Team:SF Bay Area DIYbio/Notebook
From 2014.igem.org
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CR_062214_humanKappaCasein_001 <br> | CR_062214_humanKappaCasein_001 <br> | ||
- | August 2014 | + | <p>August 2014 |
- | + | </p> | |
CLONE_082514_humKappaCasein(Kex+)<br> | CLONE_082514_humKappaCasein(Kex+)<br> | ||
Growth Curve Experiment <br> | Growth Curve Experiment <br> | ||
+ | <p> | ||
September 2014 | September 2014 | ||
- | + | </p> | |
Cloning 7 casein gBlocks, 12Sep2014 and on<br> | Cloning 7 casein gBlocks, 12Sep2014 and on<br> | ||
Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S<br> | Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S<br> | ||
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Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014<br> | Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014<br> | ||
+ | <p> | ||
October 2014 | October 2014 | ||
- | + | </p> | |
Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2<br> | Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2<br> | ||
Cloning human alpha casein S1, 04Oct2014 and on<br> | Cloning human alpha casein S1, 04Oct2014 and on<br> |
Revision as of 03:54, 14 October 2014
WELCOME TO iGEM 2014!Your team has been approved and you are ready to start the iGEM season!
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Notebook | ||||||||||||
General Protocols Electra Cloning ProtocolMedia:Zymo E. coli competent cells transformation protocol.pdf Media:D4025i.pdf (Zyppy plasmid midiprep protocol) Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf General Documentation Information Real Vegan Cheese Notebook Documentation GuidelinesReal Vegan Cheese Notebook Training Sign-off June 2014 CR_062214_humanKappaCasein_001August 2014 CLONE_082514_humKappaCasein(Kex+)Growth Curve Experiment September 2014 Cloning 7 casein gBlocks, 12Sep2014 and onTroubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014 October 2014 Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2Cloning human alpha casein S1, 04Oct2014 and on Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014 |