
Plasmid316316pSBX4C5-H6-1GateNpu*(C)_v2H2O30.039529.0P373
1422
O316-2O31609-2911:23JanGrowth1021C2
Qiagen miniprep kit
Plasmid316316pSBX4C5-H6-1GateNpu*(C)_v2H2O30.026229.0P373
1423
O316-3O31609-2911:23JanGrowth1021C3
Qiagen miniprep kit
Plasmid316316pSBX4C5-H6-1GateNpu*(C)_v2H2O30.026429.0P373
1424
O316-4O31609-2911:23JanGrowth1021C4
Qiagen miniprep kit
Plasmid316316pSBX4C5-H6-1GateNpu*(C)_v2H2O30.023029.0P373
1425
O316-5O31609-2911:23JanGrowth1021C5
Qiagen miniprep kit
Plasmid316316pSBX4C5-H6-1GateNpu*(C)_v2H2O30.036629.0P373
1426
O316-6O31609-2911:23JanGrowth1021C6
Qiagen miniprep kit
Plasmid316316pSBX4C5-H6-1GateNpu*(C)_v2H2O30.0355#N/AP37328HE48ok
1427
O273-4O27309-2918:00CaroGrowth1028C1
Qiagen miniprep kit
Plasmid273273pSBX1K3-H6-DnaXC*-mRFPc-H6H2O30.030.0A273
1428
O273-5O27309-2918:00CaroGrowth1028C2
Qiagen miniprep kit
Plasmid273273pSBX1K3-H6-DnaXC*-mRFPc-H6H2O30.030.0A273
1429
O273-6O27309-2918:00CaroGrowth1028C4
Qiagen miniprep kit
Plasmid273273pSBX1K3-H6-DnaXC*-mRFPc-H6H2O30.030.0A273
1430
O275O27509-2918:00CaroGrowth1029C1
Qiagen miniprep kit
Plasmid275275pSBX1K3-SspDnaXc*-sfGFP^C-H6H2O30.0#N/AA27528HE46turned out to be SspDnaXN-sfGFPN, one additional His
1431
O275-2O27509-2918:00CaroGrowth1029C3
Qiagen miniprep kit
Plasmid275275pSBX1K3-SspDnaXc*-sfGFP^C-H6H2O30.0#N/AA27528HE47turned out to be SspDnaXN-sfGFPN, frame shift in front of His
1432
O275-3O27509-2918:00CaroGrowth1029C4
Qiagen miniprep kit
Plasmid275275pSBX1K3-SspDnaXc*-sfGFP^C-H6H2O30.0#DIV/0!A27528HE20no actually DnaXN-GFPn
1433
R402R40209-3017:00LisaGel408-2Gel extraction
Restriction
402pSBX4C5-2GateNpuDnaE(N) (E/P)H2O30.017.6
1434
R403R40309-3017:00LisaGel408-3Gel extraction
Restriction
403pSBX4C5-2GateNpuDnaE(C) (E/P)H2O30.021.1
1435
R384R38409-3015:00MaxWRestriction384
PCR purification kit
Restriction
384pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 (E/P)H2O20.058.6
1436
R385R38509-3015:00MaxWRestriction385
PCR purification kit
Restriction
385pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6 (E/P)H2O20.048.4
1437
R386R38609-3015:00MaxWRestriction386
PCR purification kit
Restriction
386pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6 (E/P)H2O20.068.9
1438
R387R38709-3015:00MaxWRestriction387
PCR purification kit
Restriction
387pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 (E/P)H2O20.089.4
1439
R388R38809-3015:00MaxWRestriction388
PCR purification kit
Restriction
388pSBX1K3-2Zv2Npu:(LambdaLys-H6) (E/P)H2O20.099.3
1440
R389R38909-3015:00MaxWRestriction389
PCR purification kit
Restriction
389pSBX1K3-2Zv2Npu:(LambdaLys-Rigid) (E/P)H2O20.0109.5
1441
R390R39009-3015:00MaxWRestriction390
PCR purification kit
Restriction
390pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His (E/P)H2O20.0109.5
1442
R391R39109-3015:00MaxWRestriction391
PCR purification kit
Restriction
391pSBX1K3-2Zv2Npu:Xyla_circ_noHis (E/P)H2O20.01911.4
1443
R392R39209-3015:00MaxWRestriction392
PCR purification kit
Restriction
392pSBX1K3-1GateNpuDnaE(N) (E/P)H2O20.079.0
1444
R393R39309-3015:00MaxWRestriction393
PCR purification kit
Restriction
393pSBX1K3-1GateNpuDnaE(C) (E/P)H2O20.089.3
1445
R394R39409-3015:00MaxWRestriction394
PCR purification kit
Restriction
394pSBX1K3-2Zv2Npu:Xyla(H6)destrCext (E/P)H2O20.01110.1
1446
R395R39509-3015:00MaxWRestriction395
PCR purification kit
Restriction
395pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu (E/P)H2O20.0139.8
1447
R396R39609-3015:00MaxWRestriction396
PCR purification kit
Restriction
396pSBX1K3-Xylanase (E/P)H2O20.048.6
1448
R397R39709-3015:00MaxWRestriction397
PCR purification kit
Restriction
397pSBX1K3-LiLaLy(H6) (E/P)H2O20.01210.7
1449
R398R39809-3015:00MaxWRestriction398
PCR purification kit
Restriction
398pSBX1K3-XylaH6BB (E/P)H2O20.01210.5
1450
R399R39909-3015:00MaxWRestriction399
PCR purification kit
Restriction
399pSBX1K3-XylaH6extBB (E/P)H2O20.099.9
1451
O227-4O22709-3018:00CaroGrowth1063C1
Qiagen miniprep kit
Plasmid227227pSBX4C5-0GateTerDnaE3-S11C_mRFP169-2250.0G227#2
1452
O227-5O22709-3018:00CaroGrowth1063C2
Qiagen miniprep kit
Plasmid227227pSBX4C5-0GateTerDnaE3-S11C_mRFP169-22573#N/A691G227#228HE05good, ok
1453
O227-6O22709-3018:00CaroGrowth1063C3
Qiagen miniprep kit
Plasmid227227pSBX4C5-0GateTerDnaE3-S11C_mRFP169-2250.0G227#2
1454
O308O30809-3018:00CaroGrowth1064C1
Qiagen miniprep kit
Plasmid308308pSBX4C5-0GateSspDnaX-S11C*_mRFP*169-225130#N/AG30828HE06NpuDnaEC sequence in front of mRFPc not use
1455
O308-2O30809-3018:00CaroGrowth1064C2
Qiagen miniprep kit
Plasmid308308pSBX4C5-0GateSspDnaX-S11C*_mRFP*169-2250.0G308
1456
O308-3O30809-3018:00CaroGrowth1064C3
Qiagen miniprep kit
Plasmid308308pSBX4C5-0GateSspDnaX-S11C*_mRFP*169-2250.0G308
1457
O309O30909-3018:00CaroGrowth1065C1
Qiagen miniprep kit
Plasmid309309pSBX4C5-0GateSspDnaX-S11N*_mRFP*1-1680.0G309
1458
O309-2O30909-3018:00CaroGrowth1065C2
Qiagen miniprep kit
Plasmid309309pSBX4C5-0GateSspDnaX-S11N*_mRFP*1-168130#N/AG30928HE07no DnaXN*
1459
O309-3O30909-3018:00CaroGrowth1065C3
Qiagen miniprep kit
Plasmid309309pSBX4C5-0GateSspDnaX-S11N*_mRFP*1-1680.0G309
1460
O310O31009-3018:00CaroGrowth1066C1
Qiagen miniprep kit
Plasmid310310pSBX4C5-0GateSspGyrB-S11C*_mRFP*169-225133-15.0680actually O308G31028HE08, 28HE21ok good, but not 310, DnaXC* inside (O308), ok, good but is actually DnaXC*-mRFPc (O308)
1461
O310-2O31009-3018:00CaroGrowth1066C2
Qiagen miniprep kit
Plasmid310310pSBX4C5-0GateSspGyrB-S11C*_mRFP*169-2250.0G310
1462
O310-3O31009-3018:00CaroGrowth1066C3
Qiagen miniprep kit
Plasmid310310pSBX4C5-0GateSspGyrB-S11C*_mRFP*169-2250.0G310
1463
O311O31109-3018:00CaroGrowth1067C1
Qiagen miniprep kit
Plasmid311311pSBX4C5-0GateSspGyrB-S11N*_mRFP*1-168115#DIV/0!681actually O309G31128HE09, 28HE35ok good, but not 312 inside, DnaXN* inside (O309-2), ok, good, but actually DnaXN*-mRFPn (O309)
1464
O311-2O31109-3018:00CaroGrowth1067C2
Qiagen miniprep kit
Plasmid311311pSBX4C5-0GateSspGyrB-S11N*_mRFP*1-1680.0G311
1465
O311-3O31109-3018:00CaroGrowth1067C3
Qiagen miniprep kit
Plasmid311311pSBX4C5-0GateSspGyrB-S11N*_mRFP*1-1680.0G311
1466
O312O31209-3018:00CaroGrowth1068C1
Qiagen miniprep kit
Plasmid312312pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-225#DIV/0!682actually O310G31228HE10, 28HE36ok good, but GyrBC* (O310) inside , ok , good, but actually GyrBC*-mRFPc (O310)
1467
O312-2O31209-3018:00CaroGrowth1068C2
Qiagen miniprep kit
Plasmid312312pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-2250.0G312
1468
O312-3O31209-3018:00CaroGrowth1068C3
Qiagen miniprep kit
Plasmid312312pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-2250.0G312
1469
O313O31309-3018:00CaroGrowth1069C1
Qiagen miniprep kit
Plasmid313313pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-168#DIV/0!683actually O311G31328HE11, 28HE37ok good, but GyrBN* (O311) inside, ok, good, but actually GyrBN*-mRFPn (O311)
1470
O313-2O31309-3018:00CaroGrowth1069C2
Qiagen miniprep kit
Plasmid313313pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-1680.0G313
1471
O313-3O31309-3018:00CaroGrowth1069C3
Qiagen miniprep kit
Plasmid313313pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-1680.0G313
1472
O314O31410-01todoCaroGrowth1070C1
Qiagen miniprep kit
Plasmid314314pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225#DIV/0!G31428HE33not use, actually DnaXN- GFPn
1473
O314-2O31410-01todoCaroGrowth1070C2
Qiagen miniprep kit
Plasmid314314pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225#DIV/0!G31428HE34not use, actually DnaXN- GFPn
1474
O314-3O31410-01todoCaroGrowth1070C3
Qiagen miniprep kit
Plasmid314314pSBX4C5-0GateNpuDnaE_C*_mRFP*169-2250.0G314
1475
O315O31509-30todoCaroGrowth1071C1
Qiagen miniprep kit
Plasmid315315pSBX4C5-0GateNpuDnaE_N*_mRFP*1-1680.0G315
1476
O315-2O31509-30todoCaroGrowth1071C2
Qiagen miniprep kit
Plasmid315315pSBX4C5-0GateNpuDnaE_N*_mRFP*1-1680.0G315
1477
O315-3O31509-30todoCaroGrowth1071C3
Qiagen miniprep kit
Plasmid315315pSBX4C5-0GateNpuDnaE_N*_mRFP*1-1680.0G315
1478
O132-4O13209-3018:00LisaGrowth1055C1
Qiagen miniprep kit
Plasmid132132pSB1C3-Plac-GFP-IntN-MTS-7.3A132#428HE41, 28HE42,
1479
O132-5O13209-3018:00LisaGrowth1055C2
Qiagen miniprep kit
Plasmid132132pSB1C3-Plac-GFP-IntN-MTS-7.3A132#428HE40, 28HE39,
1480
O132-6O13209-3018:00LisaGrowth1055C3
Qiagen miniprep kit
Plasmid132132pSB1C3-Plac-GFP-IntN-MTS0.0A132#4
1481
O340O34009-3020:40LisaLigationS344
PCR purification kit
Plasmid00#N/A120.0
1482
O341O34109-3020:40LisaLigationS345
PCR purification kit
Plasmid00#N/A90.0
1483
R383R38309-3022:10MaxWGel414-2Gel extraction
Restriction
383pSBX4C5-2Zv2Npu (E/P)H2O20.059.5
1484
P197-5P19710-010:05LisaGel415-1,415-2Gel extractionPP197ccdB-pSBX4C5-mRFP(N)_H2O20.06820.0
1485
P198-5P19810-010:05LisaGel415-3,415-4Gel extractionPP198mRFP-pSBX4C5-ccdB-(C)_H2O20.03420.0
1486
P478P47810-010:05LisaGel415-5,415-6Gel extractionPP478ccdB-pSB1C3-mRFP(N)_H2O20.06620.0
1487
P479P47910-010:05LisaGel415-7,415-8Gel extractionPP479mRFP-pSB1C3-ccdB-(C)_H2O20.07720.0
1488
O107-4O10710-0114:00MaxWGrowth529C1-1
Qiagen miniprep kit
Plasmid107107pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6H2O30.024125.9A107
1489
P362-3P36210-0116:00CaroGel417-2, 417-3Gel extractionPP362pSBX1K3-BackboneH6H2O30.0830.0
1490
P362-4P36210-0116:00CaroGel417-4, 417-5Gel extractionPP362pSBX1K3-BackboneH6H2O30.01530.0
1491
P362-5P36210-0116:00CaroGel417-6, 417-7Gel extractionPP362pSBX1K3-BackboneH6H2O30.0930.0
1492
P480P48010-0117:00LisaGel418-2, 418-3Gel extractionPP480CBD_His-BsaI_BsaI-NextH2O30.03430.0
1493
O117-6O11710-0118:00CaroGrowth467C2-1
Qiagen miniprep kit
Plasmid117117pSB1C3-BBa_K741002H2O#DIV/0!
