Team:Paris Saclay/Notebook/July/21

From 2014.igem.org

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*The new strains received
*The new strains received
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[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the Calendar]
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===4 - Electrophoresis===
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''by Fabio (process A) and Mathieu (process B and C)''
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[[File:LU000069.jpg|right]]
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[[File:LU000071.jpg|right]]
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[[File:LU000070.jpg|right]]
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We used 2µL of DNA of the following Biobricks in a 1% Agarose Gel.
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''The PCRs came from Mathias' manipulation made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 18th July].''
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'''''Process A'''''
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#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 J23119 Cl1]
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#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 J23119 Cl2]
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#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 J23106 Cl1]
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#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 J23100 Cl2]
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#PCR 1
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#PCR 2
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#PCR 3
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#PCR 4
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#PCR 5
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#PCR 6
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#PCR 7
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#PCR 8
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#PCR 9
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#PCR 10
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'''''Process B'''''
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#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 J23100 Cl1]
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#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 J23100 Cl2]
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#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 J23106 Cl1]
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#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 J23106 Cl2]
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#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 J23114 Cl1]
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#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 J23114 Cl2]
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'''''Process C'''''
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''Pooling and purifying PCR 9 and 10 from process A.''
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#PCR purified products. IPS70 / IPS71 of pOsV230 (Apramycin)
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#BT 340 (plasmid's flipase)
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'''Results:'''
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*'''A''': From 1 to 4: Success, DNAs have the expected size.
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*'''A''': From 5 to 12: Failure, No PCR products.
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*'''A''': Numbers 13 and 14: Success, PCR products have the expected size.
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*'''B''': All 6 extractions were successful.
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*'''C''': Number 1: successful concentration of pOsV230's PCR product.
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*'''C''': Number 2 had no migration.
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
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'''People there''':
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* Instructors and advisors: Solenne and Sylvie.
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* Students: Arnaud, Fabio, Juliette, Mathieu, Marie, Pierre, Romain, Sean and Terry.
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[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]

Revision as of 16:52, 21 July 2014

Contents

Monday 21st July

Lab Work

1 - Results: Transformation of supercompetent cells with CaCl2

by Romain

Strains transformed: MG1655, MG1655Z1. from Bacterial Cultures transformed the 18th July

Results: Nothing has grown.

2 - Oligo's design

by Romain

3 - Liquid Bacterial Culture

by Marie, Romain & Sean

  • DY330 pJBEI6409 with 10µl Cm in 10ml LB (x2)
  • BT340 Cm and Amp
  • The new strains received

4 - Electrophoresis

by Fabio (process A) and Mathieu (process B and C)

LU000069.jpg
LU000071.jpg
LU000070.jpg

We used 2µL of DNA of the following Biobricks in a 1% Agarose Gel. The PCRs came from Mathias' manipulation made the 18th July.

Process A

  1. J23119 Cl1
  2. J23119 Cl2
  3. J23106 Cl1
  4. J23100 Cl2
  5. PCR 1
  6. PCR 2
  7. PCR 3
  8. PCR 4
  9. PCR 5
  10. PCR 6
  11. PCR 7
  12. PCR 8
  13. PCR 9
  14. PCR 10

Process B

  1. J23100 Cl1
  2. J23100 Cl2
  3. J23106 Cl1
  4. J23106 Cl2
  5. J23114 Cl1
  6. J23114 Cl2

Process C

Pooling and purifying PCR 9 and 10 from process A.

  1. PCR purified products. IPS70 / IPS71 of pOsV230 (Apramycin)
  2. BT 340 (plasmid's flipase)

Results:

  • A: From 1 to 4: Success, DNAs have the expected size.
  • A: From 5 to 12: Failure, No PCR products.
  • A: Numbers 13 and 14: Success, PCR products have the expected size.
  • B: All 6 extractions were successful.
  • C: Number 1: successful concentration of pOsV230's PCR product.
  • C: Number 2 had no migration.

Protocol

People there:

  • Instructors and advisors: Solenne and Sylvie.
  • Students: Arnaud, Fabio, Juliette, Mathieu, Marie, Pierre, Romain, Sean and Terry.

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/July/21"