Team:SF Bay Area DIYbio/Notebook
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<p><b>September 2014</b></p><ul> | <p><b>September 2014</b></p><ul> | ||
- | <li>Cloning 7 casein gBlocks, | + | <li><a href="https://2014.igem.org/cloning7caesin">Cloning 7 casein gBlocks, <b>12 Sep 2014</b></a><br> |
<li>Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S<br> | <li>Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S<br> | ||
<li>Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014<br> | <li>Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014<br> |
Revision as of 23:05, 16 October 2014
WELCOME TO iGEM 2014!Your team has been approved and you are ready to start the iGEM season!
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Notebook
General Protocols
- Electra Cloning Protocol
- DNA Handling Protocol
- Media:Zymo E. coli competent cells transformation protocol.pdf
- Media:D4025i.pdf (Zyppy plasmid midiprep protocol)
- Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf
General Documentation Information
June 2014
August 2014
September 2014
- Cloning 7 casein gBlocks, 12 Sep 2014
- Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S
- Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
- Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014
October 2014
- Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2
- Cloning human alpha casein S1, 04Oct2014 and on
- Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014