Team:Valencia UPV/Achievements
From 2014.igem.org
(Difference between revisions)
Alejovigno (Talk | contribs) |
|||
(16 intermediate revisions not shown) | |||
Line 16: | Line 16: | ||
<ul> | <ul> | ||
- | <li>Obtained pheromones being among the most abundant plant organic volatile compounds.<span class="red-bold">(Z)-11-hexadecen-1-ol</span> is certainly the most abundant one.</li> | + | <li>Obtained pheromones being among the most abundant plant organic volatile compounds.<span class="red-bold"> (Z)-11-hexadecen-1-ol</span> is certainly the most abundant one.</li> |
- | <li>Successful and functional assembly of each of the pheromone biosynthetic genes with the plant constitutive promoter P35S. Also multigenic assembly of all three transcription units in a single plasmid. <a href="https://2014.igem.org/Team:Valencia_UPV/Project/results/ | + | <li>Successful and functional assembly of each of the pheromone biosynthetic genes with the plant constitutive promoter P35S. Also multigenic assembly of all three transcription units in a single plasmid. <a href="https://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#biosyn" class="normal-link-page"> Results: Constructs-Biosynthesis</a></li> |
- | <li>Proof of our produced pheromones-insect interaction. <a href="https://2014.igem.org/Team:Valencia_UPV/Project/ | + | <li>Proof of our produced pheromones-insect interaction. <a href="https://2014.igem.org/Team:Valencia_UPV/Project/eag" class="normal-link-page">Results: Electroantennography</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
Line 25: | Line 25: | ||
<ul class="method"> | <ul class="method"> | ||
- | <li><a class="black-bold">A broad understanding of plant metabolism, by adapting and exploitation of a genome-scale model of Arabidopsis thaliana primary metabolism able to help us theoretically optimize the pheromone production Modeling: Pheromone Production</a> | + | <li><a class="black-bold">A broad understanding of plant metabolism, by adapting and exploitation of a genome-scale model of Arabidopsis thaliana primary metabolism able to help us theoretically optimize the pheromone production </a> <a href="https://2014.igem.org/Team:Valencia_UPV/Modeling/fba" class="normal-link-page"> Modeling: Pheromone Production</a> |
<ul> | <ul> | ||
- | <li>Adapting the AraGEM genome-scale model to our pheromone production pathway by branching the flux of our precursor metabolite Palmitic | + | <li>Adapting the AraGEM genome-scale model to our pheromone production pathway by branching the flux of our precursor metabolite Palmitic acid (16:0).</li> |
<li>Identifying the principal: i) cell compartments and scenarios of the plant metabolism, ii) flux bounds and constraints for each scenario, and iii) the interactions between chemical reactions and substrates related to our pheromone. </li> | <li>Identifying the principal: i) cell compartments and scenarios of the plant metabolism, ii) flux bounds and constraints for each scenario, and iii) the interactions between chemical reactions and substrates related to our pheromone. </li> | ||
<li>Exploring genetic conditions that could improve our pheromone production by Single gene Knock-out analysis. </li> | <li>Exploring genetic conditions that could improve our pheromone production by Single gene Knock-out analysis. </li> | ||
Line 43: | Line 43: | ||
<ul> | <ul> | ||
- | <li>Successful cloning of a trichome-specific promoter (PCPS2) from the genome of N tabacum and subsequent assembly with GFP as a reporter. <a href="https://2014.igem.org/Team:Valencia_UPV/Project/results/constructs# | + | <li>Successful cloning of a trichome-specific promoter (PCPS2) from the genome of <i>N. tabacum</i> and subsequent assembly with GFP as a reporter. <a href="https://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#phero" class="normal-link-page">Results: Constructs-Pheromone release</a></li> |
<li>Proof of the specificity of this promoter by GFP fluorescence detection. <a href="https://2014.igem.org/Team:Valencia_UPV/Project/results/trichome_expression" class="normal-link-page">Results: Trichome-specific expression</a></li> | <li>Proof of the specificity of this promoter by GFP fluorescence detection. <a href="https://2014.igem.org/Team:Valencia_UPV/Project/results/trichome_expression" class="normal-link-page">Results: Trichome-specific expression</a></li> | ||
- | <li>Assembly of each gene of the pheromones production pathway with PCPS2 promoter and multigenic assembly with all three transcription units in a single plasmid. <a href="https://2014.igem.org/Team:Valencia_UPV/Project/results/constructs# | + | <li>Assembly of each gene of the pheromones production pathway with PCPS2 promoter and multigenic assembly with all three transcription units in a single plasmid. <a href="https://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#phero" class="normal-link-page">Results: Constructs-Pheromone release</a></a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
Line 52: | Line 52: | ||
<ul class="method"> | <ul class="method"> | ||
