Team:Goettingen/project overview/project wetlab
From 2014.igem.org
Project
Wet lab
Approach
Our experimental approach can be summarized as follows:
1. Organisms and surface proteins selection
2. Surface protein amplification and cloning
3. Yeast two-hybrid assay with an existing peptide library
4. Peptide functionalization
Results summary
These are the genes and the organisms we selected:
Number | Gene | Organism |
---|---|---|
1 | mp65 | N/A |
2 | tos1 | N/A |
3 | sim1 | N/A |
4 | ssr1 | N/A |
5 | pir4 | N/A |
6 | scw4 | N/A |
7 | pir3 | N/A |
8 | utr2 | N/A |
9 | crf2full | A. fumigatus |
10 | crf2act | A. fumigatus |
11 | bgleno | A. fumigatus |
12 | ecm33 | A. fumigatus |
13 | sun1 | A. fumigatus |
14 | als1 | N/A |
15 | als3 | N/A |
16 | roda | A. fumigatus |
17 | roda | A. nidulans |
18 | xlna | A. nidulans |
19 | npc2 | A. nidulans |
20 | sho1 | A. nidulans |
21 | mid2 | S. cerevisiae |
22 | sed1 | S. cerevisiae |
23 | prm1 b | A. fumigatus |
24 | crf1 | A. fumigatus |
25 | ama1 | T. gondii |
26 | eglc | A. fumigatus |
27 | cwp1 | S. cerevisiae |
28 | cwp2 | S. cerevisiae |
29 | tir4 | S. cerevisiae |
30 | rom5 | T. gondii |
31 | rom4 | T. gondii |
32 | gra7 | T. gondii |
33 | eglc | A. nidulans |
34 | tir1 | S. cerevisiae |
35 | bgleex | A. fumigatus |
The results for all the genes we selected are summarized in this file.