Team:Paris Saclay/Notebook/September/9
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+ | photo 1 GS (8 clones) ans cad (8 clones) | ||
+ | photo 2 LS (8 clones) | ||
After these results I choose some clones to do a liquid culture | After these results I choose some clones to do a liquid culture |
Revision as of 13:22, 14 September 2014
Contents |
Lab Work
By Mélanie
D-Lemon scent
extraction of TOPO plasmide with PS
Extraction with a Dna Kit extraction
[photo]
I failed
PCR
Transformation show some clone so I do a PCR.
component | volume |
---|---|
H2O | 41.5μl |
buffer | 5μl |
dNTPs | 1μl |
Primer 1 | 1μl |
Primer 2 | 1μl |
bacteria | (about 1µl = 1 colony) |
Dream taq | 0.5μl |
Primer used:
Pu Pr (universal primer)
Cycle step | Temperature | Time | Cycle |
---|---|---|---|
Bacteria lysis |
95°C |
5 min |
1 |
Denaturation | 94°C | 30 s | 25 |
Annealing | 50°C | 25 s | 25 |
Extension | 72°C | 1 min | 25 |
Final extension | 72°C | 10 min | 1 |
Final extension | 8°C | hold | 1 |
photo 1 GS (8 clones) ans cad (8 clones) photo 2 LS (8 clones)
After these results I choose some clones to do a liquid culture