- | <li><a class="black-bold">A genetic switch able to control gene expression ready to be implemented in the plant. | + | <li><a class="black-bold">A Simulation Environment for the pheromone diffusion and moth response behaviour to help us decide where and how much sexy plants we should put in the field. </a> <a href="https://2014.igem.org/Team:Valencia_UPV/Modeling/diffusion" class="normal-link-page"> Modeling: Pheromone Diffusion and Moths Response</a> |
+ | <ul> | ||
+ | <li>Implementing an approximation of the diffusion process by the heat diffusion equation and its numerical solution.</li> | ||
+ | <li>Incorporating an approximation of the moth response behavior including female pheromone release into the environment, and male pheromone concentration sensitivity (that allows the male to follow the trace of females).</li> | ||
+ | <li>Adding our sexy plants that produce and release pheromone triggering <i>mating disruption</i>.</li> | ||
+ | <li>Designing a simulation platform available to community that is useful for exploration of parameters and scenarios related with chemical ecology models.</li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </p> | ||
+ | <br/><br/> | ||
+ | |||
+ | |||
+ | <ul class="method"> | ||
+ | <li><a class="black-bold">A genetic switch able to control gene expression ready to be implemented in the plant.</a> | ||
<ul> | <ul> | ||
<li>Cloning of the coding sequence from the CUP2 transcription factor from S cerevisiae and assembly with the CaMV constitutive promoter P35S. <a href="https://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a></li> | <li>Cloning of the coding sequence from the CUP2 transcription factor from S cerevisiae and assembly with the CaMV constitutive promoter P35S. <a href="https://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a></li> | ||
- | <li>Creation of the Cupper-responsive chimeric promoter and assembly with Firefly luciferase gene as a reporter, P19 as a gene silencing suppressor, and Renilla luciferase as control for Luciferase expression assay.<a href="https://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a></li> | + | <li>Creation of the Cupper-responsive chimeric promoter and assembly with Firefly luciferase gene as a reporter, P19 as a gene silencing suppressor, and Renilla luciferase as control for Luciferase expression assay. <a href="https://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a></li> |
<li>Multigenic assembly comprising the CUP2 transcriptional unit with the chimeric promoter and reporter gene assembly. | <li>Multigenic assembly comprising the CUP2 transcriptional unit with the chimeric promoter and reporter gene assembly. | ||
<a href="https://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a></li> | <a href="https://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a></li> | ||
Line 64: | Line 77: | ||
<ul class="method"> | <ul class="method"> | ||
- | <li><a class="black-bold">A sterile and dark purple plant safe for living beings and the environment.<a href="https://2014.igem.org/Team:Valencia_UPV/Project/ | + | <li><a class="black-bold">A sterile and dark purple plant safe for living beings and the environment.<a href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosafety" class="normal-link-page">Biosafety</a> |
<ul> | <ul> | ||
- | <li>Multigenic assembly of two biosafety devices, comprising the Barnase (male-sterility) with one chromoprotein in each device, AmilCP or AmilGFP (identity preservation). <a href="https://2014.igem.org/Team:Valencia_UPV/Project/results/constructs# | + | <li>Multigenic assembly of two biosafety devices, comprising the Barnase (male-sterility) with one chromoprotein in each device, AmilCP or AmilGFP (identity preservation). <a href="https://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#biosafe" class="normal-link-page"> Results: Constructs-Biosafety</a></li> |
- | <li>Purple plant to preserve its identity expressing | + | <li>Purple plant to preserve its identity expressing SlANT1 and SlJAF13 transcription factors. <a href="https://2014.igem.org/Team:Valencia_UPV/Project/results/biosafety" class="normal-link-page"> Results: Biosafety</a></li> |
</ul> | </ul> | ||
Line 75: | Line 88: | ||
<p> | <p> | ||
- | <a class="black-bold">Diffusion and communication of the project with involved experts and stakeholders.<a href="https://2014.igem.org/Team:Valencia_UPV/policy/ | + | <a class="black-bold">Diffusion and communication of the project with involved experts and stakeholders.<a href="https://2014.igem.org/Team:Valencia_UPV/policy/overview" class="normal-link-page"> Policy and Practices</a></a> |
</p> | </p> | ||
Latest revision as of 03:52, 18 October 2014
Policy and Practices > Achievements
As it can bee observed surfing our wiki, at the end of this long road we have accomplished many positive results:
- A plant able to produce three insect sexual pheromones from moths to produce insects mating disruption, the Sexy Plant.: Results: Pheromone analysis
- Obtained pheromones being among the most abundant plant organic volatile compounds. (Z)-11-hexadecen-1-ol is certainly the most abundant one.
