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Team:Goettingen/project overview/project wetlab
From 2014.igem.org
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<h2 id="wlresults">Results summary</h2> | <h2 id="wlresults">Results summary</h2> | ||
| - | <p>The results for all the genes we selected are summarized in this <a href="https://static.igem.org/mediawiki/2014/6/60/Goettingen2014-NumberS.pdf"> | + | |
| + | <p>These are the genes and the organisms we selected:</p><br /> | ||
| + | |||
| + | <center><table class="table1" style="width:700px"> | ||
| + | <tr style="font-weight:bold;"> | ||
| + | <th>Number</td> | ||
| + | <th>Gene</td> | ||
| + | <th>Organism</td> | ||
| + | </tr> | ||
| + | <tr><td>1</td><td><i>mp65</i></td><td>N/A</td></tr> | ||
| + | <tr><td>2</td><td><i>tos1</i></td><td>N/A</td></tr> | ||
| + | <tr><td>3</td><td><i>sim1</i></td><td>N/A</td></tr> | ||
| + | <tr><td>4</td><td><i>ssr1</i></td><td>N/A</td></tr> | ||
| + | <tr><td>5</td><td><i>pir4</i></td><td>N/A</td></tr> | ||
| + | <tr><td>6</td><td><i>scw4</i></td><td>N/A</td></tr> | ||
| + | <tr><td>7</td><td><i>pir3</i></td><td>N/A</td></tr> | ||
| + | <tr><td>8</td><td><i>utr2</i></td><td>N/A</td></tr> | ||
| + | <tr><td>9</td><td><i>crf2full</i></td><td><i>A. fumigatus</i></td></tr> | ||
| + | <tr><td>10</td><td><i>crf2act</i></td><td><i>A. fumigatus</i></td></tr> | ||
| + | <tr><td>11</td><td><i>bgleno</i></td><td><i>A. fumigatus</i></td></tr> | ||
| + | <tr><td>12</td><td><i>ecm33</i></td><td><i>A. fumigatus</i></td></tr> | ||
| + | <tr><td>13</td><td><i>sun1</i></td><td><i>A. fumigatus</i></td></tr> | ||
| + | <tr><td>14</td><td><i>als1</i></td><td>N/A</td></tr> | ||
| + | <tr><td>15</td><td><i>als3</i></td><td>N/A</td></tr> | ||
| + | <tr><td>16</td><td><i>roda</i></td><td><i>A. fumigatus</i></td></tr> | ||
| + | <tr><td>17</td><td><i>roda</i></td><td><i>A. nidulans</i></td></tr> | ||
| + | <tr><td>18</td><td><i>xlna</i></td><td><i>A. nidulans</i></td></tr> | ||
| + | <tr><td>19</td><td><i>npc2</i></td><td><i>A. nidulans</i></td></tr> | ||
| + | <tr><td>20</td><td><i>sho1</i></td><td><i>A. nidulans</i></td></tr> | ||
| + | <tr><td>21</td><td><i>mid2</i></td><td><i>S. cerevisiae</i></td></tr> | ||
| + | <tr><td>22</td><td><i>sed1</i></td><td><i>S. cerevisiae</i></td></tr> | ||
| + | <tr><td>23</td><td><i>prm1 b</i></td><td><i>A. fumigatus</i></td></tr> | ||
| + | <tr><td>24</td><td><i>crf1</i></td><td><i>A. fumigatus</i></td></tr> | ||
| + | <tr><td>25</td><td><i>ama1</i></td><td><i>T. gondii</i></td></tr> | ||
| + | <tr><td>26</td><td><i>eglc</i></td><td><i>A. fumigatus</i></td></tr> | ||
| + | <tr><td>27</td><td><i>cwp1</i></td><td><i>S. cerevisiae</i></td></tr> | ||
| + | <tr><td>28</td><td><i>cwp2</i></td><td><i>S. cerevisiae</i></td></tr> | ||
| + | <tr><td>29</td><td><i>tir4</i></td><td><i>S. cerevisiae</i></td></tr> | ||
| + | <tr><td>30</td><td><i>rom5</i></td><td><i>T. gondii</i></td></tr> | ||
| + | <tr><td>31</td><td><i>rom4</i></td><td><i>T. gondii</i></td></tr> | ||
| + | <tr><td>32</td><td><i>gra7</i></td><td><i>T. gondii</i></td></tr> | ||
| + | <tr><td>33</td><td><i>eglc</i></td><td><i>A. nidulans</i></td></tr> | ||
| + | <tr><td>34</td><td><i>tir1</i></td><td><i>S. cerevisiae</i></td></tr> | ||
| + | <tr><td>35</td><td><i>bgleex</i></td><td><i>A. fumigatus</i></td></tr> | ||
| + | </table></center> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <p>The results for all the genes we selected are summarized in this <a href="https://static.igem.org/mediawiki/2014/6/60/Goettingen2014-NumberS.pdf">file.</a></p> | ||
Revision as of 10:16, 17 October 2014
Project
Wet lab
Approach
Our experimental approach can be summarized as follows:
1. Organisms and surface proteins selection
2. Surface protein amplification and cloning
3. Yeast two-hybrid assay with an existing peptide library
4. Peptide functionalization
Results summary
These are the genes and the organisms we selected:
| Number | Gene | Organism |
|---|---|---|
| 1 | mp65 | N/A |
| 2 | tos1 | N/A |
| 3 | sim1 | N/A |
| 4 | ssr1 | N/A |
| 5 | pir4 | N/A |
| 6 | scw4 | N/A |
| 7 | pir3 | N/A |
| 8 | utr2 | N/A |
| 9 | crf2full | A. fumigatus |
| 10 | crf2act | A. fumigatus |
| 11 | bgleno | A. fumigatus |
| 12 | ecm33 | A. fumigatus |
| 13 | sun1 | A. fumigatus |
| 14 | als1 | N/A |
| 15 | als3 | N/A |
| 16 | roda | A. fumigatus |
| 17 | roda | A. nidulans |
| 18 | xlna | A. nidulans |
| 19 | npc2 | A. nidulans |
| 20 | sho1 | A. nidulans |
| 21 | mid2 | S. cerevisiae |
| 22 | sed1 | S. cerevisiae |
| 23 | prm1 b | A. fumigatus |
| 24 | crf1 | A. fumigatus |
| 25 | ama1 | T. gondii |
| 26 | eglc | A. fumigatus |
| 27 | cwp1 | S. cerevisiae |
| 28 | cwp2 | S. cerevisiae |
| 29 | tir4 | S. cerevisiae |
| 30 | rom5 | T. gondii |
| 31 | rom4 | T. gondii |
| 32 | gra7 | T. gondii |
| 33 | eglc | A. nidulans |
| 34 | tir1 | S. cerevisiae |
| 35 | bgleex | A. fumigatus |
The results for all the genes we selected are summarized in this file.