D2013-2-17M
28HE12, 28HE13,
1494
O364O36410-0110JanExtern
Konrad Herbst, pKH38.2
Plasmid364364pSBX4C5-1Gate-NpuDnaE(N)-H6-H6-1GateNpuDnaE(C)H2O5.0183#DIV/0!DD148128HE27, 28HE28ok, but only mRFP sequenced, ok, but only mRFP sequenced
1495
O365O36510-0110JanExtern
Konrad Herbst, pKH39PstI.1
Plasmid365365pSBX4C5-1GateNpuDnaE(N)*-H6-1GateNpuDnaE(C)*-H6H2O5.02090.7DD148228HE29, 28HE30ok, but only mRFP sequenced, ok, but only mRFP sequenced
1496
O366O36610-0110JanExtern
Konrad Herbst, pKH39.2
Plasmid366366pSBX4C5-1GateNpuDnaE(N)*-H6-H6-1GateNpu*(C)_v2H2O5.02010.5DD148328HE31, 28HE32ok, but only mRFP sequenced, ok, but only mRFP sequenced
1497
O327O32710-0118:37Jan, LisaGrowth1072C1
Qiagen miniprep kit
Plasmid327327pSBX4C5-1GateGp41-1(N)-H6H2O50.04929.5630G327DD1447ok
1498
O327-2O32710-0219:37Jan, LisaGrowth1072C2
Qiagen miniprep kit
Plasmid327327pSBX4C5-1GateGp41-1(N)-H6H2O50.09539.5674G327DD1448will be repeated
1499
O328O32810-0320:37Jan, LisaGrowth1073C1
Qiagen miniprep kit
Plasmid328328pSBX4C5-H6-1GateGp41-1(C)H2O50.013042.3631G328DD1449ok
1500
O328-2O32810-0421:37Jan, LisaGrowth1073C2
Qiagen miniprep kit
Plasmid328328pSBX4C5-H6-1GateGp41-1(C)H2O50.014543.1675G328DD1450ok
1501
O329O32910-0522:37Jan, LisaGrowth1074C1
Qiagen miniprep kit
Plasmid329329pSBX4C5-DoubleGateGp41-1(N)H2O50.07937.3632G329DD1451, DD1459ok, not completely sequenced, ok, not completely sequenced
1502
O329-2O32910-0623:37Jan, LisaGrowth1074C2
Qiagen miniprep kit
Plasmid329329pSBX4C5-DoubleGateGp41-1(N)H2O50.05130.2676G329DD1452, DD1460bad sequenzing, bad sequenzing
1503
O330O33010-070:37Jan, LisaGrowth1075C1
Qiagen miniprep kit
Plasmid330330pSBX4C5-DoubleGateGp41-1(C)H2O50.010140.1633G330DD1453, DD1461ok, not completely sequenced, ok, not completely sequenced
1504
O330-2O33010-081:37Jan, LisaGrowth1075C2
Qiagen miniprep kit
Plasmid330330pSBX4C5-DoubleGateGp41-1(C)H2O50.07136.0677G330DD1454, DD1462insertion in the prefix, mutation in mRFP, poor sequenzing, but ok
1505
O331O33110-092:37Jan, LisaGrowth1076C1
Qiagen miniprep kit
Plasmid331331pSBX4C5-DoubleNpuDnaE(N)H2O50.06935.4634G331DD1455, DD1463sequencing not long enough, poor sequenzing could be ok intein not sequenced
1506
O331-2O33110-103:37Jan, LisaGrowth1076C2
Qiagen miniprep kit
Plasmid331331pSBX4C5-DoubleNpuDnaE(N)H2O50.09439.3678G331DD1456, DD1464sequencing not long enough, ok intein not sequenzed
1507
O332O33210-114:37Jan, LisaGrowth1077C1
Qiagen miniprep kit
Plasmid332332pSBX4C5-DoubleGateNpuDnaE(C)H2O50.06233.7635G332DD1457, DD1465sequencing not long enough, intein not sequenced, short sequenzin but ok
1508
O332-2O33210-125:37Jan, LisaGrowth1077C2
Qiagen miniprep kit
Plasmid332332pSBX4C5-DoubleGateNpuDnaE(C)H2O50.06735.1679G332DD1458, DD1466poor sequenzing, especially in the beginningg, but no indication that it was not ok, poor sequrnzing could be ok
1509
R404R40410-0122:15MaxWGel419-2Gel extraction
Restriction
404pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6 (E/P)H2O20.019.2
1510
R405R40510-0122:15MaxWGel419-3Gel extraction
Restriction
405pSBX4C5-1Gate-NpuDnaE(N)-H6 (E/P)H2O20.01013.8
1511
R406R40610-0122:15MaxWGel419-4Gel extraction
Restriction
406pSBX4C5-H6-1GateNpuDnaE(C) (E/P)H2O20.0611.5
1512
R407R40710-0122:15MaxWGel419-5Gel extraction
Restriction
407pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 (E/P)H2O20.0310.4
1513
R408R40810-0122:15MaxWGel419-6Gel extraction
Restriction
408pSBX4C5-1GateSspDnaX-S11(C)-H6 (E/P)H2O20.0712.2
1514
R409R40910-0122:15MaxWGel419-7Gel extraction
Restriction
409pSBX4C5-1GateSspDnaX-S11N_H6 (E/P)H2O20.01014.3
1515
R410R41010-0122:15MaxWGel419-8Gel extraction
Restriction
410pSBX4C5-1GateSspGyrB-S11N_H6 (E/P)H2O20.01114.4
1516
R411R41110-0122:15MaxWGel419-9Gel extraction
Restriction
411pSBX4C5-1GateTerDnaE3-S11C_H6 (E/P)H2O20.0712.9
1517
R412R41210-0122:15MaxWGel419-10Gel extraction
Restriction
412pSBX4C5-1GateTerDnaE3-S11(N)-H6 (E/P)H2O20.01014.3
1518
O272O27209-2911:30CaroGrowth1037C1
Qiagen miniprep kit
Plasmid272272pSBX1K3-H6-DnaXC-mRFPc-H6#N/A684A27228HE44, 28HE19actually pSBX1K3-H6-DnaXC*-mRFPc-H6, construct or sequencing changed?, ok good, but actually O273
1519
O272-2O27209-2911:30CaroGrowth1037C2
Qiagen miniprep kit
Plasmid272272pSBX1K3-H6-DnaXC-mRFPc-H6#N/AA27228HE45actually pSBX1K3-H6-DnaXN-sfGFP-H6,do not use frameshift
1520
O272-3O27209-2911:30CaroGrowth1037C3
Qiagen miniprep kit
Plasmid272272pSBX1K3-H6-DnaXC-mRFPc-H60.0A272
1521
O59-14O5902-1016:00JakobPurificationO59-9DilutionPlasmid5959pSBX1K3-BBa_I13507H2O17.015.0
1522
O240O24002-1015:00LisaGrowth1078C1
Qiagen miniprep kit
Plasmid240240pSBX4C5-2GateSspDnaB(C)1170.0692A240#5DD1467ok
1523
O240-2O24002-1015:00LisaGrowth1078C2
Qiagen miniprep kit
Plasmid240240pSBX4C5-2GateSspDnaB(C)0.0693A240#5
1524
O240-3O24002-1015:00LisaGrowth1078C3
Qiagen miniprep kit
Plasmid240240pSBX4C5-2GateSspDnaB(C)880.0694A240#5DD1468ok (maybe there is something in the beginning of mRFP)
1525
O241O24102-1015:00LisaGrowth1079C1
Qiagen miniprep kit
Plasmid241241pSBX4C5-2GateSspDnaB(C)*0.0695A241#5
1526
O241-2O24102-1015:00LisaGrowth1079C2
Qiagen miniprep kit
Plasmid241241pSBX4C5-2GateSspDnaB(C)*1010.0696A241#5DD1469ok
1527
O241-3O24102-1015:00LisaGrowth1079C3
Qiagen miniprep kit
Plasmid241241pSBX4C5-2GateSspDnaB(C)*1210.0697A241#5DD1470ok
1528
O242O24202-1015:00LisaGrowth1080C1
Qiagen miniprep kit
Plasmid242242pSBX4C5-2GateSspDnaB(N)1270.0698A242#5DD1471perfect mRFP read, call GATC
1529
O242-2O24202-1015:00LisaGrowth1080C2
Qiagen miniprep kit
Plasmid242242pSBX4C5-2GateSspDnaB(N)920.0699A242#5DD1472perfect mRFP read, call GATC
1530
O242-3O24202-1015:00LisaGrowth1080C3
Qiagen miniprep kit
Plasmid242242pSBX4C5-2GateSspDnaB(N)0.0700A242#5
1531
O243O24302-1015:00LisaGrowth1081C1
Qiagen miniprep kit
Plasmid243243pSBX4C5-2GateSspDnaB(N)*0.0701A243#5
1532
O243-2O24302-1015:00LisaGrowth1081C2
Qiagen miniprep kit
Plasmid243243pSBX4C5-2GateSspDnaB(N)*680.0702A243#5DD1473perfect mRFP read, call GATC
1533
O243-3O24302-1015:00LisaGrowth1081C3
Qiagen miniprep kit
Plasmid243243pSBX4C5-2GateSspDnaB(N)*500.0703A243#5DD1474perfect mRFP read, call GATC
1534
O325O32502-1015:00LisaGrowth1082C1
Qiagen miniprep kit
Plasmid325325pSBX4C5-2GateSspDnaE(N)800.0704A325#2DD1475, perfect mRFP read, call GATC,
1535
O325-2O32502-1015:00LisaGrowth1082C2
Qiagen miniprep kit
Plasmid325325pSBX4C5-2GateSspDnaE(N)650.0705A325#2DD1476, perfect mRFP read, call GATC,
1536
O325-3O32502-1015:00LisaGrowth1082C3
Qiagen miniprep kit
Plasmid325325pSBX4C5-2GateSspDnaE(N)0.0706A325#2
1537
O326O32602-1015:00LisaGrowth1083C1
Qiagen miniprep kit
Plasmid326326pSBX4C5-2GateSspDnaE(C)950.0707A326#2DD1477double insert insertion
1538
O326-2O32602-1015:00LisaGrowth1083C2
Qiagen miniprep kit
Plasmid326326pSBX4C5-2GateSspDnaE(C)0.0708A326#2
1539
O326-3O32602-1015:00LisaGrowth1083C3
Qiagen miniprep kit
Plasmid326326pSBX4C5-2GateSspDnaE(C)910.0709A326#2DD1478tripple insert insertion
