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Team:Goettingen/project overview/project wetlab
From 2014.igem.org
(Difference between revisions)
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<th>AIG (derived from AH109)</th> | <th>AIG (derived from AH109)</th> | ||
<th>Y2H (interaction partners)</th> | <th>Y2H (interaction partners)</th> | ||
| - | |||
| - | |||
| - | |||
| - | |||
</tr> | </tr> | ||
| Line 127: | Line 123: | ||
<th>Gene</th> | <th>Gene</th> | ||
<th>Organism</th> | <th>Organism</th> | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
<th>pIGZ (p1170 from diploid cells for E.coli)</th> | <th>pIGZ (p1170 from diploid cells for E.coli)</th> | ||
<th>pIGY (p1170 from E.coli for Y187)</th> | <th>pIGY (p1170 from E.coli for Y187)</th> | ||
| Line 136: | Line 129: | ||
<th>PepTag</th> | <th>PepTag</th> | ||
</tr> | </tr> | ||
| - | <tr><td>1</td><td>X</td></tr> | + | |
| - | <tr><td>1</td><td>X</td></tr> | + | <tr><td>1</td><td>mp65</td><td></td><td>X</td></tr> |
| + | <tr><td>1</td><td>mp65</td><td></td><td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>1</td><td>X</td></tr> | + | <tr><td>1</td><td>mp65</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>1</td><td>X</td></tr> | + | <tr><td>1</td><td>mp65</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>2</td><td>X</td></tr> | + | <tr><td>2</td><td>tos1</td><td></td><td>X</td></tr> |
| - | <tr><td>2</td><td>X</td></tr> | + | <tr><td>2</td><td>tos1</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>2</td><td>X</td></tr> | + | <tr><td>2</td><td>tos1</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>2</td><td>X</td></tr> | + | <tr><td>2</td><td>tos1</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>3</td><td>X</td></tr> | + | <tr><td>3</td><td>sim1</td><td></td><td>X</td></tr> |
| - | <tr><td>3</td><td>X</td></tr> | + | <tr><td>3</td><td>sim1</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>3</td><td>X</td></tr> | + | <tr><td>3</td><td>sim1</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>3</td><td>X</td></tr> | + | <tr><td>3</td><td>sim1</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>4</td><td>X</td></tr> | + | <tr><td>4</td><td>ssr1</td><td></td><td>X</td></tr> |
| - | <tr><td>4</td><td>X</td></tr> | + | <tr><td>4</td><td>ssr1</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>4</td><td>X</td></tr> | + | <tr><td>4</td><td>ssr1</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>4</td><td>X</td></tr> | + | <tr><td>4</td><td>ssr1</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X | <td>X | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>5</td><td>X</td></tr> | + | <tr><td>5</td><td>pir4</td><td></td><td>X</td></tr> |
| - | <tr><td>5</td><td>X</td></tr> | + | <tr><td>5</td><td>pir4</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>5</td><td>X</td></tr> | + | <tr><td>5</td><td>pir4</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>5</td><td>X</td></tr> | + | <tr><td>5</td><td>pir4</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X | <td>X | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>6</td><td>X</td></tr> | + | <tr><td>6</td><td>scw4</td><td></td><td>X</td></tr> |
