Team:Hong Kong-CUHK/safety.html

From 2014.igem.org

(Difference between revisions)
(Created page with "<html> <head> <script src="js/jquery-1.11.1.min.js?action=raw&ctype=text/javascript"></script> <script src="js/bootstrap.min.js?action=raw&ctype=text/javascri...")
m
Line 8: Line 8:
     </head>
     </head>
     <body>
     <body>
-
        <div class="row">
+
<script type="text/javascript">
-
            <!--Nav Bar -->
+
$(document).ready(function(){
-
            <nav class="col-xs-3 bs-docs-sidebar">
+
$("#globalWrapper").load("safety_template.html&action=raw");
-
                <ul id="sidebar" class="nav nav-stacked fixed">
+
});
-
                    <li class="active">
+
</script>
-
                        <a href="#group-training">Training</a>
+
-
                        <ul class="nav nav-stacked">
+
-
                            <li><a href="#subgroup-safety-training">Safety training received</a></li>
+
-
                            <li><a href="#subgroup-topics-learned">Topics learned</a></li>
+
-
                            <li><a href="#subgroup-safety-requirements">Safety requirements</a></li>
+
-
                        </ul>
+
-
                    </li>
+
-
                    <li>
+
-
                        <a href="#group-local-rules">Local Rules</a>
+
-
                        <ul class="nav nav-stacked">
+
-
                            <li><a href="#subgroup-person-in-charge-for-safety">Person in Charge for safety</a></li>
+
-
                            <li><a href="#subgroup-biosafety-guidelines">Biosafety guidelines</a></li>
+
-
                            <li><a href="#subgroup-government-regulations">Government regulations</a></li>
+
-
                        </ul>
+
-
                    </li>
+
-
                    <li>
+
-
                        <a href="#group-organisms-and-parts-used">Organisms and Parts used</a>
+
-
                        <ul class="nav nav-stacked">
+
-
                            <li><a href="#subgroup-species">Species</a></li>
+
-
                            <li><a href="#subgroup-parts">Parts</a></li>
+
-
                        </ul>
+
-
                    </li>
+
-
                    <li>
+
-
                        <a href="#group-risks-now">Risks of the Project Now</a>
+
-
                        <ul class="nav nav-stacked">
+
-
                            <li><a href="#subgroup-team-members">Team members or people in lab</a></li>
+
-
                            <li><a href="#subgroup-general-public">General public</a></li>
+
-
                            <li><a href="#subgroup-environment">Environment</a></li>
+
-
                            <li><a href="#subgroup-security">Security</a></li>
+
-
                            <li><a href="#subgroup-how-to-reduce">How to reduce</a></li>
+
-
                        </ul>
+
-
                    </li>
+
-
                    <li>
+
-
                        <a href="#group-risks-future">Risks of the Project Future</a>
+
-
                        <ul class="nav nav-stacked">
+
-
                            <li><a href="#subgroup-new-risks">New risks</a></li>
+
-
                            <li><a href="#subgroup-minimize-risk">Minimize risks</a></li>
+
-
                        </ul>
+
-
                    </li>
+
-
                </ul>
+
-
            </nav>
+
-
            <!--Main Content -->
+
-
            <div class="col-xs-9">
+
-
                <section id="group-training" class="group">
+
-
                    <h3>Your Training</h3>
+
-
                    <div id="subgroup-safety-training" class="subgroup well">
+
-
                        <h4>Have your team members received any safety training yet?</h4>
+
-
                        <p>Yes, we have already received safety training.</p>
+
-
                    </div>
+
-
                    <div id="subgroup-topics-learned" class="subgroup well">
+
-
