Team:Goettingen/project overview/project wetlab
From 2014.igem.org
Project
Wet lab
Approach
Our experimental approach can be summarized as follows:
1. Organism and surface protein selection
2. Surface protein amplification and cloning
3. Yeast two-hybrid assay with an existing peptide library
4. Peptide functionalization
Results summary
Number | Gene | Organism | pIGA (pDONR+GOI in E.coli) | pIGB (pDEST+GOI for AH109) | AIG (derived from AH109) | Y2H (interaction partners) | pIGZ (p1170 from diploid cells for E.coli) | pIGY (p1170 from E.coli for Y187) | YIG (derived from Y187) | PepTag |
---|---|---|---|---|---|---|---|---|---|---|
1 | mp65 | N/A | X | X | X | X | X | X | X | |
2 | tos1 | N/A | X | X | X | X | X | X | X | |
3 | sim1 | N/A | X | X | X | X | X | X | X | |
4 | ssr1 | N/A | X | X | X | X | X | X | X | X |
5 | pir4 | N/A | X | X | X | X | X | X | X | X |
6 | scw4 | N/A | X | X | X | X | X | X | X | |
7 | pir3 | N/A | X | X | N/A | N/A | N/A | N/A | N/A | |
8 | utr2 | N/A | X | X | N/A | N/A | N/A | N/A | N/A | |
9 | crf2full | A. fumigatus | X | X | X | X | X | X | X | |
10 | crf2act | A. fumigatus | X | X | X | X | X | X | X | |
11 | bglEno | A. fumigatus | X | N/A | N/A | N/A | N/A | N/A | N/A | |
12 | ecm33 | A. fumigatus | X | X | X | X | X | X | X | |
13 | sun1 | A. fumigatus | X | X | X | X | X | X | X | X |
14 | als1 | N/A | X | X | X | X | X | X | X | |
15 | als3 | N/A | X | X | X | X | X | X | X | |
16 | rodA | A. fumigatus | X | X | N/A | N/A | N/A | N/A | N/A | |
17 | rodA | A. nidulans | X | X | X | X | X | X | X | |
18 | xlnA | A. nidulans | X | X | X | X | N/A | N/A | N/A | |
19 | npc2 | A. nidulans | X | X | X | X | X | X | X | |
20 | sho1 | A. nidulans | X | X | X | X | N/A | N/A | N/A | |
21 | MID2 | S. cerevisiae | X | X | X | X | X | X | X | |
22 | SED1 | S. cerevisiae | X | X | X | X | N/A | N/A | N/A | |
23 | prm1 B | A. fumigatus | X | X | N/A | N/A | N/A | N/A | N/A | |
24 | crf1 | A. fumigatus | X | X | X | X | X | X | X | |
25 | ama1 | T. gondii | X | X | X | X | X | X | X | |
26 | eglC | A. fumigatus | X | N/A | N/A | N/A | N/A | N/A | N/A | |
27 | CWP1 | S. cerevisiae | X | X | N/A | N/A | N/A | N/A | N/A | |
28 | CWP2 | S. cerevisiae | X | X | N/A | N/A | N/A | N/A | N/A | |
29 | TIR4 | S. cerevisiae | X | N/A | N/A | N/A | N/A | N/A | N/A | |
30 | rom5 | T. gondii | X | N/A | N/A | N/A | N/A | N/A | N/A | |
31 | rom4 | T. gondii | X | N/A | N/A | N/A | N/A | N/A | N/A | |
32 | gra7 | T. gondii | X | N/A | N/A | N/A | N/A | N/A | N/A | |
33 | eglC | A. nidulans | X | N/A | N/A | N/A | N/A | N/A | N/A | |
34 | TIR1 | S. cerevisiae | X | X | N/A | N/A | N/A | N/A | N/A | |
35 | bglEex | A. fumigatus | X | X | N/A | N/A | N/A | N/A | N/A |