| - | <tr><td>6</td><td>X</td></tr> | + | <tr><td>6</td><td>scw4</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>6</td><td>X</td></tr> | + | <tr><td>6</td><td>scw4</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>6</td><td>X</td></tr> | + | <tr><td>6</td><td>scw4</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>7</td><td>N/A</td></tr> | + | <tr><td>7</td><td>pir3</td><td></td><td>N/A</td></tr> |
| - | <tr><td>7</td><td>N/A</td></tr> | + | <tr><td>7</td><td>pir3</td><td></td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>7</td><td>N/A</td></tr> | + | <tr><td>7</td><td>pir3</td><td></td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>7</td><td>N/A</td></tr> | + | <tr><td>7</td><td>pir3</td><td></td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>8</td><td>N/A</td></tr> | + | <tr><td>8</td><td>utr2</td><td></td><td>N/A</td></tr> |
| - | <tr><td>8</td><td>N/A</td></tr> | + | <tr><td>8</td><td>utr2</td><td></td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>8</td><td>N/A</td></tr> | + | <tr><td>8</td><td>utr2</td><td></td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>8</td><td>N/A</td></tr> | + | <tr><td>8</td><td>utr2</td><td></td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>9</td><td>X</td></tr> | + | <tr><td>9</td><td>crf2full</td><td>A. fumigatus</td><td>X</td></tr> |
| - | <tr><td>9</td><td>X</td></tr> | + | <tr><td>9</td><td>crf2full</td><td>A. fumigatus</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>9</td><td>X</td></tr> | + | <tr><td>9</td><td>crf2full</td><td>A. fumigatus</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>9</td><td>X</td></tr> | + | <tr><td>9</td><td>crf2full</td><td>A. fumigatus</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>10</td><td>X</td></tr> | + | <tr><td>10</td><td>crf2act</td><td>A. fumigatus</td><td>X</td></tr> |
| - | <tr><td>10</td><td>X</td></tr> | + | <tr><td>10</td><td>crf2act</td><td>A. fumigatus</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>10</td><td>X</td></tr> | + | <tr><td>10</td><td>crf2act</td><td>A. fumigatus</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>10</td><td>X</td></tr> | + | <tr><td>10</td><td>crf2act</td><td>A. fumigatus</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>11</td><td>N/A</td></tr> | + | <tr><td>11</td><td>bglEno</td><td>A. fumigatus</td><td>N/A</td></tr> |
| - | <tr><td>11</td><td>N/A</td></tr> | + | <tr><td>11</td><td>bglEno</td><td>A. fumigatus</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>11</td><td>N/A</td></tr> | + | <tr><td>11</td><td>bglEno</td><td>A. fumigatus</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>11</td><td>N/A</td></tr> | + | <tr><td>11</td><td>bglEno</td><td>A. fumigatus</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>12</td><td>X</td></tr> | + | <tr><td>12</td><td>ecm33</td><td>A. fumigatus</td><td>X</td></tr> |
| - | <tr><td>12</td><td>X</td></tr> | + | <tr><td>12</td><td>ecm33</td><td>A. fumigatus</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>12</td><td>X</td></tr> | + | <tr><td>12</td><td>ecm33</td><td>A. fumigatus</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>12</td><td>X</td></tr> | + | <tr><td>12</td><td>ecm33</td><td>A. fumigatus</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>13</td><td>X</td></tr> | + | <tr><td>13</td><td>sun1</td><td>A. fumigatus</td><td>X</td></tr> |