1540
O346O34602-1018:00LisaGrowth1090C2
Qiagen miniprep kit
Plasmid346346pSB1C3-EX-BsaI-CBD0.0711G346DD1479no.....GG could not work
1541
O346-2O34602-1018:00LisaGrowth1090C4
Qiagen miniprep kit
Plasmid346346pSB1C3-EX-BsaI-CBD0.0713G346DD1480no.....GG could not work
1542
O346-3O34602-1018:00LisaGrowth1090C5
Qiagen miniprep kit
Plasmid346346pSB1C3-EX-BsaI-CBD#DIV/0!714G34628HE43no.....GG could not work
1543
O364-2O36402-1023:45MaxWGrowthKH38.2
Qiagen miniprep kit
Plasmid364364pSBX4C5-1Gate-NpuDnaE(N)-H6-H6-1GateNpuDnaE(C)H2O30.020125.0671
1544
O365-2O36502-1023:45MaxWGrowthKH39PstI.1
Qiagen miniprep kit
Plasmid365365pSBX4C5-1GateNpuDnaE(N)*-H6-1GateNpuDnaE(C)*-H6H2O30.013430.0672
1545
O366-2O36602-1023:45MaxWGrowthKH39.2
Qiagen miniprep kit
Plasmid366366pSBX4C5-1GateNpuDnaE(N)*-H6-H6-1GateNpu*(C)_v2H2O30.037227.3673
1546
O260-5O26010-039:30JakobPCR1979
PCR purification kit
Plasmid260260pSBX1K3-BBa_I13507-GSGIDECHTH2O15.015.0
1547
O261-3O26110-039:30JakobPCR1980
PCR purification kit
Plasmid261261pSBX1K3-BBa_I13507-GSGIDECHT-JalphaH2O15.015.0
1548
R61-4R6110-0422:15MaxWOligos37,38Annealing
Restriction
61T7promH2O50.040049.5
1549
R416R41610-0422:15MaxWGel426-2Gel extraction
Restriction
416pSB1C3-BBa_J04450 (E/S)H2O20.050.1
1550
R417R41710-0422:15MaxWGel426-3Gel extraction
Restriction
417pSBX1K3-BBa_I13507 (E/S)H2O20.02014.3
1551
R418R41810-0422:15MaxWGel426-4Gel extraction
Restriction
418pSBX4C5-2Zv2Npu:LaLys+bad1 (E/S)H2O20.0812.9
1552
R419R41910-0422:15MaxWGel426-5Gel extraction
Restriction
419pSBX4C5-1GateNpuDnaE(N)*-H6 (E/P)H2O20.0711.4
1553
R420R42010-0422:15MaxWGel426-6Gel extraction
Restriction
420pSBX4C5-1GateSspGyrB-S11C_H6 (E/P)H2O20.0812.8
1554
R421R42110-0422:15MaxWGel426-7Gel extraction
Restriction
421pSBX4C5-H6-1GateNpu*(C)_v2 (E/P)H2O20.0712.1
1555
R422R42210-0423:15MaxWGel427-2Gel extraction
Restriction
422pSBX4C5-1Gate-NpuDnaE(N)-H6-H6-1GateNpuDnaE(C) (E/P)H2O20.059.5
1556
R423R42310-0423:15MaxWGel427-3Gel extraction
Restriction
423pSBX4C5-1GateNpuDnaE(N)*-H6-H6-1GateNpu*(C)_v2 (E/P)H2O20.059.3
1557
O347O34710-042:00MaxWGrowth1097C1
Qiagen miniprep kit
Plasmid347347pSB1C3-2Zv2NpuH2O50.028450.0717S347
1558
O348O34810-042:00MaxWGrowth1098C1
Qiagen miniprep kit
Plasmid348348pSB1C3-T7RBS-LambdaLys-IGEMHD_v3H2O50.016350.0718S348
1559
O349O34910-042:00MaxWGrowth1099C1
Qiagen miniprep kit
Plasmid349349pSB1C3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6H2O50.022250.0719S349
1560
O350O35010-042:00MaxWGrowth1100C1
Qiagen miniprep kit
Plasmid350350pSB1C3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6H2O50.021050.0720S350
1561
O351O35110-042:00MaxWGrowth1101C1
Qiagen miniprep kit
Plasmid351351pSB1C3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6H2O50.023150.0721S351
1562
O352O35210-042:00MaxWGrowth1102C1
Qiagen miniprep kit
Plasmid352352pSB1C3-2Zv2Npu:(LambdaLys-H6)H2O50.022350.0722S352
1563
O353O35310-042:00MaxWGrowth1103C1
Qiagen miniprep kit
Plasmid353353pSB1C3-2Zv2Npu:(LambdaLys-Rigid)H2O50.022850.0723S353
1564
O354O35410-042:00MaxWGrowth1104C1
Qiagen miniprep kit
Plasmid354354pSB1C3-2Zv2Npu:Xyla_BsaI_circ_HisH2O50.027250.0724S354
1565
O355O35510-042:00MaxWGrowth1105C2
Qiagen miniprep kit
Plasmid355355pSB1C3-2Zv2Npu:Xyla_circ_noHisH2O50.029650.0725S355
1566
O356O35610-042:00MaxWGrowth1106C1
Qiagen miniprep kit
Plasmid356356pSB1C3-1GateNpuDnaE(N)H2O50.034650.0726S356
1567
O357O35710-042:00MaxWGrowth1107C1
Qiagen miniprep kit
Plasmid357357pSB1C3-1GateNpuDnaE(C)H2O50.028550.0727S357
1568
O358O35810-042:00MaxWGrowth1108C1
Qiagen miniprep kit
Plasmid358358pSB1C3-2Zv2Npu:Xyla(H6)destrCextH2O50.027650.0728S358
1569
O359O35910-042:00MaxWGrowth1109C1
Qiagen miniprep kit
Plasmid359359pSB1C3-T7RBS-FLAG-Smt3-2Zv2NpuH2O50.037150.0729S359
1570
O360O36010-042:00MaxWGrowth1110C2
Qiagen miniprep kit
Plasmid360360pSB1C3-XylanaseH2O50.024850.0730S360
1571
O361O36110-042:00MaxWGrowth1111C1
Qiagen miniprep kit
Plasmid361361pSB1C3-LiLaLy(H6)H2O50.027350.0731S361
1572
O362O36210-042:00MaxWGrowth1112C1
Qiagen miniprep kit
Plasmid362362pSB1C3-XylaH6BBH2O50.023550.0732S362
1573
O363O36310-042:00MaxWGrowth1113C1
Qiagen miniprep kit
Plasmid363363pSB1C3-XylaH6extBBH2O50.019350.0733S363
1574
O271-3O27110-0410:00CaroGrowth1036C1
Qiagen miniprep kit
Plasmid271271pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6110-9.1765
was not sequenced, but used for Coex, seems right
A271DG7164
1575
O271-4O27110-0410:00CaroGrowth1036C2
Qiagen miniprep kit
Plasmid271271pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6115-8.7766A271DG7165
1576
O271-5O27110-0410:00CaroGrowth1036C3
Qiagen miniprep kit
Plasmid271271pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H61000.0767A271
1577
O271-6O27110-0410:00CaroGrowth1036C4
Qiagen miniprep kit
Plasmid271271pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H60.0A271
1578
O271-7O27110-0410:00CaroGrowth1036C5
Qiagen miniprep kit
Plasmid271271pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6#DIV/0!A271
1579
O272-4O27210-0410:00CaroGrowth1095C1
Qiagen miniprep kit
Plasmid272272pSBX1K3-H6-DnaXC-mRFPc-H6168-6.0768
was not sequenced, but used for Coex, seems right
A272#3DG7162
1580
O272-5O27210-0410:00CaroGrowth1095C2
Qiagen miniprep kit
Plasmid272272pSBX1K3-H6-DnaXC-mRFPc-H6189-5.3769A272#3DG7163
1581
O272-6O27210-0410:00CaroGrowth1095C3
Qiagen miniprep kit
Plasmid272272pSBX1K3-H6-DnaXC-mRFPc-H6122-8.2770A272#3
1582
O272-7O27210-0410:00CaroGrowth1095C4
Qiagen miniprep kit
Plasmid272272pSBX1K3-H6-DnaXC-mRFPc-H60.0A272#3
1583
O275-4O27510-0410:00CaroGrowth1096C1
Qiagen miniprep kit
Plasmid275275pSBX1K3-SspDnaXc*-sfGFP^C-H6172-5.8771A275#3DG7166
1584
O275-5O27510-0410:00CaroGrowth1096C2
Qiagen miniprep kit
Plasmid275275pSBX1K3-SspDnaXc*-sfGFP^C-H6172-5.8772A275#3DG7167
1585
O275-6O27510-0410:00CaroGrowth1096C3
Qiagen miniprep kit
Plasmid275275pSBX1K3-SspDnaXc*-sfGFP^C-H60.0A275#3
1586
O275-7O27510-0410:00CaroGrowth1096C4
Qiagen miniprep kit
Plasmid275275pSBX1K3-SspDnaXc*-sfGFP^C-H6169-5.9773A275#3
1587
O312-4O31210-0410:00CaroGrowth1125C1
Qiagen miniprep kit
Plasmid312312pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-225235-4.3774G312#2DG7168
1588
O312-5O31210-0410:00CaroGrowth1125C2
Qiagen miniprep kit
Plasmid312312pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-225289-3.5775G312#2
1589
O312-6O31210-0410:00CaroGrowth1125C3
Qiagen miniprep kit
Plasmid312312pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-225272-3.7776G312#2
1590
O312-7O31210-0410:00CaroGrowth1125C4
Qiagen miniprep kit
Plasmid312312pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-2250.0G312#2
1591
O312-8O31210-0410:00CaroGrowth1125C5
Qiagen miniprep kit
Plasmid312312pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-2250.0G312#2
1592
O313-4O31310-0410:00CaroGrowth1126C1
Qiagen miniprep kit
Plasmid313313pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-168291-3.4777G313#2DG7169ok, good
