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Team:Goettingen/project overview/project drylab results
From 2014.igem.org
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| - | <center><table style="width: | + | <center><table style="width:700px"> |
<tr style="font-weight:bold;"> | <tr style="font-weight:bold;"> | ||
<td>Peptide</td> | <td>Peptide</td> | ||
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<td>Energy profile</td> | <td>Energy profile</td> | ||
</tr> | </tr> | ||
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<tr> | <tr> | ||
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<td><a href="https://static.igem.org/mediawiki/2014/1/1e/Goettingen-Prey3-input.txt" target="1">Download</a></td> | <td><a href="https://static.igem.org/mediawiki/2014/1/1e/Goettingen-Prey3-input.txt" target="1">Download</a></td> | ||
<td style="font-size:80%;" height=100>Interacting with Sim1 protein of <i>Candida albicans</i></td> | <td style="font-size:80%;" height=100>Interacting with Sim1 protein of <i>Candida albicans</i></td> | ||
| - | <td style="font-size:80%;"K17-E42</td> | + | <td style="font-size:80%;">K17-E42</td> |
| - | <td style="font-size:80%;"QMEAN</td> | + | <td style="font-size:80%;">QMEAN</td> |
| - | <td style="font-size:80%;"-7053.966797</td> | + | <td style="font-size:80%;">-7053.966797</td> |
<td><a href="https://static.igem.org/mediawiki/2014/3/37/GoettingenPrey3-profile.txt" target="1">Download</a></td> | <td><a href="https://static.igem.org/mediawiki/2014/3/37/GoettingenPrey3-profile.txt" target="1">Download</a></td> | ||
</tr> | </tr> | ||
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<td><a href="https://static.igem.org/mediawiki/2014/6/6e/Goettingen-Prey4-1.png" target="1"><img src="https://static.igem.org/mediawiki/2014/6/6e/Goettingen-Prey4-1.png" width=100></a></td> | <td><a href="https://static.igem.org/mediawiki/2014/6/6e/Goettingen-Prey4-1.png" target="1"><img src="https://static.igem.org/mediawiki/2014/6/6e/Goettingen-Prey4-1.png" width=100></a></td> | ||
<td><a href="https://static.igem.org/mediawiki/2014/1/1b/Goettingen-Prey4-1.txt" target="1">Download</a></td> | <td><a href="https://static.igem.org/mediawiki/2014/1/1b/Goettingen-Prey4-1.txt" target="1">Download</a></td> | ||
| - | <td style="font-size:80%;" height=100>Interacting with Ssr1(CAGL0H06413g) protein of <i>Candida glabrata</i></td> | + | <td style="font-size:80%;"> height=100>Interacting with Ssr1(CAGL0H06413g) protein of <i>Candida glabrata</i></td> |
| - | <td style="font-size:80%;"K17-E44</td> | + | <td style="font-size:80%;">K17-E44</td> |
| - | <td style="font-size:80%;"QMEAN</td> | + | <td style="font-size:80%;">QMEAN</td> |
| - | <td style="font-size:80%;"-7246.956055</td> | + | <td style="font-size:80%;">-7246.956055</td> |
<td><a href="https://static.igem.org/mediawiki/2014/0/0b/GoettingenPrey4-1-profile.txt" target="1">Download</a></td> | <td><a href="https://static.igem.org/mediawiki/2014/0/0b/GoettingenPrey4-1-profile.txt" target="1">Download</a></td> | ||
</tr> | </tr> | ||
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<td><a href="https://static.igem.org/mediawiki/2014/8/86/Goettingen-Prey5.txt" target="1">Download</a></td> | <td><a href="https://static.igem.org/mediawiki/2014/8/86/Goettingen-Prey5.txt" target="1">Download</a></td> | ||
<td style="font-size:80%;" height=100>Interacting with Pir4 (CAGL0I06160g) protein of <i>Candida glabrata</i></td> | <td style="font-size:80%;" height=100>Interacting with Pir4 (CAGL0I06160g) protein of <i>Candida glabrata</i></td> | ||
| - | <td style="font-size:80%;"K17-E44</td> | + | <td style="font-size:80%;">K17-E44</td> |
| - | <td style="font-size:80%;"QMEAN</td> | + | <td style="font-size:80%;">QMEAN</td> |
| - | <td style="font-size:80%;"-7501.071777</td> | + | <td style="font-size:80%;">-7501.071777</td> |