                        <h4>Please briefly describe the topics that you learned about (or will learn about) in your safety training.</h4>
+
-
                        We have received Biosafety courses on:
+
-
                        <ul>
+
-
                            <li>Safety laws and policy</li>
+
-
                            <li>Fire safety and emergency procedures</li>
+
-
                            <li>Electrical safety</li>
+
-
                            <li>Safety information resources including MSDS</li>
+
-
                            <li>Chemical handling requirements and waste disposal procedures</li>
+
-
                            <li>Use of personal protective equipment</li>
+
-
                            <li>Practicing safe science</li>
+
-
                            <li>Use of fume cupboards</li>
+
-
                            <li>Principle of Biological Safety</li>
+
-
                            <li>Risk Group and Biosafety Level</li>
+
-
                            <li>Laboratory Practices</li>
+
-
                            <li>Bioaerosol</li>
+
-
                            <li>Biosafety Cabinet</li>
+
-
                            <li>Sterilization</li>
+
-
                            <li>Biowastes</li>
+
-
                            <li>Genetically Manipulated Organisms</li>
+
-
                        </ul>
+
-
                        In detail, regarding Biological and Related Safety, we have learned:
+
-
                        <ol>
+
-
                            <li>Risk-Grouping of Micro-organisms by Type</li>
+
-
                            <li>Biosafety Levels</li>
+
-
                            <li>Abbreviated Laboratory Biosafety Level Criteria</li>
+
-
                            <li>Handling Blood and Other "Products" of Human Origin</li>
+
-
                            <li>Biosafety Cabinets</li>
+
-
                            <li>Hazards from "Microbial" Aerosols</li>
+
-
                            <li>Work with Recombinant DNA</li>
+
-
                            <li>Treatment of Biological Waste and Spill Cleanup</li>
+
-
                            <li>Autoclave Safety and Disinfection</li>
+
-
                            <li>Clinical Waste Disposal</li>
+
-
                        </ol>
+
-
                    </div>
+
-
                    <div id="subgroup-safety-requirements" class="subgroup well">
+
-
                        <h4>Please give a link to the laboratory safety training requirements of your institution (college, university, community lab, etc). Or, if you cannot give a link, briefly describe the requirements.</h4>
+
-
                        <a href="http://www.cuhk.edu.hk/useo/so/training.html">http://www.cuhk.edu.hk/useo/so/training.html</a>
+
-
                    </div>
+
-
                </section>
+
-
                <section id="group-local-rules" class="group">
+
-
                    <h3>Your Local Rules and Regulations</h3>
+
-
                    <div id="subgroup-person-in-charge-for-safety" class="subgroup well">
+
-
                        <h4>Who is responsible for biological safety at your institution? (You might have an Institutional Biosafety Committee, an Office of Environmental Health and Safety, a single Biosafety Officer, or some other arrangement.) Have you discussed your project with them? Describe any concerns they raised, and any changes you made in your project based on your discussion.</h4>
+
-
                        Mr. YIP Sung Tat from the University Safety Office of the Chinese University of Hong Kong is one of the persons responsible for biological safety at our institution. <br/>
+
-
                        The University Safety Office have done a regular safety check of the lab on 7/3/2014 and the rectification has been carried out by department. <br />
+
-
                        We discussed our project with university safety office every year, for evaluation of the project safety issue.
