Team:Goettingen/project overview/project wetlab

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<h2 id="wlresults">Results summary</h2>
<h2 id="wlresults">Results summary</h2>
-
<h3>Organism and surface protein selection</h3>
 
-
<h3>Surface protein amplification and cloning</h3>
 
-
<h3>Yeast two-hybrid assay with an existing peptide library</h3>
 
-
<h3>Peptide functionalization</h3>
 
-
<br /><br />
+
 
 +
<br />
 +
<table>
 +
<tr>
 +
<th>Number</th>
 +
<th>Gene</th>
 +
<th>Organism</th>
 +
<th>pIGA (pDONR+GOI in E.coli)</th>
 +
<th>pIGB (pDEST+GOI for AH109)</th>
 +
<th>AIG (derived from AH109)</th>
 +
<th>Y2H (interaction partners)</th>
 +
<th>pIGZ (p1170 from diploid cells for E.coli)</th>
 +
<th>pIGY (p1170 from E.coli for Y187)</th>
 +
<th>YIG (derived from Y187)</th>
 +
<th>PepTag</th>
 +
</tr>
 +
 
 +
<tr><td>1</td><td>mp65</td><td>N/A</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>
 +
</td></tr>
 +
<tr><td>2</td><td>tos1</td><td>N/A</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>
 +
</td></tr>
 +
<tr><td>3</td><td>sim1</td><td>N/A</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>
 +
</td></tr>
 +
<tr><td>4</td><td>ssr1</td><td>N/A</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X
 +
</td></tr>
 +
<tr><td>5</td><td>pir4</td><td>N/A</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X
 +
</td></tr>
 +
<tr><td>6</td><td>scw4</td><td>N/A</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>
 +
</td></tr>
 +
<tr><td>7</td><td>pir3</td><td>N/A</td><td>X</td><td>X</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>
 +
</td></tr>
 +
<tr><td>8</td><td>utr2</td><td>N/A</td><td>X</td><td>X</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>
 +
</td></tr>
 +
<tr><td>9</td><td>crf2full</td><td>A. fumigatus</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>
 +
</td></tr>
 +
<tr><td>10</td><td>crf2act</td><td>A. fumigatus</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>
 +
</td></tr>
 +
<tr><td>11</td><td>bglEno</td><td>A. fumigatus</td><td>X</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>
 +
</td></tr>
 +
<tr><td>12</td><td>ecm33</td><td>A. fumigatus</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>
 +
</td></tr>
 +
<tr><td>13</td><td>sun1</td><td>A. fumigatus</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X
 +
</td></tr>
 +
<tr><td>14</td><td>als1</td><td>N/A</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>
 +
</td></tr>
 +
<tr><td>15</td><td>als3</td><td>N/A</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>
 +
</td></tr>
 +
<tr><td>16</td><td>rodA</td><td>A. fumigatus</td><td>X</td><td>X</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>
 +
</td></tr>
 +
<tr><td>17</td><td>rodA</td><td>A. nidulans</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>
 +
</td></tr>
 +
<tr><td>18</td><td>xlnA</td><td>A. nidulans</td><td>X</td><td>X</td><td>X</td><td>X</td><td>N/A</td><td>N/A</td><td>N/A</td><td>
 +
</td></tr>
 +
<tr><td>19</td><td>npc2</td><td>A. nidulans</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>
 +
</td></tr>
 +
<tr><td>20</td><td>sho1</td><td>A. nidulans</td><td>X</td><td>X</td><td>X</td><td>X</td><td>N/A</td><td>N/A</td><td>N/A</td><td>
 +
</td></tr>
 +
<tr><td>21</td><td>MID2</td><td>S. cerevisiae</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>
 +
</td></tr>
 +
<tr><td>22</td><td>SED1</td><td>S. cerevisiae</td><td>X</td><td>X</td><td>X</td><td>X</td><td>N/A</td><td>N/A</td><td>N/A</td><td>
 +
</td></tr>
 +
<tr><td>23</td><td>prm1 B</td><td>A. fumigatus</td><td>X</td><td>X</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>
 +
</td></tr>
 +
<tr><td>24</td><td>crf1</td><td>A. fumigatus</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>
 +
</td></tr>
 +
<tr><td>25</td><td>ama1</td><td>T. gondii</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>X</td><td>
 +
</td></tr>
 +
<tr><td>26</td><td>eglC</td><td>A. fumigatus</td><td>X</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>
 +
</td></tr>
 +
<tr><td>27</td><td>CWP1</td><td>S. cerevisiae</td><td>X</td><td>X</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>
 +
</td></tr>
 +
<tr><td>28</td><td>CWP2</td><td>S. cerevisiae</td><td>X</td><td>X</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>
 +
</td></tr>
 +
<tr><td>29</td><td>TIR4</td><td>S. cerevisiae</td><td>X</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>
 +
</td></tr>
 +
<tr><td>30</td><td>rom5</td><td>T. gondii</td><td>X</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>
 +
</td></tr>
 +
<tr><td>31</td><td>rom4</td><td>T. gondii</td><td>X</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>
 +
</td></tr>
 +
<tr><td>32</td><td>gra7</td><td>T. gondii</td><td>X</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>
 +
</td></tr>
 +
<tr><td>33</td><td>eglC</td><td>A. nidulans</td><td>X</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>
 +
</td></tr>
 +
<tr><td>34</td><td>TIR1</td><td>S. cerevisiae</td><td>X</td><td>X</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>
 +
</td></tr>
 +
<tr><td>35</td><td>bglEex</td><td>A. fumigatus</td><td>X</td><td>X</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>N/A</td><td>
 +
</td></tr>
 +
</table>
 +
 