| - | <tr><td>13</td><td>X</td></tr> | + | <tr><td>13</td><td>sun1</td><td>A. fumigatus</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>13</td><td>X</td></tr> | + | <tr><td>13</td><td>sun1</td><td>A. fumigatus</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>13</td><td>X</td></tr> | + | <tr><td>13</td><td>sun1</td><td>A. fumigatus</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X | <td>X | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>14</td><td>X</td></tr> | + | <tr><td>14</td><td>als1</td><td></td><td>X</td></tr> |
| - | <tr><td>14</td><td>X</td></tr> | + | <tr><td>14</td><td>als1</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>14</td><td>X</td></tr> | + | <tr><td>14</td><td>als1</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>14</td><td>X</td></tr> | + | <tr><td>14</td><td>als1</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>15</td><td>X</td></tr> | + | <tr><td>15</td><td>als3</td><td></td><td>X</td></tr> |
| - | <tr><td>15</td><td>X</td></tr> | + | <tr><td>15</td><td>als3</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>15</td><td>X</td></tr> | + | <tr><td>15</td><td>als3</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>15</td><td>X</td></tr> | + | <tr><td>15</td><td>als3</td><td></td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>16</td><td>N/A</td></tr> | + | <tr><td>16</td><td>rodA</td><td>A. fumigatus</td><td>N/A</td></tr> |
| - | <tr><td>16</td><td>N/A</td></tr> | + | <tr><td>16</td><td>rodA</td><td>A. fumigatus</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>16</td><td>N/A</td></tr> | + | <tr><td>16</td><td>rodA</td><td>A. fumigatus</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>16</td><td>N/A</td></tr> | + | <tr><td>16</td><td>rodA</td><td>A. fumigatus</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>17</td><td>X</td></tr> | + | <tr><td>17</td><td>rodA</td><td>A. nidulans</td><td>X</td></tr> |
| - | <tr><td>17</td><td>X</td></tr> | + | <tr><td>17</td><td>rodA</td><td>A. nidulans</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>17</td><td>X</td></tr> | + | <tr><td>17</td><td>rodA</td><td>A. nidulans</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>17</td><td>X</td></tr> | + | <tr><td>17</td><td>rodA</td><td>A. nidulans</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>18</td><td>N/A</td></tr> | + | <tr><td>18</td><td>xlnA</td><td>A. nidulans</td><td>N/A</td></tr> |
| - | <tr><td>18</td><td>N/A</td></tr> | + | <tr><td>18</td><td>xlnA</td><td>A. nidulans</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>18</td><td>N/A</td></tr> | + | <tr><td>18</td><td>xlnA</td><td>A. nidulans</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>18</td><td>N/A</td></tr> | + | <tr><td>18</td><td>xlnA</td><td>A. nidulans</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>19</td><td>X</td></tr> | + | <tr><td>19</td><td>npc2</td><td>A. nidulans</td><td>X</td></tr> |
