At the iGEM meetup in Munich, members of both of our teams realized that our projects share a common objective: Analyzing 2-dimensional, visual signals. For the next months we stayed in contact and developed a concept to encode information in 384-well plates in the form of datamatrix codes.
On September 2nd, Michael spontaneously took a train to Freiburg and we meet face-to-face for several hours to exchange details on our cooperation and general iGEM experiences.
Data Matrix Masks for illumination
We decided to use masks to locally induce the cells in 384-well plates in a datamatrix pattern. To enable us to quickly design masks for different data to be encoded, we wrote a software to easily generate SVG-files for the lasercutter.
After a string was entered in the designer, a SVG file can be saved and used for lasercutting the mask:
Get the DatamatrixMaskDesigner to make you own MammoMatrixes! (requires Windows 7/8)
You can also download the full source code and compile or modify it with Visual Studio (Express) 2013.
For the first experiments we cut two different datamatrix patterns in a polypropylen foil. The masks can be used to cover the plates from the top, or from the bottom.
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