Team:Hong Kong-CUHK/project-4.html

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Voltage Switch is a response system triggered by a change of potential across the bacterial inner membrane. Components of this system include the transmembrane voltage sensor peptide originated from potassium ion channels, polyproline linker in the inner membrane, glycine serine linker, PDZ domain and PDZ ligand in the periplasm, and glycine serine linker and effectors in the cytosol.

1. Components of Voltage Switch System

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<stong> Voltage Sensor Peptide
Voltage sensor peptide, originated from the archaea Aeropyrum pernix, is a protein domain found in a voltage-dependent potassium ion channel. Previous studies suggested that the domain could translocate within the membrane upon membrane potential change [10, 11]. This helix peptide received its voltage-sensing ability from its four positively-charged arginine residues, and high hydrophobicity from its numerous leucine residues.</stong>

PDZ Domain and PDZ Ligand
This is a dimer found in Mus musculus, and were used by the 2012 SJTU iGEM team as a membrane scaffolding tool. The PDZ dimer provides the necessary linkage of the two fusion proteins in the voltage switch system.

Polyproline Linker
Polyproline linker is a pure proline rigid rod. Its rigidity is due to the limited rotation of peptide bonds in proline, a ring-shaped amino acid. Also, proline does not contain any hydrophilic functional group, which makes the linker hydrophobic and thus able to stay within the inner membrane.

Glycine Serine Linker
This is a hydrophilic flexible linker which links PDZ dimer with voltage sensor peptides. It also links the polyproline linkers in the membrane with the effectors in the cytosol.

Effectors
These effectors can be changed accordingly. In our project, we chose laccase and dioxygenase for BaP degradation. We also planned to test the system with bimolecular fluorescence complementation (BiFC) dimerization. To become functional, the PDZ domain and PDZ ligand bind the two protein fragments together, forming a stable dimer.

2. Proposed Mechanism of Switching “ON” and “OFF” in the System

Switching “OFF”
When no external charge is applied, the two effectors are combined, as the repulsion force between the two positively-charged voltage sensor peptide is relatively smaller than the dimer effect. The two voltage sensor peptides are vertically parallel to each other.

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Switching “ON”
When negative charge is applied into the system, the two effectors are separated. As the potential across the membrane is inverted, there is an attractive force between negatively-charged periplasm and positively-charged voltage sensor peptide, and vice versa for cytosol and voltage sensor peptide. Together, these two forces pull the two voltage sensor peptides from vertical position to more horizontal, which causes the separation of the effectors.

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