1593
O313-5O31310-0410:00CaroGrowth1126C2
Qiagen miniprep kit
Plasmid313313pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-168281-3.6778G313#2
1594
O313-6O31310-0410:00CaroGrowth1126C3
Qiagen miniprep kit
Plasmid313313pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-168332-3.0779G313#2
1595
O313-7O31310-0410:00CaroGrowth1126C4
Qiagen miniprep kit
Plasmid313313pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-1680.0G313#2
1596
O313-8O31310-0410:00CaroGrowth1126C5
Qiagen miniprep kit
Plasmid313313pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-1680.0G313#2
1597
O314-4O31410-0410:00CaroGrowth1127C1
Qiagen miniprep kit
Plasmid314314pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225305-3.3780G314#2DG7170
1598
O314-5O31410-0410:00CaroGrowth1127C2
Qiagen miniprep kit
Plasmid314314pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225250-4.0781G314#2DG7171
1599
O314-6O31410-0410:00CaroGrowth1127C3
Qiagen miniprep kit
Plasmid314314pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225338-3.0782G314#2
1600
O315-4O31510-0410:00CaroGrowth1128C1
Qiagen miniprep kit
Plasmid315315pSBX4C5-0GateNpuDnaE_N*_mRFP*1-168183-5.5783G315#2DG7172ok, good
1601
O315-5O31510-0410:00CaroGrowth1128C2
Qiagen miniprep kit
Plasmid315315pSBX4C5-0GateNpuDnaE_N*_mRFP*1-168268-3.7784G315#2DG7173ok, but probably mutation in promoter, if possible use O315-4
1602
O315-6O31510-0410:00CaroGrowth1128C3
Qiagen miniprep kit
Plasmid315315pSBX4C5-0GateNpuDnaE_N*_mRFP*1-168200-5.0785G315#2
1603
O315-7O31510-0410:00CaroGrowth1128C4
Qiagen miniprep kit
Plasmid315315pSBX4C5-0GateNpuDnaE_N*_mRFP*1-1680.0G315#2
1604
O315-8O31510-0410:00CaroGrowth1128C5
Qiagen miniprep kit
Plasmid315315pSBX4C5-0GateNpuDnaE_N*_mRFP*1-1680.0G315#2
1605
O371O37110-0415:00LisaGrowth1135C2
Qiagen miniprep kit
Plasmid371371pSBX4C5-1GateSspDnaB(C)257-8.9734O240
1606
O371-2O37110-0415:00LisaGrowth1135C3
Qiagen miniprep kit
Plasmid371371pSBX4C5-1GateSspDnaB(C)99-15.1735O240
1607
O371-3O37110-0415:00LisaGrowth1136C1
Qiagen miniprep kit
Plasmid371371pSBX4C5-1GateSspDnaB(C)184-5.0736O240-3
1608
O371-4O37110-0415:00LisaGrowth1136C2
Qiagen miniprep kit
Plasmid371371pSBX4C5-1GateSspDnaB(C)1430.0737O240-3
1609
O372O37210-0415:00LisaGrowth1137C1
Qiagen miniprep kit
Plasmid372372pSBX4C5-1GateSspDnaB(C*)188-5.3738O241-2
1610
O372-2O37210-0415:00LisaGrowth1137C2
Qiagen miniprep kit
Plasmid372372pSBX4C5-1GateSspDnaB(C*)198-5.1739O241-2
1611
O372-3O37210-0415:00LisaGrowth1138C1
Qiagen miniprep kit
Plasmid372372pSBX4C5-1GateSspDnaB(C*)136-7.4740O241-3
1612
O372-4O37210-0415:00LisaGrowth1138C2
Qiagen miniprep kit
Plasmid372372pSBX4C5-1GateSspDnaB(C*)150-6.7741O241-3
1613
O373O37310-0415:00LisaGrowth1139C2
Qiagen miniprep kit
Plasmid373373pSBX4C5-1GateSspDnaB(N)175-5.7742O242
1614
O373-2O37310-0415:00LisaGrowth1139C3
Qiagen miniprep kit
Plasmid373373pSBX4C5-1GateSspDnaB(N)174-5.8743O242
1615
O373-3O37310-0415:00LisaGrowth1140C1
Qiagen miniprep kit
Plasmid373373pSBX4C5-1GateSspDnaB(N)189-5.3744O242-2
1616
O373-4O37310-0415:00LisaGrowth1140C2
Qiagen miniprep kit
Plasmid373373pSBX4C5-1GateSspDnaB(N)295-3.4745O242-2
1617
O374O37410-0415:00LisaGrowth1141C2
Qiagen miniprep kit
Plasmid374374pSBX4C5-1GateSspDnaB(N*)118-8.5746O243-2
1618
O374-2O37410-0415:00LisaGrowth1141C3
Qiagen miniprep kit
Plasmid374374pSBX4C5-1GateSspDnaB(N*)198-5.1747O243-2
1619
O369O36910-0415:00LisaGrowth1143C1
Qiagen miniprep kit
Plasmid369369pSBX4C5-1GateSspDnaE(N)207-4.8748O325
1620
O369-2O36910-0415:00LisaGrowth1143C2
Qiagen miniprep kit
Plasmid369369pSBX4C5-1GateSspDnaE(N)203-4.9749O325
1621
O369-3O36910-0415:00LisaGrowth1144C1
Qiagen miniprep kit
Plasmid369369pSBX4C5-1GateSspDnaE(N)188-5.3750O325-2
1622
O369-4O36910-0415:00LisaGrowth1144C3
Qiagen miniprep kit
Plasmid369369pSBX4C5-1GateSspDnaE(N)271-3.7751O325-2
1623
O370O37010-0415:00LisaGrowth1145C2
Qiagen miniprep kit
Plasmid370370pSBX4C5-1GateSspDnaE(C)224-4.5752O326
1624
O370-2O37010-0415:00LisaGrowth1145C3
Qiagen miniprep kit
Plasmid370370pSBX4C5-1GateSspDnaE(C)229-4.4753O326
1625
O370-3O37010-0415:00LisaGrowth1146C2
Qiagen miniprep kit
Plasmid370370pSBX4C5-1GateSspDnaE(C)197-5.1754O326-3
1626
O370-4O37010-0415:00LisaGrowth1146C3
Qiagen miniprep kit
Plasmid370370pSBX4C5-1GateSspDnaE(C)302-3.3755O326-3
1627
O375O37510-0421:00MaxWGrowth1116C1
Qiagen miniprep kit
Plasmid375375pSB1C3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6H2O50.034950.0756S375
1628
O376O37610-0421:00MaxWGrowth1117C1
Qiagen miniprep kit
Plasmid376376pSB1C3-1Gate-NpuDnaE(N)-H6H2O50.046137.0757S376
1629
O377O37710-0421:00MaxWGrowth1118C1
Qiagen miniprep kit
Plasmid377377pSB1C3-H6-1GateNpuDnaE(C)H2O50.030650.0758S377
1630
O378O37810-0421:00MaxWGrowth1119C1
Qiagen miniprep kit
Plasmid378378pSB1C3-T7RBS-H6-sfGFP_T65C-H6H2O50.036650.0759S378
1631
O379O37910-0421:00MaxWGrowth1120C1
Qiagen miniprep kit
Plasmid379379pSB1C3-1GateSspDnaX-S11(C)-H6H2O50.038650.0760S379
1632
O380O38010-0421:00MaxWGrowth1121C1
Qiagen miniprep kit
Plasmid380380pSB1C3-1GateSspDnaX-S11N_H6H2O50.033650.0761S380
1633
O381O38110-0421:00MaxWGrowth1122C1
Qiagen miniprep kit
Plasmid381381pSB1C3-1GateSspGyrB-S11N_H6H2O50.033450.0762S381
1634
O382O38210-0421:00MaxWGrowth1123C1
Qiagen miniprep kit
Plasmid382382pSB1C3-1GateTerDnaE3-S11C_H6H2O50.027350.0763S382
1635
O383O38310-0421:00MaxWGrowth1124C1
Qiagen miniprep kit
Plasmid383383pSB1C3-1GateTerDnaE3-S11(N)-H6H2O50.030450.0764S383
1636
R470R47010-0422:00LisaGel434-2Gel extraction
Restriction
470pSB1C3-BBa_J04450 (E/P)H2O30.01520.6
1637
R471R47110-0422:00LisaGel434-3Gel extraction
Restriction
471pSB1C3-BBa_J04450 (E/P)H2O30.01520.6
1638
R472R47210-0422:00LisaGel434-4Gel extraction
Restriction
472pSB1C3-BBa_J04450 (E/P)H2O30.01520.6
1639
R473R47310-0422:00LisaGel434-5Gel extraction
Restriction
473pSB1C3-BBa_J04450 (E/P)H2O30.01521.2
1640
R474R47410-0422:00LisaGel434-6Gel extraction
Restriction
474pSB1C3-BBa_J04450 (E/P)H2O30.01521.0
1641
R475R47510-0422:00LisaGel434-7Gel extraction
Restriction
475pSB1C3-BBa_J04450 (E/P)H2O30.01521.0
1642
R476R47610-0422:00LisaGel434-8Gel extraction
Restriction
476pSB1C3-BBa_J04450 (E/P)H2O30.01523.8
1643
R477R47710-0422:00LisaGel435-2Gel extraction
Restriction
477pSBX4C5-1GateSspDnaB(C*) (E/P)H2O30.01020.0
1644
R478R47810-0422:00LisaGel435-3Gel extraction
Restriction
478pSBX4C5-1GateSspDnaB(C*) (E/P)H2O30.01020.0
1645
R479R47910-0422:00LisaGel435-4Gel extraction
Restriction
479pSBX4C5-1GateSspDnaB(C*) (E/P)H2O30.01020.0
1646
R480R48010-0422:00LisaGel435-5Gel extraction
Restriction
480pSBX4C5-1GateSspDnaB(C*) (E/P)H2O30.01020.0
1647
R481R48110-0422:00LisaGel435-6Gel extraction
Restriction
481pSBX4C5-1GateSspDnaB(N) (E/P)H2O30.01020.6
1648
R482R48210-0422:00LisaGel435-7Gel extraction
Restriction
482pSBX4C5-1GateSspDnaB(N) (E/P)H2O30.01020.6
1649
R483R48310-0422:00LisaGel435-8Gel extraction