<td><a href="https://static.igem.org/mediawiki/2014/1/10/GoettingenPrey5-profile.txt" target="1">Download</a></td> | <td><a href="https://static.igem.org/mediawiki/2014/1/10/GoettingenPrey5-profile.txt" target="1">Download</a></td> | ||
</tr> | </tr> | ||
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<td><a href="https://static.igem.org/mediawiki/2014/8/8e/Goettingen-Prey13-input.txt" target="1">Download</a></td> | <td><a href="https://static.igem.org/mediawiki/2014/8/8e/Goettingen-Prey13-input.txt" target="1">Download</a></td> | ||
<td style="font-size:80%;" height=100>Interacting with Sun1 protein of <i>Aspergillus fumigatus</i></td> | <td style="font-size:80%;" height=100>Interacting with Sun1 protein of <i>Aspergillus fumigatus</i></td> | ||
| - | <td style="font-size:80%;"K17-E42</td> | + | <td style="font-size:80%;>">K17-E42</td> |
| - | <td style="font-size:80%;"QMEAN</td> | + | <td style="font-size:80%;">QMEAN</td> |
<td style="font-size:80%;">-7729.056152</td> | <td style="font-size:80%;">-7729.056152</td> | ||
<td><a href="https://static.igem.org/mediawiki/2014/e/eb/GoettingenPrey13-profile.txt" target="1">Download</a></td> | <td><a href="https://static.igem.org/mediawiki/2014/e/eb/GoettingenPrey13-profile.txt" target="1">Download</a></td> | ||
Revision as of 09:34, 29 September 2014
Project
Dry lab
Results summary:
We obtained working structural models for our peptides inside their scaffolds. The following table is a summary of them. Please note that these models are not definitive, since they were obtained by homology modeling and loop refinement. Their main purpose is to give us an idea if the calculated energy profile matches our assumption that the peptide is being exposed to the exterior of the scaffold.
Peptide
PNG*
PDB
Description
Peptide location
QMEAN
Overall DOPE score
Energy profile
Prey 3
Download
Interacting with Sim1 protein of Candida albicans
K17-E42
QMEAN
-7053.966797
Download
Prey 4.1
Download
height=100>Interacting with Ssr1(CAGL0H06413g) protein of Candida glabrata
K17-E44
QMEAN
-7246.956055
Download
Prey 5
Download
Interacting with Pir4 (CAGL0I06160g) protein of Candida glabrata
K17-E44
QMEAN
-7501.071777
Download
Prey 13
Download
Interacting with Sun1 protein of Aspergillus fumigatus
K17-E42
QMEAN
-7729.056152
Download
Prey 15
Download
Interacting with Als3 protein of Candida albicans
K17-E42
-7671.660156
Download
*The peptide is shown in red and the scaffold in blue.
This is peptide IGP4 that shows interaction with ssr1 protein from Candida glabrata
We obtained working structural models for our peptides inside their scaffolds. The following table is a summary of them. Please note that these models are not definitive, since they were obtained by homology modeling and loop refinement. Their main purpose is to give us an idea if the calculated energy profile matches our assumption that the peptide is being exposed to the exterior of the scaffold.
| Peptide | PNG* | PDB | Description | Peptide location | QMEAN | Overall DOPE score | Energy profile |
| Prey 3 | |
Download | Interacting with Sim1 protein of Candida albicans | K17-E42 | QMEAN | -7053.966797 | Download |
| Prey 4.1 | |
Download | height=100>Interacting with Ssr1(CAGL0H06413g) protein of Candida glabrata | K17-E44 | QMEAN | -7246.956055 | Download |
| Prey 5 | |
Download | Interacting with Pir4 (CAGL0I06160g) protein of Candida glabrata | K17-E44 | QMEAN | -7501.071777 | Download |
| Prey 13 | |
Download | Interacting with Sun1 protein of Aspergillus fumigatus | K17-E42 | QMEAN | -7729.056152 | Download |
| Prey 15 | |
Download | Interacting with Als3 protein of Candida albicans | K17-E42 | -7671.660156 | Download |
This is peptide IGP4 that shows interaction with ssr1 protein from Candida glabrata