+
-
                    </div>
+
-
                    <div id="subgroup-biosafety-guidelines" class="subgroup well">
+
-
                        <h4>What are the biosafety guidelines of your institution? Please give a link to these guidelines, or briefly describe them if you cannot give a link.</h4>
+
-
                        <a href="http://www.cuhk.edu.hk/useo/safety/ko_download/safetypolicy.pdf">http://www.cuhk.edu.hk/useo/safety/ko_download/safetypolicy.pdf</a>
+
-
                    </div>
+
-
                    <div id="subgroup-government-regulations" class="subgroup well">
+
-
                        <h4>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</h4>
+
-
                        <a href="http://www.chp.gov.hk/files/pdf/guidelines_on_biosafety_in_the_clinical_laboratory_2nd_edn.pdf">http://www.chp.gov.hk/files/pdf/guidelines_on_biosafety_in_the_clinical_laboratory_2nd_edn.pdf</a>
+
-
                    </div>
+
-
                </section>
+
-
                <section id="group-organisms-and-parts-used" class="group">
+
-
                    <h3>The Organisms and Parts that You Use</h3>
+
-
                    <div id="subgroup-species" class="subgroup">
+
-
                        <table class="table table-hover">
+
-
                            <thead>
+
-
                                <tr>
+
-
                                    <th>Species name</th>
+
-
                                    <th>Disease risk</th>
+
-
                                    <th>Source</th>
+
-
                                    <th>Usage</th>
+
-
                                </tr>
+
-
                            </thead>
+
-
                            <tbody>
+
-
                                <tr>
+
-
                                    <td>Escherichia coli (K-12 Derivatives)</td>
+
-
                                    <td>None</td>
+
-
                                    <td>Loo's Laboratory (in CUHK)</td>
+
-
                                    <td>Transformation and DNA extraction</td>
+
-
                                </tr>
+
-
                                <tr>
+
-
                                    <td>Azotobacter vinelandii (DJ)</td>
+
-
                                    <td>None</td>
+
-
                                    <td>Prof. Dennis R Dean's Laboratory</td>
+
-
                                    <td>Transformation and DNA extraction</td>
+
-
                                </tr>
+
-
                                <tr>
+
-
                                    <td>Aquifex aeolicus</td>
+
-
                                    <td>None</td>
+
-
                                    <td>Dr. Marianne Guiral's Laboratory</td>
+
-
                                    <td>DNA extraction</td>
+
-
                                </tr>
+
-
                            </tbody>
+
-
                        </table>
+
-
                    </div>
+
-
                    <div id="subgroup-parts" class="subgroup">
+
-
                        <table class="table table-hover">
+
-
                            <thead>
+
-
                                <tr>
+
-
                                    <th>Part number/name</th>
+
-
                                    <th>Natural function</th>
+
-
                                    <th>Usage</th>
+
-
                                </tr>
+
-
                            </thead>
+
-
                            <tbody>
+
-
                                <tr>
+
-
                                    <td>CFS_EV</td>
+
-
                                    <td>Enhancement vector</td>
+
-
                                    <td>Enhancement vector for carbon fixation system</td>
+
-
                                </tr>
+
-
                                <tr>
+
-
                                    <td>CFS_HAPV</td>
+
-
                                    <td>Hydrogenase accessory protein vector</td>
+
-
                                    <td>Hydrogenase accessory protein vector for carbon fixation system</td>
+
-
                                </tr>
+
-
                                <tr>
+
-
                                    <td>CFS_NAPV</td>
+
-
                                    <td>Nitrogenase accessory protein vector</td>
+
-
                                    <td>Nitrogenase accessory protein vector for carbon fixation system</td>
+
-
                                </tr>
+
-
                                <tr>
+
-
                                    <td>CFS_NHSPV</td>
+
-
                                    <td>Nitrogenase and hydrogenase structural protein vector</td>
+
-
                                    <td>&nbsp;</td>
+
-
                                </tr>
+
-
                                <tr>
+
-
                                    <td>CFS</td>
+
-
                                    <td>Functional biobrick for carbon fixation system</td>
+
-
                                    <td>&nbsp;</td>
+
-
                                </tr>
+
-
                                <tr>
+
-
                                    <td>IES1</td>
+
-
                                    <td>Inducible Expression system biobrick (1)</td>
+
-
                                    <td>Intermediate biobrick for Inducible expression system</td>
+
-
                                </tr>
+
-
                                <tr>
+
-
                                    <td>IES_ISNE</td>
+
-
                                    <td>Specific integration silencing nitrogenase expression</td>
+
-
                                    <td>Specific integration silencing nitrogenase expression for inducible expression system</td>
+
-
                                </tr>
+
-
                            </tbody>
+
-
                        </table>
+
-
                    </div>
+
-
                </section>
+
-
                <section id="group-risks-now" class="group">
+
-
                    <h3>Risks of Your Project Now</h3>
+
-
                    <div id="subgroup-team-members" class="subgroup well">
+
-
                        <h4>Risks to the safety and health of team members, or other people working in the lab</h4>
+
-
                        The organisms that we handle are all from Risk group 1, which are in low risk - generally do not cause disease in healthy adult humans. There should not be any severe risks if all steps are handled properly according to biological safety guidelines. On the other hand, there may be risks if the biological waste and spill are not treated properly, or if the recombinant DNA steps are not handled properly.