Restriction
483pSBX4C5-1GateSspDnaB(N) (E/P)H2O30.01020.6
1650
R484R48410-0422:00LisaGel436-2Gel extraction
Restriction
484pSBX4C5-1GateSspDnaB(N) (E/P)H2O30.01020.6
1651
R485R48510-0422:00LisaGel436-3Gel extraction
Restriction
485pSBX4C5-1GateSspDnaB(N*) (E/P)H2O30.01020.6
1652
R486R48610-0422:00LisaGel436-4Gel extraction
Restriction
486pSBX4C5-1GateSspDnaB(N*) (E/P)H2O30.01020.6
1653
R487R48710-0422:00LisaGel436-5Gel extraction
Restriction
487pSBX4C5-1GateSspDnaE(N) (E/P)H2O30.01020.1
1654
R488R48810-0422:00LisaGel436-6Gel extraction
Restriction
488pSBX4C5-1GateSspDnaE(N) (E/P)H2O30.01020.1
1655
R489R48910-0422:00LisaGel436-7Gel extraction
Restriction
489pSBX4C5-1GateSspDnaE(N) (E/P)H2O30.01020.1
1656
R490R49010-0422:00LisaGel436-8Gel extraction
Restriction
490pSBX4C5-1GateSspDnaE(N) (E/P)H2O30.01020.1
1657
R491R49110-0422:00LisaGel436-9Gel extraction
Restriction
491pSBX4C5-1GateSspDnaE(C) (E/P)H2O30.01020.5
1658
R492R49210-0422:00LisaGel436-10Gel extraction
Restriction
492pSBX4C5-1GateSspDnaE(C) (E/P)H2O30.01020.5
1659
R493R49310-0422:00LisaGel436-11Gel extraction
Restriction
493pSBX4C5-1GateSspDnaE(C) (E/P)H2O30.01020.5
1660
R494R49410-0422:00LisaGel436-12Gel extraction
Restriction
494pSBX4C5-1GateSspDnaE(C) (E/P)H2O30.01020.5
1661
R495R49510-0422:00LisaGel436-13Gel extraction
Restriction
495pSBX4C5-1GateSspDnaB(C) (E/P)H2O30.01020.0
1662
R496R49610-0422:00LisaGel436-14Gel extraction
Restriction
496pSBX4C5-1GateSspDnaB(C) (E/P)H2O30.01020.0
1663
R424R42410-0422:00CaroRestriction424Gel extraction
Restriction
424pSBX4C5-0GateSspDnaX-S11C_mRFP169-225 (E/X)18-4.0
1664
R425R42510-0422:00CaroRestriction425Gel extraction
Restriction
425pSBX4C5-0GateSspGyrB-S11C_mRFP169-225 (E/X)30-2.1
1665
R426R42610-0422:00CaroRestriction426Gel extraction
Restriction
426pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225 (E/X)16-4.6
1666
R427R42710-0422:00CaroGel437-2Gel extraction
Restriction
427pSBX4C5-0GateSspDnaX-S11N_mRFP1-168 (E/S)8-7.8
1667
R428R42810-0422:00CaroGel437-3Gel extraction
Restriction
428pSBX4C5-0GateSspGyrB-S11N_mRFP1-168 (E/S)7-8.7
1668
R429R42910-0422:00CaroGel437-4Gel extraction
Restriction
429pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168 (E/S)8-8.1
1669
R430R43010-0422:00CaroRestriction430
PCR purification kit
Restriction
430pSBX4C5-0GateSspDnaX-S11C*_mRFP*169-225 (E/X)17-3.1
1670
R431R43110-0422:00CaroRestriction431
PCR purification kit
Restriction
431pSBX4C5-0GateSspGyrB-S11C*_mRFP*169-225 (E/X)21-4.8
1671
R432R43210-0422:00CaroRestriction432
PCR purification kit
Restriction
432pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-225 (E/X)20-2.4
1672
R433R43310-0422:00CaroRestriction433
PCR purification kit
Restriction
433pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-225 (E/X)30-1.7
1673
R434R43410-0422:00CaroRestriction434
PCR purification kit
Restriction
434pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-225 (E/X)28-2.6
1674
R435R43510-0422:00CaroRestriction435
PCR purification kit
Restriction
435pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225 (E/X)23-3.3
1675
R436R43610-0422:00CaroRestriction436
PCR purification kit
Restriction
436pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225 (E/X)26-3.0
1676
R437R43710-0422:00CaroRestriction437
PCR purification kit
Restriction
437pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225 (E/X)30-2.7
1677
R438R43810-0422:00CaroGel437-5Gel extraction
Restriction
438pSBX4C5-0GateSspDnaX-S11N*_mRFP*1-168 (E/S)6-7.7
1678
R439R43910-0422:00CaroGel437-6Gel extraction
Restriction
439pSBX4C5-0GateSspGyrB-S11N*_mRFP*1-168 (E/S)5-18.7
1679
R440R44010-0422:00CaroGel437-7Gel extraction
Restriction
440pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-168 (E/S)5-8.4
1680
R441R44110-0422:00CaroGel437-8Gel extraction
Restriction
441pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-168 (E/S)5-9.0
1681
R442R44210-0422:00CaroGel438-2Gel extraction
Restriction
442pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-168 (E/S)8-8.1
1682
R443R44310-0422:00CaroGel438-3Gel extraction
Restriction
443pSBX4C5-0GateNpuDnaE_N*_mRFP*1-168 (E/S)8-7.4
1683
R444R44410-0422:00CaroGel438-4Gel extraction
Restriction
444pSBX4C5-0GateNpuDnaE_N*_mRFP*1-168 (E/S)8-7.7
1684
R445R44510-0422:00CaroGel438-5Gel extraction
Restriction
445pSBX4C5-0GateNpuDnaE_N*_mRFP*1-168 (E/S)8-8.0
1685
R446R44610-0422:00CaroGel438-6Gel extraction
Restriction
446pSBX1K3-H6-mRFPn-DnaXN-H6 (E/S)6-28.4
1686
R447R44710-0422:00CaroGel438-7Gel extraction
Restriction
447pSBX1K3-H6-mRFPn-DnaXN-H6 (E/S)6-42.4
1687
R448R44810-0422:00CaroGel438-8Gel extraction
Restriction
448pSBX1K3-H6-mRFPn-DnaXN-H6 (E/S)6-13.6
1688
R449R44910-0422:00CaroGel439-2Gel extraction
Restriction
449pSBX1K3-H6-mRFPn-DnaXN*-H6 (E/S)4-7.7
1689
R450R45010-0422:00CaroRestriction450
PCR purification kit
Restriction
450pSBX1K3-H6-DnaXC-mRFPc-H6 (E/X)15-3.2
1690
R451R45110-0422:00CaroRestriction451
PCR purification kit
Restriction
451pSBX1K3-H6-DnaXC-mRFPc-H6 (E/X)15-3.2
1691
R452R45210-0422:00CaroRestriction452
PCR purification kit
Restriction
452pSBX1K3-H6-DnaXC-mRFPc-H6 (E/X)13-3.5
1692
R453R45310-0422:00CaroRestriction453
PCR purification kit
Restriction
453pSBX1K3-H6-DnaXC*-mRFPc-H6 (E/X)5-5.1
1693
R454R45410-0422:00CaroRestriction454
PCR purification kit
Restriction
454pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6 (E/X)13-3.3
1694
R455R45510-0422:00CaroRestriction455
PCR purification kit
Restriction
455pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6 (E/X)17-6.0
1695
R456R45610-0422:00CaroRestriction456
PCR purification kit
Restriction
456pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 (E/X)15-3.0
1696
R457R45710-0422:00CaroRestriction457
PCR purification kit
Restriction
457pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 (E/X)10-4.0
1697
R458R45810-0422:00CaroRestriction458
PCR purification kit
Restriction
458pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 (E/X)10-4.0
1698
R459R45910-0422:00CaroGel439-3Gel extraction
Restriction
459pSBX1K3-DnaXN-sfGFPn-H6 (E/S)8-23.3
1699
R460R46010-0422:00CaroGel439-4Gel extraction
Restriction
460pSBX1K3-DnaXN-sfGFPn-H6 (E/S)8-6.8
1700
R461R46110-0422:00CaroGel439-5Gel extraction
Restriction
461pSBX1K3-DnaXN-sfGFPn-H6 (E/S)8-7.2
1701
R462R46210-0422:00CaroGel439-6Gel extraction
Restriction
462pSBX1K3-DnaXN*-sfGFPn-H6 (E/S)8-20.8
1702
R463R46310-0422:00CaroRestriction463
PCR purification kit
Restriction
463pSBX1K3-SspDnaXc-sfGFP^C-H6 (E/X)22-3.5
1703
R464R46410-0422:00CaroRestriction464
PCR purification kit
Restriction
464pSBX1K3-SspDnaXc*-sfGFP^C-H6 (E/X)19-3.9
1704
R465R46510-0422:00CaroRestriction465
PCR purification kit
Restriction
465pSBX1K3-SspDnaXc*-sfGFP^C-H6 (E/X)19-3.9
1705
R466R46610-0422:00CaroRestriction466
PCR purification kit
Restriction
466pSBX1K3-SspDnaXc*-sfGFP^C-H6 (E/X)20-3.7
1706
