Team:Paris Saclay/Notebook/August/22
From 2014.igem.org
Contents |
Friday 22nd August
Lab work
D - Lemon Scent
pPSI digestion
40µl pPSI
2µl H2O
2µL PacI
5µL FAST digest buffer
pPSI dephosphorylation
After 2 hours, add 1.5µL of Alkaline Phosphatase (AP) and let it 1 hour.
Then, inactivation of AP at 65°C during 15 minutes.
We've made a electrophoresis to chech the digestion.
(photo)
Ligation of LS/GS/PS in pPSI
For each synthase,put:
- 10 µL of synthase
- 2 µL of pPSI
- 2 µL of buffer
- 5 µL of water
- 1 µL of ligase
And let it in the freezer during 3 hours.