Team:Hong Kong-CUHK/project-5.html

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Control enzymatic reactions

• Voltage switch allows fast and easy control of enzymatic reaction rate by electricity.
• We are working on demonstrating this with laccase and dioxygenase, and also BiFC

Bacterial Screening

• Utilize the BiFC
• Load cells onto multi-well plates
• Each well serves as a Pixel

<img width="600" height="200" src="" alt="1">

Degradation of cellular proteins

• Utilize the Ubiquitin/26S proteasome system
• Linking the voltage switch with E1/E2/E3 protein
• Control degradation rate of cellular proteins by controlling ubiquitinylation rate

Study genes using protein complementation

• Split genes into two parts, and insert into our system
• Use voltage switch to control the split and refold of a gene.
• Possible tool for the screening of new protein complementation compatible proteins.

<img width="600" height="200" src="" alt="2">

Study Membrane Potential of Organelles

• Target voltage switch system with BiFC onto the membrane of organelles interested
• Obtain fluorescent signal
• Estimate membrane potential

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