R467R46710-0422:00CaroRestriction467
PCR purification kit
Restriction
467pSBX1K3-LOV-BsaIIasB-sfGFPc (E/X)28-3.3
1707
R468R46810-0422:00CaroRestriction468
PCR purification kit
Restriction
468pSBX1K3-LOV-12-DnaXC-sfGFPc (E/X)21-4.0
1708
R469R46910-0422:00CaroRestriction469
PCR purification kit
Restriction
469pSBX1K3-LOV-6-DnaXC-sfGFPc (E/X)25-7.8
1709
R497R49710-0517:30MaxWGel440-2Gel extraction
Restriction
497pSBX4C5-1GateGp41-1(N)-H6 (E/P)H2O20.01412.7
1710
R498R49810-0517:30MaxWGel440-3Gel extraction
Restriction
498pSBX4C5-1GateGp41-1(N)-H6 (E/P)H2O20.01112.4
1711
R499R49910-0517:30MaxWGel440-4Gel extraction
Restriction
499pSBX4C5-H6-1GateGp41-1(C) (E/P)H2O20.068.5
1712
R500R50010-0517:30MaxWGel440-5Gel extraction
Restriction
500pSBX4C5-H6-1GateGp41-1(C) (E/P)H2O20.068.5
1713
R501R50110-0517:30MaxWGel440-6Gel extraction
Restriction
501pSBX4C5-DoubleGateGp41-1(N) (E/P)H2O20.098.2
1714
R502R50210-0517:30MaxWGel440-7Gel extraction
Restriction
502pSBX4C5-DoubleGateGp41-1(N) (E/P)H2O20.078.1
1715
R503R50310-0517:30MaxWGel440-8Gel extraction
Restriction
503pSBX4C5-DoubleGateGp41-1(C) (E/P)H2O20.01112.1
1716
R504R50410-0517:30MaxWGel440-9Gel extraction
Restriction
504pSBX4C5-DoubleGateGp41-1(C) (E/P)H2O20.01112.1
1717
R505R50510-0517:30MaxWGel440-10Gel extraction
Restriction
505pSBX4C5-DoubleNpuDnaE(N) (E/P)H2O20.098.4
1718
R506R50610-0517:30MaxWGel440-11Gel extraction
Restriction
506pSBX4C5-DoubleNpuDnaE(N) (E/P)H2O20.078.2
1719
R507R50710-0517:30MaxWGel440-12Gel extraction
Restriction
507pSBX4C5-DoubleGateNpuDnaE(C) (E/P)H2O20.01312.4
1720
R509R50910-0517:30MaxWGel441-2, 441-3Gel extraction
Restriction
509pSB1C3-1Gate-NpuDnaE(N)-H6 (E/P)H2O20.03215.0
1721
R511R51110-0517:30MaxWGel441-4, 441-5Gel extraction
Restriction
511pSB1C3-1Gate-NpuDnaE(N)-H6 (E/P)H2O20.0289.2
1722
R513R51310-0517:30MaxWGel441-6, 441-7Gel extraction
Restriction
513pSB1C3-1Gate-NpuDnaE(N)-H6 (E/P)H2O20.02416.8
1723
R515R51510-0517:30MaxWGel441-8, 441-9Gel extraction
Restriction
515pSB4C5-BBa_J04450 (E/P)H2O20.0169.7
1724
R517R51710-0517:30MaxWGel441-10, 441-11Gel extraction
Restriction
517pSB4C5-BBa_J04450 (E/P)H2O20.0169.3
1725
R519R51910-0517:30MaxWGel
441-12, 441-13, 442-2
Gel extraction
Restriction
519pSB4C5-BBa_J04450 (E/P)H2O20.0197.7
1726
R522R52210-0517:30MaxWGel442-3Gel extraction
Restriction
522pSBX1A3-BBa_I13507 (E/P)H2O20.01416.3
1727
R523R52310-0517:30MaxWGel442-4, 442-5Gel extraction
Restriction
523pSBX1C3-BBa_I13507 (E/P)H2O20.0712.5
1728
R524R52410-0517:30MaxWGel442-6Gel extraction
Restriction
524pSBX1K3-BBa_I13507 (E/P)H2O20.02717.9
1729
R525R52510-0517:30MaxWGel442-7Gel extraction
Restriction
525pSBX1T3-T7RBS-IGEMHD_v3 (E/P)H2O20.01616.3
1730
R526R52610-0517:30MaxWGel442-8, 442-9Gel extraction
Restriction
526pSBX4A5-BBa_I13507 (E/P)H2O20.01615.8
1731
R527R52710-0517:30MaxWGel442-10Gel extraction
Restriction
527pSBX4C5-BBa_I13507 (E/P)H2O20.01415.0
1732
R528R52810-0517:30MaxWGel442-11Gel extraction
Restriction
528pSBX4K5-BBa_I13507 (E/P)H2O20.039.0
1733
O398O39810-0519:54JanGrowth1149C1
Qiagen miniprep kit
Plasmid398398pSB1C3-1GateNpuDnaE(N)*-H6EB50.032650.0786S398
1734
O399O39910-0519:54JanGrowth1150C1
Qiagen miniprep kit
Plasmid399399pSB1C3-1GateSspGyrB-S11C_H6EB50.038850.0787S399
1735
O400O40010-0519:54JanGrowth1151C1
Qiagen miniprep kit
Plasmid400400pSB1C3-H6-1GateNpu*(C)_v2EB50.026450.0788S400
1736
O401O40110-0519:54JanGrowth1152C1
Qiagen miniprep kit
Plasmid401401pSB1C3-1Gate-NpuDnaE(N)-H6-H6-1GateNpuDnaE(C)EB50.0050.0789
accidentally used QIAshredder column
S401
1737
O402O40210-0519:54JanGrowth1153C1
Qiagen miniprep kit
Plasmid402402pSB1C3-1GateNpuDnaE(N)*-H6-H6-1GateNpu*(C)_v2EB50.0050.0790
accidentally used QIAshredder column
S402
1738
O403O40310-0519:54JanGrowth1154C1
Qiagen miniprep kit
Plasmid403403pSB1C3-T7RBS_BBv2EB50.0050.0791
accidentally used QIAshredder column
S403
1739
O403-2O40310-0519:54JanGrowth1154C2
Qiagen miniprep kit
Plasmid403403pSB1C3-T7RBS_BBv2EB50.0050.0792
accidentally used QIAshredder column
S403
1740
O284-3O28410-0519:54JanGrowth1155C1
Qiagen miniprep kit
Plasmid284284pSBX1K3-2Zv2Npu:LaLys+bad1EB50.0050.0793
accidentally used QIAshredder column
S284
1741
O63-7O6310-0523:00AnnaGrowth240C2-2
Qiagen miniprep kit
Plasmid6363pSBX4K5-BBa_I13507H2O30.011921.6L63#3
1742
R530R53010-0611:10SilvanGel443-2Gel extraction
Restriction
530pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP) (E/P)EB25.0313.3
1743
R531R53110-0611:10SilvanGel443-3Gel extraction
Restriction
531pSBX1K3-T7RBS-H6-Smt3-0ZSortA (E/P)EB25.088.9
1744
R532R53210-0611:10SilvanGel443-4Gel extraction
Restriction
532pSBX1K3-T7RBS-H6-0ZSortA (E/P)EB25.089.0
1745
P234-4P23410-0611:10SilvanGel444-2,444-3Gel extractionPP234pSB1C3_SortAc-H6_TEVc-BsaIEB25.02125.0
1746
P521P52110-0611:10SilvanGel444-4,444-5Gel extractionPP521pSB1C3_TAATAA_Smt3EB25.08225.0
1747
P522P52210-0611:10SilvanGel444-6,444-7Gel extractionPP522H6Smt3_BBprefix_DNMT1/BB2EB25.07025.0
1748
P235-3P23510-0611:10SilvanGel444-10,444-11Gel extractionPP235mRFPexp_TEVc-BsaI_SortAc-H6-BBsuffixEB25.013725.0
1749
P523P52310-0611:10SilvanGel444-12,444-13Gel extractionPP523DNMT1/BB2_DNMT1/BB1_DNMT1/BB3EB25.02925.0
1750
P524P52410-0611:10SilvanGel444-14,444-15Gel extractionPP524DNMT1/BB3_DNMT1/BB2_RFC10suffixEB25.03425.0
1751
R529R52910-0613:00AnnaGel445-2,445-3Gel extraction
Restriction
529pSBX4K5-BBa_I13507 (E/P)H2O20.01013.4
1752
O414O41410-0616:50LisaGrowth1156C2
Qiagen miniprep kit
Plasmid414414pSB1C3-1GateSspDnaB(C*)H2O50.024145.0811S414DG7142, IC5216, IC5221bad, SspDnaEN, SspDnaEN
1753
O414-2O41410-0616:50LisaGrowth1157C1
Qiagen miniprep kit
Plasmid414414pSB1C3-1GateSspDnaB(C*)H2O50.022645.0812S414#2DG7143
1754
O414-3O41410-0616:50LisaGrowth1158C1
Qiagen miniprep kit
Plasmid414414pSB1C3-1GateSspDnaB(C*)H2O50.016145.0813S414#3DG7144
1755
O414-4O41410-0616:50LisaGrowth1159C2
Qiagen miniprep kit
Plasmid414414pSB1C3-1GateSspDnaB(C*)H2O50.019345.0814S414#4DG7145
1756
O415O41510-0616:50LisaGrowth1160C2
Qiagen miniprep kit
Plasmid415415pSB1C3-1GateSspDnaB(N)H2O50.014445.0815S415DG7146, IC5213, IC5218bad, actually SspDnaEC, actually SspDnaEC
1757
O415-2O41510-0616:50LisaGrowth1161C1
Qiagen miniprep kit
Plasmid415415pSB1C3-1GateSspDnaB(N)H2O50.011145.0816S415#2DG7147bad
1758
O415-3O41510-0616:50LisaGrowth1162C1
Qiagen miniprep kit
Plasmid415415pSB1C3-1GateSspDnaB(N)H2O50.031545.0817S415#3DG7148bad
1759
O415-4O41510-0616:50LisaGrowth1163C2
Qiagen miniprep kit
Plasmid415415pSB1C3-1GateSspDnaB(N)H2O50.021345.0818S415#4DG7149bad
1760
O416O41610-0616:50LisaGrowth1164C1
Qiagen miniprep kit
Plasmid416416pSB1C3-1GateSspDnaB(N*)H2O50.018545.0819S416DG7150, IC5215, IC5220bad, actually SspDnaBC!!!!!! wuhuuuuuuuuuu yippie, actually SspDnaBC!!!!!! wuhuuuuuuuuuu yippie
1761
O416-2O41610-0616:50LisaGrowth1165C2
Qiagen miniprep kit
Plasmid416416pSB1C3-1GateSspDnaB(N*)H2O50.07345.0824S416#2DG7151not mixed up, missing?