| - | <tr><td>19</td><td>X</td></tr> | + | <tr><td>19</td><td>npc2</td><td>A. nidulans</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>19</td><td>X</td></tr> | + | <tr><td>19</td><td>npc2</td><td>A. nidulans</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>19</td><td>X</td></tr> | + | <tr><td>19</td><td>npc2</td><td>A. nidulans</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>20</td><td>N/A</td></tr> | + | <tr><td>20</td><td>sho1</td><td>A. nidulans</td><td>N/A</td></tr> |
| - | <tr><td>20</td><td>N/A</td></tr> | + | <tr><td>20</td><td>sho1</td><td>A. nidulans</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>20</td><td>N/A</td></tr> | + | <tr><td>20</td><td>sho1</td><td>A. nidulans</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>20</td><td>N/A</td></tr> | + | <tr><td>20</td><td>sho1</td><td>A. nidulans</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>21</td><td>X</td></tr> | + | <tr><td>21</td><td>MID2</td><td>S. cerevisiae</td><td>X</td></tr> |
| - | <tr><td>21</td><td>X</td></tr> | + | <tr><td>21</td><td>MID2</td><td>S. cerevisiae</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>21</td><td>X</td></tr> | + | <tr><td>21</td><td>MID2</td><td>S. cerevisiae</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>21</td><td>X</td></tr> | + | <tr><td>21</td><td>MID2</td><td>S. cerevisiae</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>22</td><td>N/A</td></tr> | + | <tr><td>22</td><td>SED1</td><td>S. cerevisiae</td><td>N/A</td></tr> |
| - | <tr><td>22</td><td>N/A</td></tr> | + | <tr><td>22</td><td>SED1</td><td>S. cerevisiae</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>22</td><td>N/A</td></tr> | + | <tr><td>22</td><td>SED1</td><td>S. cerevisiae</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>22</td><td>N/A</td></tr> | + | <tr><td>22</td><td>SED1</td><td>S. cerevisiae</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>23</td><td>N/A</td></tr> | + | <tr><td>23</td><td>prm1 B</td><td>A. fumigatus</td><td>N/A</td></tr> |
| - | <tr><td>23</td><td>N/A</td></tr> | + | <tr><td>23</td><td>prm1 B</td><td>A. fumigatus</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>23</td><td>N/A</td></tr> | + | <tr><td>23</td><td>prm1 B</td><td>A. fumigatus</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>23</td><td>N/A</td></tr> | + | <tr><td>23</td><td>prm1 B</td><td>A. fumigatus</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>24</td><td>X</td></tr> | + | <tr><td>24</td><td>crf1</td><td>A. fumigatus</td><td>X</td></tr> |
| - | <tr><td>24</td><td>X</td></tr> | + | <tr><td>24</td><td>crf1</td><td>A. fumigatus</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>24</td><td>X</td></tr> | + | <tr><td>24</td><td>crf1</td><td>A. fumigatus</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>24</td><td>X</td></tr> | + | <tr><td>24</td><td>crf1</td><td>A. fumigatus</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>25</td><td>X</td></tr> | + | <tr><td>25</td><td>ama1</td><td>T. gondii</td><td>X</td></tr> |
| - | <tr><td>25</td><td>X</td></tr> | + | <tr><td>25</td><td>ama1</td><td>T. gondii</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>25</td><td>X</td></tr> | + | <tr><td>25</td><td>ama1</td><td>T. gondii</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
| - | <tr><td>25</td><td>X</td></tr> | + | <tr><td>25</td><td>ama1</td><td>T. gondii</td><td>X</td></tr> |
<td>X</td></tr> | <td>X</td></tr> | ||
