Team:Goettingen/project overview/project wetlab

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Revision as of 12:32, 16 October 2014

Project


ZH

Wet lab

Approach

Our experimental approach can be summarized as follows:


  1. 1. Organism and surface protein selection

  2. 2. Surface protein amplification and cloning

  3. 3. Yeast two-hybrid assay with an existing peptide library

  4. 4. Peptide functionalization



Click to enlarge


Results summary


Number Gene Organism pIGA (pDONR+GOI in E.coli) pIGB (pDEST+GOI for AH109) AIG (derived from AH109) Y2H (interaction partners) pIGZ (p1170 from diploid cells for E.coli) pIGY (p1170 from E.coli for Y187) YIG (derived from Y187) PepTag
1mp65N/AXXXXXXX
2tos1N/AXXXXXXX
3sim1N/AXXXXXXX
4ssr1N/AXXXXXXXX
5pir4N/AXXXXXXXX
6scw4N/AXXXXXXX
7pir3N/AXXN/AN/AN/AN/AN/A
8utr2N/AXXN/AN/AN/AN/AN/A
9crf2fullA. fumigatusXXXXXXX
10crf2actA. fumigatusXXXXXXX
11bglEnoA. fumigatusXN/AN/AN/AN/AN/AN/A
12ecm33A. fumigatusXXXXXXX
13sun1A. fumigatusXXXXXXXX
14als1N/AXXXXXXX
15als3N/AXXXXXXX
16rodAA. fumigatusXXN/AN/AN/AN/AN/A
17rodAA. nidulansXXXXXXX
18xlnAA. nidulansXXXXN/AN/AN/A
19npc2A. nidulansXXXXXXX
20sho1A. nidulansXXXXN/AN/AN/A
21MID2S. cerevisiaeXXXXXXX
22SED1S. cerevisiaeXXXXN/AN/AN/A
23prm1 BA. fumigatusXXN/AN/AN/AN/AN/A
24crf1A. fumigatusXXXXXXX
25ama1T. gondiiXXXXXXX
26eglCA. fumigatusXN/AN/AN/AN/AN/AN/A
27CWP1S. cerevisiaeXXN/AN/AN/AN/AN/A
28CWP2S. cerevisiaeXXN/AN/AN/AN/AN/A
29TIR4S. cerevisiaeXN/AN/AN/AN/AN/AN/A
30rom5T. gondiiXN/AN/AN/AN/AN/AN/A
31rom4T. gondiiXN/AN/AN/AN/AN/AN/A
32gra7T. gondiiXN/AN/AN/AN/AN/AN/A
33eglCA. nidulansXN/AN/AN/AN/AN/AN/A
34TIR1S. cerevisiaeXXN/AN/AN/AN/AN/A
35bglEexA. fumigatusXXN/AN/AN/AN/AN/A




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