 +
<br />
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Revision as of 12:31, 16 October 2014

Project


ZH

Wet lab

Approach

Our experimental approach can be summarized as follows:


  1. 1. Organism and surface protein selection

  2. 2. Surface protein amplification and cloning

  3. 3. Yeast two-hybrid assay with an existing peptide library

  4. 4. Peptide functionalization



Click to enlarge


Results summary


Number Gene Organism pIGA (pDONR+GOI in E.coli) pIGB (pDEST+GOI for AH109) AIG (derived from AH109) Y2H (interaction partners) pIGZ (p1170 from diploid cells for E.coli) pIGY (p1170 from E.coli for Y187) YIG (derived from Y187) PepTag
1mp65N/AXXXXXXX
2tos1N/AXXXXXXX
3sim1N/AXXXXXXX
4ssr1N/AXXXXXXXX
5pir4N/AXXXXXXXX
6scw4N/AXXXXXXX
7pir3N/AXXN/AN/AN/AN/AN/A
8utr2N/AXXN/AN/AN/AN/AN/A
9crf2fullA. fumigatusXXXXXXX
10crf2actA. fumigatusXXXXXXX
11bglEnoA. fumigatusXN/AN/AN/AN/AN/AN/A
12ecm33A. fumigatusXXXXXXX
13sun1A. fumigatusXXXXXXXX
14als1N/AXXXXXXX
15als3N/AXXXXXXX
16rodAA. fumigatusXXN/AN/AN/AN/AN/A
17rodAA. nidulansXXXXXXX
18xlnAA. nidulansXXXXN/AN/AN/A
19npc2A. nidulansXXXXXXX
20sho1A. nidulansXXXXN/AN/AN/A
21MID2S. cerevisiaeXXXXXXX
22SED1S. cerevisiaeXXXXN/AN/AN/A
23prm1 BA. fumigatusXXN/AN/AN/AN/AN/A
24crf1A. fumigatusXXXXXXX
25ama1T. gondiiXXXXXXX
26eglCA. fumigatusXN/AN/AN/AN/AN/AN/A
27CWP1S. cerevisiaeXXN/AN/AN/AN/AN/A
28CWP2S. cerevisiaeXXN/AN/AN/AN/AN/A
29TIR4S. cerevisiaeXN/AN/AN/AN/AN/AN/A
30rom5T. gondiiXN/AN/AN/AN/AN/AN/A
31rom4T. gondiiXN/AN/AN/AN/AN/AN/A
32gra7T. gondiiXN/AN/AN/AN/AN/AN/A
33eglCA. nidulansXN/AN/AN/AN/AN/AN/A
34TIR1S. cerevisiaeXXN/AN/AN/AN/AN/A
35bglEexA. fumigatusXXN/AN/AN/AN/AN/A




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