<td>X</td></tr> | <td>X</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>26</td><td>N/A</td></tr> | + | <tr><td>26</td><td>eglC</td><td>A. fumigatus</td><td>N/A</td></tr> |
| - | <tr><td>26</td><td>N/A</td></tr> | + | <tr><td>26</td><td>eglC</td><td>A. fumigatus</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>26</td><td>N/A</td></tr> | + | <tr><td>26</td><td>eglC</td><td>A. fumigatus</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>26</td><td>N/A</td></tr> | + | <tr><td>26</td><td>eglC</td><td>A. fumigatus</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>27</td><td>N/A</td></tr> | + | <tr><td>27</td><td>CWP1</td><td>S. cerevisiae</td><td>N/A</td></tr> |
| - | <tr><td>27</td><td>N/A</td></tr> | + | <tr><td>27</td><td>CWP1</td><td>S. cerevisiae</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>27</td><td>N/A</td></tr> | + | <tr><td>27</td><td>CWP1</td><td>S. cerevisiae</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>27</td><td>N/A</td></tr> | + | <tr><td>27</td><td>CWP1</td><td>S. cerevisiae</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>28</td><td>N/A</td></tr> | + | <tr><td>28</td><td>CWP2</td><td>S. cerevisiae</td><td>N/A</td></tr> |
| - | <tr><td>28</td><td>N/A</td></tr> | + | <tr><td>28</td><td>CWP2</td><td>S. cerevisiae</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>28</td><td>N/A</td></tr> | + | <tr><td>28</td><td>CWP2</td><td>S. cerevisiae</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>28</td><td>N/A</td></tr> | + | <tr><td>28</td><td>CWP2</td><td>S. cerevisiae</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>29</td><td>N/A</td></tr> | + | <tr><td>29</td><td>TIR4</td><td>S. cerevisiae</td><td>N/A</td></tr> |
| - | <tr><td>29</td><td>N/A</td></tr> | + | <tr><td>29</td><td>TIR4</td><td>S. cerevisiae</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>29</td><td>N/A</td></tr> | + | <tr><td>29</td><td>TIR4</td><td>S. cerevisiae</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>29</td><td>N/A</td></tr> | + | <tr><td>29</td><td>TIR4</td><td>S. cerevisiae</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>30</td><td>N/A</td></tr> | + | <tr><td>30</td><td>rom5</td><td>T. gondii</td><td>N/A</td></tr> |
| - | <tr><td>30</td><td>N/A</td></tr> | + | <tr><td>30</td><td>rom5</td><td>T. gondii</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>30</td><td>N/A</td></tr> | + | <tr><td>30</td><td>rom5</td><td>T. gondii</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>30</td><td>N/A</td></tr> | + | <tr><td>30</td><td>rom5</td><td>T. gondii</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>31</td><td>N/A</td></tr> | + | <tr><td>31</td><td>rom4</td><td>T. gondii</td><td>N/A</td></tr> |
| - | <tr><td>31</td><td>N/A</td></tr> | + | <tr><td>31</td><td>rom4</td><td>T. gondii</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>31</td><td>N/A</td></tr> | + | <tr><td>31</td><td>rom4</td><td>T. gondii</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>31</td><td>N/A</td></tr> | + | <tr><td>31</td><td>rom4</td><td>T. gondii</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>32</td><td>N/A</td></tr> | + | <tr><td>32</td><td>gra7</td><td>T. gondii</td><td>N/A</td></tr> |