- Successful and functional assembly of each of the pheromone biosynthetic genes with the plant constitutive promoter P35S. Also multigenic assembly of all three transcription units in a single plasmid. Results: Constructs-Biosynthesis
- Proof of our produced pheromones-insect interaction. Results: Electroantennography
- A broad understanding of plant metabolism, by adapting and exploitation of a genome-scale model of Arabidopsis thaliana primary metabolism able to help us theoretically optimize the pheromone production Modeling: Pheromone Production
- Adapting the AraGEM genome-scale model to our pheromone production pathway by branching the flux of our precursor metabolite Palmitic acid (16:0).
- Identifying the principal: i) cell compartments and scenarios of the plant metabolism, ii) flux bounds and constraints for each scenario, and iii) the interactions between chemical reactions and substrates related to our pheromone.
- Exploring genetic conditions that could improve our pheromone production by Single gene Knock-out analysis.
- Obtaining optimal condition for the coupled yield of pheromone and biomass production as a function of photons consumption.
- A plant potentially able to release the produced pheromones into the environment.
- Successful cloning of a trichome-specific promoter (PCPS2) from the genome of N. tabacum and subsequent assembly with GFP as a reporter. Results: Constructs-Pheromone release
- Proof of the specificity of this promoter by GFP fluorescence detection. Results: Trichome-specific expression
- Assembly of each gene of the pheromones production pathway with PCPS2 promoter and multigenic assembly with all three transcription units in a single plasmid. Results: Constructs-Pheromone release
- A Simulation Environment for the pheromone diffusion and moth response behaviour to help us decide where and how much sexy plants we should put in the field. Modeling: Pheromone Diffusion and Moths Response
- Implementing an approximation of the diffusion process by the heat diffusion equation and its numerical solution.
- Incorporating an approximation of the moth response behavior including female pheromone release into the environment, and male pheromone concentration sensitivity (that allows the male to follow the trace of females).
- Adding our sexy plants that produce and release pheromone triggering mating disruption.
- Designing a simulation platform available to community that is useful for exploration of parameters and scenarios related with chemical ecology models.
- A genetic switch able to control gene expression ready to be implemented in the plant.
- Cloning of the coding sequence from the CUP2 transcription factor from S cerevisiae and assembly with the CaMV constitutive promoter P35S. Results: Construct-Switch
- Creation of the Cupper-responsive chimeric promoter and assembly with Firefly luciferase gene as a reporter, P19 as a gene silencing suppressor, and Renilla luciferase as control for Luciferase expression assay. Results: Construct-Switch
- Multigenic assembly comprising the CUP2 transcriptional unit with the chimeric promoter and reporter gene assembly. Results: Construct-Switch
- A sterile and dark purple plant safe for living beings and the environment.Biosafety
- Multigenic assembly of two biosafety devices, comprising the Barnase (male-sterility) with one chromoprotein in each device, AmilCP or AmilGFP (identity preservation). Results: Constructs-Biosafety
- Purple plant to preserve its identity expressing SlANT1 and SlJAF13 transcription factors. Results: Biosafety
Diffusion and communication of the project with involved experts and stakeholders. Policy and Practices