Team:Paris Saclay/Notebook/September/8
From 2014.igem.org
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====PCR with bacteria==== | ====PCR with bacteria==== | ||
+ | From the petri dish ([https://2014.igem.org/Team:Paris_Saclay/Notebook/September/5#Transformation made here], I select some clone to do a PCR : | ||
+ | clones 1-2-3 for cad | ||
+ | clones 4-5 for PS | ||
+ | clones 6 for LS | ||
+ | |||
+ | {| class="wikitable centre" width="80%" | ||
+ | |+ | ||
+ | |- | ||
+ | ! scope=col | Cycle step | ||
+ | ! scope=col | Temperature | ||
+ | ! scope=col | Time | ||
+ | ! scope=col | Cycle | ||
+ | |- | ||
+ | | width="25%" | | ||
+ | Initial denaturation | ||
+ | | width="25%" | | ||
+ | 94°C | ||
+ | | width="25%" | | ||
+ | 1 min | ||
+ | | width="25%" | | ||
+ | 1 | ||
+ | |- | ||
+ | | Denaturation | ||
+ | | 94°C | ||
+ | | 30 s | ||
+ | | 25 | ||
+ | |- | ||
+ | | Annealing | ||
+ | | 50°C | ||
+ | | 25 s | ||
+ | | 25 | ||
+ | |- | ||
+ | | Extension | ||
+ | | 72°C | ||
+ | | 1 min | ||
+ | | 25 | ||
+ | |- | ||
+ | | Final extension | ||
+ | | 72°C | ||
+ | | 10 min | ||
+ | | 1 | ||
+ | |- | ||
+ | | Final extension | ||
+ | | 8°C | ||
+ | | hold | ||
+ | | 1 | ||
+ | |} |
Revision as of 15:40, 9 September 2014
Contents |
Lab Work
D-Lemon Scent
Checking PCR
Electrophoresis of the PCR made Friday
[photo]
PCR with bacteria
From the petri dish (made here, I select some clone to do a PCR : clones 1-2-3 for cad clones 4-5 for PS clones 6 for LS
Cycle step | Temperature | Time | Cycle |
---|---|---|---|
Initial denaturation |
94°C |
1 min |
1 |
Denaturation | 94°C | 30 s | 25 |
Annealing | 50°C | 25 s | 25 |
Extension | 72°C | 1 min | 25 |
Final extension | 72°C | 10 min | 1 |
Final extension | 8°C | hold | 1 |