Wet lab

Approach

Our experimental approach can be summarized as follows:

Click to enlarge

Number	Gene	Organism	pIGA (pDONR+GOI in E.coli)	pIGB (pDEST+GOI for AH109)	AIG (derived from AH109)	Y2H (interaction partners)
1	mp65	N/A	X	X	X	X
2	tos1	N/A	X	X	X	X
3	sim1	N/A	X	X	X	X
4	ssr1	N/A	X	X	X	X
5	pir4	N/A	X	X	X	X
6	scw4	N/A	X	X	X	X
7	pir3	N/A	X	X	N/A	N/A
8	utr2	N/A	X	X	N/A	N/A
9	crf2full	A. fumigatus	X	X	X	X
10	crf2act	A. fumigatus	X	X	X	X
11	bglEno	A. fumigatus	X	N/A	N/A	N/A
12	ecm33	A. fumigatus	X	X	X	X
13	sun1	A. fumigatus	X	X	X	X
14	als1	N/A	X	X	X	X
15	als3	N/A	X	X	X	X
16	rodA	A. fumigatus	X	X	N/A	N/A
17	rodA	A. nidulans	X	X	X	X
18	xlnA	A. nidulans	X	X	X	X
19	npc2	A. nidulans	X	X	X	X
20	sho1	A. nidulans	X	X	X	X
21	MID2	S. cerevisiae	X	X	X	X
22	SED1	S. cerevisiae	X	X	X	X
23	prm1 B	A. fumigatus	X	X	N/A	N/A
24	crf1	A. fumigatus	X	X	X	X
25	ama1	T. gondii	X	X	X	X
26	eglC	A. fumigatus	X	N/A	N/A	N/A
27	CWP1	S. cerevisiae	X	X	N/A	N/A
28	CWP2	S. cerevisiae	X	X	N/A	N/A
29	TIR4	S. cerevisiae	X	N/A	N/A	N/A
30	rom5	T. gondii	X	N/A	N/A	N/A
31	rom4	T. gondii	X	N/A	N/A	N/A
32	gra7	T. gondii	X	N/A	N/A	N/A
33	eglC	A. nidulans	X	N/A	N/A	N/A
34	TIR1	S. cerevisiae	X	X	N/A	N/A
35	bglEex	A. fumigatus	X	X	N/A	N/A

Number	Gene	Organism	pIGA (pDONR+GOI in E.coli)	pIGB (pDEST+GOI for AH109)	AIG (derived from AH109)	Y2H (interaction partners)	pIGZ (p1170 from diploid cells for E.coli)	pIGY (p1170 from E.coli for Y187)	YIG (derived from Y187)	PepTag
1	X
1	X
X
1	X
X
X
1	X
X
X