| - | <tr><td>32</td><td>N/A</td></tr> | + | <tr><td>32</td><td>gra7</td><td>T. gondii</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>32</td><td>N/A</td></tr> | + | <tr><td>32</td><td>gra7</td><td>T. gondii</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>32</td><td>N/A</td></tr> | + | <tr><td>32</td><td>gra7</td><td>T. gondii</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>33</td><td>N/A</td></tr> | + | <tr><td>33</td><td>eglC</td><td>A. nidulans</td><td>N/A</td></tr> |
| - | <tr><td>33</td><td>N/A</td></tr> | + | <tr><td>33</td><td>eglC</td><td>A. nidulans</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>33</td><td>N/A</td></tr> | + | <tr><td>33</td><td>eglC</td><td>A. nidulans</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>33</td><td>N/A</td></tr> | + | <tr><td>33</td><td>eglC</td><td>A. nidulans</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>34</td><td>N/A</td></tr> | + | <tr><td>34</td><td>TIR1</td><td>S. cerevisiae</td><td>N/A</td></tr> |
| - | <tr><td>34</td><td>N/A</td></tr> | + | <tr><td>34</td><td>TIR1</td><td>S. cerevisiae</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>34</td><td>N/A</td></tr> | + | <tr><td>34</td><td>TIR1</td><td>S. cerevisiae</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>34</td><td>N/A</td></tr> | + | <tr><td>34</td><td>TIR1</td><td>S. cerevisiae</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| - | <tr><td>35</td><td>N/A</td></tr> | + | <tr><td>35</td><td>bglEex</td><td>A. fumigatus</td><td>N/A</td></tr> |
| - | <tr><td>35</td><td>N/A</td></tr> | + | <tr><td>35</td><td>bglEex</td><td>A. fumigatus</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>35</td><td>N/A</td></tr> | + | <tr><td>35</td><td>bglEex</td><td>A. fumigatus</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
| - | <tr><td>35</td><td>N/A</td></tr> | + | <tr><td>35</td><td>bglEex</td><td>A. fumigatus</td><td>N/A</td></tr> |
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td>N/A</td></tr> | <td>N/A</td></tr> | ||
<td> | <td> | ||
</td></tr> | </td></tr> | ||
| + | |||
Revision as of 12:45, 16 October 2014
Project
Wet lab
Approach
Our experimental approach can be summarized as follows:
1. Organism and surface protein selection
2. Surface protein amplification and cloning
3. Yeast two-hybrid assay with an existing peptide library
4. Peptide functionalization
Results summary
| Number | Gene | Organism | pIGA (pDONR+GOI in E.coli) | pIGB (pDEST+GOI for AH109) | AIG (derived from AH109) | Y2H (interaction partners) |
|---|---|---|---|---|---|---|
| 1 | mp65 | N/A | X | X | X | X |
| 2 | tos1 | N/A | X | X | X | X |
| 3 | sim1 | N/A | X | X | X | X |
| 4 | ssr1 | N/A | X | X | X | X |
| 5 | pir4 | N/A | X | X | X | X |
| 6 | scw4 | N/A | X | X | X | X |
| 7 | pir3 | N/A | X | X | N/A | N/A |
| 8 | utr2 | N/A | X | X | N/A | N/A |
| 9 | crf2full | A. fumigatus | X | X | X | X |
| 10 | crf2act | A. fumigatus | X | X | X | X |
| 11 | bglEno | A. fumigatus | X | N/A | N/A | N/A |
| 12 | ecm33 | A. fumigatus | X | X | X | X |
| 13 | sun1 | A. fumigatus | X | X | X | X |
| 14 | als1 | N/A | X | X | X | X |
| 15 | als3 | N/A | X | X | X | X |
| 16 | rodA | A. fumigatus | X | X | N/A | N/A |
| 17 | rodA | A. nidulans | X | X | X | X |
| 18 | xlnA | A. nidulans | X | X | X | X |
