Team:SF Bay Area DIYbio/Notebook

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Notebook

General Protocols

Electra Cloning Protocol
Media:Zymo E. coli competent cells transformation protocol.pdf
Media:D4025i.pdf (Zyppy plasmid midiprep protocol)
Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf

General Documentation Information

Real Vegan Cheese Notebook Documentation Guidelines
Real Vegan Cheese Notebook Training Sign-off

June 2014

CR_062214_humanKappaCasein_001
August 2014 CLONE_082514_humKappaCasein(Kex+)
Growth Curve Experiment
September 2014 Cloning 7 casein gBlocks, 12Sep2014 and on
Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S
Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014
October 2014 Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2
Cloning human alpha casein S1, 04Oct2014 and on
Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014

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