2	X
2	X
X
2	X
X
X
2	X
X
X

3	X
3	X
X
3	X
X
X
3	X
X
X

4	X
4	X
X
4	X
X
X
4	X
X
X
X
5	X
5	X
X
5	X
X
X
5	X
X
X
X
6	X
6	X
X
6	X
X
X
6	X
X
X

7	N/A
7	N/A
N/A
7	N/A
N/A
N/A
7	N/A
N/A
N/A

8	N/A
8	N/A
N/A
8	N/A
N/A
N/A
8	N/A
N/A
N/A

9	X
9	X
X
9	X
X
X
9	X
X
X

10	X
10	X
X
10	X
X
X
10	X
X
X

11	N/A
11	N/A
N/A
11	N/A
N/A
N/A
11	N/A
N/A
N/A

12	X
12	X
X
12	X
X
X
12	X
X
X

13	X
13	X
X
13	X
X
X
13	X
X
X
X
14	X
14	X
X
14	X
X
X
14	X
X
X

15	X
15	X
X
15	X
X
X
15	X
X
X

16	N/A
16	N/A
N/A
16	N/A
N/A
N/A
16	N/A
N/A
N/A

17	X
17	X
X
17	X
X
X
17	X
X
X

18	N/A
18	N/A
N/A
18	N/A
N/A
N/A
18	N/A
N/A
N/A

19	X
19	X
X
19	X
X
X
19	X
X
X

20	N/A
20	N/A
N/A
20	N/A
N/A
N/A
20	N/A
N/A
N/A

21	X
21	X
X
21	X
X
X
21	X
X
X

22	N/A
22	N/A
N/A
22	N/A
N/A
N/A
22	N/A
N/A
N/A

23	N/A
23	N/A
N/A
23	N/A
N/A
N/A
23	N/A
N/A
N/A

24	X
24	X
X
24	X
X
X
24	X
X
X

25	X
25	X
X
25	X
X
X
25	X
X
X

26	N/A
26	N/A
N/A
26	N/A
N/A
N/A
26	N/A
N/A
N/A

27	N/A
27	N/A
N/A
27	N/A
N/A
N/A
27	N/A
N/A
N/A

28	N/A
28	N/A
N/A
28	N/A
N/A
N/A
28	N/A
N/A
N/A

29	N/A
29	N/A
N/A
29	N/A
N/A
N/A
29	N/A
N/A
N/A

30	N/A
30	N/A
N/A
30	N/A
N/A
N/A
30	N/A
N/A
N/A

31	N/A
31	N/A
N/A
31	N/A
N/A
N/A
31	N/A
N/A
N/A

32	N/A
32	N/A
N/A
32	N/A
N/A
N/A
32	N/A
N/A
N/A

33	N/A
33	N/A
N/A
33	N/A
N/A
N/A
33	N/A
N/A
N/A

34	N/A
34	N/A
N/A
34	N/A
N/A
N/A
34	N/A
N/A
N/A

35	N/A
35	N/A
N/A
35	N/A
N/A
N/A
35	N/A
N/A
N/A

Number	Gene	Organism	pIGA (pDONR+GOI in E.coli)	pIGB (pDEST+GOI for AH109)	AIG (derived from AH109)	Y2H (interaction partners)	pIGZ (p1170 from diploid cells for E.coli)	pIGY (p1170 from E.coli for Y187)	YIG (derived from Y187)	PepTag
1	X
1	X
X
1	X
X
X
1	X
X
X