+
-
                    </div>
+
-
                    <div id="subgroup-general-public" class="subgroup well">
+
-
                        <h4>Risks to the safety and health of the general public (if any biological materials escaped from your lab)</h4>
+
-
                        As the organisms that we handle are in Risk group 1, and both Azotobacter and E. coli exist in natural environment, they will not give high risk to general public even if escaping from our lab. The modified protein system in our project is only dealing with common gas in atmosphere, such as CO<sub>2</sub>, H<sub>2</sub>, and CH<sub>4</sub>. So far as we planned, the production amount is far from sufficient to cause public danger.
+
-
                    </div>
+
-
                    <div id="subgroup-environment" class="subgroup well">
+
-
                        <h4>Risks to the environment (from waste disposal, or from materials escaping from your lab)</h4>
+
-
                        As the organisms that we handle are in Risk group 1, and both Azotobacter and E. coli exist in natural environment, they will not give high risk to natural environment even if escaping from our lab. The modified protein system in our project is only dealing with common gas in atmosphere, such as CO<sub>2</sub>, H<sub>2</sub>, and CH<sub>4</sub>. So far as we planned, the production amount is far from sufficient to cause environmental.
+
-
                    </div>
+
-
                    <div id="subgroup-security" class="subgroup well">
+
-
                        <h4>Risks to security through malicious mis-use by individuals, groups, or countries</h4>
+
-
                        Our project does not bear high risk to be maliciously mis-used by individuals, groups or countries, as it does not have any direct threat to human-being and human society.
+
-
                    </div>
+
-
                    <div id="subgroup-how-to-reduce" class="subgroup well">
+
-
                        <h4>What measures are you taking to reduce these risks?</h4>
+
-
                        We ensure that every member of the team has finished the lab safety training course before entering the lab. Teammates have been trained on proper handling of biological and chemical materials before doing lab work alone. In the lab, personal protective equipments (PPEs) are advised to wear according to requirement. Biological and chemical wastes are disposed properly according o guidelines, respectively. We have considered the safety issue when choosing organisms to use. Also, we communicate with supervisors during regular lab meetings, or whenever a problem comes into our concern.
+
-
                    </div>
+
-
                </section>
+
-
                <section id="group-risks-future" class="group">
+
-
                    <h3>Risks of Your Project in the Future</h3>
+
-
                    <div id="subgroup-new-risks" class="subgroup well">
+
-
                        <h4>What new risks might arise from your project's growth? (Consider the categories of risk listed in parts a-d of the previous question: lab workers, the general public, the environment, and malicious mis-uses.) Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</h4>
+
-
                        As far as we foresee, there will not be any severe new risks arising from the project's growth. Our project does not have any severe direct risk towards lab workers, general public, environment and malicious mis-uses.
+
-
                    </div>
+
-
                    <div id="subgroup-minimize-risk" class="subgroup well">
+
-
                        <h4>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks? (For example: auxotrophic chassis, physical containment, etc.) Such features are not required for an iGEM project, but many teams choose to explore them.</h4>
+
-
                        We designed certain promoters to regulate the protein expressions of our system. In the future, some self-control system design can be integrated in our project to help control the death of manipulated organism and the expression level of our system.
+
-
                    </div>
+
-
                </section>
+
-
            </div>
+
-
        </div>
+
-
        <div id="top-bar"></div>
+
     </body>
     </body>
</html>
</html>

Revision as of 04:49, 7 September 2014

Retrieved from "http://2014.igem.org/Team:Hong_Kong-CUHK/safety.html"