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Team:Goettingen/project overview/project wetlab
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<center><table class="table1" style="width:700px"> | <center><table class="table1" style="width:700px"> | ||
<tr style="font-weight:bold;"> | <tr style="font-weight:bold;"> | ||
| - | |||
<th>Gene</td> | <th>Gene</td> | ||
<th>Organism</td> | <th>Organism</td> | ||
</tr> | </tr> | ||
| - | <tr | + | <tr><td><i>mp65</i></td><td>N/A</td></tr> |
| - | <tr | + | <tr><td><i>tos1</i></td><td>N/A</td></tr> |
| - | <tr | + | <tr><td><i>sim1</i></td><td>N/A</td></tr> |
| - | <tr | + | <tr><td><i>ssr1</i></td><td>N/A</td></tr> |
| - | <tr | + | <tr><td><i>pir4</i></td><td>N/A</td></tr> |
| - | <tr | + | <tr><td><i>scw4</i></td><td>N/A</td></tr> |
| - | <tr | + | <tr><td><i>pir3</i></td><td>N/A</td></tr> |
| - | <tr | + | <tr><td><i>utr2</i></td><td>N/A</td></tr> |
| - | <tr | + | <tr><td><i>crf2full</i></td><td><i>A. fumigatus</i></td></tr> |
| - | <tr | + | <tr><td><i>crf2act</i></td><td><i>A. fumigatus</i></td></tr> |
| - | <tr | + | <tr><td><i>bgleno</i></td><td><i>A. fumigatus</i></td></tr> |
| - | <tr | + | <tr><td><i>ecm33</i></td><td><i>A. fumigatus</i></td></tr> |
| - | <tr | + | <tr><td><i>sun1</i></td><td><i>A. fumigatus</i></td></tr> |
| - | <tr | + | <tr><td><i>als1</i></td><td>N/A</td></tr> |
| - | <tr | + | <tr><td><i>als3</i></td><td>N/A</td></tr> |
| - | <tr | + | <tr><td><i>roda</i></td><td><i>A. fumigatus</i></td></tr> |
| - | <tr | + | <tr><td><i>roda</i></td><td><i>A. nidulans</i></td></tr> |
| - | <tr | + | <tr><td><i>xlna</i></td><td><i>A. nidulans</i></td></tr> |
| - | <tr | + | <tr><td><i>npc2</i></td><td><i>A. nidulans</i></td></tr> |
| - | <tr | + | <tr><td><i>sho1</i></td><td><i>A. nidulans</i></td></tr> |
| - | <tr | + | <tr><td><i>mid2</i></td><td><i>S. cerevisiae</i></td></tr> |
| - | <tr | + | <tr><td><i>sed1</i></td><td><i>S. cerevisiae</i></td></tr> |
| - | <tr | + | <tr><td><i>prm1 b</i></td><td><i>A. fumigatus</i></td></tr> |
| - | <tr | + | <tr><td><i>crf1</i></td><td><i>A. fumigatus</i></td></tr> |
| - | <tr | + | <tr><td><i>ama1</i></td><td><i>T. gondii</i></td></tr> |
| - | <tr | + | <tr><td><i>eglc</i></td><td><i>A. fumigatus</i></td></tr> |
| - | <tr | + | <tr><td><i>cwp1</i></td><td><i>S. cerevisiae</i></td></tr> |
| - | <tr | + | <tr><td><i>cwp2</i></td><td><i>S. cerevisiae</i></td></tr> |
| - | <tr | + | <tr><td><i>tir4</i></td><td><i>S. cerevisiae</i></td></tr> |
| - | <tr | + | <tr><td><i>rom5</i></td><td><i>T. gondii</i></td></tr> |
| - | <tr | + | <tr><td><i>rom4</i></td><td><i>T. gondii</i></td></tr> |
| - | <tr | + | <tr><td><i>gra7</i></td><td><i>T. gondii</i></td></tr> |
| - | <tr | + | <tr><td><i>eglc</i></td><td><i>A. nidulans</i></td></tr> |
| - | <tr | + | <tr><td><i>tir1</i></td><td><i>S. cerevisiae</i></td></tr> |
| - | <tr | + | <tr><td><i>bgleex</i></td><td><i>A. fumigatus</i></td></tr> |
</table></center> | </table></center> | ||
| - | |||
| - | |||
Revision as of 10:17, 17 October 2014
Project
Wet lab
Approach
Our experimental approach can be summarized as follows:
1. Organisms and surface proteins selection
2. Surface protein amplification and cloning
3. Yeast two-hybrid assay with an existing peptide library
4. Peptide functionalization
Results summary
These are the genes and the organisms we selected:
| Gene | Organism |
|---|---|
| mp65 | N/A |
| tos1 | N/A |
| sim1 | N/A |
| ssr1 | N/A |
| pir4 | N/A |
| scw4 | N/A |
| pir3 | N/A |
| utr2 | N/A |
| crf2full | A. fumigatus |
| crf2act | A. fumigatus |
| bgleno | A. fumigatus |
| ecm33 | A. fumigatus |
| sun1 | A. fumigatus |
| als1 | N/A |
| als3 | N/A |
| roda | A. fumigatus |
| roda | A. nidulans |
| xlna | A. nidulans |
| npc2 | A. nidulans |
| sho1 | A. nidulans |
| mid2 | S. cerevisiae |
| sed1 | S. cerevisiae |
| prm1 b | A. fumigatus |
| crf1 | A. fumigatus |
| ama1 | T. gondii |
| eglc | A. fumigatus |
| cwp1 | S. cerevisiae |
| cwp2 | S. cerevisiae |
| tir4 | S. cerevisiae |
| rom5 | T. gondii |
| rom4 | T. gondii |
| gra7 | T. gondii |
| eglc | A. nidulans |
| tir1 | S. cerevisiae |
| bgleex | A. fumigatus |
The results for all the genes we selected are summarized in this file.