1762
O411O41110-0616:50LisaGrowth1166C2
Qiagen miniprep kit
Plasmid411411pSB1C3-1GateSspDnaE(N)H2O50.014445.0820S411DG7152
1763
O411-2O41110-0616:50LisaGrowth1167C1
Qiagen miniprep kit
Plasmid411411pSB1C3-1GateSspDnaE(N)H2O50.018545.0821S411#2DG7153
1764
O411-3O41110-0616:50LisaGrowth1168C1
Qiagen miniprep kit
Plasmid411411pSB1C3-1GateSspDnaE(N)H2O50.022645.0822S411#3DG7154
1765
O411-4O41110-0616:50LisaGrowth1169C1
Qiagen miniprep kit
Plasmid411411pSB1C3-1GateSspDnaE(N)H2O50.016645.0823S411#4DG7155, IC5217, IC5222, actually SspDnaBC*, actually SspDnaBC*
1766
O412O41210-0616:50LisaGrowth1170C2
Qiagen miniprep kit
Plasmid412412pSB1C3-1GateSspDnaE(C)H2O50.011345.0825bad parentS412DG7156
1767
O412-2O41210-0616:50LisaGrowth1171C1
Qiagen miniprep kit
Plasmid412412pSB1C3-1GateSspDnaE(C)H2O50.033745.0826bad parentS412#2DG7157, IC5260, actually sspdnaBN
1768
O412-3O41210-0616:50LisaGrowth1172C1
Qiagen miniprep kit
Plasmid412412pSB1C3-1GateSspDnaE(C)H2O50.08045.0827bad parentS412#3DG7158, IC5261, actually sspdnaBN
1769
O412-4O41210-0616:50LisaGrowth1173C2
Qiagen miniprep kit
Plasmid412412pSB1C3-1GateSspDnaE(C)H2O50.013945.0828bad parentS412#4DG7159
1770
O413O41310-0616:50LisaGrowth1175C1
Qiagen miniprep kit
Plasmid413413pSB1C3-1GateSspDnaB(C)H2O50.09045.0829S413#2DG7160
1771
O413-2O41310-0616:50LisaGrowth1175C2
Qiagen miniprep kit
Plasmid413413pSB1C3-1GateSspDnaB(C)H2O50.05845.0830S413#2DG7161, IC5214, IC5219bad, actually SspDnaBN*, actually SspDnaBN*
1772
O401-2O40110-071:20MaxWGrowth1152C1-1
Qiagen miniprep kit
Plasmid401401pSB1C3-1Gate-NpuDnaE(N)-H6-H6-1GateNpuDnaE(C)H2O50.052750.0S401
1773
O402-2O40210-071:20MaxWGrowth1153C1-1
Qiagen miniprep kit
Plasmid402402pSB1C3-1GateNpuDnaE(N)*-H6-H6-1GateNpu*(C)_v2H2O50.051150.0S402
1774
O403-3O40310-071:20MaxWGrowth1154C1-1
Qiagen miniprep kit
Plasmid403403pSB1C3-T7RBS_BBv2H2O50.034550.0S403IC5223is actually T7prom
1775
O403-4O40310-071:20MaxWGrowth1154C2-1
Qiagen miniprep kit
Plasmid403403pSB1C3-T7RBS_BBv2H2O50.028550.0S403IC5224is actually T7prom
1776
O284-4O28410-071:20MaxWGrowth1155C1-1
Qiagen miniprep kit
Plasmid284284pSBX1K3-2Zv2Npu:LaLys+bad1H2O50.037350.0S284
1777
O417O41710-071:20MaxWGrowth1179C1
Qiagen miniprep kit
Plasmid417417pSB1C3-1GateGp41-1(N)-H6H2O50.034250.0794S417
1778
O418O41810-071:20MaxWGrowth1181C1
Qiagen miniprep kit
Plasmid418418pSB1C3-H6-1GateGp41-1(C)H2O50.032450.0795S418
1779
O419O41910-071:20MaxWGrowth1183C1
Qiagen miniprep kit
Plasmid419419pSB1C3-DoubleGateGp41-1(N)H2O50.041650.0796S419
1780
O420O42010-071:20MaxWGrowth1185C1
Qiagen miniprep kit
Plasmid420420pSB1C3-DoubleGateGp41-1(C)H2O50.040550.0797S420
1781
O421O42110-071:20MaxWGrowth1187C1
Qiagen miniprep kit
Plasmid421421pSB1C3-DoubleNpuDnaE(N)H2O50.049250.0798S421
1782
O422O42210-071:20MaxWGrowth1189C1
Qiagen miniprep kit
Plasmid422422pSB1C3-DoubleGateNpuDnaE(C)H2O50.038650.0799S422
1783
O404O40410-071:20MaxWGrowth1190C1
Qiagen miniprep kit
Plasmid404404pSBX1A3-BBa_J04450H2O50.042850.0800S404
1784
O405O40510-071:20MaxWGrowth1191C1
Qiagen miniprep kit
Plasmid405405pSBX1C3-BBa_J04450H2O50.025050.0801S405
1785
O406O40610-071:20MaxWGrowth1192C1
Qiagen miniprep kit
Plasmid406406pSBX1K3-BBa_J04450H2O50.047050.0802S406
1786
O408O40810-071:20MaxWGrowth1194C1
Qiagen miniprep kit
Plasmid408408pSBX4A5-BBa_J04450H2O50.025850.0803S408
1787
O409O40910-071:20MaxWGrowth1195C1
Qiagen miniprep kit
Plasmid409409pSBX4C5-BBa_J04450H2O50.052450.0804S409
1788
R532-2R532?10-0713:40LisaRestriction532
Restriction
532pSBX1K3-T7RBS-H6-0ZSortA (E/P)0.0
1789
R532-3R532?10-0713:40LisaRestriction532
Restriction
532pSBX1K3-T7RBS-H6-0ZSortA (E/P)0.0
1790
R532-4R532?10-0713:40LisaRestriction532
Restriction
532pSBX1K3-T7RBS-H6-0ZSortA (E/P)0.0
1791
R532-5R532?10-0713:40LisaRestriction532
Restriction
532pSBX1K3-T7RBS-H6-0ZSortA (E/P)0.0
1792
R532-6R532?10-0713:40LisaRestriction532
Restriction
532pSBX1K3-T7RBS-H6-0ZSortA (E/P)0.0
1793
O386O38610-0716:00CaroGrowth1205C1
Qiagen miniprep kit
Plasmid386386pSBX4C5-0GateTer*-coex_mRFP168/1693000.0831S386#3IC5185, IC5186good, ok, good, ok
1794
O387O38710-0716:00CaroGrowth1207C1
Qiagen miniprep kit
Plasmid387387pSBX4C5-0GateNpu*-coex_mRFP168/1693200.0832S387#2IC5187, IC5188good, ok, good, ok
1795
O387-2O38710-0716:00CaroGrowth1208C1
Qiagen miniprep kit
Plasmid387387pSBX4C5-0GateNpu*-coex_mRFP168/1690.0833S387#3
1796
O384O38410-0716:00CaroGrowth1201C2
Qiagen miniprep kit
Plasmid384384pSBX4C5-0GateDnaX*-coex_mRFP168/1695500.0834S384IC5189, IC5190no DnaXN, no DNnaXN
1797
O386-2O38610-0716:00CaroGrowth1203C1
Qiagen miniprep kit
Plasmid386386pSBX4C5-0GateTer*-coex_mRFP168/1690.0835S386
1798
O386-3O38610-0716:00CaroGrowth1204C2
Qiagen miniprep kit
Plasmid386386pSBX4C5-0GateTer*-coex_mRFP168/1690.0836S386#2
1799
O397O39710-0716:00CaroGrowth1209C1
Qiagen miniprep kit
Plasmid397397pSBX1K3-DnaX-mRFP168/169_coex2300.0837S397IC5191, IC5192good, ok, good, ok
1800
O255-3O25510-0716:00CaroGrowth1198C1
Qiagen miniprep kit
Plasmid255255pSBX4C5-0GateSspDnaX-coex_mRFP168/1693400.0838S255#3IC5193, IC5194good, ok, good, ok
1801
O255-4O25510-0716:00CaroGrowth1198C3
Qiagen miniprep kit
Plasmid255255pSBX4C5-0GateSspDnaX-coex_mRFP168/1690.0839S255#3
1802
O256O25610-0716:00CaroGrowth1199C1
Qiagen miniprep kit
Plasmid256256pSBX4C5-0GateSspGyrB-coex_mRFP168/1693600.0840S256#2IC5195, IC5196good, ok, good, ok
1803
O257-3O25710-0716:00CaroGrowth1200C1
Qiagen miniprep kit
Plasmid257257pSBX4C5-0GateTerDnaE3-coex_mRFP168/1695500.0841S257#3IC5197, IC5198mutations after Biobrick, good ok, mutations after Biobrick, good ok
1804
O393O39310-0716:00CaroGrowth1213C2
Qiagen miniprep kit
Plasmid393393pSBX1K3-H6-LOV-BsaIIasB-mRFP_coex2600.0842S393IC5199, IC5200ok, good, ok, good
1805
O396O39610-0716:00CaroGrowth1212C1
Qiagen miniprep kit
Plasmid396396pSBX1K3-DnaX-mRFP*168/169_coex2500.0843S396IC5201, IC5202ok, good, ok, good
1806
O396-2O39610-0716:00CaroGrowth1212C2
Qiagen miniprep kit
Plasmid396396pSBX1K3-DnaX-mRFP*168/169_coex0.0844S396
1807
O397-2O39710-0716:00CaroGrowth1211C1
Qiagen miniprep kit
Plasmid397397pSBX1K3-DnaX-mRFP168/169_coex0.0845S397#3
1808
O397-3O39710-0716:00CaroGrowth1210C2
Qiagen miniprep kit
Plasmid397397pSBX1K3-DnaX-mRFP168/169_coex0.0846S397#2
1809
O395O39510-0716:00CaroGrowth1215C1
Qiagen miniprep kit
Plasmid395395pSBX1K3-H6-LOV-6-DnaXC-mRFP_coex3000.0847S395IC5203, IC5204ok, good, probably frameshift after CDS, ok, good, probably frameshift after CDS
1810
O395-2O39510-0716:00CaroGrowth1216C1
Qiagen miniprep kit
Plasmid395395pSBX1K3-H6-LOV-6-DnaXC-mRFP_coex0.0848S395#2
1811
O395-3O39510-0716:00CaroGrowth1217C1
Qiagen miniprep kit
Plasmid395395pSBX1K3-H6-LOV-6-DnaXC-mRFP_coex0.0849S395#3
1812
O391O39110-0716:00CaroGrowth1218C1
Qiagen miniprep kit
Plasmid391391pSBX1K3-DnaX-sfGFP_coex3300.0850S391IC5205, IC5206ok, good, ok,good
1813
O392O39210-0716:00CaroGrowth1219C1
Qiagen miniprep kit
Plasmid392392pSBX1K3-DnaX*-sfGFP_coex2100.0851S392IC5207, IC5208ok, good, ok,good
1814
O392-2O39210-0716:00CaroGrowth1220C3
Qiagen miniprep kit
Plasmid392392pSBX1K3-DnaX*-sfGFP_coex0.0852S392#2
1815
O392-3O39210-0716:00CaroGrowth1221C1
Qiagen miniprep kit
Plasmid392392pSBX1K3-DnaX*-sfGFP_coex0.0853S392#3
1816
O388O38810-0716:00CaroGrowth1222C1
Qiagen miniprep kit
Plasmid388388pSBX1K3-LOV-BsaIIasB-sfGFP_coex1900.0854S388IC5209, IC5210ok, good, ok,good
1817
O389O38910-0716:00CaroGrowth1223C1
Qiagen miniprep kit
Plasmid389389pSBX1K3-LOV-12-DnaXC-sfGFP_coex2700.0855S389IC5211, IC5212ok, good, ok,good
1818
O235O23510-0718:30SilvanGrowth1226C2
Qiagen miniprep kit
Plasmid235235pSB1C3-Smt3-2ZSortA-H6 (mRFP)1660.0858G235IC5225, IC5231C->A point mutation in mRFP barcode, mixture of mRFP and ccdB
1819
O235-2O23510-0718:30SilvanGrowth1226C3
Qiagen miniprep kit
Plasmid235235pSB1C3-Smt3-2ZSortA-H6 (mRFP)1980.0859G235IC5226, C->A point mutation in mRFP barcode,
1820
O234O23410-0718:30SilvanGrowth1228C2
Qiagen miniprep kit
Plasmid234234pSB1C3-T7RBS-Smt3-2ZSortA-H6 (mRFP)2270.0861S234
1821
O234-2O23410-0718:30SilvanGrowth1228C3
Qiagen miniprep kit
Plasmid234234pSB1C3-T7RBS-Smt3-2ZSortA-H6 (mRFP)2770.0862S234
1822
O423O42310-0718:30SilvanGrowth1229C1
Qiagen miniprep kit
Plasmid423423pSB1C3-T7RBS-H6-Smt3-0ZSortA2060.0863S423
1823
O423-2O42310-0718:30SilvanGrowth1229C2
Qiagen miniprep kit
Plasmid423423pSB1C3-T7RBS-H6-Smt3-0ZSortA2640.0864S423
1824