| 19 | npc2 | A. nidulans | X | X | X | X |
| 20 | sho1 | A. nidulans | X | X | X | X |
| 21 | MID2 | S. cerevisiae | X | X | X | X |
| 22 | SED1 | S. cerevisiae | X | X | X | X |
| 23 | prm1 B | A. fumigatus | X | X | N/A | N/A |
| 24 | crf1 | A. fumigatus | X | X | X | X |
| 25 | ama1 | T. gondii | X | X | X | X |
| 26 | eglC | A. fumigatus | X | N/A | N/A | N/A |
| 27 | CWP1 | S. cerevisiae | X | X | N/A | N/A |
| 28 | CWP2 | S. cerevisiae | X | X | N/A | N/A |
| 29 | TIR4 | S. cerevisiae | X | N/A | N/A | N/A |
| 30 | rom5 | T. gondii | X | N/A | N/A | N/A |
| 31 | rom4 | T. gondii | X | N/A | N/A | N/A |
| 32 | gra7 | T. gondii | X | N/A | N/A | N/A |
| 33 | eglC | A. nidulans | X | N/A | N/A | N/A |
| 34 | TIR1 | S. cerevisiae | X | X | N/A | N/A |
| 35 | bglEex | A. fumigatus | X | X | N/A | N/A |
| Number | Gene | Organism | pIGZ (p1170 from diploid cells for E.coli) | pIGY (p1170 from E.coli for Y187) | YIG (derived from Y187) | PepTag |
|---|---|---|---|---|---|---|
| 1 | mp65 | X | ||||
| 1 | mp65 | X | X | |||
| 1 | mp65 | X | X | X | ||
| 1 | mp65 | X | X | X | ||
| 2 | tos1 | X | ||||
| 2 | tos1 | X | X | |||
| 2 | tos1 | X | X | X | ||
| 2 | tos1 | X | X | X | ||
| 3 | sim1 | X | ||||
| 3 | sim1 | X | X | |||
| 3 | sim1 | X | X | X | ||
| 3 | sim1 | X | X | X | ||
| 4 | ssr1 | X | ||||
| 4 | ssr1 | X | X | |||
| 4 | ssr1 | X | X | X | ||
| 4 | ssr1 | X | X | X | X | |
| 5 | pir4 | X | ||||
| 5 | pir4 | X | X | |||
| 5 | pir4 | X | X | X | ||
| 5 | pir4 | X | X | X | X | |
| 6 | scw4 | X | ||||
| 6 | scw4 | X | X | |||
| 6 | scw4 | X | X | X | ||
| 6 | scw4 | X | X | X | ||
| 7 | pir3 | N/A | ||||
| 7 | pir3 | N/A | N/A | |||
| 7 | pir3 | N/A | N/A | N/A | ||
| 7 | pir3 | N/A | N/A | N/A | ||
| 8 | utr2 | N/A | ||||
| 8 | utr2 | N/A | N/A | |||
| 8 | utr2 | N/A | N/A | N/A | ||
| 8 | utr2 | N/A | N/A | N/A | ||
| 9 | crf2full | A. fumigatus | X | |||
| 9 | crf2full | A. fumigatus | X | X | ||
| 9 | crf2full | A. fumigatus | X | X | X | |
| 9 | crf2full | A. fumigatus | X | X | X | |
| 10 | crf2act | A. fumigatus | X | |||
| 10 | crf2act | A. fumigatus | X | X | ||
| 10 | crf2act | A. fumigatus | X | X | X | |
| 10 | crf2act | A. fumigatus | X | X | X | |
| 11 | bglEno | A. fumigatus | N/A | |||
| 11 | bglEno | A. fumigatus | N/A | N/A | ||
| 11 | bglEno | A. fumigatus | N/A | N/A | N/A | |
| 11 | bglEno | A. fumigatus | N/A | N/A | N/A | |
| 12 | ecm33 | A. fumigatus | X | |||
| 12 | ecm33 | A. fumigatus | X | X | ||
| 12 | ecm33 | A. fumigatus | X | X | X | |
| 12 | ecm33 | A. fumigatus | X | X | X | |
| 13 | sun1 | A. fumigatus | X | |||
| 13 | sun1 | A. fumigatus | X | X | ||
| 13 | sun1 | A. fumigatus | X | X | X | |
| 13 | sun1 | A. fumigatus | X | X | X | X |
| 14 | als1 | X | ||||
| 14 | als1 | X | X | |||
| 14 | als1 | X | X | X | ||
| 14 | als1 | X | X | X | ||
| 15 | als3 | X | ||||
| 15 | als3 | X | X | |||
| 15 | als3 | X | X | X | ||
| 15 | als3 | X | X | X | ||
| 16 | rodA | A. fumigatus | N/A | |||
| 16 | rodA | A. fumigatus | N/A | N/A | ||
| 16 | rodA | A. fumigatus | N/A | N/A | N/A | |
| 16 | rodA | A. fumigatus | N/A | N/A | N/A | |
| 17 | rodA | A. nidulans | X | |||
| 17 | rodA | A. nidulans | X | X | ||
