Team:Goettingen/project overview/project biobrick

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<td>Tool to tag yeast proteins with GFP</td>
<td>Tool to tag yeast proteins with GFP</td>
<td style="font-size:80%" height=100>Use this plasmid to enter your protein of interest into BamH1 or BgIII restriction site. It is tagged to GFP. The plasmid selection is done by TRP1.</td>
<td style="font-size:80%" height=100>Use this plasmid to enter your protein of interest into BamH1 or BgIII restriction site. It is tagged to GFP. The plasmid selection is done by TRP1.</td>
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<td>4304</td>
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<td>4685</td>
<td>Cedric Bloetz</td>
<td>Cedric Bloetz</td>

Revision as of 10:48, 2 October 2014

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BioBricks


Code Name Description Length (bp) Created by
K1430000 Yeast 2-micron
Mutated 2-micron for plasmid amplification in yeast cells. The 2 micron sequence was mutated that internal restriction sites (for Pre/-Suffix enzymes) are lost.
1343 Cedric Bloetz
K1430001 Yeast LEU2 gene Selective marker for auxotrophic strains of yeast after transforamtion with plasmid that contains this gene. 1095 Cedric Bloetz
K1430002 TRP1 gene from yeast This part is a fusion of promoter and terminator in front/back of the TRP1 of Saccharomyces cerevisiae 1501 Cedric Bloetz
K1430003 Yeast TRIP1 gene Selective marker for auxotrophic strains of yeast after transforamtion with plasmid that contains this gene. 675 Cedric Bloetz
K1430004 LEU2 gene from yeast This part is a fusion of promoter and terminator in front/back of the LEU2 of S. cerevisiae 1921 Cedric Bloetz
K1430005 GFP tag for yeast proteins The BamHI site in front of the GFP allows to tag proteins in yeast with GFP. 2953 Cedric Bloetz
K1430006 mRFP tag for yeast proteins The BamHI site in front of the mRFP allows to tag proteins in yeast with mRFP. 3334 Cedric Bloetz
K1430007 Tool to tag yeast proteins with GFP Use this plasmid to enter your protein of interest into BamH1 or BgIII restriction site. It is tagged to GFP. The plasmid selection is done by TRP1. 4685 Cedric Bloetz
K1430008 Tool to tag yeast proteins with mRFP Use this plasmid to enter your protein of interest into BamH1 or BgIII restriction site. It is tagged to mRFP. The plasmid selection is done by LEU2. 4304 Cedric Bloetz
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