O423-3O42310-0718:30SilvanGrowth1229C3
Qiagen miniprep kit
Plasmid423423pSB1C3-T7RBS-H6-Smt3-0ZSortA2970.0865S423
1825
O425O42510-0718:30SilvanGrowth1230C1
Qiagen miniprep kit
Plasmid425425pSB1C3-T7RBS-H6-0ZSortA2420.0866S425
1826
O425-2O42510-0718:30SilvanGrowth1230C2
Qiagen miniprep kit
Plasmid425425pSB1C3-T7RBS-H6-0ZSortA2110.0867S425
1827
O425-3O42510-0718:30SilvanGrowth1230C3
Qiagen miniprep kit
Plasmid425425pSB1C3-T7RBS-H6-0ZSortA3250.0868S425
1828
O177-4O17710-0718:30SilvanGrowth1231C1
Qiagen miniprep kit
Plasmid177177pSB1C3-2ZSortA-H62870.0869A177#4IC5227, IC5232ok, ok
1829
O177-5O17710-0718:30SilvanGrowth1231C2
Qiagen miniprep kit
Plasmid177177pSB1C3-2ZSortA-H62990.0870A177#4IC5228, IC5233ok, ok
1830
O177-6O17710-0718:30SilvanGrowth1231C3
Qiagen miniprep kit
Plasmid177177pSB1C3-2ZSortA-H65050.0871A177#4
1831
O426O42610-0718:30SilvanGrowth1232C1
Qiagen miniprep kit
Plasmid426426pSB1C3-H6-Smt3-mDNMT1(731-1602)2790.0872A426
IC5229, IC5234, IC5236, DD1482
is actually pSB1C3-2ZSortA-H6 with silent G->T mutation in mRFP, is actually pSB1C3-2ZSortA-H6 with silent G->T mutation in mRFP, no result, is actually pSB1C3-2ZSortA-H6 with silent G->T mutation in mRFP
1832
O426-2O42610-0718:30SilvanGrowth1232C2
Qiagen miniprep kit
Plasmid426426pSB1C3-H6-Smt3-mDNMT1(731-1602)3230.0873A426
IC5230, IC5235, DD1481, DD1483
ok, ok, ok, ok
1833
O426-3O42610-0718:30SilvanGrowth1232C3
Qiagen miniprep kit
Plasmid426426pSB1C3-H6-Smt3-mDNMT1(731-1602)4110.0874A426
1834
R561R56110-0723:40CaroRestriction561
PCR purification kit
Restriction
561pSBX1K3-LOV-12-DnaXC-sfGFPc (E/X)21-3.1
1835
R562R56210-0723:40CaroRestriction562
PCR purification kit
Restriction
562pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6 (E/X)180.0
1836
R563R56310-0723:40CaroRestriction563
PCR purification kit
Restriction
563pSBX4C5-0GateSspGyrB-S11C*_mRFP*169-225 (E/X)3-7.9
1837
R564R56410-0723:40CaroGel458-2Gel extraction
Restriction
564pSBX4C5-0GateSspGyrB-S11N*_mRFP*1-168 (E/S)4-4.8
1838
O394O39410-0818:00CaroGrowth1214C5
Qiagen miniprep kit
Plasmid394394pSBX1K3-H6-LOV-12-DnaXC-mRFP_coex0.0S394DD2689, DD2688ok, good, ok, good
1839
O260-6O26010-1017:00CaroGrowth1245C1
Qiagen miniprep kit
Plasmid260260pSBX1K3-BBa_I13507-GSGIDECHT1210.0885O260-5DD2690, DD2691,
1840
O260-7O26010-1017:00CaroGrowth1245C2
Qiagen miniprep kit
Plasmid260260pSBX1K3-BBa_I13507-GSGIDECHT1450.0886O260-5DD2692, DD2693,
1841
O261-4O26110-1017:00CaroGrowth1246C1
Qiagen miniprep kit
Plasmid261261pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha1370.0890O261-3DD2694, DD2695,
1842
O261-5O26110-1017:00CaroGrowth1246C2
Qiagen miniprep kit
Plasmid261261pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha1070.0891O261-3DD2696, DD2697,
1843
O261-6O26110-1017:00CaroGrowth1256C1
Qiagen miniprep kit
Plasmid261261pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha1070.0892O261-3DD2698, DD2699,
1844
R85-2R8510-0821:00MaxWOligos41,42Annealing
Restriction
85T7RBS_BioBrick_v2H2O500.03499.1
1845
R179R17908-10AnnaOligos229,232Annealing
Restriction
179Dnmt1_Substrate_fullymethylated0.0
1846
R180R18008-10AnnaOligos230,231Annealing
Restriction
180Dnmt1_Substrate_unmethylated0.0
1847
R181R18108-10AnnaOligos227,228Annealing
Restriction
181Dnmt1_Substrate_1stMedCsemimethylated0.0
1848
R182R18208-10AnnaOligos227,230Annealing
Restriction
182Dnmt1_Substrate_1stMedChemimethylated0.0
1849
N1N1SilvanLysis1ManualHisProtein1?
50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0
0.0
1850
N2N2SilvanLysis3ManualHisProtein2?
50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0
0.0
1851
N3N3SilvanLysis4ManualHisProtein3?
50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0
0.0
1852
N4N4SilvanLysis5ManualHisProtein4?
50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0
0.0
1853
N5N5SilvanLysis6ManualHisProtein5?
50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0
0.0
1854
N6N6SilvanLysis7ProfiniaProtein6?
50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0
0.0
1855
N7N7SilvanLysis8ManualHisProtein7?
50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0
0.0
1856
N8N8SilvanLysis9ManualHisProtein8?
50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0
0.0
1857
N9N9SilvanLysis10ManualHisProtein9?
10 mM Tris, 100 mM potassium phosphate, 200 mM NaCl, 300 mM imidazole, pH 8.0
0.016
1858
N10N10
Constantin, Silvan
Lysis46ProfiniaProtein10?
50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0
0.0
1859
N11N11
Constantin, Silvan
Lysis47ProfiniaProtein11?
50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0
0.0
1860
N1-2N1SilvanPurificationN1/7DialysisProtein1?
20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5
0.0
1861
N2-2N2SilvanPurificationN2/10DialysisProtein2?
20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5
0.0
1862
N2-3N2SilvanPurificationN2/10DialysisProtein2?
20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5
0.0
10 Units SUMO Protease were added to the affinity-purified protein before Dialysis
1863
N3-2N3SilvanPurificationN3/10DialysisProtein3?
20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5
0.0
1864
N4-2N4SilvanPurificationN4/4DialysisProtein4?
20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5
0.0
1865
N5-2N5SilvanPurificationN5/10DialysisProtein5?
20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5
0.0
1866
N6-2N6SilvanPurificationN6/10DialysisProtein6?
20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5
0.0
1867
N7-2N7SilvanPurificationN7/10DialysisProtein7?
20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5
0.0
1868
N8-2N8SilvanPurificationN8/10DialysisProtein8?
20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5
0.0
1869
N9-2N9SilvanPurificationN9/10DiafiltrationProtein9?
20 mM Tris, 150 mM NaCl, pH 8.0
0.0
1870
N10-2N10SilvanPurificationN10/4DiafiltrationProtein10?
50 mM sodium phosphate, 0.675 M NaCl, pH 6.0
0.0
1871
N11-2N11SilvanPurificationN11/4DiafiltrationProtein11?
50 mM sodium phosphate, 0.675 M NaCl, pH 6.0
0.0
1872
N1-3N1SilvanPurificationN1-2ConcentrationProtein1?0.0
1873
N2-4N2SilvanPurificationN2-2ConcentrationProtein2?0.0
1874
N2-5N2SilvanPurificationN2-3ConcentrationProtein2?0.0
1875
N3-3N3SilvanPurificationN3-2ConcentrationProtein3?0.0
1876
N4-3N4SilvanPurificationN4-2ConcentrationProtein4?0.0
1877
N5-3N5SilvanPurificationN5-2ConcentrationProtein5?0.0
1878
N6-3N6SilvanPurificationN6-2ConcentrationProtein6?0.0
1879
N7-3N7SilvanPurificationN7-2ConcentrationProtein7?0.0
1880
N8-3N8SilvanPurificationN8-2ConcentrationProtein8?0.0
1881
N1-4N1SilvanPurificationN1/8DialysisProtein1?
20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5
0.0
1882
0-600?0.0
1883
0-700?0.0
1884
0-800?0.0
1885
0-900?0.0
1886
0-1000?0.0
1887
0-1100?0.0
1888
0-1200?0.0
1889
0-1300?0.0
1890
0-1400?0.0
1891
0-1500?0.0
1892
0-1600?0.0
1893
0-1700?0.0
1894
0-1800?0.0
1895
0-1900?0.0
1896
0-2000?0.0
1897
0-2100?0.0
1898
0-2200?0.0
1899
0-2300?0.0
1900
0-2400?0.0
1901
0-2500?0.0
1902
0-2600?0.0
1903
0-2700?0.0
1904
0-2800?0.0
1905
0-2900?0.0
1906
0-3000?0.0
1907
0-3100?0.0
1908
0-3200?0.0
1909
0-3300?0.0
1910
0-3400?0.0
1911
0-3500?0.0
1912
0-3600?0.0
1913
0-3700?0.0
1914
0-3800?0.0
1915
0-3900?0.0
1916
0-4000?0.0
1917
0-4100?0.0
1918
0-4200?0.0
1919
0-4300?0.0
1920
0-4400?0.0
1921
0-4500?0.0
1922
0-4600?0.0
1923
0-4700?0.0
1924
0-4800?0.0
1925
0-4900?0.0
1926
0-5000?0.0
1927
0-5100?0.0
1928
0-5200?0.0
1929
0-5300?0.0
1930
0-5400?0.0
1931
0-5500?0.0
1932
0-5600?0.0
1933
0-5700?0.0
1934
0-5800?0.0
1935
0-5900?0.0
1936
0-6000?0.0
1937
0-6100?0.0
1938
0-6200?0.0
1939
0-6300?0.0
1940
0-6400?0.0
1941
0-6500?0.0
1942
0-6600?0.0
1943
0-6700?0.0
1944
0-6800?0.0
1945
0-6900?0.0
1946
0-7000?0.0
1947
0-7100?0.0
1948
0-7200?0.0
1949
0-7300?0.0
1950
0-7400?0.0
1951
0-7500?0.0
1952
0-7600?0.0
1953
0-7700?0.0
1954
0-7800?0.0
1955
0-7900?0.0
1956
0-8000?0.0
1957
0-8100?0.0
1958
0-8200?0.0
1959
0-8300?0.0
1960
0-8400?0.0
1961
0-8500?0.0
1962
0-8600?0.0
1963
0-8700?0.0
1964
0-8800?0.0
1965
0-8900?0.0
1966
0-9000?0.0
1967
0-9100?0.0
1968
0-9200?0.0
1969
0-9300?0.0
1970
0-9400?0.0
1971
0-9500?0.0
1972
0-9600?0.0
1973
0-9700?0.0
1974
0-9800?0.0
1975
0-9900?0.0
1976
0-10000?0.0
1977
0-10100?0.0
1978
0-10200?0.0
1979
0-10300?0.0
1980
0-10400?0.0
1981
0-10500?0.0
1982
0-10600?0.0
1983
0-10700?0.0
1984
0-10800?0.0
1985
0-10900?0.0
1986
0-11000?0.0
1987
0-11100?0.0