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Team:Paris Saclay/Notebook/July/8
From 2014.igem.org
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=Tuesday 8th July= | =Tuesday 8th July= | ||
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==Lab work== | ==Lab work== | ||
| - | + | ====Rehydration of the following biobricks:==== | |
| - | ==== | + | |
'''Sean''' | '''Sean''' | ||
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* BBa_J23114(AmpR) | * BBa_J23114(AmpR) | ||
| - | + | Protocol: | |
| - | Protocol: | + | |
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# Pierce foil cover of well containing biobrick of interest. | # Pierce foil cover of well containing biobrick of interest. | ||
# Add 10 μl of water in well. | # Add 10 μl of water in well. | ||
# Transfer contents of well into a 1.5 ml microcentrifuge tube. | # Transfer contents of well into a 1.5 ml microcentrifuge tube. | ||
| - | ==== | + | ====Transformation in DH5α of the following biobricks:==== |
'''Mathieu & Maher''' | '''Mathieu & Maher''' | ||
* BBa_B0015(CmR) | * BBa_B0015(CmR) | ||
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* Control + : pUC18 (20ng) | * Control + : pUC18 (20ng) | ||
* Control - | * Control - | ||
| - | |||
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells Protocol] | ||
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NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution. | NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution. | ||
| - | ==== | + | ====Plasmid DNA preparation:==== |
| - | + | ||
'''Romain & Sean''' | '''Romain & Sean''' | ||
Plasmids used: | Plasmids used: | ||
* pJBEI-6409 | * pJBEI-6409 | ||
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''E. coli'' strain used: | ''E. coli'' strain used: | ||
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol] | ||
| - | + | {{Team:Paris_Saclay/notebook_footer}} | |
Revision as of 08:50, 7 August 2014
Contents |
Tuesday 8th July
Lab work
Rehydration of the following biobricks:
Sean
- BBa_B0015(CmR)
- BBa_J23119(CmR)
- BBa_K731020(CmR)
- BBa_K206000(CmR)
- BBa_J04500(CmR)
- BBa_J23100(AmpR)
- BBa_J23106(AmpR)
- BBa_J23114(AmpR)
Protocol:
- Pierce foil cover of well containing biobrick of interest.
- Add 10 μl of water in well.
- Transfer contents of well into a 1.5 ml microcentrifuge tube.
Transformation in DH5α of the following biobricks:
Mathieu & Maher
- BBa_B0015(CmR)
- BBa_J23119(CmR)
- BBa_K731020(CmR)
- BBa_K206000(CmR)
- BBa_J04500(CmR)
- BBa_J23100(AmpR)
- BBa_J23106(AmpR)
- BBa_J23114(AmpR)
- Control + : pUC18 (20ng)
- Control -
NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution.
Plasmid DNA preparation:
Romain & Sean
Plasmids used:
- pJBEI-6409
E. coli strain used:
- XL1-Blue