2	X
2	X
X
2	X
X
X
2	X
X
X

3	X
3	X
X
3	X
X
X
3	X
X
X

4	X
4	X
X
4	X
X
X
4	X
X
X
X
5	X
5	X
X
5	X
X
X
5	X
X
X
X
6	X
6	X
X
6	X
X
X
6	X
X
X

7	N/A
7	N/A
N/A
7	N/A
N/A
N/A
7	N/A
N/A
N/A

8	N/A
8	N/A
N/A
8	N/A
N/A
N/A
8	N/A
N/A
N/A

9	X
9	X
X
9	X
X
X
9	X
X
X

10	X
10	X
X
10	X
X
X
10	X
X
X

11	N/A
11	N/A
N/A
11	N/A
N/A
N/A
11	N/A
N/A
N/A

12	X
12	X
X
12	X
X
X
12	X
X
X

13	X
13	X
X
13	X
X
X
13	X
X
X
X
14	X
14	X
X
14	X
X
X
14	X
X
X

15	X
15	X
X
15	X
X
X
15	X
X
X

16	N/A
16	N/A
N/A
16	N/A
N/A
N/A
16	N/A
N/A
N/A

17	X
17	X
X
17	X
X
X
17	X
X
X

18	N/A
18	N/A
N/A
18	N/A
N/A
N/A
18	N/A
N/A
N/A

19	X
19	X
X
19	X
X
X
19	X
X
X

20	N/A
20	N/A
N/A
20	N/A
N/A
N/A
20	N/A
N/A
N/A

21	X
21	X
X
21	X
X
X
21	X
X
X

22	N/A
22	N/A
N/A
22	N/A
N/A
N/A
22	N/A
N/A
N/A

23	N/A
23	N/A
N/A
23	N/A
N/A
N/A
23	N/A
N/A
N/A

24	X
24	X
X
24	X
X
X
24	X
X
X

25	X
25	X
X
25	X
X
X
25	X
X
X

26	N/A
26	N/A
N/A
26	N/A
N/A
N/A
26	N/A
N/A
N/A

27	N/A
27	N/A
N/A
27	N/A
N/A
N/A
27	N/A
N/A
N/A

28	N/A
28	N/A
N/A
28	N/A
N/A
N/A
28	N/A
N/A
N/A

29	N/A
29	N/A
N/A
29	N/A
N/A
N/A
29	N/A
N/A
N/A

30	N/A
30	N/A
N/A
30	N/A
N/A
N/A
30	N/A
N/A
N/A

31	N/A
31	N/A
N/A
31	N/A
N/A
N/A
31	N/A
N/A
N/A

32	N/A
32	N/A
N/A
32	N/A
N/A
N/A
32	N/A
N/A
N/A

33	N/A
33	N/A
N/A
33	N/A
N/A
N/A
33	N/A
N/A
N/A

34	N/A
34	N/A
N/A
34	N/A
N/A
N/A
34	N/A
N/A
N/A

35	N/A
35	N/A
N/A
35	N/A
N/A
N/A
35	N/A
N/A
N/A

Number	Gene	Organism	pIGA (pDONR+GOI in E.coli)	pIGB (pDEST+GOI for AH109)	AIG (derived from AH109)	Y2H (interaction partners)	pIGZ (p1170 from diploid cells for E.coli)	pIGY (p1170 from E.coli for Y187)	YIG (derived from Y187)	PepTag
1	X
1	X
X
1	X
X
X
1	X
X
X

