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Team:Goettingen/project overview/project biobrick
From 2014.igem.org
Project
BioBricks
Biobricks are standard biological parts that are DNA sequences of defined structure and function. They share a common interface and are designed to be composed and incorporated into living cells such as E.coli to construct new biological systems. Every brick consists of a prefix sequence, the DNA part and a suffix sequence.
These are the biobricks that we built:
| Code | Name | Description | Length (bp) |
|---|---|---|---|
| K1430000 | Yeast 2-micron | 1343 | |
| K1430001 | Yeast LEU2 gene | Selective marker for auxotrophic strains of yeast after transforamtion with plasmid that contains this gene. | 1095 |
| K1430002 | TRP1 gene from yeast | This part is a fusion of promoter and terminator in front/back of the TRP1 of Saccharomyces cerevisiae | 1501 |
| K1430003 | Yeast TRIP1 gene | Selective marker for auxotrophic strains of yeast after transforamtion with plasmid that contains this gene. | 675 |
| K1430004 | LEU2 gene from yeast | This part is a fusion of promoter and terminator in front/back of the LEU2 of S. cerevisiae | 1921 |
| K1430005 | GFP tag for yeast proteins | The BamHI site in front of the GFP allows to tag proteins in yeast with GFP. | 2953 |
| K1430006 | mRFP tag for yeast proteins | The BamHI site in front of the mRFP allows to tag proteins in yeast with mRFP. | 3334 |
| K1430007 | Tool to tag yeast proteins with GFP | Use this plasmid to enter your protein of interest into BamH1 or BgIII restriction site. It is tagged to GFP. The plasmid selection is done by TRP1. | 4685 |
| K1430008 | Tool to tag yeast proteins with mRFP | Use this plasmid to enter your protein of interest into BamH1 or BgIII restriction site. It is tagged to mRFP. The plasmid selection is done by LEU2. | 4304 |