| 17 | rodA | A. nidulans | X | X | X | |
| 17 | rodA | A. nidulans | X | X | X | |
| 18 | xlnA | A. nidulans | N/A | |||
| 18 | xlnA | A. nidulans | N/A | N/A | ||
| 18 | xlnA | A. nidulans | N/A | N/A | N/A | |
| 18 | xlnA | A. nidulans | N/A | N/A | N/A | |
| 19 | npc2 | A. nidulans | X | |||
| 19 | npc2 | A. nidulans | X | X | ||
| 19 | npc2 | A. nidulans | X | X | X | |
| 19 | npc2 | A. nidulans | X | X | X | |
| 20 | sho1 | A. nidulans | N/A | |||
| 20 | sho1 | A. nidulans | N/A | N/A | ||
| 20 | sho1 | A. nidulans | N/A | N/A | N/A | |
| 20 | sho1 | A. nidulans | N/A | N/A | N/A | |
| 21 | MID2 | S. cerevisiae | X | |||
| 21 | MID2 | S. cerevisiae | X | X | ||
| 21 | MID2 | S. cerevisiae | X | X | X | |
| 21 | MID2 | S. cerevisiae | X | X | X | |
| 22 | SED1 | S. cerevisiae | N/A | |||
| 22 | SED1 | S. cerevisiae | N/A | N/A | ||
| 22 | SED1 | S. cerevisiae | N/A | N/A | N/A | |
| 22 | SED1 | S. cerevisiae | N/A | N/A | N/A | |
| 23 | prm1 B | A. fumigatus | N/A | |||
| 23 | prm1 B | A. fumigatus | N/A | N/A | ||
| 23 | prm1 B | A. fumigatus | N/A | N/A | N/A | |
| 23 | prm1 B | A. fumigatus | N/A | N/A | N/A | |
| 24 | crf1 | A. fumigatus | X | |||
| 24 | crf1 | A. fumigatus | X | X | ||
| 24 | crf1 | A. fumigatus | X | X | X | |
| 24 | crf1 | A. fumigatus | X | X | X | |
| 25 | ama1 | T. gondii | X | |||
| 25 | ama1 | T. gondii | X | X | ||
| 25 | ama1 | T. gondii | X | X | X | |
| 25 | ama1 | T. gondii | X | X | X | |
| 26 | eglC | A. fumigatus | N/A | |||
| 26 | eglC | A. fumigatus | N/A | N/A | ||
| 26 | eglC | A. fumigatus | N/A | N/A | N/A | |
| 26 | eglC | A. fumigatus | N/A | N/A | N/A | |
| 27 | CWP1 | S. cerevisiae | N/A | |||
| 27 | CWP1 | S. cerevisiae | N/A | N/A | ||
| 27 | CWP1 | S. cerevisiae | N/A | N/A | N/A | |
| 27 | CWP1 | S. cerevisiae | N/A | N/A | N/A | |
| 28 | CWP2 | S. cerevisiae | N/A | |||
| 28 | CWP2 | S. cerevisiae | N/A | N/A | ||
| 28 | CWP2 | S. cerevisiae | N/A | N/A | N/A | |
| 28 | CWP2 | S. cerevisiae | N/A | N/A | N/A | |
| 29 | TIR4 | S. cerevisiae | N/A | |||
| 29 | TIR4 | S. cerevisiae | N/A | N/A | ||
| 29 | TIR4 | S. cerevisiae | N/A | N/A | N/A | |
| 29 | TIR4 | S. cerevisiae | N/A | N/A | N/A | |
| 30 | rom5 | T. gondii | N/A | |||
| 30 | rom5 | T. gondii | N/A | N/A | ||
| 30 | rom5 | T. gondii | N/A | N/A | N/A | |
| 30 | rom5 | T. gondii | N/A | N/A | N/A | |
| 31 | rom4 | T. gondii | N/A | |||
| 31 | rom4 | T. gondii | N/A | N/A | ||
| 31 | rom4 | T. gondii | N/A | N/A | N/A | |
| 31 | rom4 | T. gondii | N/A | N/A | N/A | |
| 32 | gra7 | T. gondii | N/A | |||
| 32 | gra7 | T. gondii | N/A | N/A | ||
| 32 | gra7 | T. gondii | N/A | N/A | N/A | |
| 32 | gra7 | T. gondii | N/A | N/A | N/A | |
| 33 | eglC | A. nidulans | N/A | |||
| 33 | eglC | A. nidulans | N/A | N/A | ||
| 33 | eglC | A. nidulans | N/A | N/A | N/A | |
| 33 | eglC | A. nidulans | N/A | N/A | N/A | |
| 34 | TIR1 | S. cerevisiae | N/A | |||
| 34 | TIR1 | S. cerevisiae | N/A | N/A | ||
| 34 | TIR1 | S. cerevisiae | N/A | N/A | N/A | |
| 34 | TIR1 | S. cerevisiae | N/A | N/A | N/A | |
| 35 | bglEex | A. fumigatus | N/A | |||
| 35 | bglEex | A. fumigatus | N/A | N/A | ||
| 35 | bglEex | A. fumigatus | N/A | N/A | N/A | |
| 35 | bglEex | A. fumigatus | N/A | N/A | N/A |