2	X
2	X
X
2	X
X
X
2	X
X
X

3	X
3	X
X
3	X
X
X
3	X
X
X

4	X
4	X
X
4	X
X
X
4	X
X
X
X
5	X
5	X
X
5	X
X
X
5	X
X
X
X
6	X
6	X
X
6	X
X
X
6	X
X
X

7	N/A
7	N/A
N/A
7	N/A
N/A
N/A
7	N/A
N/A
N/A

8	N/A
8	N/A
N/A
8	N/A
N/A
N/A
8	N/A
N/A
N/A

9	X
9	X
X
9	X
X
X
9	X
X
X

10	X
10	X
X
10	X
X
X
10	X
X
X

11	N/A
11	N/A
N/A
11	N/A
N/A
N/A
11	N/A
N/A
N/A

12	X
12	X
X
12	X
X
X
12	X
X
X

13	X
13	X
X
13	X
X
X
13	X
X
X
X
14	X
14	X
X
14	X
X
X
14	X
X
X

15	X
15	X
X
15	X
X
X
15	X
X
X

16	N/A
16	N/A
N/A
16	N/A
N/A
N/A
16	N/A
N/A
N/A

17	X
17	X
X
17	X
X
X
17	X
X
X

18	N/A
18	N/A
N/A
18	N/A
N/A
N/A
18	N/A
N/A
N/A

19	X
19	X
X
19	X
X
X
19	X
X
X

20	N/A
20	N/A
N/A
20	N/A
N/A
N/A
20	N/A
N/A
N/A

21	X
21	X
X
21	X
X
X
21	X
X
X

22	N/A
22	N/A
N/A
22	N/A
N/A
N/A
22	N/A
N/A
N/A

23	N/A
23	N/A
N/A
23	N/A
N/A
N/A
23	N/A
N/A
N/A

24	X
24	X
X
24	X
X
X
24	X
X
X

25	X
25	X
X
25	X
X
X
25	X
X
X

26	N/A
26	N/A
N/A
26	N/A
N/A
N/A
26	N/A
N/A
N/A

27	N/A
27	N/A
N/A
27	N/A
N/A
N/A
27	N/A
N/A
N/A

28	N/A
28	N/A
N/A
28	N/A
N/A
N/A
28	N/A
N/A
N/A

29	N/A
29	N/A
N/A
29	N/A
N/A
N/A
29	N/A
N/A
N/A

30	N/A
30	N/A
N/A
30	N/A
N/A
N/A
30	N/A
N/A
N/A

31	N/A
31	N/A
N/A
31	N/A
N/A
N/A
31	N/A
N/A
N/A

32	N/A
32	N/A
N/A
32	N/A
N/A
N/A
32	N/A
N/A
N/A

33	N/A
33	N/A
N/A
33	N/A
N/A
N/A
33	N/A
N/A
N/A

34	N/A
34	N/A
N/A
34	N/A
N/A
N/A
34	N/A
N/A
N/A

35	N/A
35	N/A
N/A
35	N/A
N/A
N/A
35	N/A
N/A
N/A

Number	Gene	Organism	pIGA (pDONR+GOI in E.coli)	pIGB (pDEST+GOI for AH109)	AIG (derived from AH109)	Y2H (interaction partners)	pIGZ (p1170 from diploid cells for E.coli)	pIGY (p1170 from E.coli for Y187)	YIG (derived from Y187)	PepTag
1	X
1	X
X
1	X
X
X
1	X
X
X

2	X
2	X
X
2	X
X
X
2	X
X
X

3	X
3	X
X
3	X
X
X
3	X
X
X

4	X
4	X
X
4	X
X
X
4	X
X
X
X
5	X
5	X
X
5	X
X
X
5	X
X
X
X
6	X
6	X
X
6	X
X
X
6	X
X
X

7	N/A
7	N/A
N/A
7	N/A
N/A
N/A
7	N/A
N/A
N/A

8	N/A
8	N/A
N/A
8	N/A
N/A
N/A
8	N/A
N/A
N/A

9	X
9	X
X
9	X
X
X
9	X
X
X

10	X
10	X
X
10	X
X
X
10	X
X
X

11	N/A
11	N/A
N/A
11	N/A
N/A
N/A
11	N/A
N/A
N/A

12	X
12	X
X
12	X
X
X
12	X
X
X

13	X
13	X
X
13	X
X
X
13	X
X
X
X
14	X
14	X
X
14	X
X
X
14	X
X
X

15	X
15	X
X
15	X
X
X
15	X
X
X

16	N/A
16	N/A
N/A
16	N/A
N/A
N/A
16	N/A
N/A
N/A

17	X
17	X
X
17	X
X
X
17	X
X
X

18	N/A
18	N/A
N/A
18	N/A
N/A
N/A
18	N/A
N/A
N/A

19	X
19	X
X
19	X
X
X
19	X
X
X

20	N/A
20	N/A
N/A
20	N/A
N/A
N/A
20	N/A
N/A
N/A

21	X
21	X
X
21	X
X
X
21	X
X
X

22	N/A
22	N/A
N/A
22	N/A
N/A
N/A
22	N/A
N/A
N/A

23	N/A
23	N/A
N/A
23	N/A
N/A
N/A
23	N/A
N/A
N/A

24	X
24	X
X
24	X
X
X
24	X
X
X

25	X
25	X
X
25	X
X
X
25	X
X
X

26	N/A
26	N/A
N/A
26	N/A
N/A
N/A
26	N/A
N/A
N/A

27	N/A
27	N/A
N/A
27	N/A
N/A
N/A
27	N/A
N/A
N/A

28	N/A
28	N/A
N/A
28	N/A
N/A
N/A
28	N/A
N/A
N/A

29	N/A
29	N/A
N/A
29	N/A
N/A
N/A
29	N/A
N/A
N/A

30	N/A
30	N/A
N/A
30	N/A
N/A
N/A
30	N/A
N/A
N/A

31	N/A
31	N/A
N/A
31	N/A
N/A
N/A
31	N/A
N/A
N/A

32	N/A
32	N/A
N/A
32	N/A
N/A
N/A
32	N/A
N/A
N/A

33	N/A
33	N/A
N/A
33	N/A
N/A
N/A
33	N/A
N/A
N/A

34	N/A
34	N/A
N/A
34	N/A
N/A
N/A
34	N/A
N/A
N/A

35	N/A
35	N/A
N/A
35	N/A
N/A
N/A
35	N/A
N/A
N/A